Calcium Deficiency Research Articles

Health risks from desalinated seawater used for human consumption: The case of Honaine Plant (Northwest Algeria)

Background: Honaine desalination plant produces 200,000 m3/day of drinking water, and applies a reverse osmosis process with sand/anthracite filters for pre-treatment. Objective: This study aim to explore the quality of treated water (TW) and to address any potential health risks. Methods: Samples of TW were collected daily, three times during the same period, for one month to investigate the physico-chemical characteristics of desalination. They were analyzed for calcium, magnesium, pH, alkalinity, TDS, free-chlorine, hardness, nitrite and sulfate. Results: After comparing the standards set by WHO and Algeria, the results showed that the TW of Honaine plant had low-mineral content, with a noticeable deficiency of calcium (21.22 ± 0.6 mg L-1) magnesium (1.74 ± 0.26 mg L-1), and sulfate (0.02 ± 0.01 mg L-1). The levels of chlorine (0.53 ± 0.08 mg L-1) and nitrites (0.003 ± 0.00 mgL-1) were lower than what the guidelines prescribe. The alkalinity and hardness of the water categorized it as being moderately hard. As for the taste, the TDS value (289.24 ± 26.86 mgL-1) suggests that the TW is excellent but the presence of chlorine negatively influences its palatability. The values of alkalinity value (63.73 ± 1.05 mgL-1) and pH (8.42 ± 0.021) of TW remain in line with recommendations. The hardness value (60.32 ± 0.73 mgL-1) shows that TW is moderately hard and softer than standards. Conclusion: Our findings showed that desalinated TW reflected significant low-mineral content of many healthy elements.

Exploring the Role of Calcium in the Physiology of Tulipa: A Comparative Study across Different Cultivars

Cut tulip flower production, typically cultivated using hydroponic systems, often faces the challenge of stem toppling due to calcium (Ca) deficiency in the nutrient solution. Growers generally grow various tulip cultivars to meet market demands. In hydroponic production, plants require precise nutrient concentrations to promote growth and development, as the food reserves in the mother bulb are insufficient and vary depending on the plant cultivar. This study aimed to assess the impact of different tulip cultivars and Ca deficiency on tulip growth, flowering, and physiological changes. Two cultivars of tulip, namely, ‘Strong Gold’ and ‘Orange Juice’, were subjected to three distinct nutrient solution treatments, namely (1) deionized water (DI) only (without any plant nutrition added), (2) complete solution (Hoagland’s complete nutrient solution), and (3) Ca deficiency solution. The results indicate that the ‘Orange Juice’ tulip was particularly susceptible to Ca deficiency, exhibiting stem toppling and flower abortion similar to those grown in DI. Ca deficiency led to a reduction in root length and leaf area but did not affect the plant height. Additionally, it had a negative impact on bulb qualities, resulting in a decreased bulb fresh weight and bulb circumference in both tulip cultivars. Furthermore, Ca deficiency and DI reduced the photosynthetic rates, and Ca deficiency increased the transpiration rate in the tulip. The leaf concentrations of nitrogen (N), phosphorus (P), and Ca were decreased under Ca deficiency and DI, while magnesium (Mg) levels increased under Ca deficiency treatment compared to the complete nutrient solution treatment.

Need for external replication trials in homeopathic basic research: presenting research on three duckweed test systems as an example

One of the main aims in homeopathic basic research is to find reliable test systems that are externally replicable. In face of the replication crisis in research in general and the specificities that are necessary to include in homeopathic basic research, it is of great importance to encourage high-quality replication trials. One way to achieve that is to build up a register or a data base for test systems in homeopathic basic research that are already developed to the point that external replication trials are possible. Such a register offers the opportunity to include not just the published information but also helpful insight such as material lists and time frames for easier planning and conduction of replication trials. Additionally, further information on critical environmental parameter, unpublished negative results, etc. could be included.
 The general frame work of such a register/ data base is demonstrated with the example of three different duckweed test systems developed in our working group since 2001. The growth rate of duckweed (Lemna gibba L., clone number 9352) was investigated in randomised and blinded settings, using either untreated plants or plants pre-treated with a stressor (arsenic solution or calcium deficiency in the growth medium). Within the original trials, potentised substances in the range of 14x-33x were compared to water control groups (succussed and unsuccussed water). The stability of the test systems was investigated in parallel systematic negative control (SNC) experiments. All original trials revealed a significant influence of some potencies on the growth rate of duckweed and stable test systems as shown with SNC experiments. Four internal replication trials for two of the three test systems were conducted. The replication trials showed effects of homeopathic preparations with some differences in effect size and effect direction compared to original studies.

