California Research Articles

Impact of COVID-19 on total hip arthroplasty: results from California state inpatient database

Background: COVID-19 pandemic has significantly affected orthopedic surgery, resulting in postponements and cancellations. The aim of this study is to determine the possible effects of COVID-19 on the number of total hip arthroplasty (THA) hospitalizations and hospital outcomes using a large database. Methods: We conducted a retrospective analysis of data gathered and stored in the California State Inpatient Database (SID) during 2019 and 2020. All THA hospitalizations ≥18 yr that occurred in 2019 and 2020 and COVID-19 hospitalizations that occurred in 2020 were used for the analysis. The primary outcomes of the study were trends in THA hospitalizations between 2019 and 2020. Secondary outcomes were mortality, surgical, medical, and other complications, and prolonged length of stay. Results: A total of 36,760 and 20,243 THA hospitalizations occurred during 2019 and 2020, respectively. Trends in THA hospitalizations during 2019 and 2020 showed that the rates of these hospitalizations were substantially lower throughout 2020. Logistic regression analysis showed that the odds of mortality (odds ratio [OR], 2.77; 95% CI, 1.38-5.53), surgical complications (OR,1.61; 95% CI, 1.36-1.89), medical complications (OR,1.27; 95% CI, 1.20-1.33), other complications (OR,1.42; 95% CI, 1.19-1.69), and prolonged length of stay (OR,1.10; 95% CI, 1.05-1.16) were significantly higher during 2020. Conclusions: Our findings show that during COVID-19 pandemic THA hospitalizations were prioritized based on case severity. By the end of 2020 THA hospitalizations did not steeply decrease in numbers compared to the advent of the pandemic, indicating that orthopedic surgeons and other healthcare professionals functioned optimally even during the COVID-19 pandemic.

Integrating social media advertising into Giving Day fundraising

This paper explores the implementation and outcomes of a Giving Day paid social media advertising campaign conducted by California State University San Marcos (CSUSM). The objective of the campaign was to increase website traffic, donors and dollars raised. The paper highlights the importance of buy-in from leadership and the involvement of a dedicated team working within a set budget. The team utilised social media platforms, including Meta (Facebook and Instagram) and LinkedIn, for targeted advertising. A three-phase approach was adopted, focusing on raising awareness, driving traffic and converting audiences into donors. The team employed tracking methods such as Urchin Traffic Modules (UTMs) and custom links to measure the impact of the campaign. The results showed significant increases in website traffic, donors, first-time donors and dollars raised. Despite challenges related to tracking limitations and the need for extended ad duration, the team devised solutions, including implementing alternative tracking methods and extending the campaign duration. The paper also offers practical takeaways for creating paid social media advertising campaigns, emphasising the importance of research, planning, content creation, goal setting, target audience determination, tracking setup, campaign launch, monitoring and result analysis. Overall, the paper provides insights into the effectiveness of paid social media advertising in the context of a Giving Day campaign and offers strategies for optimising future campaigns.

Court rules against former director

Case name: Martin v. Board of Trustees of the California State University, No. B303509 (Cal. Ct. App. 11/14/23).

Prioritizing resource allocation to reduce adverse effects of pesticide risk for endangered species

The use of pesticides promotes food security because of the multiple benefits it brings to agriculture, such as reduction in crop losses. However, the use of pesticides can be potentially harmful to non-target species. In the U.S., the Environmental Protection Agency regulates the use of pesticides to manage the risks associated with these agents and to protect species under the Endangered Species Act. As part of these regulations, pesticides must be registered and then reviewed every 15 years to ensure the use conditions are updated with the best available data. The registration and review process can invoke corrective measures to ensure protection of endangered species. However, the registration review process is highly resource and time consuming. There is currently a backlog of unreviewed pesticides, leaving a large quantity of pesticides without updated use conditions to protect species. Identifying ways to streamline this process is urgently needed. We develop a sequencing approach to address the risk assessment bottleneck in the pesticide registration and review process and identify species that would benefit most from detailed assessments. We then demonstrate the magnitude of potential efficiencies using this sequencing process for 61 terrestrial listed species in the state of California. Our results show a consistent ranking of listed species according to their relative benefits from assessment, with 90 % of the species being robustly classified across scenarios in the sensitivity analysis. We found that prioritizing the assessment of a small group of species could potentially result in high conservation benefits, and identify species in need of more detailed data for a robust sequencing. We examine how a sequencing approach can guide decisions about what species might benefit most from different levels of assessment. Our results demonstrate the conservation benefits of employing a sequencing approach to prioritize the allocation of limited resources for endangered species.

