Canola Meal In Diets Research Articles

PSI-30 Effects of high canola meal diet with acidifiers supplementation on cecal microbiota of nursery pigs

Abstract Inclusion of canola meal (CM) in swine diets is typically limited stemming from the presence of glucosinolates (Gls), which can reduce growth performance and cause thyroid dysfunction in nursery pigs. While certain members of hindgut bacterial communities are capable of degrading dietary Gls, which would mitigate their detrimental effects, they have yet to be identified. Moreover, lowering the pH in the hindgut of the pig can break down Gls into less toxic compounds, potentially reducing harm. Thus, this study aimed to characterize major bacterial species populating the hindgut of nursery pigs fed a high CM diet supplemented with acidifier and encapsulated butyrate. A total of 315 nursery pigs weaned at 21 d old, average body weight (BW) 6.0 ± 1.2 kg, were assigned to one of five dietary treatments with 9 replicate pens (7 pigs/pen) in a randomized complete block design. The dietary treatments included PC: a corn-soybean meal-based diet with 20% CM, NC: a corn-soybean meal-based diet with 40% CM, NC+A: NC diet supplemented with an acidifier at 1,500 ppm, NC+B: NC diet supplemented with encapsulated butyrate at 500 ppm, and NC+A+B: NC diet supplemented with both an acidifier and encapsulated butyrate. All diets were formulated to meet or exceed the nutrient requirements of NRC (2012). At the end of the feeding trial (6 wk), one pig from each pen was euthanized to collect fresh cecal digesta. Samples were analyzed using the 16S rRNA gene through PCR-amplification of the V1-V3 regions from microbial genomic DNA, followed by Illumina MiSeq 2X300 sequencing. A comparative analysis of the highly represented taxonomic groups and operational taxonomic units (OTU) using non-parametric Kruskal-Wallis sum-rank test and Wilcoxon pairwise test identified candidate bacterial groups or species enriched across different diets. Bacillota was the predominant phylum across all diets, ranging from 83.2% to 86.3%. At the genus level, Lachnospiracea incertae sedis was less abundant in the NC diets with acidifier (Acidifier effect, P < 0.05), whereas Dialister was less in the NC diets with encapsulated butyrate (Butyrate effect, P < 0.05). The OTU Ssd-00001 (Lactobacillus amylovorus, 99.6%) tended to be greater in the cecal microbiota of pigs fed the NC diets with acidifier (Acidifier effect, P = 0.09). Interestingly, OTU Ssd-00188 (Agathobacter rectalis, 99.2%) tended to be higher in PC diet than in NC diet (P = 0.06), while its abundance was less in the cecal microbiota of pigs fed the NC diets with acidifier (Acidifier effect, P = 0.09). In conclusion, these results provide valuable insights into the microbiome of pigs fed high CM diets. Understanding the interplay between microbial communities and high CM diet with acidifier supplementation is crucial for optimizing swine diets and mitigating challenges associated with the antinutritional effects of dietary Gls from CM.

138 Growth performance and physiological response of nursery pigs fed a high canola meal diet with acidifiers

Abstract This study investigated the impact of feed additives aimed at reducing gut pH on the growth performance and physiological responses of nursery pigs when fed a high canola meal (CM) diet. A total of 315 nursery pigs, 6.0 ± 1.2 kg body weight (BW), were allotted to one of five dietary treatments with 9 replicates (7 pigs/pen) in a randomized complete block design. Dietary treatments were PC: corn-soybean meal-based diet with 20% CM, NC: corn-soybean meal-based diet with 40% CM; NC+A: NC diet with acidifier at 1,500 ppm, NC+B: NC diet with encapsulated butyrate at 500 ppm, and NC+A+B: NC diet with acidifier and encapsulated butyrate. All diets were formulated to meet or exceed the nutrient requirements of NRC (2012). All pigs received a common Phase 1 nursery diet during week 1 following weaning. Experimental diets were provided in Phase 2 (wk 2 and 3) and Phase 3 (wk 4 to 6). Individual pig BW and pen feed consumption were measured for each phase to calculate growth performance. At the conclusion of each phase, blood samples were collected from one pig per pen for analysis of serum triiodothyronine (T3) and thyroxine (T4) concentrations. The selected pig was euthanized within 24 h, and its organ weights and pH of digesta were measured. The results were analyzed using MIXED procedure in SAS with the pen as an experimental unit. There was no difference in BW and average daily gain throughout the overall period. However, PC treatment exhibited greater (P < 0.05) average daily feed intake in phase 3 by 18.2% and overall period by 13.4% compared with NC treatment and no difference with NC+A+B treatment. The NC+A and NC+A+B treatments tended to improve (P ≤ 0.10) gain to feed ratio in phase 3 by 17.9% and overall period by 16.1% compared with PC treatment, respectively. At the end of phase 3, the PC treatment tended (P < 0.10) to have greater serum T4 concentrations compared with NC and NC+A treatments (3.2 vs 2.5 and 2.4 ug/dL, respectively), whereas there was no difference between PC and NC+A+B treatments. In phase 3, the thyroid gland weight for PC treatment was less (P < 0.05) than for NC treatment (0.156 vs 0.202 g/kg), whereas there was no difference among PC, NC+B, and NC+A+B treatments. In phase 3, dietary encapsulated butyrate decreased (P < 0.05) the pH of jejunal digesta compared with PC treatment (6.28 vs 7.01), and dietary encapsulated butyrate treatments (NC+B and NC+A+B) tended to decrease (P < 0.10) the pH of hind-colonic digesta compared with the pH of PC treatment by 6.2% and 7.2%. In conclusion, dietary encapsulated butyrate in combination with acidifier may alleviate thyroid dysfunction that is anti-nutritional effect of high canola meal diets.