Calcium and vitamin D homoeostasis in male fertility.

Calcium and vitamin D have well-established roles in maintaining calcium balance and bone health. Decades of research in human subjects and animals have revealed that calcium and vitamin D also have effects on many other organs including male reproductive organs. The presence of calcium-sensing receptor, vitamin D receptor, vitamin D activating and inactivating enzymes and calcium channels in the testes, male reproductive tract and human spermatozoa suggests that vitamin D and calcium may modify male reproductive function. Functional animal models have shown that vitamin D deficiency in male rodents leads to a decrease in successful mating and fewer pregnancies, often caused by impaired sperm motility and poor sperm morphology. Human studies have to a lesser extent validated these findings; however, newer studies suggest a positive effect of vitamin D supplementation on semen quality in cases with vitamin D deficiency, which highlights the need for initiatives to prevent vitamin D deficiency. Calcium channels in male reproductive organs and spermatozoa contribute to the regulation of sperm motility and capacitation, both essential for successful fertilisation, which supports a need to avoid calcium deficiency. Studies have demonstrated that vitamin D, as a regulator of calcium homoeostasis, influences calcium influx in the testis and spermatozoa. Emerging evidence suggests a potential link between vitamin D deficiency and male infertility, although further investigation is needed to establish a definitive causal relationship. Understanding the interplay between vitamin D, calcium and male reproductive health may open new avenues for improving fertility outcomes in men.

Assessment of risk factors for osteopenia development in premature babies

Neonatal osteopenia (KB 61.3 (ICD-11)) – violation of mineral exchange of bone tissue (BT) of premature babies (PB). Predicting and detection of osteopenia is the key to its successful control.
 Aim. The aim of the article was to determine risk factors influencing the formation of neonatal osteopenia of PB and during the term of intensive care / postintensive nursing.
 Materials and methods. Ultrasound densitometer Sunlight Omnisense 9000 was used to measure the ultrasound speed in BT (SOS, m/s) with estimation Z-score (SD) according to gestational age of 56 PB and 20 mature babies (control group) in the early neonatal age. Verification of osteopenia was carried out under the WHO guidelines for radiation methods by Z-score indicator less than SD by -1.0. Estimation of Z-score -2.0 SD and less was attributed to ultra-low indicators. The characteristics of obstetric and gynecological, somatic anamnesis and pregnancy course of premature babies’ mothers, factors of PB postnatal development that influence the formation of BT were studied.
 Results. Children born at 33 weeks or earlier are 3.23 times more likely (OR = 3.23; CI 95 % [1.08; 9.70]) to develop BT demineralization by the corresponding term of birth than other PB. The chances of ultra-low SOS by Z-score are 14.22 times higher (OR = 14.22; CI 95 % [3.29; 61.57]) in PB born at 32 weeks of gestation and earlier. Extragenital diseases, clinical signs of calcium deficiency in mothers, and women’s intake of calcium and vitamin D3 during pregnancy did not have a statistically significant difference (p > 0.05) in the studied PB and control group. The mother’s preeclampsia increases the chances of insufficient BT mineralization by 5.47 times (OR = 5.47; CI 95 % [1.07; 27.93]), second parity pregnancy (and subsequent ones) – by 4.51 times (OR = 4.51, CI 95 % [1.38; 14.80]). The factors of the ratio of the duration of mechanical ventilation relative to the total time of PB treatment, the duration of parenteral feeding have an inverse correlation (moderate (r = -0.42, р < 0.05) and significant (r = -0.51, р < 0.05) respectively) with a decrease in SOS to low Z-score.
 Conclusions. In PB born at 32 weeks of gestation and earlier, the chances of ultra-low SOS indicators by Z-score are 14.22 times higher (OR = 14.22; CI 95 % [3.29; 61.57]). The following factors predicts insufficient mineralization of BT according to SOS indicators according to the Z-score: mother’s second parity pregnancy (and subsequent ones), preeclampsia, parenteral feeding of PB for more than 8 days (Se = 75.00 %, Sp = 71.87 %, p < 0.0001), duration of mechanical ventilation, which is more than 6.48 % of the total time of PB treatment (Se = 86.67 %, Sp = 59.38 %, p < 0.046).

Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line.

