Tea Camellia Sinensis Research Articles

Emerging contaminant (Triclosan) removal by adsorption and oxidation process: comparative study

Triclosan is an antibacterial and antifungal agent present in a wide range of personal care products, including toothpaste, soaps, detergents, and surgical and surface cleaning agents. Many studies have been conducted to investigate the toxic potential of Triclosan (TCS) on human health, plants, and the environment, and it has been reported to be an endocrine disruptor. This study addresses the removal efficiency of TCS using nano-zerovalent iron (nZVI), multi-walled carbon nanotubes (MWCNTs), and carbon filter. Wherein, nZVI is synthesized by an environmentally benign method, i.e., using Camellia Sinensis (Tea) extract used as a reducing agent and carbon filter is prepared by coating MWCNTs onto the cotton cloth. Removal efficiency has been studied for TCS at different operational parameters such as concentrations of TCS from (5 to 20 ppm), dosages of nZVI and dosage of MWCNTs (0.05–0.3 g/l) and pH (3–11). Results of this study indicate that, till a concentration of 5 ppm, 0.1 g/lit dosage of nZVI and MWCNTs has shown a removal efficiency of 97.07 and 100% at reaction time of 40 and 20 min, respectively. An incorporation carbon filter has been proven an efficient than dispersing MWCNTs in aqueous solutions.

Effect of Drying and Steeping Temperatures on the Phenolic Content, Antioxidant Activity, Aromatic Compounds and Sensory Properties of Cunila polyantha Benth. Infusions

Cunila polyantha Benth. (Lamiaceae), an aromatic plant endemic to Mexico, is used in traditional medicine as tea infusions. In this study, the effects of different drying and steeping temperatures on the phenolic content and composition, antioxidant activity, volatile composition, and sensory properties of C. polyantha infusions were determined. Commercial green tea (Camellia sinensis L. Kuntze) was used as a control. The phenolic compounds identified in the C. polyantha infusions by high-performance liquid chromatography (HPLC) include phenolic acids such as gallic acid, chlorogenic acid, caffeic acid, and p-coumaric acid, flavonoids such as epigallocatechin gallate, protocatechin, quercetin, and naringenin, as well as the phenolic aldehyde vanillin. The C. polyantha infusions showed scavenging activity of DPPH• and ABTS•+ radicals as well as relevant antioxidant capacity, which was dependent on tea preparation conditions. A total of 46 volatile organic compounds (VOCs) were detected from the leaves and flowers of C. polyantha, while in the control group, a total of 30 VOCs were identified. Differences in consumer acceptability of C. polyantha infusions prepared at different conditions were observed. This research highlights the importance of linking sensory and chemical data to obtain the best sensorial quality and the optimal nutraceutical properties in C. polyantha infusions.

The Free Radical Scavenging and Anti-Isolated Human LDL Oxidation Activities of Pluchea indica (L.) Less. Tea Compared to Green Tea (Camellia sinensis).

Tea is one of the most popular beverages in the world. Camellia sinensis tea (CST) or green tea is widely regarded as a potent antioxidant. In Thailand, Pluchea indica (L.) Less. tea (PIT) has been commercially available as a health-promoting drink. This study focused on free radical scavenging activities of PIT, and its ability to protect isolated human low-density lipoproteins (LDL) from oxidation by chemical agents. A preliminary study to investigate the antioxidant nature of PIT was undertaken. These included common antioxidant assays involving 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), hypochlorous acid (HOCl), and its potential to scavenge peroxynitrite. In separated experiments, isolated human LDL was challenged with either 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), copper (Cu2+), or 3-Morpholinosydnonimine hydrochloride (SIN-1) to induce LDL oxidation. PIT exhibited antioxidant activity in all test systems and performed significantly better than CST in both DPPH (P < 0.05; IC50PIT = 245.85 ± 15.83 and CST = 315.41 ± 24.18 μg/ml) and peroxynitrite scavenging assays. PIT at 75 μg/ml almost fully prevented the peroxynitrite over a 5 h period. Moreover, it displayed similar properties to CST during the antioxidation of isolated human LDL using AAPH, Cu2+, SIN-1, and hypochlorous acid scavenging assays. However, it revealed a significantly lower ABTS scavenging activity than CST (P < 0.05; IC50PIT = 30.47 ± 2.20 and CST = 21.59 ± 0.67 μg/ml). The main constituents of the PIT were identified using LC-MS/MS. It contained 4-O-caffeoylquinic acid (4-CQ), 5-O-caffeoylquinic acid (5-CQ), 3,4-O-dicaffeoylquinic acid (3,4-CQ), 3,5-O-dicaffeoylquinic acid (3,5-CQ), and 4,5-O-dicaffeoylquinic acid (4,5-CQ). In conclusion, caffeoyl derivatives in PIT could play an important role in potent antioxidant properties. So, it may be further developed to be antioxidant beverages for preventing atherosclerosis and cardiovascular diseases associated with oxidative stress.