Production effects of extruded soybean meal replacing canola meal in the diet of lactating dairy cows

This study investigated the effects of extruded soybean meal (ESBM) in comparison with canola meal (CM) fed on an equivalent crude protein (CP) basis on lactational performance and ruminal fermentation of dairy cows. Following a 2-wk covariate period, 48 Holstein cows averaging (±SD): 146 ± 46 d in milk (DIM) and 43 ± 7 kg/d milk yield (MY) were assigned 1 of 2 treatment diets in a randomized complete block design experiment, which included a 2-wk period for dietary treatment adaptation before experimental data were collected. Following the adaptation period, samples and experimental data were collected for a total of 7 wk. Cows were blocked based on parity, DIM, and MY. Treatment diets contained 15.8% CM (containing 41.2% CP) or 13.2% ESBM (with 48.7% CP) of total mixed ration dry matter (DM), with similar inclusion of other feed ingredients. The CM diet was supplemented with canola oil, whereas the ESBM diet was supplemented with soybean hulls to achieve similar ether extract and neutral detergent fiber contents between the diets. Urea and rumen-protected Met and Lys were added to both diets to meet or exceed cow recommendations. Whole-ruminal digesta samples were collected from 10 (5 per treatment) ruminally cannulated cows. Eight cannulated cows were removed during the last week of the experiment to participate in another study. Treatment did not affect DM intake and MY or energy-corrected MY of the cows. Energy-corrected MY, apart from experimental wk 5, was similar between treatments. Apart from experimental wk 3 and 7, milk fat concentration and yield were greater for cows fed ESBM compared with CM. In multiparous cows only, milk true protein yield was greater for cows fed CM compared with ESBM. Ruminal concentration of total volatile fatty acids and the molar proportion of acetate were greater for ESBM, and propionate and valerate were greater in cows fed CM. Acetate to propionate ratio was greater for cows fed ESBM versus CM diet. Compared with the CM diet, the ESBM diet increased plasma concentrations of Ile, Leu, and Phe but not the sum of essential AA. Apparent total-tract digestibility of acid detergent fiber was greater in cows fed ESBM relative to CM. In this experiment, CM and ESBM included on an equal CP basis in the diet of dairy cows, resulted in similar DM intake, MY, and feed efficiency.

Growth performance, nutrient digestibility, antioxidant state, ileal histomorphometry, and cecal ecology of broilers fed on fermented canola meal with and without exogenous enzymes

This study was conducted to evaluate the effects of supplementation of exogenous enzymes in broiler diets that includes fermented canola meal on performance, nutrient digestibility, biochemical indication, antioxidative capacity, digestive enzyme activity, immune responses, and gut health. Five hundred 1-day-old Ross 308 broiler chicks were randomly allocated into five experimental groups (5 replicate/group), the first group: a control (CON) contained a basal diet, and the second to the fifth groups were fed diets as follows: containing 20% canola meal (CM), contains 20% fermented canola meal (FCM), contains 20% canola meal and exogenous enzymes at 0.02%/kg feed (ECM), and contains 20% fermented canola meal and exogenous enzymes at 0.02%/kg feed (EFC), respectively. At the finisher phase, the best body weight gain, feed conversion ratio, and nutrient utilization were associated with chickens fed EFC compared to other groups (P < 0.05). Total protein, albumin, alanine aminotransferase, and superoxide dismutase levels increased (P < 0.05), while cholesterol and malondialdehyde levels decreased in chickens fed on EFC. Likewise, there was a significant increase in the relative weight of the bursa of Fabricius and antibody titer against Newcastle disease, whereas the weight of abdominal fat decreased in the EFC group compared to other groups. Furthermore, there was a significant improvement in the activity of lipase and amylase enzymes (P < 0.05) in the EFC group. Fermented canola meal addition improved gut health (decreased Escherichia coli, increased Lactobacillus, and the highest values of villus height). Overall, these results confirmed that supplementing a fermented canola meal diet with exogenous enzymes improved growth performance through enhancing nutrient digestibility, immunity, antioxidant capacity, and gut health. Thus, adding enzymes to a diet containing fermented canola meal can be recommended as an alternative protein source that could be safely used to replace up to 20% soybean meal in broiler diets.

Ileal nutrient and energy digestibility of steam-exploded canola meal in cannulated grower pigs and total tract nutrient digestibility and growth performance of diets containing steam-exploded canola meal in weaned pigs

