Sappan Research Articles

Exploring the Mechanism of Ferroptosis Induction by Sappanone A in Cancer: Insights into the Mitochondrial Dysfunction Mediated by NRF2/xCT/GPX4 Axis.

Non-small cell lung cancer (NSCLC), a major subtype of lung cancer, encompasses squamous cell carcinoma, adenocarcinoma, and large cell carcinoma. Compared to small cell lung cancer, NSCLC cells grow and divide more slowly, and their metastasis occurs at a later stage. Currently, chemotherapy is the primary treatment for this disease. Sappanone A (SA) is a flavonoid compound extracted from the plant Caesalpinia sappan, known for its antitumor, redox-regulating, and anti-inflammatory properties. Recent studies have investigated the interaction of SA with mitochondrial pathways in regulating cell death through the Nrf-2/GPX-4/xCT axis. This study specifically explores the mechanism by which SA affects mitochondrial morphology and structure through the regulation of mitophagy and mitochondrial biogenesis in tumor cells. The study primarily utilizes second-generation transcriptomic sequencing data and molecular docking techniques to elucidate the role of SA in regulating programmed cell death in tumor cells. The omics results indicate that SA treatment significantly targets genes involved in oxidative phosphorylation, mitophagy, mitochondrial dynamics, and oxidative stress. Further findings confirmed that the Nrf-2/GPX4/xCT pathway serves as a crucial target of SA in the treatment of NSCLC. Knockdown of Nrf-2 (si-Nrf-2) and Nrf-2 overexpression (ad-Nrf-2) were shown to modulate the therapeutic efficacy of SA to varying degrees. Additionally, modifications to the GPX4/xCT genes significantly affected the regulatory effects of SA on mitochondrial autophagy, biogenesis, and energy metabolism. These regulatory mechanisms may be mediated through the caspase pathway and ferroptosis-related signaling. Molecular biology experiments have demonstrated that SA intervention further inhibits the phosphorylation of FUNDC1 at Tyr18 and downregulates TOM20 expression. SA treatment was found to reduce the expression of PGC1α, Nrf-1, and Tfam, resulting in a decrease in mitochondrial respiration and energy metabolism. Overexpression of Nrf-2 was shown to counteract the regulatory effects of SA on mitophagy and mitochondrial biogenesis. Confocal microscopy experiments further revealed that SA treatment increases mitochondrial fragmentation, subsequently inducing mitochondrial pathway-mediated programmed cell death. However, genetic modification of the Nrf-2/GPX4/xCT pathway significantly altered the regulatory effects of SA on tumor cells. In conclusion, SA has been identified as a promising therapeutic agent for NSCLC. The mitochondrial pathway-mediated apoptosis and ferroptosis may represent key mechanisms in regulating tumor cell death. Targeting the Nrf-2/GPX-4/xCT axis offers a novel therapeutic approach for maintaining mitochondrial homeostasis within the cellular microenvironment.

Pharmaceutical activity of sappan wood extract (Caesalpinia sappan L.) for treating Escherichia coli infection in piglets.

Escherichia coli infection is one of the major diarrheal diseases resulting in the loss of pigs at a young age. This research investigated the antimicrobial activity of Caesalpinia sappan wood extract against E. coli infection as an antibiotic replacement. E. coli was cultured from diarrheal piglets and then used to find the minimal inhibition concentration (MIC). Caesalpinia sappan wood extract (500 mg/kg) was used for the treatment of diarrheal piglets compared to antibiotics (enrofloxacin 5 mg/kg) by oral administration. Another three groups of diarrheal piglets were used supplemented feed with 1% and 2% extract compared with commercial feed. Subsequently, E. coli enumeration, fecal shape, fecal color, and growth rate were recorded from day 1 to 7. Based on the results, C. sappan wood extract could inhibit E. coli growth at a MIC of 16-34 mg/ml. The number of colonies did not significantly differ between C. sappan wood extract and enrofloxacin treatment groups. A supplemented feed with 1% and 2% C. sappan wood extract could improve the fecal shape and fecal score compared to the control group, albeit only in suckling pigs. There were significant differences from the control group on days 4, 5, 6, and 7 (p < 0.05). However, the average daily gain did not significantly differ among the three groups. The results indicate that C. sappan wood extract could improve diarrheal signs in suckling pigs and can be used as a replacement for antibiotics for organic pig production.

Anti-inflammatory activity of sappan wood extract cream (Caesalpinia sappan) in porphyromonas gingivalis-induced gingivitis rats models

Background: Gingivitis is a dysbiotic condition characterized by persistent inflammation caused by a disease-associated multispecies bacterial population that has established itself in the subgingival region. Aim: The objectives of this study is to analyze the anti-inflammatory activity of sappan wood extract cream (SWC) in Porphyromonas gingivalis induced-periodontitis rats model. Methods: In this study, rats were infected to the gingiva with P. gingivalis as an inducer for gingivitis rats model for 14 days. The SWC were treated topically into the infected tissue. The inflammation and angiogenesis of gingival tissue was analyzed using histological examination. An immunohistochemistry (IHC) assay was used to analyze the Nuclear Factor kappa-B (NF-kB) protein expression. Masson Trichrome (MT) assay was used to analyze the histopathology of collagen Scores. The Tumor Necrosis Factor-α (TNF-α), Interleukin-1 β (IL-1β), IL-6, and P38 genes expression were measured using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Results: The induction of P. gingivalis -induced the gingivitis in rats model indicated by reddish gingiva and bacterial plaque. MT tests showed that SWC increased collagen density, HE test showed increased angiogenesis and in contrast, lowered inflammation score. IHC test showed that SWC decreased the expression of NF-kB protein. The TNF-α, IL-1β, IL-6, and P38 gene expression were also decreased due to the SWC treatment. Conclusion: Sappan wood extract cream proves that it has anti-inflammatory activity which has the potential to prevent or treat gingivitis