Non-alcoholic fatty liver disease or steatosis is an accumulation of fat in the liver. Increased amounts of non-esterified fatty acids, calcium deficiency, or insulin resistance may disturb endoplasmic reticulum (ER) homeostasis, which leads to the abnormal accumulation of misfolded proteins, activating the unfolded protein response. The ER is the primary location site for chaperones like thioredoxin domain-containing 5 (TXNDC5). Glutathione participates in cellular oxidative stress, and its interaction with TXNDC5 in the ER may decrease the disulfide bonds of this protein. In addition, glutathione is utilized by glutathione peroxidases to inactivate oxidized lipids. To characterize proteins interacting with TXNDC5, immunoprecipitation and liquid chromatography-mass spectrometry were used. Lipid peroxidation, reduced glutathione, inducible phospholipase A2 (iPLA2) and hepatic transcriptome were assessed in the AML12 and TXNDC5-deficient AML12 cell lines. The results showed that HSPA9 and PRDX6 interact with TXNDC5 in AML12 cells. In addition, TXNDC5 deficiency reduced the protein levels of PRDX6 and HSPA9 in AML12. Moreover, lipid peroxidation, glutathione and iPLA2 activities were significantly decreased in TXNDC5-deficient cells, and to find the cause of the PRDX6 protein reduction, proteasome suppression revealed no considerable effect on it. Finally, hepatic transcripts connected to PRDX6 and HSPA9 indicated an increase in the Dnaja3, Mfn2 and Prdx5 and a decrease in Npm1, Oplah, Gstp3, Gstm6, Gstt1, Serpina1a, Serpina1b, Serpina3m, Hsp90aa1 and Rps14 mRNA levels in AML12 KO cells. In conclusion, the lipid peroxidation system and glutathione mechanism in AML12 cells may be disrupted by the absence of TXNDC5, a novel protein-protein interacting partner of PRDX6 and HSPA9.

A comparison of the bioaccessible calcium supplies of various plant-based products relative to bovine milk

Calcium deficiency is widespread globally, especially in diets with minimal consumption of dairy. It is therefore important to identify plant-based sources of calcium that can make a meaningful contribution to calcium intakes for populations following diets with a minimum supply of dairy products. The best sources of calcium have a high calcium content and bioavailability. Therefore, we evaluated the gross and bioaccessible calcium supplies of 25 plant-based products from 5 food groups considered to be good and important sources of calcium. Bioaccessible calcium was examined using the INFOGEST static digestion model in which isotopically labelled 43Ca was used as a tracer of reagent calcium to improve accuracy of bioaccessibility measurements. The gross calcium content varied widely amongst all the food products, ranging between 7.48 and 959 mg/100 g fresh weight (fw), with approximately 50 % of the products being equivalent to or surpassing the calcium content of skimmed milk. Bioaccessibility of calcium was equally variable, ranging from about 0.1 – 50 %. The lowest bioaccessibility (<10 %) was found in spinach, plant-based beverages, tofu, dried figs and tahini and was attributed to: 1. the high content of oxalate and phytate in some of the products, and 2. the low solubility of tricalcium phosphate which was used for fortification in the plant-based beverages. The remaining products generally had a high bioaccessibility that was similar to, or higher than that of skimmed milk (∼30 %). When both bioaccessibility and recommended serving portions were considered, only 3 products were identified as good sources of calcium, requiring 0.2 – 1.4 servings to equal the bioaccessible supply from skimmed milk. The top three sources of plant-based calcium identified were kale, finger millet and fortified white bread in that order, with kale providing 5 times more bioaccessible calcium than 1 serving of skimmed milk. Moderate sources of calcium where 1.5 – 3 servings was equivalent to 1 serving of skimmed milk included wholemeal bread, some bean varieties (black chickpeas, chickpeas, kidney beans, peas), broccoli, cabbage and almond drink. The rest of the products were either of low calcium content, poor bioaccessibility, and/or not consumed in sufficient quantities to make a significant contribution to daily requirements. White bread was a good source of calcium as it was fortified with calcium carbonate and this suggests that mandatory widescale fortification of staple cereals with this form of calcium should be considered a viable approach to augment dietary calcium intakes in vulnerable populations. Low bioaccessibility of fortified calcium in plant-based beverages, often marketed as good sources of calcium, suggests the need for regulation and for further in vivo studies to validate bioavailability of calcium in these products.

Comparison of lifestyle and nutritional status between women with and without metabolic syndrome.