Disparities in pre-health advising across California's public universities.

The Supreme Court's decision in Students for Fair Admissions, Inc., v. Harvard College is likely to result in the matriculation of fewer students from historically excluded racial/ethnic groups at more selective colleges and universities and matriculation of more students at less selective colleges and universities. Because of this, it is important to understand how resources for pre-health advising, a modifiable factor that can help increase the diversity of the health workforce, vary across institutions with differing levels of selectivity. Colleges are known to vary in resources, structure, and investment in pre-health advising but data are lacking and there is no estimate of any pre-health advising resource gap. To quantify availability of advising resources and identify perceived challenges in pre-health advising in California's highly diverse public and select private undergraduate institutions. Structured 60-minute Zoom interviews from June 2022 -October 2022 at 18/23 CSU (California State Universities), 9/9 University of California (UC) institutions and 6 select private institutions with varying levels of selectivity. Two investigators independently analyzed interviews using a Grounded Theory Approach. The full study team reviewed transcripts and themes. Pre-health advisor capacity varied greatly across the three types of institutions. CSU: mean = 1 FTE advisor: 24,620 graduates (range: 1: 1,059-1: 150,520); UC mean = 1 FTE advisor: 4,526 graduates (range: 1: 1,912-1: 10,920); private institutions mean = 1 FTE advisor:1,794 graduates (range: 1: 722-1: 5,300). Participants reported common challenges: advising capacity, lack of advisor training, advisor turnover, and student difficulties in accessing clinical opportunities and required coursework. CSU and UC participants noted that these had greatest impact for first generation and racially/ethnically underrepresented students for whom lack of informal professional networks, lack of other mentors, and financial responsibilities complicate college navigation and professional school application. Students at CSU campuses had 5 times less access to pre-health advising per graduate than UC students, and 13 times less than students at private institutions. Much greater investment is needed in California's public institutions, particularly CSUs, to increase equity in access to advising for pre-health professional students. Research should examine pre-health advising resource capacity in other states, especially those that are now facing race-neutral admissions policies at undergraduate institutions and health professions schools.

Latinx Immigrant Health: Does Immigrants’ Perceptions of Integration Protect their Health?

Restrictive immigration policies and enforcement practices are associated with poor health and mental health outcomes among Latinx immigrants. Over the last two decades, the state of California has enacted over 20 pro-integration immigration policies to protect and enhance the quality of life for immigrants. Accounting for the nested structure of immigration policy, this study examined the relationship between immigrants’ perceptions of integration and health. Participants originated primarily from Mexico ( N = 137). Hierarchical regression analysis was used to evaluate the contribution of variables in sequential blocks. While controlling for demographics, and immigration characteristics, we assessed the relationship between California immigration policy knowledge, perceived immigrant integration, threat of deportation, and two indicators of health—psychological distress and self-rated health. Perceived immigrant integration was associated with lower levels of psychological distress and better self-rated health. The threat of deportation was associated with a decline in self-rated health and higher levels of psychological distress. Policy advocacy and access to health care are needed to reduce the harmful effects of enforcement practices.

ECR Spotlight – Serena Farrell

ECR Spotlight is a series of interviews with early-career authors from a selection of papers published in Journal of Experimental Biology and aims to promote not only the diversity of early-career researchers (ECRs) working in experimental biology but also the huge variety of animals and physiological systems that are essential for the ‘comparative’ approach. Serena Farrell is an author on ‘ Voltage-gated ion channels are expressed in the Malpighian tubules and anal papillae of yellow fever mosquito Aedes aegypti and may regulate ion transport during salt and water imbalance’, published in JEB. Serena conducted the research described in this article while a undergraduate student in Dennis Kolosov's lab at California State University San Marcos, USA. She is now a PhD student in the lab of Spencer Behmer at Texas A&M University, USA, investigating the molecular physiology of insects and how they interact with their environment.

