Canavalia Gladiata Research Articles

Bio-reinforcement efficiency of enzyme-induced carbonate precipitation modified by sword bean crude urease combined with multiple enhancers on bio-cemented sand

Bio-reinforcement efficiency of enzyme-induced carbonate precipitation modified by sword bean crude urease combined with multiple enhancers on bio-cemented sand

Forage Quality of Sword Bean (Canavalia gladiata) Leaves as Influenced by Harvest Stage

Forage Quality of Sword Bean (Canavalia gladiata) Leaves as Influenced by Harvest Stage

An Enzyme-Induced Carbonate Precipitation Method for Zn2+, Ni2+, and Cr(VI) Remediation: An Experimental and Simulation Study

Heavy metal contamination has long been a tough challenge. Recently, enzyme-induced carbonate precipitation (EICP) has been proposed to handle this problem. This paper aims to explore the efficacy, process, and mechanisms of EICP using crude sword bean urease extracts to remediate Zn2+, Ni2+, and Cr(VI) contamination. A series of liquid batch tests and geochemical simulations, as well as microscopic analyses, were conducted. The liquid batch test results show that Zn2+, Ni2+, and Cr(VI) can be effectively immobilized by the EICP method, and the highest immobilization percentage was observed for Zn2+, reaching up to 99%. Ni2+ and Cr(VI) were immobilized at 62.4% and 24.4%, respectively. Additionally, the immobilization percentage of heavy metals increased with the concentration of added Ca2+. The simulation results and XRD results reveal that the organic molecules in crude sword bean urease can promote ZnCO3, Zn(OH)2, Zn5(CO3)2(OH)6, and NiCO3 precipitation. The FTIR and SEM-EDS results provide evidence for heavy metal adsorption by the functional groups in crude urease and calcium carbonate. The liquid batch test results, as well as the simulation results and the microscopic analysis results, indicate that the mechanism of EICP in heavy metal remediation can be summarized as biomineralization to form heavy metal carbonate precipitates and metal hydroxide precipitates, adsorption by calcium carbonate, and adsorption or complexation or promoting nucleation by organic molecules.

Effects of enzyme-induced carbonate precipitation technique on multiple heavy metals immobilization and unconfined compressive strength improvement of contaminated sand

Enzyme-induced carbonate precipitation (EICP) has been studied in remediation of heavy metal contaminated water or soil in recent years. This paper aims to investigate the immobilization mechanism of Zn2+, Ni2+, and Cr(VI) in contaminated sand, as well as strength enhancement of sand specimens by using EICP method with crude sword bean urease extracts. A series of liquid batch tests and artificially contaminated sand remediation experiments were conducted to explore the heavy metal immobilization efficacy and mechanisms. Results showed that the urea hydrolysis completion efficiency decreased as the Ca2+ concentration increased and the heavy metal immobilization percentage increased with the concentration of Ca2+ and treatment cycles in contaminated sand. After four treatment cycles with 0.5 mol/L Ca2+ added, the immobilization percentage of Zn2+, Ni2+, and Cr(VI) were 99.99 %, 86.38 %, and 75.18 %, respectively. The microscale analysis results presented that carbonate precipitates and metallic oxide such as CaCO3, ZnCO3, NiCO3, Zn(OH)2, and CrO(OH) were generated in liquid batch tests and sand remediation experiments. The SEM-EDS and FTIR results also showed that organic molecules and CaCO3 may adsorb or complex heavy metal ions. Thus, the immobilization mechanism of EICP method with crude sword bean urease can be considered as biomineralization, as well as adsorption and complexation by organic matter and calcium carbonate. The unconfined compressive strength of EICP-treated contaminated sand specimens demonstrated a positive correlation with the increased generation of carbonate precipitates, being up to 306 kPa after four treatment cycles with shear failure mode. Crude sword bean urease with 0.5 mol/L Ca2+ added is recommended to immobilize multiple heavy metal ions and enhance soil strength.

Mangrove Sword Bean Food Bar as the Emergency Food Product for Children Under the Age of Five Due to Landslides

Mangrove Sword Bean Food Bar as the Emergency Food Product for Children Under the Age of Five Due to Landslides

Optimization and characterization of immobilized thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds on DEAE-cellulose and chitosan bead for operational stability.