Amidst the growing epidemic of obesity and diabetes, metabolic syndrome (MetS) is one of the greatest health problems. Lifestyle factors and bad habits are responsible for the emergence and development of MetS. The aim of the study was to compare and check whether women with MetS adhered to a healthy diet and had a healthier lifestyle than women without MetS, and to compare the impact of women's lifestyles on their nutritional status. The study included 167 women aged 20-78. As per guidelines, all tests required fasting. Comparisons of individual quantitative variables between groups were made using the Student's t-test for independent variables or the Mann-Whitney U-test. The relationship between quantitative variables was verified with the Spearman correlation coefficient. All statistical tests were based on a significance level of p<0.05. Women with MetS were more likely to have bad habits such as smoking, adding sugar to beverages and adding salt to food on the plate, while alcohol consumption was more common in women without MetS, but there was a statistically significant positive correlation between alcohol consumption and HDL-cholesterol levels in both groups. Women with MetS showed statistically significantly higher body weight, BMI, waist and hip circumferences (p<0.001), a statistically significant positive relationship between adding sugar to beverages and triglyceride levels (r=0.2699, p=0.015). In women without MetS, there was a statistically significant negative correlation between HDL-cholesterol levels and cigarette smoking (r= 0.2709, p=0.014). Women with MetS had statistically significantly higher mean systolic and diastolic blood pressure and mean serum levels of total cholesterol (p<0.001), LDL-cholesterol (p=0.007), triglycerides (p<0.001) and glucose (p<0.001) than women without MetS. Nutritional errors were shown in both groups, including deficiencies in calcium, folates, iron, vitamin D and excessive sodium and phosphorus intake. Therefore, it is important to prevent the onset of MetS components and to educate and assist professionals in setting realistic goals individually adapted to each patient.

Effect of Vitamin D and Calcium Supplementation on Insulin Resistance

As people spend more time indoors, Vitamin D deficiencies have increased drastically due to less sunlight exposure. New studies have linked Vitamin D deficiencies to calcium deficiencies since Vitamin D helps the human body absorb calcium properly. Calcium regulates insulin sensitivity, and calcium deficiencies reduce insulin sensitivity, indicating how Vitamin D deficiencies can lead to insulin resistance. I tested the individual and combined effects of calcium and Vitamin D supplementation on insulin resistance in Drosophila melanogaster, using physical activity to measure improvement in insulin resistance. There were 3 identical trials, and 5 vials in each trial. The control group was fed with only drosophila medium. The remaining vials had 3 grams of table sugar mixed with the medium. The second vial had no additions. The third vial had an addition of liquid calcium, and the fourth vial had Vitamin D instead. The last group had both supplements. After a week, the peristalsis contractions of each group’s larvae were counted in 30-second intervals. Additionally, adult flies from different groups were used for a climbing assay to measure the short-term impacts of these supplements on physical activity. Both measures yielded similar results: there was a visible increase in climbing performance and the number of peristalsis contractions in vials with supplementations compared to those without. The groups fed with individual supplements saw some increase, but the group with both supplements saw the most significant increase relative to sugar supplementation alone, supporting the hypothesis that calcium and Vitamin D supplementation improves insulin resistance.

Nutrition Adequacy Level of Pregnant Women and Toddlers Aged 0 to 24 Months in Gianyar Regency

Stunting has become a national issue that has received a lot of attention recently, as stunting can affect the nation's productivity in the future. The adequacy of nutritional intake in months 0 up to 24 months, or 1000 days of life, is one of the key factors in preventing stunting. This study aims to determine the nutrition adequacy level of pregnant women and children aged 0 to 24 months in Gianyar Regency. This study was a household survey with a cross-sectional approach. The samples were households in Gianyar Regency in 2021 with pregnant women and young children (up to 24 months old), chosen using the cluster method with the census block serving as a cluster. From each selected census block, 5 samples of pregnant women, 5 samples of children aged 0–1 year, and 5 toddlers aged 1-2 years will be selected by a simple random method. Data was collected using the Semi-Quantitative Food Frequency Questionnaire (SQ-FFQ). Data analysis was performed using the NutriSurvey application. The total energy of pregnant women was moderately deficient; protein and fat adequacy were normal, while carbohydrates, calcium, and iron were severely deficient. Nutritional adequacy for toddlers aged 0–1 year in the form of energy, protein, fat, carbohydrates, calcium, and iron was classified as normal. Nutritional adequacy for toddlers aged 1-2 years in the form of energy, protein, fat, calcium, and iron was classified as normal, while carbohydrates were moderately deficient. The presence of nutrition deficiencies among pregnant women indicates that a public health program regarding carbohydrate, calcium, and iron deficiencies is urgently needed.