Gender Differences in Hospital Outcomes among COVID-19 Hospitalizations.

Many epidemiological studies have shown that coronavirus disease 2019 (COVID-19) disproportionately affects males, compared with females, although other studies show that there were no such differences. The aim of the present study was to assess differences in the prevalence of hospitalizations and in-hospital outcomes between the sexes, using a larger administrative database. We used the 2020 California State Inpatient Database for this retrospective analysis. International Classification of Diseases, Tenth Revision, Clinical Modification diagnosis code U07.1 was used to identify COVID-19 hospitalizations. These hospitalizations were subsequently stratified by male and female sex. Diagnosis and procedures were identified using the International Classification of Diseases, Tenth Revision, Clinical Modification codes. The primary outcome of the study was hospitalization rate, and secondary outcomes were in-hospital mortality, prolonged length of stay, vasopressor use, mechanical ventilation, and intensive care unit (ICU) admission. There were 95,180 COVID-19 hospitalizations among patients 18 years and older, 52,465 (55.1%) of which were among men and 42,715 (44.9%) were among women. In-hospital mortality (12.4% vs 10.1%), prolonged length of hospital stays (30.6% vs 25.8%), vasopressor use (2.6% vs 1.6%), mechanical ventilation (11.8% vs 8.0%), and ICU admission rates (11.4% versus 7.8%) were significantly higher among male compared with female hospitalizations. Conditional logistic regression analysis showed that the odds of mortality (odds ratio [OR] 1.38, 95% confidence interval [CI] 1.38-1.44), hospital lengths of stay (OR 1.35, 95% CI 1.31-1.39), vasopressor use (OR 1.59, 95% CI 1.51-1.66), mechanical ventilation (OR 1.62, 95% CI 1.47-1.78), and ICU admission rates (OR 1.58, 95% CI 1.51-1.66) were significantly higher among male hospitalizations. Our findings show that male sex is an independent and strong risk factor associated with COVID-19 severity.

An Arduous Journey of Early Chinese Americans

After the state of California discovered gold in 1848, a gold rush immediately rolled out and spread across North America. But most laborers from the east coast did not want to go to California and only the white Irish workers were drawn to the West because they were smeared as “dirty whites” in the east coast. To attract more laborers to toil in the gold mines, employers began seeking immigrants from China. Unlike white workers, Chinese laborers did not want to stay in the US for very long time and all they wanted was making enough money to buy homes and marry a woman in China. They were willing to work longer time for less, which draw ire from their fellow white workers. Because white workers had voting rights, congressmen in California sided with white workers against Chinese laborers by passing a number of discriminative laws that culminated in the 1882 Chinese Exclusion Act. The law virtually banned all Chinese laborers to enter the US until 1943 when China became a wartime ally of the US. Some Chinese including Wong Kim Ark successfully challenged the law by capitalizing on the US legal system. The long and difficult journey of Chinese immigrants finally ran its course in the 1960s thanks to the Civil Right Movement.

School Websites: Local Contexts of Physical Activity and Physical Education Information

As the digital age continues to evolve, websites provide a potential way for physical education (PE) programs and schools to offer information on how they handle the topic of physical activity (PA). Charter schooling and charter schools have become substantially prevalent over the last 30 years, and the trend does not appear to be slowing. A variety of studies to gather information about PE and PA have already been conducted (Hill et al., 2010; Kahan & McKenzie, 2020; Kahan & McKenzie, 2021; Kahan et al., 2019). Specifically, Kahan and McKenzie have performed a variety of website analyses in the state of California, content-analyzing the sites to surveil PA and PE information on school websites. Further, they have looked at school factors such as local demographics, school size, religious affiliation, and public/private/charter affiliation. By following an intended partial-replication protocol, we looked to surveil Arizona-based charter schools to study the local context of another charter “hotspot.” We collected data through data collection protocols communicated by Dr. Kahan (circa June 2019). By conducting a quantitative descriptive study, we looked at characteristics and ran binary regression models to look for inferentially underlying trends. Descriptively, most findings mirrored those of previous research, with marginal presence of any information specific to PE and PA. We found significant predictors for website information for the categories of sports, PE frequency, presence of a PE teacher, school levels, and recess. We found similarities and differences between school types, but these targeted certain factors that may be expanded on or used to guide future website analyses both in Arizona and nationally. This is significant because as the prevalence of charter schools continues to grow, it is important to keep students sufficiently physically active and physically educated throughout a school day.