The assessment of electromagnetic fields-caused genetic diversity of Tea Camellia sinensis L. using ISSR and SCoT markers

The effects of electromagnetic fields on plants have been studied during the last decades. The electromagnetic fields as abiotic stress can induce genetic variation in living organisms including plants by different mechanisms. The aim of the present study is to assay the possible electromagnetic fields-caused genetic diversity of Tea Camellia sinensis L. which is known as favorite herbal beverages by many biochemical constituents. To do so, intersimple sequence repeats (ISSR) and start codon targeted (SCoT) loci were used as molecular markers. Different electromagnetic field treatments by 1, 2, 4 and 6 mT of intensity, for 30 and 60 min per day, for 7 continuous days were imposed, following by DNA extraction and PCR amplification. AMOVA, PCoA and STRUCTURE analysis based on the ISSR and SCoT markers showed that regardless of the EMFs intensity, 30 min of radiation causes more genetic variation than that of 60 min does until the intensity was increased up to 6 mT, due to direct and indirect mechanisms such as guanine modification, methylation of cytosines, Fenton reaction and direct DNA strands breaking. ISSR sequences, however, underwent more variation than SCoT.

Multifunctional Green Synthetic Hematite (α-Fe2O3) Nanoparticles Mediated by Camellia Sinensis (Indian Tea Plant) Extracts towards Antioxidant and Antiproliferative Activities against MCF-7 Cell Lines

Among nanoparticles used for medical applications, Camellia Sinensis Nanoparticles (CSNPs) are among the least investigated. This study was undertaken to develop CSNPs by green synthesis using Camellia sinensis tea (Theaceae) plant extract to produce the NPs. The Camellia sinensis, Indian tea plant used from ancient time to increase appetite. Other medicinal uses have also been employed for the synthesis of super paramagnetic α Fe2O3 nanoparticles (NPs). The plant extracts revealed the phenolic groups bifunctional nature and capping nature through the –OH bonding over the nanoparticles (NPs) surface. The prepared nanoparticles (NPs) shows α-Fe2O3 phase among iron oxides and spherical morphology with an average size around 5 nm. The magnetic measurements proved the superparamagnetic behaviour of NPs with non-saturating MS value of 8.5 emu/g at room temperature (300 K). The CSNPs were characterized by UV-V is spectroscopy and X-ray Diffractometry, and evaluated with Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR). The CSNPs were spherical (size 7-20 nm) and contained phenols and flavonoids acquired from the Camellia sinensis extract. CSNPs has good 1-Diphenyl-2-Picrylhydrazyl (DPPH), OH, and NO scavenging properties. MTT assay showed that CSNPs (IC50 = 0.006 μM) were more antiproliferative toward the human MCF-7 cells than the Camellia sinensis tea extract (IC50 = 0.894 μM), Gemcitabin (IC50 = 2.133 μM). The anticancer cell effects of CSNPs on MCF 7 are mediated through the induction of apoptosis and G2/M cell-cycle arrest.