Sheep bone valorization: Enhancing gastronomic sustainability through hydroxyapatite-enriched potato wedges

In pursuit of sustainability and resource optimization, the zero-waste theory has gained momentum across diverse sectors. The concept of "zero waste" integrates waste management strategies to minimize waste such as animal bones. This study aimed to prepare Calcined and Nano hydroxyapatite (HAp) from sheep bone as a valuable calcium source by using calcination (850 °C) and ultra-sonication methods. FTIR, particle size distribution (PSD), atomic absorption spectroscopy (AAS), laser-induced breakdown spectroscopy (LIBS), scanning transmission electron microscopy (STEM), and XRD techniques were used to characterize the resulting samples. FTIR spectra confirmed PO43− at 1027.43 and 1030.32 cm−1 in nano and calcined HAp respectively. AAS, UV, and PSD indicated 32.3 ± 0.11% calcium content and 18.86 ± 0.19% P, with 142 nm particle size in Nano-HAp; which significantly differed from calcined HAp. STEM confirmed morphological behavior. XRD crystalline behavior seemed highest at 460a.u., whereas LIBS-derived Ca/P ratio was 1.99 in Nano HAp. Notably, in-vitro findings measured the highest bioavailable calcium content in Nano HAp (46.6%) and Calcined HAp (42.1%). Further, HAp was used to develop and characterize fortified baked potato wedges with formulations; 0%, 0.22%, 0.44%, and 0.66%. Moisture (%) was measured within the range of 21.51 ± 0.11 to 34.83 ± 0.03. Nano-Bio-2 (0.44%) was the best formulation that could be a better choice to wipe out calcium deficiency for end-users. Moreover, principal component analysis (PCA) was applied to the physical and sensory attributes of the developed product. On account of eigenvalues, the total variation was recorded at 94.71. Hence, current work showcased the potential of utilizing sheep bone for sustainable calcium enrichment and offered insights into innovative food formulations.

Normocalcemic hyperparathyroidism: Intervention to differentiate primary from secondary hyperparathyroidism

BackgroundNormocalcemic hyperparathyroidism can occur, but surgery should not be considered until common etiologies for secondary hyperparathyroidism are comprehensively excluded. Calcium deficiency is an underrecognized cause of normocalcemic parathyroid hormone elevation, and we aim to determine if the implementation of a preoperative calcium challenge can be used to reduce unnecessary parathyroidectomy. MethodsConsecutive patients referred for parathyroidectomy (1/21–6/22) with normocalcemia (serum calcium <10 mg/dL) and concurrently elevated parathyroid hormone levels were routinely treated with supplemental calcium and vitamin D3, and follow-up laboratory studies were assessed. ResultsA total of 29/314 (9%) patients had normocalcemic parathyroid hormone elevation with mean calcium, parathyroid hormone, and vitamin D 25OH levels of 9.5 ± 0.3 mg/dL, 109.9 ± 34.9 pg/mL, and 42.7 ± 23.8 ng/mL respectively. Confounding factors included estimated glomerular filtration rate <60 in 2, loop diuretic use in 4, and prior gastric bypass or gastric sleeve surgery in 4. Follow-up biochemical evaluation was available in 27 (92%); results were unchanged in 7 patients (26%); normalization of parathyroid hormone levels with persistently normal calcium levels occurred in 15 (55%), thus confirming secondary hyperparathyroidism and hypercalcemia with elevated parathyroid hormone levels (classic primary hyperparathyroidism) was diagnosed in 5 (19%). Parathyroid exploration has been completed for 3 of 5 patients with classic primary hyperparathyroidism to date. ConclusionA preoperative calcium challenge was prospectively initiated in normocalcemic patients with parathyroid hormone elevation, and there was high compliance (92%). Short-interval calcium supplementation revealed ∼50% to have resolved secondary hyperparathyroidism due to insufficient calcium intake, which avoided unnecessary surgery. In contrast, classic patients were unveiled in 20%, allowing for prompt and correct surgical intervention.

Vascular Tissues Distribution Affects Calcium and Calcium Oxalate Crystals in Fruits of Wild Tomato (Lycopersicon pimpinellifolium (L.) Mill.).