Professional Development to Improve Responsible Beverage Service Training: Formative Research Results and Protocol for a Randomized Controlled Trial.

Improved interventions are needed to reduce the rate of driving while intoxicated. Responsible beverage service (RBS) training has reduced service to intoxicated patrons in licensed premises in several studies. Its efficacy might be improved by increasing the proper application and continued use of RBS with a professional development program in the 3 to 5 years between the required RBS retraining. This study aims to develop and evaluate a professional development component for an RBS training that aims to improve the effectiveness of the web-based training alone. In a 2-phase project, we are creating a professional development component for alcohol servers after completing an RBS training. The first phase involved formative research on the feasibility, acceptability, and potential effectiveness of components. Semistructured interviews with owners and managers of licensed establishments and focus groups and a survey with alcohol servers in New Mexico and Washington State examined support for RBS and the need for ongoing professional development to support RBS. A prototype of a professional development component, WayToServe Plus, was produced for delivery in social media posts on advanced RBS skills, support from experienced servers, professionalism, and basic management training. The prototype was evaluated in a usability survey and a field pilot study with alcohol servers in California, New Mexico, and Washington State. The second phase of the project will include full production of the professional development component. It will be delivered in Facebook private groups over 12 months and evaluated with a sample of licensed premises (ie, bars and restaurants) in California, New Mexico, and Washington State (n=180) in a 2-group randomized field trial (WayToServe training only vs WayToServe training and WayToServe Plus). Licensed establishments will be assessed for refusal of sales to apparently intoxicated pseudopatrons at baseline and 12 months after the intervention commences. Although owners and managers (n=10) and alcohol servers (n=43) were favorable toward RBS, they endorsed the need for ongoing support for RBS for servers and identified topics of interest. A prototype with 50 posts was successfully created. Servers felt that it was highly usable and appropriate for themselves and the premises in the usability survey (n=20) and field pilot test (n=110), with 85% (17/20) and 78% (46/59), respectively, saying they would use it. Servers receiving the professional development component had higher self-efficacy (d=0.30) and response efficacy (d=0.38) for RBS compared with untreated controls. Owners, managers, and servers believed that an ongoing professional development component on RBS would benefit servers and licensed premises. Servers were interested in using such a program, a large majority engaged with the prototype, and servers receiving it improved on theoretic mediators of RBS. Thus, the professional development component may improve RBS training. ClinicalTrials.gov NCT05779774; http://tinyurl.com/4mw6d2vk. DERR1-10.2196/49680.

Comprehensive Sexual Health Educational Learning Modules for College Students.

Sexual health education in the United States continues to be a controversial topic of discussion with no federal regulations. Due to this, the sex education students receive before college varies greatly. Our team discovered that no mandatory, comprehensive sexual health education is required for students once they enter the California State University system. At Cal Poly specifically, it was found that students were engaging in risky sexual behaviors. For example, the 2021 American College Health Association-National College Health Assessment III Fall 2021 Data Report revealed that less than half (42.1%) of students reported regularly using condoms during vaginal sex, and a mere 5.1% of students reported barrier method use during oral sex. To fill in the noticed gaps in our students' sexual health education, our team developed a set of 10 comprehensive sexual health educational learning modules. The modules cover topics ranging from condom use and STIs to pleasure, porn literacy, and having difficult conversations with a partner. This anonymous online resource provides students with easy-to-read written material and engaging graphics. Future research will focus on user engagement and the effect of the resource on sexual health best practices across our campus.

The gender stereotyping of entrepreneurship

The gender stereotyping of entrepreneurship Professors Jennings and Tonoyan distill prior research and chart avenues for future research. In two prior Open Access Government articles, Dr Jennifer Jennings from the University of Alberta and Dr Vartuhi Tonoyan from California State University, Fresno, shared findings from their research (with collaborator Dr Robert Strohmeyer from the University of Mannheim) on how sex-based labour market segregation affects women’s perceptions of entrepreneurship and innovativeness as entrepreneurs. Here, they summarise key takeaways from their review of the academic literature on the gender stereotyping of entrepreneurship.