Analytical strategy coupled to chemometrics to differentiate Camellia sinensis tea types based on phenolic composition, alkaloids, and amino acids.

Catechins, amino acids, and alkaloids are primary chemical components of tea and play a crucial role in determining tea quality. Their composition and content largely vary among different types of tea. In this study, a convenient chemical classification method was developed for six Camellia sinensis tea types (white, green, oolong, black, dark, and yellow) based on the quantification of their major components. Twenty-one free amino acids, 6 catechins, 2 alkaloids, and gallic acid in 24 teas were quantified using ultra-high-performance liquid chromatography (UHPLC). The total catechin contents in these tea samples ranged from 10.96 to 95.67 mg/g, while total free amino acid content ranged from 2.63 to 25.89 mg/g. Theanine (Thea) was the most abundant amino acid in all tea varieties. Catechin and amino acid levels in tea were markedly reduced upon fermentation of tea. Furthermore, high-temperature processing (roasting) during tea production induced degradation and epimerization of catechins, yielding epimerized catechins, simple catechins, and gallic acid. Principal component analysis revealed that major ester-catechins (EGCG and ECG), major amino acids (Thea), and major alkaloids (caffeine) are potential factors for distinguishing different types of tea. Linear discriminant analysis showed that 100% of teas were correctly classified in which (+)-catechin, ECG, EGC, gallic acid, GABA, cysteine, lysine, and threonine were the most discriminating compounds. This study shows that quantification of the major tea components combined with chemometric analysis, can serve as a simple, convenient, and reliable approach for classifying tea according to fermentation level. PRACTICAL APPLICATION: Different Camellia sinensis tea types can be produced worldwide but it is still challenging to know which chemical markers can be used to trace their production. in this paper we used a targeted methodology to classify six tea types (white, green, oolong, black, dark, and yellow) based on phenolic composition, alkaloids, and amino acids. The main chemical markers responsible for the discrimination were pinpointed with the use of chemometric tools.

Prospect of Application of Amendments in a Summer Rice Field: Upholding Soil Properties via Inorganic Blended Amendments

ABSTRACT The application of inorganic fertilizer in rice field is a foremost maneuver to acquire higher yield but effects properties of acidic sandy soil. An alternative strategy is to blend inorganic fertilizer with soil binding materials like magnesium sulfate (0.5 ton/ha), fresh neem (Azadirachata indica) (1 ton/ha) leaves, used tea (Camellia sinensis var assamica) (1 ton/ha) leaves, fresh karanj (Pongamia glabra) (1 ton/ha) leaves, and assess their prospects on soil properties and yield. The results of present investigation showed that the blended amendments exhibited improvement in iron, soil moisture, volumetric water content and bulk density. Inorganic fertilizer blended with karanj showed 4.12% increase in available phosphorus, 20.33% increase in available potassium, and 18.81% increase in zinc compared to inorganic fertilizer alone. Significant correlation between electrical potential and pH (−0.95), soil moisture and volumetric water content (0.93), soil temperature and copper (−0.90), bulk density and manganese (−0.88), and soil temperature and manganese (−0.88) were observed. Also 36.39% of variation in soil properties depended on electrical conductance, available phosphorus, iron, copper, manganese, soil temperature, and bulk density. However available phosphorus contributed 13.70% variation in yield.

Effects of Presoaking Treatments on Emergence of Tea (Camellia sinensis) Seeds

Effects of Presoaking Treatments on Emergence of Tea (Camellia sinensis) Seeds

Soil aggregation and aggregate-related exchangeable base cations under different aged tea (Camellia sinensis L.) plantations in the hilly regions of southern Guangxi, China