Canola meal is a relatively highly fibrous protein meal that could benefit from further processing to disrupt its fibre matrix, reduce anti-nutritional factors and increase nutrient availability. Two studies were conducted to determine effects of steam-explosion on digestibility of gross energy (GE), crude protein (CP) and amino acids (AA) in solvent-extracted Brassica napus canola meal in grower pigs and diet nutrient digestibility and growth performance in weaned pigs. Canola meal was steam-exploded at either 0.7 MPa (CM7) or 1.1 MPa (CM11) for 5 min. In study 1, 8 ileal-cannulated barrows (29 kg mean initial body weight) were fed 3 corn starch-based diets including 400 g non-steam-exploded canola meal (CM0), CM7 or CM11/kg and a N-free diet in a replicated 4 × 3 Youden square. The canola meal sample contained 361 g CP/kg and 17.2 g chemically available lysine/kg that was reduced to 14.1 g/kg in CM7 and 14.6 g/kg in CM11. Steam-explosion of canola meal at 0.7 MPa decreased (P < 0.05) the coefficient of apparent ileal digestibility (CAID) of dry matter (DM) and GE. Steam-explosion decreased (P < 0.05) the coefficient of standardised ileal digestibility (CSID) of CP and AA in canola meal except for tryptophan, proline and tyrosine. In study 2, four wheat-based diets were formulated to include 200 g/kg soybean meal (SBM), CM0, CM7 or CM11 and to provide 9.6 MJ net energy/kg and 12 g standardised ileal digestible lysine/kg. In total, 256 weaned pigs (10 kg mean initial body weight) were fed the diets for 4 weeks starting 2 weeks post-weaning. The CM0, CM7, and CM11 diets had lower (P < 0.05) coefficient of apparent total tract digestibility (CATTD) of DM, CP and GE than the SBM diet. Steam-explosion of canola meal reduced (P < 0.05) dietary CATTD of CP. For the 28-day nursery trial, average daily feed intake (ADFI) of pigs fed the CM0 diet was lower (P < 0.05) than of pigs fed the SBM diet. Steam-explosion of canola meal increased (P < 0.05) ADFI. Average daily gain (ADG) did not differ among pigs fed the 4 diets. Finally, gain-to-feed ratio (G:F) was greater (P < 0.05) for pigs fed the 3 canola meal diets than the SBM diet. In conclusion, steam-explosion reduced ileal digestibility of CP, most indispensable AA and GE of canola meal in grower pigs. Dietary inclusion of 200 g canola meal/kg supplemented with crystalline amino acids and canola oil to replace dehulled soybean meal reduced ADFI, but not ADG, and thus increased G:F in weaned pigs. Steam-explosion of canola meal reduced dietary CATTD of CP and increased ADFI, but not ADG or G:F of weaned pigs.

PSIV-A-10 Effects of Canola Meal Inclusion in Gestation and Lactation Diets with or without Probiotic on sow Performance, Milk Composition and Piglet Performance

Abstract The experiment was conducted to determine the effects of high dietary canola meal (CM) inclusion in gestation and lactation diets with or without probiotic supplementation on sow and litter performance and milk composition. Seventy-five sows were randomly allotted 1 of 3 diets to give 25 replicates per treatment. Diets consisted of a corn and soybean meal (SBM) control (CTRL) or the CTRL diet with SBM replaced by 300g/kg CM and fed with (CM+) or without (CM-) probiotic ActisafÒ Sc 47 supplementation. Sows BW and backfat thickness were determined on d 80 and 111 of gestation, d 1 post-farrowing, and at weaning on d 21. Milk samples were collected on d 1 and d 21 post-farrowing to determine milk composition. Piglets were weighed on d 0 and at weaning. Data were analyzed using the PROC MIXED procedure of SAS 9.4 for a randomized complete block design. Dietary treatment had no effect on sow performance, milk crude protein and lactose oligosaccharide composition, piglet ADG and weaning weight, and piglet survivability (P &amp;gt; 0.10). Sows fed CM- diet tended to fewer (P = 0.07) weaned piglets compared to sows fed CTRL diet, but no difference was found between sows fed the CM+ and CTRL diets (P &amp;gt; 0.10). At weaning, milk fat content for sows fed with CM+ diet was higher (P &amp;lt; 0.05) and tended to be higher (P = 0.09) compared with those fed the CTRL and the CM- diet, respectively. In conclusion, including 300g/kg CM in gestation and lactation sow diets supported similar sow and piglet performance as those the control diet, without affecting piglet survivability at weaning. Also, probiotic supplementation in CM diet increased sow milk fat content at weaning.

Heat processing increased the digestibility of phosphorus in soybean expeller, canola meal, and canola expeller fed to growing pigs.

It is hypothesized that heat processing may increase P digestibility in different protein sources fed to growing pigs. A study was conducted to determine the apparent total tract digestibility (ATTD) and standardized total tract digestibility (STTD) of P in soybean expeller (SBE) produced from oil extraction using dry extrusion and expelling and to investigate the effects of heat treatment on the ATTD and STTD of P in SBE, canola meal (CM), and canola expeller (CE) fed to growing pigs. Thirty-six growing barrows with an initial body weight of 19.0 ± 1.0 kg (mean ± SD) were assigned to 1 of 6 experimental diets in a completely randomized design to give 6 replicates per diet. The experimental design was a 3 × 2 factorial arrangement including three oilseed meals with or without heat treatment. The diets were formulated to contain non-autoclaved or autoclaved (at 121 °C for 60 min) SBE, CM, and CE as the sole source of P. Limestone was included in diets to maintain a Ca:total P ratio of 1.3:1 across diets. Pigs were individually housed in metabolism crates for 12 d, including 7 d for adaptation and 5 d for total collection of feces. Pigs were offered their daily ration at 2.8 times their maintenance energy requirement. Data were analyzed using the PROC MIXED of SAS. Heat treatment increased (P < 0.05) the ATTD and STTD of P. Pigs fed the SBE diets had greater (P < 0.05) ATTD and STTD of P than pigs fed CM and CE diets. For the autoclaved ingredients, the values of STTD of P were 49.4%, 23.2%, and 25.8% for SBE, CM, and CE, respectively, whereas STTD of P in non-autoclaved SBE, CM, and CE were 48.5%, 20.2%, and 22.5%. Heat treatment increased (P < 0.05) the ATTD of Ca. In conclusion, heat treatment increased ATTD and STTD of P and ATTD of Ca in SBE, CM, and CE fed to growing pigs. The ATTD and STTD of P in SBE determined in the current study were 41.0% and 48.5%, respectively.