Evaluating the performance of WRF in simulating winds and surface meteorology during a Southern California wildfire event

The intensity and frequency of wildfires in California (CA) have increased in recent years, causing significant damage to human health and property. In October 2007, a number of small fire events, collectively referred to as the Witch Creek Fire or Witch Fire started in Southern CA and intensified under strong Santa Ana winds. As a test of current mesoscale modeling capabilities, we use the Weather Research and Forecasting (WRF) model to simulate the 2007 wildfire event in terms of meteorological conditions. The main objectives of the present study are to investigate the impact of horizontal grid resolution and planetary boundary layer (PBL) scheme on the model simulation of meteorological conditions associated with a Mega fire. We evaluate the predictive capability of the WRF model to simulate key meteorological and fire-weather forecast parameters such as wind, moisture, and temperature. Results of this study suggest that more accurate predictions of temperature and wind speed relevant for better prediction of wildfire spread can be achieved by downscaling regional numerical weather prediction products to 1 km resolution. Furthermore, accurate prediction of near-surface conditions depends on the choice of the planetary boundary layer parameterization. The MYNN parameterization yields more accurate prediction as compared to the YSU parameterization. WRF simulations at 1 km resolution result in better predictions of temperature and wind speed than relative humidity during the 2007 Witch Fire. In summary, the MYNN PBL parameterization scheme with finer grid resolution simulations improves the prediction of near-surface meteorological conditions during a wildfire event.

Long‐term compost use and high frequency low concentration fertigation reduce N2O emissions from a California almond orchard

AbstractUsing compost as an agricultural amendment offers a means to reduce organic waste, as mandated in the US state of California (SB 1383). Compost can improve soil physical characteristics and soil carbon content by increasing soil organic matter. We examined the effect of compost application (7‐year duration) on soil nitrous oxide (N2O) emissions, inorganic nitrogen (N) pools, soil temperature and water content, bulk density, and total carbon (C) and N content. The orchard was composed of Nonpareil cultivars interplanted with Aldrich and Carmel cultivars, all grafted on Nemaguard peach rootstock [Prunus persica (L.) Bratsch]. Its soil was Oakdale sandy loam (coarse‐loamy, mixed, active, thermic Mollic Haploxeralf). Delivering nutrients through the irrigation system, the orchard was fertigated 14 times with urea ammonium nitrate or calcium ammonium nitrate, using high frequency and low concentration (HFLC) fertigation applications (total 195 kg N ha−1). Soil without compost (No Compost) tended to have higher N2O fluxes (up to 2.75‐fold) than soil with compost (Compost). Cumulative measured N2O emissions (i.e., sum of nine sampling events) from Compost were half that from No Compost. Soil pH, electrical conductivity, total C, and N tended to be greater in Compost, and bulk density tended to be lower in Compost than No Compost. No relationships between N2O emissions and soil temperature, volumetric water content, water‐filled pore space, and inorganic N pools occurred in either treatment. Our findings indicate that long‐term applications of compost in perennial crops, in combination with an HFLC nutrient management program, could reduce losses of N as N2O to the atmosphere.

Sales to Apparently Intoxicated Customers in Three States With Different Histories of Responsible Beverage Service Training.

Most states prohibit sales of alcohol to customers who are apparently intoxicated, and many require training in responsible beverage service (RBS), with the aim of reducing driving while intoxicated (DWI) and other harms. Sales to apparently intoxicated patrons were assessed in onsite alcohol sales establishments and compared across three states. A sample of 180 licensed onsite alcohol establishments was selected in California (n = 60), New Mexico (n = 60), and Washington State (n = 60). States had different RBS training histories, content, and procedures. Research confederates, trained to feign cues of intoxication, visited each establishment twice. The pseudo-intoxicated patron (PP) ordered an alcoholic beverage while displaying intoxication cues. Sale of alcohol was the primary outcome. At 179 establishments assessed, PPs were served alcohol during 56.5% of 356 visits (35.6% of establishments served and 22.6% did not serve at both visits). Alcohol sales were less frequent in New Mexico (47.9% of visits; odds ratio [OR] = 0.374, p = .008) and Washington State (49.6%; OR = 0.387, p = .012) than in California (72.0%). Servers less consistently refused service at both visits (6.8%) in California than New Mexico (33.9%) or Washington (27.1%), χ2(4, n = 177) = 16.72, p = .002. Alcohol sales were higher when intoxication cues were less obvious (p < .001). Overservice of alcohol to apparently intoxicated customers was frequent and likely elevated risk of DWI and other harms. The lower sales in New Mexico and Washington than California may show that a policy approach prohibiting sales to intoxicated customers combined with well-established RBS training can reduce overservice. Further efforts are needed to reduce overservice.