ABSTRACT As the secondary nutrients in soils, exchangeable base cations can form cationic bridges with clay particles and organic matters to promote the formation of soil aggregates, and to protect soil organic carbon (Corg) and nutrients from loss. To have a comprehensive understanding toward the dynamics of soil exchangeable base cations in the tea planting process, it requires to evaluate these cations’ distribution in soil aggregates at various sizes. In this study, soil samples (0–20 cm) from the tea plantations at various ages (8, 17, 25, and 43 yr.) in the hilly regions of southern Guangxi, China were divided into >2, 1–2, 0.25–1, and <0.25 mm aggregate fractions through a dry-sieving procedure. Then, the cation exchange capacity (CEC), total exchangeable bases (TEB), and different individual cations, such as exchangeable potassium (K+), sodium (Na+), calcium (Ca2+), and magnesium (Mg2+), in soil aggregates were detected. Our results suggested that the mean weight diameter (MWD), that is an indicator evaluating the stability of soil aggregates, in the 17-yr. tea plantations appeared to be the highest among all the tea plantations, indicating that more stable soil aggregates could be formed in the 17-yr. tea plantations in comparison with the rest plantations. During tea planting of 35 yr. (from 8 to 43 yr.), the increasing rate of soil CEC stock was 33.42 cmol m−2 yr.−1, while the loss rates of soil exchangeable K+, Na+, Ca2+, Mg2+, and TEB were 0.89, 0.52, 3.86, 1.23, and 6.51 cmol m−2 yr.−1, respectively. Interestingly, the loss rates of soil exchangeable base cations in the middle phase (from 17 to 25 yr.) were greater than those in other phases. Therefore, it is of critical importance to implement a sustainable management scheme to maintain soil aggregate stability and to minimize the loss of soil exchangeable base cations in the tea planting process, especially after 17 yr., in the hilly regions of southern Guangxi, China.

STIMULASI PERTUMBUHAN BIBIT TEH (Camellia sinensis) DENGAN PEMBERIAN URIN SAPI DAN PENGGUNAAN BAHAN STEK YANG BERBEDA

[STIMULATION EFFECTS OF BIOURINE CONCENTRATION AND THE STEM SEGMENT OF CUTTINGS ON GROWTH OF TEA (Camellia sinensis) SEEDLINGS]. Tea seedlings can be prepared by the stem segment cutting and provide treatment to stimulate the growth of cuttings. This study aims to determine the optimal tea seedlings growth from different material of stem segment and biourine concentration. The experiment was conducted in February to June 2020 at PT. Sarana Mandiri Mukti Kelurahan Tangsi Baru, Kabawetan District, Kepahiang Regency, Bengkulu Province. This research uses factorial completely randomized design (CRD) with two factors. The first factor, biourine concentration consisting of 5 levels including 0%, 25%, 50%, 75%, 100%. The second factor, the stem segment of cuttings consisting of 3, 4, and 5 segments. The treatment was repeated 3 times, with 5 plants sample in each treatment. There was totally 225 plants. The results showed that biourine concentration of 100% has significantly different from the variable roots number and root length. The stem segment of the tea cuttings has no significantly difference effect on all variables. The interaction between biourine concentration and stem segment of the tea cuttings has significantly different in variables of shoot length and seedlings fresh weight. The optimum growth of tea seedling was obtained in stem cutting of 3 segment and application of biourine concentration of 100%.

Bioleaching Solutions Used for the Nanoparticles Biosynthesis for Uranium and Arsenic Immobilization

Bioleaching solutions from uranium and arsenic solid waste served as a source of U(VI) and As (V) ions, while plant extract from green tea (Camellia sinensis) – as a reductor for nanoparticle synthesis. Uranium dioxide and As (III) nanoparticles were formed as a product of bioreduction on the hematite and schwertmannite surfaces respectively, which resulted in an increase in the negative zeta potential with an increase in pH. It suggests, that the electrical potential depends on the presence of nanoparticles. The SEM microphotographs revealed the UO2 nanoparticles on the hematite surface.

Predation Pressure in Tea (Camellia sinensis) Plantations in Southeastern China Measured by the Sentinel Prey Method.