Thermostable α-amylase from germinating Sword bean (Canavalia gladiata (Jacq.) DC.) seeds has been successfully immobilized on DEAE-cellulose (ICgAmy1) and chitosan bead (ICgAmy2) support materials. Optimum conditions of immobilization for DEAE-cellulose and chitosan bead revealed 97% and 96% immobilization yield, respectively. The optimum pH and temperature of both DEAE-cellulose and chitosan bead immobilized α-amylases were pH 7 and 70°C. Both ICgAmy1 and ICgAmy2 were high stability over a wide pH range of pH 5-9 and a temperature range of 70-90°C. In addition, ICgAmy1 and ICgAmy2 led to an operationally stable biocatalyst with above 74% and 76% residual activity after 10 reuses, respectively. Immobilized α-amylases showed high storage stability with 81% (ICgAmy1) and 85% (ICgAmy2) residual activity after 120 days of storage. The easy immobilization process on low-cost, biodegradable, and renewable support materials exhibited an increase in the enzyme operation range and storage stability which reduces production costs. This makes immobilized amylases an effective biocatalyst in various industrial applications especially a potential candidate for bioethanol production, a key renewable energy source.

Identification of Interleukin (IL)-33 Inhibitory Constituents from Canavalia gladiata Pods.

Interleukin (IL)-33, a member of the IL-1 cytokine family, plays a vital role in immune system regulation and inflammation, with oxidative stress being implicated in its expression. During the search for compounds from natural sources with potential as therapeutic agents for allergic diseases via IL-33 signal modulation, we discovered significant IL-33 inhibitory activity in the methanol extract of Canavalia gladiata (sword bean) pods. Through chromatographic separation and liquid chromatography-mass spectrometry, we isolated 11 compounds (1-11) from the methanol extract. Furthermore, we assessed the inhibitory effects of these substances on IL-33/ST2 signaling in processes related to inflammatory and autoimmune diseases using an enzyme-linked immunosorbent assay. Among them, compounds 7, 10, and 11 exhibited substantial IL-33 inhibitory efficacy, with values reaching 78%, 86%, and 79% at 100 µM, respectively. Remarkably, compounds 7, 10, and 11 demonstrated significant and dose-dependent inhibition of IL-33 signaling at concentrations of 10, 50, and 100 µM. Computational molecular docking and dynamic simulations further elucidated the underlying mechanisms. These findings have promising pharmacological implications for allergy prevention and treatment associated with flavonoid glycosides derived from C. gladiata.

Preliminary Phytochemical Screening and Ethno-medical Importance of Sword Bean

Canavalia gladiata (jacq)DC is a lesser-known bean commonly called sword bean. This study deals with the preliminary phytochemical screening and ethno-medicinal properties of sword beans. The phytochemical assessments of the sword bean seed show the medicinal value of the plant. Alkaloids, Tannins, terpenoids, flavonoids, Cardiac glycosides, Reducing sugars, Proteins, and Carbohydrates showed positive results in three types of solvents viz., water, Methanol, and Acetone solvents. The whole 1Q2plant of sword bean used in many traditional and ethano-medicinal practices by their applications.

Recent Insights into Glucose-Responsive Concanavalin A-Based Smart Hydrogels for Controlled Insulin Delivery.

Many efforts are continuously undertaken to develop glucose-sensitive biomaterials able of controlling glucose levels in the body and self-regulating insulin delivery. Hydrogels that swell or shrink as a function of the environmental free glucose content are suitable systems for monitoring blood glucose, delivering insulin doses adapted to the glucose concentration. In this context, the development of sensors based on reversible binding to glucose molecules represents a continuous challenge. Concanavalin A (Con A) is a bioactive protein isolated from sword bean plants (Canavalia ensiformis) and contains four sugar-binding sites. The high affinity for reversibly and specifically binding glucose and mannose makes Con A as a suitable natural receptor for the development of smart glucose-responsive materials. During the last few years, Con A was used to develop smart materials, such as hydrogels, microgels, nanoparticles and films, for producing glucose biosensors or drug delivery devices. This review is focused on Con A-based materials suitable in the diagnosis and therapeutics of diabetes. A brief outlook on glucose-derived theranostics of cancer is also presented.

Effectiveness of mangrove sword bean food bar addressed to older people of landslide disaster victims.