Tomato fruit is an excellent model for evaluating calcium regulation in plants since it expresses symptoms of either calcium deficiency or calcium excess. Aiming to evaluate the structure of the vascular system and its interactions with calcium and calcium oxalate crystals (CaOx), fruits of Lycopersicon pimpinellifolium were studied. Calcium levels were evaluated in basal, median, and distal pericarp portions, which were also analyzed under a light microscope to describe the structure. The L. pimpinellifolium pericarp shows idioblasts with calcium oxalate crystals. Vascular bundles of the basal pericarp show large transverse sections and abundant xylem vessels. The vascular bundles were smaller in the distal pericarp, and the xylem showed fewer and narrower vessels. The terminal bundles often consisted exclusively of phloem. Despite the differences observed in vascular bundle composition, the density of the vascular system was uniform in the pericarp as a consequence of bundle ramifications that occur at distal portions. The calcium concentration and crystal idioblasts decrease towards the apex of the fruit. The reduction in the xylem:phloem ratio seems to determine the low calcium concentration in the distal fruit portion.

Some indicators of nutritional security of children with restrictive types of nutrition

Following vegetarian diets means, to varying degrees, the exclusion of animal products from diets, which, if poorly planned, can lead to deficiency of some nutrients. Aim of the study was to evaluate some indicators of nutritional security of children with a restrictive type of nutrition and living in the territory of a long-term military conflict in the Donbass. Material and methods. 80 school-age children following restrictive diets were examined. All patients were divided into two groups: 40 children following a dairy-free diet (group 1), 40 children on a vegetarian type of diet (group 2). The control group consisted of 30 children who adhere to the traditional type of diet. Calcium, ferritin, vitamin B12 and zinc content in blood serum were studied in all children, living in Donbass. Results. In group 1 deficiency of calcium was found in 60.0 %, of zinc – in 32.5 %, of vitamin B12 – in 15.0 %, a decrease in ferritin content – in 22.5 %. In group 2 level of ferritin was declined in 55.0%, zinc deficiency was detected in 37.5 %, vitamin B12 deficiency – in 30.0 %, calcium deficiency – in 10.0 %. Conclusions. For children on a long-term dairy-free diet, calcium and zinc deficiency is characteristic, as well as a decrease in tissue iron reserves. Children who observe lactovegetarianism are characterized by a deficiency of zinc and vitamin B12, as well as a decrease in the concentration of ferritin in the blood serum. Children living in the Donbass in the conditions of a long military conflict and adhering to restrictive types of nutrition can be considered as a risk group for the development of deficient conditions. To correct the identified imbalance, it is necessary to develop programs for the supplementation of macro- and micronutrients.

Stanniocalcin 1a regulates organismal calcium balance and survival by suppressing Trpv6 expression and inhibiting IGF signaling in zebrafish.

Stanniocalcin 1 (Stc1) is well known for its role in regulating calcium uptake in fish by acting on ionocytes or NaR cells. A hallmark of NaR cells is the expression of Trpv6, a constitutively open calcium channel. Recent studies in zebrafish suggest that genetical deletion of Stc1a and Trpv6 individually both increases IGF signaling and NaR cell proliferation. While trpv6-/- fish suffered from calcium deficiency and died prematurely, stc1a-/- fish had elevated body calcium levels but also died prematurely. The relationship between Stc1a, Trpv6, and IGF signaling in regulating calcium homeostasis and organismal survival is unclear. Here we report that loss of Stc1a increases Trpv6 expression in NaR cells in an IGF signaling-dependent manner. Treatment with CdCl2, a Trpv6 inhibitor, reduced NaR cell number in stc1a -/- fish to the sibling levels. Genetic and biochemical analysis results suggest that Stc1a and Trpv6 regulate NaR cell proliferation via the same IGF pathway. Alizarin red staining detected abnormal calcium deposits in the yolk sac region and kidney stone-like structures in stc1a -/- fish. Double knockout or pharmacological inhibition of Trpv6 alleviated these phenotypes, suggesting that Stc1a inhibit epithelial Ca2+ uptake by regulating Trpv6 expression and activity. stc1a-/- mutant fish developed cardiac edema, body swelling, and died prematurely. Treatment of stc1a-/- fish with CdCl2 or double knockout of Trpv6 alleviated these phenotypes. These results provide evidence that Stc1a regulates calcium homeostasis and organismal survival by suppressing Trpv6 expression and inhibiting IGF signaling in ionocytes.

Assessment of calcium intake and recognition of calcium-rich food sources among the Saudi population.