Tea (Camellia sinensis) is an important food product with thousands of years of human use. Being a non-washable food, no pesticide residues are allowed, which increases the importance of natural means of plant protection. Predation, a component of natural pest control, is an important contributor to this, but its level and sustainability are not known in most of the areas of tea production. We quantified predation intensity using the artificial sentinel prey method in a tea-growing landscape containing remnants of the original forest vegetation in Fujian Province, China. The most common predators were chewing arthropods (49.8% of predation events) and birds (48.1%). Overall, predation rates at the edges of forest fragments (18.9% d-1) were lower than either in fragment interiors (25.4%d−1) or in the surrounding tea plantations (19.2–24.1%d-1). Arthropod predation was higher inside, and at the edge of, forest fragments than within plantations, and generally decreased with increasing distance from a fragment edge, indicating limited spillover of arthropod predators from the native habitat remnants to the cultivated matrix at the local scale. Bird predation, though, showed a different trend: it was lower on the inside of forest fragments than in the tea planation, and bird attack rates increased at increasing distances (up to 40 m) from the forest fragment edge. We also found a reciprocal relationship between attack rates by birds and arthropods, suggesting intra-guild predation. Measures protecting arthropod natural enemies could increase the combined pest suppression effect, contributing to pesticide-free tea production in China.

Modulation of Glucose Metabolism by Leaf Tea Constituents: A Systematic Review of Recent Clinical and Pre-clinical Findings.

Leaf teas are widely used as a purported treatment for dysregulated glucose homeostasis. The objective of this study was to systematically evaluate the clinical and cellular-metabolic evidence, published between January 2013 and May 2019, and indexed on PubMed, ScienceDirect, and Web of Science, supporting the use of leaf teas for this purpose. Fourteen randomized controlled trials (RCTs) (13 on Camellia sinensis teas) were included, with mixed results, and providing scant mechanistic information. In contrast, 74 animal and cell culture studies focusing on the pancreas, liver, muscle, and adipose tissue yielded mostly positive results and highlighted enhanced insulin signaling as a recurring target associated with the effects of teas on glucose metabolism. We conclude that more studies, including RCTs and pre-clinical studies examining teas from a wider variety of species beyond C. sinensis, are required to establish a stronger evidence base on the use of leaf teas to normalize glucose metabolism.

Introduction of two predators to control Dendrothrips minowai (Thysanoptera: Thripidae) in tea (Camellia sinensis) plantations in China

ABSTRACTDendrothrips minowai Priesner (Thysanoptera: Thripidae) is the main pest thrips in tea (Camellia sinensis) plantations in Guizhou Province, damaging tea shoots and leaves. In this study, we tested the control of the predatory mite Amblyseius cucumeris Oudermans and the ladybeetle Harmonia axyridis Pallas against D. minowai in the laboratory and tea plantations in Guizhou, China. The results showed that D. minowai suppression by A. cucumeris was 29.0% at the predator to prey ratio of 1:10 and 45.3% at 1:5, and by H. axyridis it was 45.0% at the 1:10 ratio and 67.2% at 1:5 in the laboratory. After the introduction of these two predators in tea plantations, during the period of highest density of D. minowai (between May and August), the release of A. cucumeris and H. axyridis reduced D. minowai by 26.3 and 56.7% in 2015 and by 40.3 and 54.5% in 2016, respectively. Densities of A. cucumeris and H. axyridis per 100 tea leaves ranged from 50.3 to 137.5 for the mite and from 18.0 to 65.0 for the ladybird beetle. Thus, A. cucumeris and H. axyridis can be used as effective predators in controlling D. minowai in Chinese tea plantations.