Older people require extra attention due to their reduced ability to prepare for disasters, as they adequately possess distinct needs. These groups necessitate uncomplicated, readily consumable, and palatable food options that fulfill their micronutrient needs. The objective of this research was to assess the effects of a snack bar enriched with api-api mangrove (Avicennia marina) and sword bean (Canavalia ensiformis) on the body weight and Body Mass Index (BMI) of older people individuals afflicted by a landslide event. A non-randomized pre-post-intervention study was undertaken, involving 31 senior participants. The intervention group consisted of 15 seniors who were provided with a mangrove sword bean snack bar, while the control group comprised 16 seniors who received a sword bean food bar during 15 days. All study participants received education on maintaining a balanced diet for older people individuals. The data analysis involved using univariate and bivariate analyses, explicitly applying the independent t-test and dependent t-test. In the hedonic evaluation, the mangrove sword bean food bar had superior average attributes in terms of scent, flavor, texture, and color compared to the sword bean food bar. The consumption of snack bars made from mangrove sword beans resulted in a significant rise in weight (0.2 kg), energy intake (240.8 kcal), protein content (5.8 g), carbohydrate content (40.06 g), and fat content (4.4 g). Carbohydrate can significantly increase weight in the treatment subjects. Furthermore, the provision of comprehensive nutrition education has the potential to enhance the post-study knowledge score, as seen by the observed increase of 40.6. A significant disparity was observed between the mean carbohydrate consumption and understanding of balanced nutrition among the intervention and control groups. Api-api mangrove sword bean snack bars have been identified as a viable and efficient substitute for emergency food provisions, particularly in disaster-stricken communities. These food bars have demonstrated a significant capacity to contribute to the weight gain of individuals within such groups, thus addressing the nutritional needs of impacted populations in the aftermath of natural calamities. Subsequent investigations may include employing pregnant women as participants to explore the issue above. Clinicaltrials.gov, identifier: NCT05897892.

Insights into the Corrosion Inhibition Mechanism of Canavalia gladiata Leaf Extract for Copper in Sulfuric Acid Medium.

Canavalia gladiata leaf extract (CGLE) is extracted from crop waste employing a water decoction method. By employing electrochemical techniques, morphology analysis, quantum chemical calculations, and other methods, we extensively investigated the anticorrosion efficacy of CGLE on copper within a H2SO4 solution. The outcomes reveal that at 298 K, a CGLE concentration of 800 mg/L attains a remarkable inhibition efficiency (IE) of 96.8%. Additionally, we examined the impact of CGLE on the corrosion resistance of copper at varying temperatures. Even with rising temperatures, CGLE manages to sustain an IE of over 95%. This indicates that CGLE is mainly chemisorption based on the copper, leading to a strong adsorption. The surface test results show a noteworthy decrease in the extent of copper surface corrosion upon the introduction of CGLE. The study of the adsorption model demonstrates the alignment of CGLE adsorption onto the copper with the Langmuir adsorption.

The genomes of 5 underutilized Papilionoideae crops provide insights into root nodulation and disease resistance.

The Papilionoideae subfamily contains a large amount of underutilized legume crops, which are important for food security and human sustainability. However, the lack of genomic resources has hindered the breeding and utilization of these crops. Here, we present chromosome-level reference genomes for 5 underutilized diploid Papilionoideae crops: sword bean (Canavalia gladiata), scarlet runner bean (Phaseolus coccineus), winged bean (Psophocarpus tetragonolobus), smooth rattlebox (Crotalaria pallida), and butterfly pea (Clitoria ternatea), with assembled genome sizes of 0.62 Gb, 0.59 Gb, 0.71 Gb, 1.22 Gb, and 1.72 Gb, respectively. We found that the long period of higher long terminal repeat retrotransposon activity is the major reason that the genome size of smooth rattlebox and butterfly pea is enlarged. Additionally, there have been no recent whole-genome duplication (WGD) events in these 5 species except for the shared papilionoid-specific WGD event (∼55 million years ago). Then, we identified 5,328 and 10,434 species-specific genes between scarlet runner bean and common bean, respectively, which may be responsible for their phenotypic and functional differences and species-specific functions. Furthermore, we identified the key genes involved in root-nodule symbiosis (RNS) in all 5 species and found that the NIN gene was duplicated in the early Papilionoideae ancestor, followed by the loss of 1 gene copy in smooth rattlebox and butterfly pea lineages. Last, we identified the resistance (R) genes for plant defenses in these 5 species and characterized their evolutionary history. In summary, this study provides chromosome-scale reference genomes for 3 grain and vegetable beans (sword bean, scarlet runner bean, winged bean), along with genomes for a green manure crop (smooth rattlebox) and a food dyeing crop (butterfly pea). These genomes are crucial for studying phylogenetic history, unraveling nitrogen-fixing RNS evolution, and advancing plant defense research.