Diet supplementation with canola meal improves milk production, reduces enteric methane emissions, and shifts nitrogen excretion from urine to feces in dairy cows

The objective of this study was to examine the effect of isonitrogenous substitution of solvent-extracted soybean meal (SBM) with solvent-extracted canola meal (CM) on enteric CH4 production, ruminal fermentation characteristics (including protozoa), digestion (in situ and apparent total-tract digestibility), N excretion, and milk production of dairy cows. For this purpose, 16 lactating Holstein cows, of which 12 were ruminally cannulated, were used in a replicated 4 × 4 Latin square (35-d periods; 14-d adaptation). The cows averaged (mean ± SD) 116 ± 23 d in milk, 692 ± 60 kg of body weight, and 47.5 ± 4.9 kg/d of milk production. The experimental treatments were control diet (no CM; 0%CM) and diets supplemented [dry matter (DM) basis] with 7.9% CM (8%CM), 15.8% CM (16%CM), or 23.7% CM (24%CM) on a DM basis. The forage:concentrate ratio was 52:48 (DM basis) and was similar among the experimental diets. Canola meal was included in the diet at the expense of SBM and soybean hulls, whereas the percentages of the other diet ingredients were the same. Intake of DM increased linearly, whereas apparent total-tract digestibility of DM, crude protein, neutral detergent fiber, and gross energy (GE) declined linearly as CM inclusion in the diet increased. Total volatile fatty acids concentration and butyrate molar proportion decreased linearly, whereas molar proportion of propionate increased linearly, and that of acetate was unaffected by CM inclusion in the diet. Ruminal ammonia concentration was not affected by inclusion of CM in the diet. Energy-corrected milk (ECM) yield increased linearly (up to 2.2 kg/d) with increasing CM percentage in the diet, whereas milk production efficiency averaged 1.63 kg of ECM/kg of DM intake and was unaffected by CM inclusion in the diet. Daily CH4 production decreased linearly with increasing CM percentage in the diet (489, 475, 463, and 461 g/d for 0%CM, 8%CM, 16%CM and 24%CM diets, respectively). As a consequence, CH4 emission intensity (g of CH4/kg of ECM) also declined linearly by up to 10% as the amount of CM increased in the diet. Methane production also decreased linearly when expressed relative to GE intake (5.7, 5.2, 5.1, and 4.9% for 0%CM, 8%CM, 16%CM and 24%CM diet, respectively). Quantity of manure N excretion was not affected by replacing SBM with CM; however, N excretion shifted from urine to feces as dietary percentage of CM increased, suggesting reduced potential for N volatilization. Results from this study show that replacing SBM with CM as a protein source in dairy cow diets reduced enteric CH4 emissions (g/d, % of GE intake, and adjusted for milk production) and increased milk production. The study indicates that CM can successfully, partially or fully, replace SBM in lactating dairy cow diets, with positive effects on animal productivity and the environment (i.e., less enteric CH4 emission and urinary N excreted). We conclude that compared with SBM, inclusion of CM meal in dairy cow diets can play a key role in reducing the environmental footprint of milk production.

Growth performance and gut health of Escherichia coli-challenged weaned pigs fed canola meal-containing diet.

An experiment was conducted to evaluate the effects of including canola meal (CM) in diets for weaning pigs challenged with a F18 strain of Escherichia coli on growth performance and gut health. A total of 36 individually housed weaned pigs (initial body weight [BW] = 6.22 kg) were randomly allotted to one of the three diets (12 pigs/diet). The three diets were corn–soybean meal (SBM)-based basal diet (control diet) and the basal diet with 0.3% zinc oxide, 0.2% chlortetracycline, and 0.2% tiamulin (antibiotic diet) or with 20% CM diet. The diets were fed in two phases: Phase 1: days 0 to 7 and Phase 2: days 7 to 20. All pigs were given an oral dose of 2 × 109 CFU of F18 strain of E. coli on day 7. Fecal score was assessed daily throughout the trial. Dietary antibiotics increased (P < 0.05) overall average daily gain (ADG) and average daily feed intake (ADFI) compared by 48% and 47%, respectively. Dietary CM increased (P < 0.05) overall ADG and ADFI by 22% and 23%, respectively; but the ADG and ADFI values for CM-containing diet did not reach those for the antibiotics-containing diet. Dietary antibiotics reduced (P < 0.05) fecal score; however, dietary CM unaffected fecal score. Dietary antibiotics decreased (P < 0.05) liver weight per unit live BW by 16% at day 20, whereas dietary CM did not affect liver weight per unit live BW (29.2 vs. 28.6). Also, dietary antibiotics increased (P < 0.05) serum triiodothyronine and tetraiodothyronine levels for day 14, whereas dietary CM did not affect the serum level of these hormones. Dietary antibiotics reduced (P < 0.05) the number white blood cells and neutrophils by 38% and 43% at day 20, respectively, whereas dietary CM tended to reduce (P = 0.09) the number white blood cells by 19% at day 20. The number white blood cells for CM diet tended to be greater (P < 0.10) than that for antibiotics diet. The dietary antibiotics decreased (P < 0.05) the concentration of individual volatile fatty acids and hence of total volatile fatty acid in cecum by 61% at day 20, whereas dietary CM decreased (P < 0.05) cecal butyric acid concentration by 61% and tended to reduce (P < 0.10) total volatile fatty acid concentration by 30% at day 20. In conclusion, the dietary inclusion of 20% CM improved ADG and tended to reduce white blood cell counts. Thus, inclusion of CM in antibiotics-free corn-SBM-based diets for weaned pigs that are challenged with F18 strain of E. coli can result in their improved performance partly through a reduction of the inflammatory response.