SEASONAL VARIATIONS IN ANTIOXIDANT CAPACITIES AND PHENOLIC CONTENTS OF TEA LEAF EXTRACTS

Objectives: The objective of the study was to estimate the seasonal variations in the antioxidant capacities, total polyphenol content (TPC), total flavonoid content (TFC), and tannin content (TC) of tea leaf extracts from two different plantation sites.&#x0D; Methods: Samples were collected from two tea gardens in Tuli and Ungma situated at N 26°39’19.3 E 094°39’22.7 and N 26°17’30.6 E 094°28’29.2, respectively, under the Mokokchung district of Nagaland, India. TPC, TFC, and TC from sample extracts were determined using Folin–Ciocalteu reagent, aluminum chloride colorimetric, and Folin–Ciocalteu assay. Apart from these, antioxidant capacities were analyzed using ferric reducing ability of plasma (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay.&#x0D; Results: The concentrations of total polyphenol, flavonoid, and tannin varied from 552.029±8.079 to 305.647±1.744 mg gallic acid equivalent/g, 238.770±0.508–148.457±1.653 mg catechin equivalent/g, and 26.453±0.485–20.173±0.173 mg tannic acid equivalent/g, respectively. FRAP and DPPH assay displayed value ranging from 2.564±0.023 to 1.074±0.023 mmol Fe(II) equivalent/g and 3.612±0.053–2.076±0.028 mmol Trolox equivalent/g. Significant seasonal variations in concentrations of these compounds were observed and a positive correlation between antioxidant capacities and phenolics of tea leaf extracts was established.&#x0D; Conclusion: Tea (Camellia sinensis (L.) O. Kuntze) has been regarded as a plant of immense medicinal and therapeutic value since time immemorial. The tea leaf extracts analyzed in this study gave high TPC, TFC, and TC, as well as high antioxidant activity in terms of DPPH and FRAP value. Studying such properties in tea leaves contributes more to our understandings of health benefit potentials in tea leaves and the quality of tea leaves on the basis of seasons and sites where they are planted.

Graft success, growth, and early flowering onset, as affected by grafting time in topworked tea plants

ABSTRACTTopworking method of grafting has immense practical applications in reducing the excessive time consumption in conventional breeding programs in tea (Camellia sinensis (L.) O. Kuntze). Hence, the present study was designed to know the influence of grafting time on growth and flowering in topworked scions. Scions from the clone TRF-4 were used for topworking on the field-grown plants of the clone UPASI-9. Topworking was performed during winter (January), pre-monsoon (May), and post-monsoon (September) seasons. The graft success rate was assessed 1 month after grafting. Twelve months after grafting, vigor of the topworked scions was evaluated by measuring length, number, and diameter of branches. Further assessments were carried out on the onset of flowering, length of the flowering period, and the number of flowers per scion during the flowering season. The results showed that the graft success rate, growth, and early onset of flowering were higher when topworking was done in May and September rather than in January. However, the flowering duration and number of flowers were significantly lower in the scions grafted in September. Therefore, topworking of suitable scions in May seems to be appropriate for attaining improved graft survival rate, early flowering onset, a higher number of flowers, and longer flowering duration in tea.

Scion-rootstock interactions in grafted tea at nursery and field levels

ABSTRACTGrafting of fresh tea (Camellia sinensis (L.) O. Kuntze) cuttings in the nursery offers the possibility to raise composite plants with superior traits. To increase the land productivity, grafted tea plants are greatly preferred in replanting programs in South India. Hence, the present study was conducted to widen the choice of compatible grafts and to know the consistency in nursery and field performances of grafted tea plants. The clone TRF-4 was used as scion for grafting on the rootstock clones UPASI-2, UPASI-6, UPASI-9, and UPASI-26. Nursery assessments showed that all grafted plants produced more vigorous composite plants than self-rooted TRF-4. However, field assessment indicated significant effects of stock-scion interaction on the performance of grafted plants. Significant increases in yield and canopy spread were noted when TRF-4 was grafted on UPASI-9, UPASI-2 and UPASI-26. However, composite plants of TRF-4 grafted on the rootstocks of UPASI-6 recorded lower field survival and yields compared to the self-rooted TRF-4. This result suggests that nursery performance is not a reliable index to judge the field performance of grafted plants.