COVID-19 vaccination uptake and receptivity among veterans enrolled in homelessness-tailored primary health care clinics: provider trust vs. misinformation

BackgroundCompared to the general population, individuals experiencing homelessness are at greater risk of excess morbidity and mortality from COVID-19 but have been vaccinated at lower rates. The U.S. Department of Veterans Affairs (VA)’s Homeless Patient Aligned Care Team (HPACT) program integrates health care and social services for Veterans experiencing homelessness to improve access to and utilization of care.MethodsThis study explores the vaccination uptake behavior and attitudes through a qualitative comparative case study of two HPACT clinics, one in California (CA) and one in North Dakota (ND). Semi-structured telephone interviews were conducted with Veterans enrolled in the two VA HPACT clinics from August to December 2021 with 20 Veterans (10 at each clinic).ResultsFour themes emerged from the interviews: (1) Vaccination uptake and timing— While half of the Veterans interviewed were vaccinated, ND Veterans were more likely to be vaccinated and got vaccinated earlier than CA Veterans; (2) Housing— Unsheltered or precariously housed Veterans were less likely to be vaccinated; (3) Health Care— Veterans reporting positive experiences with VA health care and those who trusted health providers were more likely to vaccinate than those with negative or nuanced satisfaction with health care; (4) Refusers’ Conspiracy Theories and Objectivity Claims— Veterans refusing the vaccine frequently mentioned belief in conspiracy theories while simultaneously asserting their search for objective information from unbiased sources.ConclusionsThese findings amplify the importance of improving access to population-tailored care for individuals experiencing homelessness by reducing patient loads, expanding housing program enrollment, and increasing the provider workforce to ensure personalized care. Health care providers, and housing providers, social workers, and peers, who offer information without discrediting or criticizing Veterans’ beliefs, are also key to effectively delivering vaccine messaging to this population.

Innovative Pedagogy Through Big Data and ArcGIS: Perspectives on the Collaboration Among First-Generation Students, Nonprofits, and Open Data in Los Angeles

This article focuses on the innovative teaching practices using big data that arose from a 4-year collaboration between faculty, first-generation students at California State University, Los Angeles (CSULA), and local communities. The aim of the National Science Foundation–funded Big Data Project at CSULA was to engage students and faculty in using big data to tackle social issues such as racial violence, poverty, environmental inequalities, urban ecology, and structural barriers in Los Angeles, while preserving the cultural richness of the communities in East Los Angeles and surrounding areas. Once a year, faculty members applied to the program to incorporate data projects into their courses, received training, adapted their curricula, and worked with nonprofits. Our experience showed that the students who participated in this semester-long, hands-on learning experience exceeded the learning objectives of the program and specific courses. They were key contributors in generating new insights using big data, fostering meaningful dialogue, and creating research and scholarship opportunities that positively impacted their communities.

First report of Globisporangium uncinulatum (syn. Pythium uncinulatum) causing Pythium wilt of lettuce in Arizona.