Calcium is an essential mineral and one of the most prevalent in the body. Chronic insufficient calcium intake increases the risk of osteopenia, osteoporosis, and bone fracture. This study aimed to assess the Saudi population's calcium intake and knowledge of calcium sources as well as to identify factors associated with inadequate calcium intake. This cross-sectional study was conducted across Saudi Arabia using an online questionnaire distributed randomly through social media channels. The questionnaire was a validated tool that was previously developed and used by the original author to assess knowledge of calcium food sources and estimate calcium intake in the Saudi population. If an individual answered more than 11 questions correctly, calcium knowledge was considered adequate. Calcium intake was considered sufficient or insufficient based on the recommended dietary allowance (RDA) of 1000 mg/day. The survey included male and female Saudi citizens and residents aged 14 years or older. A total of 950 respondents aged 9-70 completed the questionnaire. A total of 62.9% of the respondents were 26-50 years old. Of the participants, 64.2% were female, 71.7% were married, and 61.9% had a diploma or a bachelor's degree. Of these, 63.8% were from the central region, and 97.6% were from Saudi Arabia. For those aged 9-18, the average calcium consumption was 577 mg/day, whereas it was 479 mg/day for those aged 19-50. The average intake was 479 mg/day for males aged 51-70 (EAR = 1000 mg/day) and 438 mg/day for females (EAR = 1200 mg/day). These calcium consumption values were significantly lower than the estimated average requirement (EAR) and RDA (p < 0.01). Correct response rates for the 19 calcium intake knowledge items ranged from 23.4 to 94.7%. Among the participants, 48.9% had significant calcium intake knowledge, as indicated by a score of > 11 out of 19 correct responses. Age, sex, marital status, educational attainment, and residence were significantly correlated with adequate knowledge of calcium. Demographic characteristics such as age, marital status, and residence were associated with sufficient calcium intake in the study. The study findings revealed that a significant proportion (51.1%) of participants demonstrated inadequate knowledge regarding calcium food sources. Additionally, the mean calcium intake was found to be lower than the RDA of 1300 mg/day for males and females 14-18 years old and 1000 mg/day for males and females 19-50 years old and males 51-70 years old; however, the RDA for females 51-70 years old is 1200 mg/day, suggesting an urgent need for interventions aimed at enhancing both calcium knowledge and intake.

Bacterial or fungal origin phytase enzyme affects the performance and mineralization of calcium and phosphorus differently in broiler chickens fed deficient calcium and phosphorous diets

ABSTRACT Three Ca-P deficient diets viz., Diet-1 (0.85%, 0.35%), Diet-2 (0.75%, 0.30%) and Diet-3 (0.65%, 0.25%) were prepared and supplemented with either bacterial or fungal origin phytase enzymes and were compared with a control diet (1.0% and 0.45%). A total of 280 broiler (Ross 308) chicks were randomly allocated into 7 treatments having 4 replicates of 10 chicks using a completely randomized design. The effect of bacterial phytase on the growth, FCR and carcass weight was significantly (P < 0.05) higher in chicks on Diet-1 compared to diet-3 (28 days). Bacterial phytase increased serum minerals (Ca and P) and tibia ash in diet-1 compared to Diet-3. Tibia Ca was significantly (P < 0.05) higher in the control and the three diets under bacterial phytase compared to other treatments. On Diet-3, more Ca and P were reflected in tibial by bacterial phytase but did not support the optimum growth performance of broiler chicks. In conclusion, the same phytase but of different origins might have specific individual effects on the growth performance, serum and tibia Ca and P in broilers.

Change in Mineral Status After Bariatric Surgery: a Meta-analysis.

The risk of protein and vitamin deficiencies after bariatric surgery has been well studied, but the change in mineral status has not gotten enough attention. This study aimed to perform a meta-analysis regarding the change in mineral levels after bariatric surgery and the prevalence of postoperative mineral deficiency, with subgroup analyses of different surgical procedures, study regions, and follow-up time. CENTRAL, PubMed, and EMBASE were searched for related articles. Meta-analysis, subgroup analysis, and sensitivity analysis were performed if necessary. A total of 107 articles with 47,432 patients were included. The most severe mineral deficiency after bariatric surgery was iron (20.1%), followed by zinc (18.3%), copper (14.4%), chlorine (12.2%), phosphorus (7.5%), and calcium (7.4%). Serum concentrations of potassium, sodium, selenium, manganese, and molybdenum showed no significant change before and after surgery. Subgroup analyses revealed that SG had fewer deficiencies in serum iron, calcium, zinc, magnesium, phosphorus, copper, and selenium than RYGB. OAGB showed a higher incidence of serum iron and zinc deficiencies than RYGB. Studies conducted in different regions also found various mineral statuses after surgery. Studies with follow-up ≥ 5 years had a lower prevalence of zinc, copper, and selenium deficiencies than follow-up < 5 years. A high deficiency rate of serum iron, zinc, copper, chlorine, phosphorus, and calcium was seen after bariatric surgery. The difference in surgical procedures, study regions, and follow-up time may affect postoperative mineral status; more targeted mineral supplement programs are needed considering these influencing factors.