Comparative histomorphological assessment of Vitamin E and Green tea (Camellia sinensis) extract-mediated amelioration of Lead-induced hepatopathy in experimental Wistar rats

Objective: Hepatotoxins such as lead has potency of distorting hepatic histomorphology. Conversely, antitoxins exhibit anti-hepatotoxic effects by ameliorating their damaging effects. This study was carried out to comparatively assess ameliorative effects of Vitamin E and green tea extract on hepatic histomorphology of rat model of lead-induced hepatopathy. Methods: Forty two (n=42) animals were equally grouped into six: Group A received 5 ml/kg distilled water; Group B received 2 mg/ml lead acetate; Groups C and D received 2 mg/ml lead acetate + 100 mg/kg and 200 mg/kg Vitamin E respectively; Groups E and F received 2 mg/ml lead acetate + 5 mg/kg and 10 mg/kg green tea extract respectively. All treatment was via oral route and lasted for 35 days wherein animal body weight was regularly recorded. Serum ALT and AST levels were determined; hepatic tissues were harvested, weighed and processed for histomorphological study. Data were analyzed using IBM-SPSS (version 20) and compared using t-test and analysis of variance. Results: There was significant (p < 0.05) body and organ weight decrease in Group B relative to Group A while Groups C-F only showed marginal reduction. Also, the serum ALT and AST levels were significantly (p < 0.05) increased in Group B while Groups C-F animals only showed marginal increase. The hepatic histomorphological features Groups C-F showed mild variations from normal histomorphology of Group A but intense variations were observed in Group B. Conclusion: Vitamin E and green tea extract comparatively exhibited histomorphological reparation against damaging effects of lead on hepatic histomorphology. The most potent dosages for their hepato-reparative activity were 200 mg/kg and 10 mg/kg respectively.

Evaluación de la capacidad antimutagénica de extractos etanólicos de Té verde (Camellia sinensis)

En la actualidad, las plantas medicinales contienen principios activos, que si bien son los responsables de las propiedades terapéuticas que se les atribuyen, también lo son de las intoxicaciones y reacciones adversas que pueden aparecer si se emplean en dosis inadecuadas o por períodos prolongados. Por lo tanto, el objetivo del presente trabajo es evaluar la posible capacidad antimutágena del extracto etanólico de té verde (Camellia sinensis) sustentado en su alto contenido antioxidante reportado. Para ello, se determinó la actividad antimutagénica mediante la prueba de mutación y recombinación somática (SMART), con un tratamiento previo de extractos etanólicos de té verde y tratamiento subsecuente con un mutágeno de referencia (O-Toluidina), evaluando la inhibición de manchas o clones celulares en ojo de Drosophila melanogaster. En cuanto a los resultados, se observó que el extracto etanólico administrado previamente al mutágeno de referencia, inhibió de manera significativa el daño genotóxico, siendo más efectivo para manchas pequeñas que grandes. En general, los resultados sugirieron que los extractos de té verde evaluados, tienen propiedades biológicas con un potencial benéfico para la salud humana y que se pueden utilizar en tratamientos preventivos contra el cáncer o exposición relacionadas con mutágenos de manera laboral o naturales.

Kombucha Tea Production Using Different Tea Raw Materials

Tea (Camellia sinensis) is an agricultural product that is widely issued by people around the world. Some types of tea in the world are green tea, black tea, oolong tea and white tea. Kombucha tea is brewed water for tea and sugar then given bacteria and fungi and fermented for 7-10 days. The purpose of this study was to determine the quality of kombucha of several different types and determine the organoleptic value of fermented kombucha tea using different types. This research was conducted in April-July 2019 in the Microbiology Laboratory of the Department of Biology FMIPA Padang State University. This research is an experimental study with 5 treatments and 1 repetition (K: kombucha starter, P1: kombucha starter + green tea, P2: kombucha starter + black tea, P3: kombucha starter + oolong tea, P4: kombucha starter + white tea. Parameters Calculated are the number of microbes, nata thickness, pH of kombucha tea, total acetic acid, and organoleptic tests of kombucha tea.The results of this study indicate differences in the types of tea used in kombucha fermentation, the quality of kombucha tea produced, kombucha tea using black tea. The organoleptic test requested by the panelists was kombucha tea from white tea.