Partial and full substitution of fish meal and soybean meal by canola meal in diets for genetically improved farmed tilapia (O. niloticus): Growth performance, carcass composition, serum biochemistry, immune response, and intestine histology

ABSTRACT The study was designed to assess the effects of partial or full replacement of fish meal (FM) and soy bean meal (SBM) with canola meal (CM) on growth performance, health status, and cost benefit ratio of genetically improved farmed tilapia, Oreochromis niloticus. Fish (mean body weight 4.67 ± 0.07 g) were fed with five isonitrogenous (crude protein 396 g kg−1) diets: D I was set as the control; D II, D III, D IV, and D V were supplemented with CM at 25%, 50%, 75%, and 100% respectively. After 16 weeks of feeding, the optimum CM (%) for growth performance, feed utilization, carcass composition, and body indexes of O. niloticus were predicted at D III (P < 0.05). Also, the following linear regression equation was acquired for weight gain versus CM (%) in diets: Y = −2.542x + 23.232; R2 = 0.9474. The survival rate remained unaffected among all groups. Serum total protein, albumin content, and lysozyme activity increased in D I, D II, and D III; activity of liver functioning enzymes (SGPT and SGOT), triglycerides, total cholesterol, low-density lipoprotein-cholesterol, blood urea nitrogen, and creatinine level were elevated in groups D IV and D V (P < 0.05). Also, a significantly increased degree of variation and Uran’s pooled score were measured in intestine histology with increasing levels of CM supplementation (P < 0.05). Additionally, the optimum unitary feeding cost (UFC) was obtained in group D III (P < 0.05) along with polynomial equation: Y = 9.8 × 2– 47.39x + 176.44; R2 = 0.9674 between UFC and CM (%) in diets. Thus, FM and SBM could be replaced by CM up to 50% without compromising production, health status, and cost benefit for O. niloticus culture.

Performance and ileal amino acid digestibility in broilers fed diets containing solid-state fermented and enzyme-supplemented canola meals

Two experiments were conducted to determine the performance and ileal amino acid digestibility in growing male broilers fed diets containing solid-state fermented canola meal (SSFCM) and enzyme-supplemented canola meal (CM). In experiment 1 (performance trial), a total of 300-day-old Ross 308 male broiler chicks were allotted to six dietary treatments with five replicate pens of 10 birds each. Diet 1 was based on corn-soybean meal (SBM) as reference, diets 2 and 3 contained 300 g/kg CM and 300 g/kg SSFCM, respectively. Diet 4 contained 300 g/kg CM + phytase (PHY). Diet 5 contained 300 g/kg CM + xylanase (XYL). Diet 6 contained 300 g/kg CM + PHY + XYL. PHY at 500 FTU/kg and XYL at 1000 U/kg were added to the respective diets. During the experimental period (1–21 d), birds fed SBM and SSFCM diets recorded improved FI (p < 0.05). Broilers fed CM diet with enzyme supplementation had improved FCR compared to SSFCM diet. In experiment 2 (digestibility trial), a total of 240-twenty-one-day-old Ross 308 male broiler chicks were assigned to six dietary treatments with five replicate cages of eight birds each. Diet 1 was based on SBM, diets 2 and 3 contained 400 g/kg CM and 400 g/kg SSFCM, respectively. Diet 4 contained 400 g/kg CM + PHY. Diet 5 contained 400 g/kg CM + XYL. Diet 6 contained 400 g/kg CM + PHY + XYL. The inclusion of SSFCM and enzyme-supplemented CM in diets did not improve the apparent ileal digestibility (AID) of amino acids. The AID of calcium (Ca) was highest (p < 0.05) in SBM, CM and SSFCM. The AID of phosphorus (P) was increased (p < 0.05) in SBM and CM + PHY. Ca retention was higher (p < 0.05) in SSFCM and CM + PHY + XYL. Feeding SBM, SSFCM, CM + PHY and CM + PHY + XYL diets enhanced P retention. Further studies are required to establish the practical and nutritional implication of SSFCM on performance and ileal amino acid digestibility in broilers.

Response of meat chickens to different sources of arginine in low-protein diets.