Chemical structure and gelation characteristics of a purified gel derived from sword beans (Canavaliagladiata)

Herein, a new method was developed to obtain a crude extract from sword beans at a higher extraction efficiency. The crude extract formed a gel at 8 °C, which melted at 70 °C, and lyophilization of the purified gel produced a powder that could be dissolved in distilled water at a concentration of 7 % (w/w) or less. A 3 % powder solution gelled at 12 °C and melted at 60 °C. The infrared spectrum of the gel powder was consistent with that of starch. Furthermore, a 4-aminobenzoic acid ethyl ester-labeling analysis revealed that glucose was the constituent sugar in the powder, and the powder solution reacted strongly in a starch–iodine test. These observations confirmed that the gelling substance was starch. However, the melting and gelling temperatures were dissimilar to those of other starches frequently used in the food industry. Thus, our results provide valuable information for using sword bean starch as a novel food material.

Complete Remission of Multiple Pulmonary Metastases of Endometrial Adenocarcinoma Treated with a Mixture of Low Molecular Weight Fucoidan and Canvaria gladiata Extract: A Case Report

Endometrial cancer (EC) is the most common malignant tumor in post-menopausal women, and the lung is one of the organs to which endometrial adenocarcinoma metastasizes. Chemotherapy is helpful for various malignancies, but not all are successful. A 71-year-old-Japanese-woman was diagnosed with metastatic lung cancer stage IV of primary endometrial adenocarcinoma. She received chemotherapy and hormone therapy after a total hysterectomy and pelvic lymphadenectomy. Subsequently, she visited this clinic for a second opinion as the metastatic lung cancer had repeated remissions and recurrences. Here, the authors report a case in which complete remission of metastatic lung cancer was achieved by using a product containing low molecular weight fucoidan extract (LMF) and Canavalia gladiata extract (CG) (LMF-CG) in combination with Medroxyprogesterone acetate (MPA) hormone therapy.

Morphological Characterization of Underutilized Legume Sword Bean (Canavalia gladiata Jacq.) Genotypes of North-Eastern Hill Region of India Under Foot Hill of Arunachal Pradesh

Morphological Characterization of Underutilized Legume Sword Bean (Canavalia gladiata Jacq.) Genotypes of North-Eastern Hill Region of India Under Foot Hill of Arunachal Pradesh

작두콩(Canavalia ensiformis) 발효액이 항산화, 항염증 및 항균 활성에 미치는 영향

In the present study, various experiments were performed to evaluate the biological activities, such as the antioxidant, anti-inflammation, and antimicrobial activities of sword bean (Canavalia ensiformis) fermentation filtrate by Lactobacillus plantarum (L. plantarum) and Lactobacillus brevis (L. brevis). Total polyphenol (TPC) and flavonoid contents (TFC) of sword bean were significantly decreased after fermentation regardless of Lactobacillus sp. (p<0.05). The DPPH radical scavenging activity of sword beans also decreased after fermentation. However, nitric oxide (NO) radical scavenging activity conspicuous increased after fermentation (p<0.001) in a treated concentration-dependent manner, and the effect for L. brevis was higher than for L. plantarum. In addition, the sword bean fermentation filtrate showed a strong inhibitory effect against Pseudomonas aeruginosa, Staphylococcus sp., and Escherichia coli. Cell cytotoxicity was not exhibited in all experimental groups (data not shown). These findings suggest that the sword bean fermentation filtrate may be used effectively in various industries due to its high anti-inflammatory and antimicrobial activities.

Sword Bean (Canavalia gladiata) Pods Induce Differentiation in MC3T3-E1 Osteoblast Cells by Activating the BMP2/SMAD/RUNX2 Pathway.