Infantile tremor syndrome: a case report

Infantile tremor syndrome (ITS) is a rare but significant clinical condition characterized by a tetrad of pallor, developmental delay or regression, skin pigmentation abnormalities, and sparse brown scalp hair, often accompanied by involuntary tremors. ITS primarily affects children aged 5 months to 3 years, with a higher prevalence in males, especially in regions like the Indian subcontinent, Southeast Asia, and Africa. The exact cause of ITS remains uncertain, but a strong association with vitamin B12 deficiency has been observed in numerous studies. Malnutrition, including deficiencies of zinc, magnesium, iron, calcium, and hypoalbuminemia, is common among affected infants, who often come from lower socioeconomic backgrounds with vegetarian mothers lacking animal food in their diets. In this case report, an 8-month-old female child presented with progressive tremors, poor feeding, and developmental delay. The child had been exclusively breastfed by a vegetarian mother and lacked proper complementary feeding. Clinical examination revealed pallor, hypopigmented hair, hyperpigmented knuckles, and generalized hypotonia. Laboratory tests confirmed vitamin B12 deficiency, anemia and other nutritional deficiencies. Neuroimaging showed cerebral atrophy. Treatment involved addressing the nutritional deficiencies, primarily through vitamin B12 supplementation, iron, magnesium, calcium, and a protein-rich diet. Tremors were managed with propranolol and phenobarbitone. Within a month of treatment, the child exhibited significant improvement in tremors, developmental milestones, and overall well-being. This case emphasizes the importance of considering ITS in infants with specific clinical features and highlights the role of healthcare providers in educating parents about proper nutrition during pregnancy and infancy. Early diagnosis and management, including nutritional interventions and symptom control, can lead to substantial improvements in affected children's health and development. Further research is necessary to better understand the etiology and optimal management strategies for ITS.

Профілактика порушень у слизових оболонках травного тракту самок щурів за дефіциту естрогенів, білка та кальцію

Purpose. Substantiation of the preventive efficacy of a complex consisting of quercetin, calcium citrate, vitamins, macro- and microelements in relation to the biochemical parameters of the mucous membranes of the digestive tract of rats with oophorectomy and insufficient protein and calcium in the diet. Materials and methods. The study was conducted on 24 female rats of herd breeding: in 16 animals, the state of estrogen deficiency was modeled by ovariectomy followed by their maintenance on an inadequate diet, half of which were injected with a prophylactic complex of vitamins and minerals. After 4 months, the rats were taken out of the experiment. In the homogenates of the mucous membranes of the oral cavity, stomach, small and large intestine, markers of inflammation (activity of elastase, acid phosphatase and the content of malonic dialdehyde) and the index of bacterial contamination (activity of urease) were determined. Results. As a result of the study, the development of inflammation was established in the mucous membranes of the digestive tract of rats with hypoestrogenism and when receiving a diet deficient in protein and calcium. The development of inflammation was accompanied by an increase in the activity of acid phosphatase (in the oral cavity – by 16.5 %, in the stomach – by 23.3 %, in the small intestine – by 27.0 %, in the large intestine – by 29.6 %), elastase ( in the oral cavity – by 9.2 %, in the stomach – by 52.6 %, in the small intestine – by 93.6 %, in the large intestine – by 28.7 % and the content of malondialdehyde (in the oral cavity – by 25.8 %, in the stomach – by 40.7 %, in the small and large intestine – by 26.4 %, in the large intestine – by 35.7 %) relative to the control group. In addition, an increase in urease activity was registered in the mucous membranes of the digestive tract of rats with pathology, which means an increase in the contamination of opportunistic microorganisms on the mucous membranes. The introduction of the prophylactic complex to rats prevented an increase in inflammation and dysbiosis in the mucous membranes of the digestive tract of animals, which was caused by pathology modeling. Findings. Anti-inflammatory and anti-dysbiotic properties of the prophylactic complex of vitamins and minerals based on quercetin and calcium citrate from Black Sea oyster shells allow us to recommend this composition of preparations for a deeper study in order to create an alternative to hormone replacement therapy for hypoestrogenism with alimentary deficiency of protein and calcium.