Arginine activity in broiler diets can be supplied by L-arginine (Arg), guanidinoacetic acid (GAA) and L-citrulline (Cit), all of which are commercially available. This study was conducted to assess the effects of Arg source and level on broiler performance, nutrient digestibility and carcass parameters. Day-old Ross 308 cockerels (n=768) were assigned to one of eight dietary treatments using a completely randomized design: normal protein (NP), low protein deficient in Arg (LP) and LP with two levels of either Arg (0.238% and 0.476%), GAA (0.309% and 0.618%) or Cit (0.238 and 0.476%). The LP was 5 percentage points lower in protein level than the NP. Wheat, sorghum, soya bean meal, canola meal, and meat and bone meal-based diets were fed over three feeding phases to 6 replicate floor pens with 16 birds each. Compared to NP, birds fed LP had reduced feed intake (FI, p<0.001), reduced body weight gain (BWG, p<0.001) and increased feed conversion ratio (FCR, p<0.001) from day 0 to day 35. Additions of Arg or Cit to the LP at both levels resulted in increased BWG and reduced FCR (p<0.05). Birds fed LP with GAA added had lower FCR (p<0.05) but not higher BWG (p>0.05) compared with the LP observed from day 0 to day 35. Supplementation of Arg, Cit and the low level of GAA to LP resulted in increased carcass yield, bone length, diameter and ash (p<0.05) but did not increase ileal energy or nitrogen digestibility (p>0.05). The findings indicate that Cit is an efficacious source of Arg activity in Arg-deficient diets.

Swine diets impact manure characteristics and gas emissions: Part II protein source

Soybean meal is the dominate protein source for swine diets in the world driven largely by economics, nutritive value, and availability; but conditions can change requiring growers to consider more economical and available protein alternatives. A feeding trial was conducted to determine the impact dietary protein source material on manure slurry chemical properties and manure gas emissions. A total of 32 gilts averaging 130 kg BW were fed either a control diet formulated with soybean meal (SB) or an alternative protein source that included corn gluten meal (CG); canola meal (CM); or poultry meal (PM), with all diets containing 176 g protein kg−1. Diets were fed for 45 d with an average daily feed intake of 2.68 kg/d. Feces and urine were collected twice daily after each feeding and added to animal-specific manure storage containers. At the end of the study, manure slurries were monitored for gas emissions and chemical properties. Dietary protein source had a significant effect (P < 0.05) on manure pH, total solids, total C, protein N, and total S. Pigs fed the diets containing CM had significantly higher levels of sulfide, butanoic acid, and branch chain fatty acids compared to pigs fed SB diets (P < 0.05). Pigs fed CM diets had significantly lower emissions of NH3 compared to pigs fed SB diets (P < 0.05). There were no significant differences in C or S emissions or in odorant emission as affected by source of dietary protein. Hydrogen sulfide was the most dominate odorants for all dietary treatments.

PSVI-11 Non-GMO beet pulp and canola meal corn-soybean meal diet has comparable effects as that of GMO corn-soybean meal diet on the performance of sows and piglets

Abstract Consumers are concerned about the safety of livestock feed stuff with the increasing use of genetically modified organisms (GMO).The objective of the present experiment was to compare the effect of non-GMO sugar-beet pulp (SBP) and canola meal (CM) in the diet with GMO corn-soybean meal basal diets on reproduction performance in gestation-lactation sows and the performance of their litters. A total of 16 lactating sows (Landrace × Yorkshire, Parity = 2.4), were randomly allotted to 1 of 2 treatments with 8 sows per treatment starting from the last 4 weeks of gestation to 21 day of lactation. Treatments consisted of GMO diet (CON) and non-GMO diet (NO). Sows were individually fed gestation and lactation diets that were formulated to meet or exceed NRC (2012) recommendations for all nutrients. The amount of feed consumed during the lactation periods was recorded and used to calculate the average daily feed intake (ADFI). Number of piglets born and piglets weaned were also recorded to calculate the survival rate. Individual piglet body weight (BW) and litter weights were assessed on days 0, and 26 to calculate average daily gain (ADG). All data were subjected to the GLM procedures of SAS in a randomized complete block design. The variability in the data was expressed as standard error of means (SEM). A probability level of P&amp;lt; 0.05 was considered to be statistically significant. In the current study, NO treatment diets showed comparable effects on the BW, ADFI, backfat thickness, body condition score, and estrus interval of sows as that of basal diet. In addition, there was also no significant difference in growth performance of piglets fed NO treatment diets compared with CON diet (P &amp;gt; 0.05). In conclusion, the results of the current study indicated the utilization of non-GMO beet pulp, and canola meals do not have any adverse effect on the performance of sows and their litters.

Effect of including canola meal in diets of slaughter ostriches (Struthio camelus var. domesticus)

Canola meal (CM) is a locally produced protein source that may be less expensive than soybean meal (SBM). This study evaluated the effects of replacing 0%, 25%, 50%, 75%, and 100% SBM with CM in diets for slaughter ostriches. The CM was added at the expense of SBM and other concentrates, with minor changes in other ingredients. Birds (n = 15 per treatment) were reared from 77 to 337 days old on the trial diets, which were supplied ad libitum for starter, grower, and finisher phases. Bodyweights and feed intake were measured during these phases. No differences (P &gt;0.05) were found between treatments for live weight at the end of each phase, dry matter intake (DMI), average daily gain (ADG) and feed conversion ratio (FCR) over all the growth phases. Although no differences were observed in live weight at the end of each phase, the birds reared on the diet with 50% CM were heaviest at slaughter, and birds reared with 100% CM were lightest (P &lt;0.05). Differences (P &lt;0.05) between diets were observed for the weight at slaughter, weights of the liver and thyroid glands and the pH of the cold carcass. However, no differences (P &gt;0.05) were observed between diets for fat pad weight, dressing percentage, and weights of thighs and Muscularis gastrocnemius. The results indicate that CM could replace SBM in the diets of slaughter ostriches without affecting production traits and slaughter yields.Keywords: alternative protein, average daily gain, canola, dry matter intake, feed conversion ratio, growth, ostrich nutrition, production