Sword bean (SB) contains various phytochemicals, such as flavonoids, tannins, saponins, and terpenoids. Although the evaluation of its potential functions, including antioxidant, anti-obesity, anti-inflammatory, liver protection, and antiangiogenic activities, has been widely reported, research on their use in osteoporosis prevention is insufficient. Furthermore, while various studies are conducted on SB, research on sword bean pods (SBP) is not yet active, and little is known about it. Therefore, this study investigated the effects of promoting osteoblast differentiation of MC3T3-E1 cells using SB and SBP extracts and their mechanisms. We show that SBP extracts increase osteoblast proliferation, mineralization-activated alkaline phosphatase (ALP), and collagen synthesis activities. Additionally, treatment with SBP extract increased the expression of markers related to osteoblast differentiation, such as ALP, SPARC, RUNX2, COL-I, BMP2, OCN, and OPN. It was confirmed that SBP induces differentiation by activating the BMP2/SMAD/RUNX2 pathway. We also show that SBP is more effective than SB, and SBP may be useful in assimilating bone minerals and preventing osteoporosis.

Gamma-Ray Radiation Effect on Two Species of Canavalia Performance

In Indonesia, sword beans are still only occasionally grown since the superior variety is not readily available. An alternative breeding technique to enhance plant characteristics is mutation. The goal of this study was to determine how gamma radiation affected the growth and performance of the two Canavalia species. Four accessions of two Canavalia species each received one of five gamma-ray treatments (0, 50, 100, 150, and 200 Gy). Plant morphology during the germination phase, growth type, plant capacity to produce flowers and pods, flowering age, maturity age, seed weight per plant, seed weight per plot, and seed weight per hundred seeds were all observational factors. Using a factorial randomized complete block design, the data were examined. Microsoft Excel was used for the descriptive analysis, whereas STAR software was used for the analysis of variance and further analyses. Gamma-ray doses of up to 200 Gy have not been successful in altering the characteristics or reaching the LD50 in sword bean. The weight of the seeds per plant and per hundred seeds varied significantly, but the dose and accession variables were inconsistent, making it impossible to determine the ideal combination of the two parameters at this time.

Physicochemical and functional properties of seed flours obtained from germinated and non-germinated Canavalia gladiata and Mucuna pruriens

C. gladiata and M. pruriens are underutilized legumes available in Sri Lanka. The physicochemical and functional properties of germinated and non-germinated flours from these two legumes were investigated. Protein contents of the flours of C. gladiata and M. pruriens increased by 17.04% and 14.69% respectively while fat and carbohydrate contents decreased due to germination. Higher Fe, Cu, Zn, Mn, and Se content was observed in both germinated flour types. Glutamic acid is the highest non-essential amino acid whereas leucine is the highest essential amino acid found in both flour types. The majority of amino acids in 100 g of seed flour of both legumes increased due to germination. Moreover, some of the functional properties of legume flours were changed such as swelling power, water and oil holding capacities because of the germination. In conclusion, the functional and nutritional properties of flour can be altered by the germination process making them ideal for utilization as ingredients for functional food formulations.

Sword Bean (Canavalia gladiata): a new host of Bean common mosaic virus.

Sword bean (Canavalia gladiata) is an underutilized legume that has the potential to become an important food source owing to its wide range of nutritional and medicinal properties. In May 2023, symptoms induced by a possible virus infection such as mosaic, mottling and vein banding were observed on the leaves of about 20% of the Sword bean plants growing at the experimental research farm of the Indian Agricultural Research Institute in Pune, Maharashtra, India. Symptomatic and asymptomatic samples were screened by ELISA for the presence of Potyvirus, Cucumber mosaic virus and Tobacco mosaic virus. All symptomatic samples tested positive for Potyvirus in ELISA as well as in RT-PCR assay using the universal potyvirus primer pair (CPUP /P9502) which amplify c. 700bp of the partial coat protein region and 3'UTR. Asymptomatic samples tested negative for all tested viruses in both serological and molecular assays. BLASTn sequence analysis of the amplicons revealed that the sequence shares more than 98% identity with an Indian isolate of Bean common mosaic virus (BCMV). Sequence analysis enabled the identification of the Potyvirus as BCMV. Furthermore, the present Sword bean isolate clustered with other BCMV isolates in the phylogenetic analysis. In the present study, BCMV was found to be naturally infecting Sword bean for the first time in the world. This is of epidemiological importance, as BCMV is known to cause significant yield losses in legumes and could severely hamper Sword bean production.