In 2021 and 2022, wilt symptoms were observed in lettuce (Lactuca sativa L.) fields in Yuma County, Arizona (AZ). Incidence was < 1% at all locations. Symptoms included stunting, yellowing and wilting of outer leaves. As disease progressed, outer leaves wilted and turned necrotic. In advanced stages, tap roots turned brown-gray, with few feeder roots. The crown remained intact until the plant collapsed. Symptomatic romaine and iceberg plants were collected from two sites. Necrotic roots were washed and then plated on amended corn meal agar (PARP) (Kannwischer et al. 1978). After 2-3 days, slow growing, coenocytic, submerged mycelia grew from these pieces. In culture, profuse oogonia formed with diameters of 30-39 (avg. = 33.7) µm and spiny protuberances (5-8 [avg. = 6.4] µm long) with thickened bases. Oospores were spherical and aplerotic, with diameters of 25-32 (avg. = 27.8) µm. Lettuce with identical symptoms from the Salinas Valley, California (CA) were also tested and similar isolates were recovered. Pathogenicity was tested using six AZ and one CA isolates. Inoculum was grown on barley seeds moistened with sterile distilled water, autoclaved three times (at 24 h intervals), then inoculated with colonized agar plugs and incubated at 20°C. Inoculum was used after two weeks. For each isolate, 12 3-week-old iceberg (cv. Speedway) and romaine (cv. Del Sol) plants were inoculated by placing 3-4 colonized barley seed next to the roots of the potted plants. Plants were maintained in a greenhouse at 24-26°C (daytime high) with ambient light. After eight days, all inoculated plants exhibited chlorotic lower leaves that later wilted. Both feeder roots and taproots showed brown-gray discoloration and were necrotic. Microscopy showed the presence of spiny oogonia in inoculated roots. Symptoms caused by the AZ and CA isolates were indistinguishable from each other. Isolations from necrotic tissue resulted in colonies morphologically identical to the original isolates. Twelve control plants inoculated with uncolonized barley seed developed no symptoms. DNA was extracted from all seven AZ and CA isolates pre-inoculation, and AZ isolate 2 recovered from both lettuce types post-inoculation for molecular characterization. The internal transcribed spacer (ITS) and cytochrome C oxidase subunit 2 (COX II) were amplified for the above isolates using primer sets ITS1/ITS4 (White et al. 1990) and FM66/FM58 (Villa et al. 2006), then sequenced. ITS of pre- and post-inoculated isolates for AZ (OQ054806 and OQ054807) and CA (OQ564388) matched 1078/1078 bases of Globisporangium uncinulatum (syn. Pythium uncinulatum; AY598712.2) with 99.8% identity. There were two SNPs in COX II for AZ isolate 1 (OR069239); all other isolates pre- and post- inoculation for AZ (OR069240 and OR069242) and CA (OR069241) uniformly matched 533/535 bases of G. uncinulatum (KJ595385.1) with 99.4% identity. Based on these molecular and morphological data, the isolates were identified as G. uncinulatum (Blok and Van Der Plaats-Niterink 1978; Van Der Plaats-Niterink 1981). To our knowledge, this is the first report of G. uncinulatum on lettuce in AZ. Designated as Pythium wilt, this disease is reported on lettuce in The Netherlands (Blok and Van Der Plaats-Niterink 1978), Japan (Matsuura, et al. 2010), and CA (Davis, et al. 1995). Arizona is an important lettuce growing region; if this disease becomes more prevalent, lettuce production in this region could be negatively impacted.

Migração e mortalidade por câncer entre os migrantes colombianos nos EUA: um estudo com dados de declaração de óbito

Abstract We aim to compare cancer mortality rates of USA Colombian migrants (USA Colombians) to Colombians in their country of origin (CO Colombians). Using Colombian national mortality data and data on cancer deaths among Colombians residing in the states of California, Florida, and New York (USA Colombians) for the period 2008-2012, we estimated sex-specific and age-standardized mortality rates (ASMR), expressed per 100,000 persons. For comparisons between the two populations before and after adjustment for educational level, negative binomial regression models were used to compute Mortality Rate Ratios (MRR). CO Colombians had higher cancer mortality rates compared with USA Colombians (male MRR 1.4 [95%CI: 1.2-1.5], female MRR 1.5 [95%CI: 1.3-1.7]). These differences persisted for most cancers even after adjustment for education. CO Colombians had significantly higher mortality from gastric (MRR 2.6 in males and 2.8 in females) and cervical cancer (MRR 5.0) compared with US Colombians. Educational inequalities in cancer mortality were more pronounced among CO Colombians than among USA Colombians. Lower cancer mortality observed among USA Colombians cannot be attributed to differences in education, an indicator of socio-economic status. Rather, it is likely due to better access to preventive and curative healthcare in the USA.