Nutritional evaluation of canola meal as fish meal replacement for juvenile spotted rose snapper (Lutjanus guttatus): Effects on growth performance, hematological parameters, body composition, and nutrient digestibility

This study examined the nutritional value of solvent-extracted canola meal (CM) as a replacement for fish meal (FM) in practical diets for spotted rose snapper (Lutjanus guttatus). Four isonitrogenous (490 g/kg crude protein), isoenergetic (19.03 kJ/g) diets containing 0 g/kg (CM0), 150 g/kg (CM150), 300 g/kg (CM300), or 450 g/kg (CM450) of CM protein instead of FM protein were used. All CM diets were supplemented with phytase and protease enzymes; dl-methionine was supplemented to the CM450 diet. Each diet was fed to triplicate groups of 15 fish (average body weight 36.2 ± 0.03 g) per tank to apparent satiation three times a day for 70 days. A one-way ANOVA showed that the specific growth rate (SGR) was significantly lower when CM inclusion was 26.67 % or higher, replacing 450 g/kg of fish meal protein or more. There was a significant negative relationship between growth response and the level of FM protein replacement. Survival and dry feed intake (DFI) were unaffected by fish meal replacement. The protein efficiency ratio (PER) was highest at the lowest CM inclusion level. Hematological parameters, including hematocrit, protein, hemoglobin, triglycerides, and cholesterol, did not vary between treatments. Juvenile fish fed the 450 g/kg CM diet showed the lowest protein content. The apparent digestibility coefficient (ADC) of dry matter and energy were significantly reduced by CM addition.Our results show that CM can be a suitable alternative protein source for spotted rose snapper juveniles heavier than 37 g. Under the experimental conditions tested in this study, up to 300 g/kg FM protein can be replaced by CM protein with no significant adverse effects on growth, feed utilization, hematological parameters, or body composition in this species.

Effects of replacing canola meal with camelina expeller on intake, total tract digestibility, and feeding behavior of beef heifers fed high-concentrate diets.

To assess the effect of inclusion of camelina expeller in beef cattle diets, 24 Simmental heifers were used. Two experiments were carried out. In the first, two free-choice tests, one without and another with molasses, were conducted to know the preference of animals for a total mixed ration (TMR) made with a 90 to 10 concentrate to barley straw ratio, where canola meal (CM) or camelina expeller (CE) was used in the concentrate as a protein source. Heifers were allotted in four pens with two independent feedbunks, one for each diet. In the second, a replicated 4 × 4 Latin square design was used to assess the effects of the replacement of CM with CE on intake, digestibility, and sorting and animal behavior. The experiment was performed in four 28-d periods during which groups of three animals were allotted in each pen of 12.5 m2. Diets were formulated with a 90 to 10 concentrate to barley straw ratio and fed as TMR, and they were designed to contain 1) CM as main protein source and 0% of CE (0CE), 2) 3% of CE replacing CM (3CE), 3) 6% of CE replacing CM (6CE), and 4) 9% of CE replacing CM (9CE). In the free-choice test without molasses, heifers showed a greater preference for CM than for CE (38.6 vs. 8.7 kg/d; P < 0.001). When molasses were added to the diet, the preference for CM was maintained (39.1 vs. 9.8 kg/d; P < 0.001). Dry matter (DM), organic matter (OM), crude protein (CP), and neutral detergent fiber (NDF) intake was unaffected by the level of replacement of CM by CE (P > 0.10), and there was no effect of this replacement on DM, OM, CP, and NDF apparent digestibility (P > 0.10). Intake of long particle size increased lineally as CE proportion increased (P = 0.015). In addition, extension of sorting behavior for long particle size tended to increase lineally (P = 0.07), and sorting against this particle size was detected in 0CE and 3CE, but not in 6CE and 9CE (P < 0.05). However, the results recorded for long particle size intake and for sorting behavior against these particles did not translate into more time spent ruminating in heifers fed diets with higher proportion of CE. In conclusion, when canola meal was replaced with camelina expeller at more than 14% of inclusion, heifers preferred the canola meal diet. However, replacing canola meal with camelina expeller up to 9% of inclusion in diets for beef cattle did not affect intake and digestibility but promoted a greater intake of long particle size of barley straw.

Research Note: Phosphorus digestibility in conventional canola meal determined using different balance assays

Three experiments were conducted to determine ileal P digestibility and excreta P retention values for canola meal (CM) using 3 different types of balance assays. The first experiment was an ad libitum–fed chick experiment which evaluated the effect of phytase on ileal P digestibility and excreta P retention values. Chicks were fed a P-deficient cornstarch–dextrose-45% CM basal diet (0.13% nonphytate P) as diet 1 or that diet plus 125 or 250 FTU/kg of phytase, respectively, from 8 to 21 D of age. The digestibility/retention of P was 38% and phytase linearly increased both ileal digestibility and excreta retention of P (P < 0.05). The second experiment was a precision-fed chick assay conducted to determine ileal digestibility of P in CM at 21 D. Mean ileal P digestibility was determined to be 47.5% in chicks fed 6 g and 40.0% in chicks fed 9 g of CM and the values were not significantly different. Experiment 3 was an ad libitum–fed chick assay to determine ileal P digestibility and excreta P retention for CM with and without increasing levels of dietary supplemental Ca. The chicks were fed P-deficient - dextrose - CM diets containing increasing levels of 13.5, 27, 40.5, or 54% CM, respectively, with Ca:nonphytate P ratio maintained at 2:1 in diets 1–4 and 6:1 in diets 5–8. Based on regression analysis of ileal digesta or excreta P output on dietary P concentration, digestibility/retention of P in CM was 30%. Ileal P digestibility (and to a lesser extent excreta P retention) at 21 D was reduced by increased Ca:P ratio. The results of this study indicated that the 3 balance assays yielded reasonably consistent values of 30–40% for P digestibility/retention and ileal P digestibility was greatly affected by Ca:P ratio.

