Exogenous application of spermidine and methyl jasmonate can mitigate salt stress in fenugreek (Trigonella foenum-graecum L.)

Abstract

Fenugreek (Trigonella foenum-graecum L.) has long been cultivated as both culinary and medicinal plant. The objective of this study was to investigate the effect of plant growth regulators such as spermidine (Spd) and methyl jasmonate (MeJA) on morpho-physiological and biochemical properties of fenugreek under saline conditions. A replicated greenhouse experiment in completely randomized block design was conducted with two levels of salinity (0.5 dS/m as control and 6 dS/m), three levels of spermidine (0, 1, 2 mM) and methyl jasmonate (0, 100, 200 µM), respectively. The results showed that salinity elevation reduced the leaf relative water content, leaf water potential, canopy-air temperature difference, photochemistry efficiency of photosystem II, and water-use efficiency of fenugreek. In contrast, salinity increased leaf thickness,greenness index, canopy temperature, leaf Na+, ash contents, malondialdehyde, other aldehydes, and electrolyte leakage. Moreover, Salinity stress reduced the amount of photosynthetic pigments, chlorophyll a/b ratio, total soluble protein, K+, maximum fluorescence, and K+/Na+ ratio in leaves. Elevation of water salinity, however, resulted in an increase in the activity of antioxidant enzymes (except superoxide dismutase), proline, and minimum fluorescence. When fenugreek was treated with 1 mM spermidine and/or 100 µM methyl jasmonate, the adverse effects of salinity stress were significantly mitigated, while the effects of these plant growth regulators on mitigating salinity stress were less pronounced at higher concentrations (2 mM spermidine and/or 200 µM methyl jasmonate). The current study suggests the application of 1 mM spermidine and/or 100 µM methyl jasmonate during the vegetative growth stage mitigating the adverse effects of salinity with an associated improvement in plant growth under moderate salinity (about 6 dS/m) when used for irrigation.