Replacement of fishmeal with processed canola meal in diets for juvenile Nile tilapia (Oreochromis niloticus): Growth performance, mucosal innate immunity, hepatic oxidative status, liver and intestine histology

The present study was carried out to evaluate the effect of processed canola meal (PCM) as fishmeal (FM) substitute in juvenile Nile tilapia diet. Firstly, canola meal (CM) was dephytinized using 2000 IU/kg phytase and subjected to methanolic ammonia solution (MAS). Finally, five isonitrogenous and isoenergetic diets were formulated by inclusion of 0 (control), 12.5, 25, 37.5 and 50% PCM at the expense of dietary FM. Each experimental diet was fed to three randomly allotted groups of 17 juvenile Nile tilapia with an average body weight of 3.5 ± 0.1 g for 36 days. Fish were feed three times a day until apparent satiation. Results showed that there were no statistically significant differences regarding SGR, final body weight and WG between control group and PCM2 (i.e., diet with 25% PCM inclusion level) (p > .05); however receiving diets with higher PCM content (>25%) resulted in significantly decreased growth indices (p < .05). Meanwhile, FCR, FI and apparent digestibility coefficient of protein (ADCprotein) did not differ among experimental groups (p > .05). Digestive enzyme activities, mucosal innate immunity, liver antioxidant enzyme activities and liver tissue malondialdehyde content (MDA) were not significantly affected by dietary inclusion of PCM (p > .05). However, relative gene expression of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were significantly increased by dietary PCM content increment (p < .05). In line with growth performance, histological observations revealed that those fish received higher levels of dietary PCM content (i.e. 37.5 and 50%) showed intensive intestinal and hepatic mononuclear immune cell infiltration, lamina propria expansion and intestinal villus detachment and shortening (p < .05). In conclusion, despite containing lower glucosinolate, phytic acid, phenolic compounds and tannins well within the recommended thresholds for aquatic animals nutrition, diets containing PCM beyond 25% negatively affected the growth performance and intestine and liver tissue histoarchitecture of juvenile Nile tilapia. However, further complementary studies are needed regarding the effects observed on gut histology and expression of antioxidant enzyme genes.

Performance of dairy cows fed diets formulated at 2 starch concentrations with either canola meal or soybean meal as the protein supplement

This study was designed to evaluate the effects of substituting corn grain with nonforage fiber sources in diets containing soybean meal (SBM) or canola meal (CM) as the primary protein source. Sixteen Holstein cows were assigned to a replicated 4 × 4 Latin square design with 4 periods of 28 d each. Treatments were arranged as a 2 × 2 factorial with 2 protein sources (SBM and CM) and 2 dietary starch concentrations (21 and 27% dry matter, DM). Diets were formulated to contain 16.5% CP, and the 21% starch diets were obtained by replacing corn grain with soybean hulls and beet pulp. Protein source × starch interactions were observed for DM intake (DMI), milk fat and protein concentrations, milk protein yield, milk urea nitrogen, and feed efficiency. Cows fed CM diets had a higher DMI when dietary starch concentration was 27% compared with 21%, but those cows had DMI similar to that of cows on SBM diets regardless of the starch concentration. Milk fat percentage was decreased in cows fed CM with 27% starch compared with cows fed CM with 21% starch and cows fed SBM with 27% starch. Milk protein percentage and yield and milk lactose percentage were least in cows fed CM with 21% starch compared with the other 3 diets, but feed efficiency was greater for cows fed CM with 21% starch. Milk urea nitrogen was least in cows fed CM with 27% starch compared with the other 3 diets. Cows fed diets with 27% starch produced 2.5 kg/d more milk and 1.9 kg/d more energy-corrected milk compared with cows fed 21% starch. Digestibility of DM and organic matter was higher in cows fed SBM diets than in cows on CM diets, and cows fed 27% starch showed greater DM and organic matter digestibility than cows on 21% starch. Digestibility of neutral detergent fiber and acid detergent fiber was greater in diets with SBM than in those with CM. Molar proportion of acetate was the lowest for cows fed CM with 21% starch compared with cows fed SBM with 21% starch, with the remaining cows fed being intermediate and similar. However, propionate was highest for cows fed CM with 21% starch than for cows fed SBM with 21% starch, but the remaining treatments were intermediate and similar. Isobutyrate was greater for cows fed CM with 21% starch, which resulted in the lowest acetate:propionate ratio compared with cows fed the remaining treatments. Overall, we confirmed that the interaction of protein with starch in CM diets can sustain similar cow performance as with the SBM diets. Those making decisions about starch concentration and protein source should consider feed price when SBM or CM and different starch levels are being formulated in diets for lactating dairy cows.