Difference in organoleptic test results and antioxidant activity in variations of the method of making Emprit Ginger and Secang Wood powdered drink

Antioxidants in ginger (Zingiber officinale) and sappan wood play roles in health benefits, including dilating blood vessels and inhibiting cancer cells. The study analyzes the differences in organoleptic characteristics and antioxidant activity of powdered ginger and sappan wood, comparing Crystallization (MK) and conventional drying (MP) methods. In the crystallization method, 100 grams of Emprit Ginger and 5 grams of sappan wood sticks were blended, followed by adding 500 ml of water and 50 grams of sugar, then stirred in a hot pan for 2 hours until crystals formed. In the conventional drying method, an equal amount of ginger was dried for 5 days, and then, together with sappan wood and sugar, it was ground using a food processor. The data from the organoleptic test were normal, and the T-test result showed that powdered drinks from the drying method significantly exhibited better hedonic quality (p&lt;0,05). For every 100 grams, this powder contains 386 kcal of energy, 1,62% ash content, 2,18% moisture content, 91,88% carbohydrates, 1,0% fat, 2,32% protein, and 2040,86 mg/kg of antioxidant activity (IC50 method). In conclusion, different methods affect the characteristics of ginger powder, which has implications for product development and medicinal uses.Keywords:

ANTIVIRAL ACTIVITY OF FABACEAE AND ASTERACEAE FLAVONOID COMPOUNDS AGAINST SARS-CoV-2 ON SPIKE PROTEIN, MAIN PROTEASE (Mpro) AND RNA DEPENDENT RNA POLYMERASE (RdRp) RECEPTORS WITH IN SILICO METHOD

This study was conducted to analyze the antiviral activity of flavonoid compounds of Fabaceae (Clitoria ternatea, Caesalpinia sappan, Leucaena leucocephala, Indigofera tinctoria) and Asteraceae (Eclipta prostrata, Artemisia vulgaris, Blumea balsamifera, Chromolaena odorata) against SARS-CoV-2 on spike protein, Mpro and RdRp receptors by molecular docking method in silico. The results of molecular docking with AutoDock Vina showed that the flavonoid compounds semiglabrin (-7,5 kcal/mol) and pseudosemiglabrin (-7,0 kcal/mol) has binding energy better than native ligand NAG (-4,9 kcal/mol) and ceftriaxone (-6,8 kcal/mol) as a standard drug against the spike protein receptor. Flavonoid compounds juglanin (-8,5 kcal/mol) and glabratephrin (-8,5 kcal/mol) has binding energy close to the native ligand 4WI/nirmatrelvir (-8,7 kcal/mol) against Mpro receptor. Flavonoid juglanin (-9,5 kcal/mol) has binding energy better than native ligan 1RP/favipiravir (-8,3 kcal/mol) and remdesivir (-8,7 kcal/mol) as a standard drug, while flavonoid apigetrin (-8,7 kcal/mol) has binding energy better than native ligand and has the same binding energy with remdesivir as a standard drug against RdRp receptor.

Anti-Arthritic Activity of Combination of Caesalpinia sappan and Zingiber officinale Extracts in Complete Freund’s Adjuvant- Induced Arthritic in Rats

Anti-Arthritic Activity of Combination of Caesalpinia sappan and Zingiber officinale Extracts in Complete Freund’s Adjuvant- Induced Arthritic in Rats

Morphology Analysis of Hair Photoinduced and Chemical Damaged After Treatment with Sappan Wood (Caesalpinia sappan L.) Hair Tonic using SEM

Exposure to sunlight for a long time and repeatedly can also cause chemical and physical damage to human hair. Clinically, microscopic analysis using a scanning electron microscope (SEM) can assess hair damage by identifying the characteristic morphology of hair damage. This study aims to analyze the morphology of damaged hair chemically induced using 3% H2O2 and photoinduced UVB radiation before and after applying sappan wood (Caesalpinia sappan) hair tonic. The active ingredients used were ethanol extract, ethanol fraction, and chloroform-methanol fraction of C. sappan, which contains an antioxidant compound. Caesalpinia sappan simplicia was macerated using 96% ethanol and then partitioned using n-hexane. The ethanol fraction was then applied using vacuum column chromatography using chloroform : methanol (5 : 1) as eluent. The extracts and fractions were then formulated into hair tonic preparations. For SEM analysis, hair samples were coated with a sputter gold coater machine and divided into five treatments: undamaged hair, damaged hair with 3% H2O2 and UVB rays as a positive control, and treatment I, II, and III, in which the hair was damaged with 3% H2O2 and UVB rays respectively, then FI, FII, and FIII were applied, respectively (2000x magnification). Damaged hair with UVB induction shows moderate damage, and 3% H2O2 shows moderate to severe damage. The results in the treatment group show that the three hair tonics coated the hair cuticle, indicating an interaction with the hair fiber, and modified the cuticle by coating the cuticle.

WITHDRAWN: Ethyl acetate extracted from the chinese medicinal plant Caesalpinia sappan L alleviates gustatory response in diabetes mellitus rats.

Ahead of Print article withdrawn by publisher. This article has been withdrawn at the request of the author(s) they authors have found some mistakes that could affert the conclusion. The publisher apologizes for any inconvenience this may cause.

Physico-chemical Characteristics of Jelly Drink with Variation of Red Dragon Fruit Peel (Hylocereus polyrhizus) and Additional Sappan Wood (Caesalpinia sappan)

Red dragon fruit peel can be developed into a jelly drink because it naturally contains pectin which can be used as a thickening agent. Sappan wood contains bioactive substances including antioxidants of 15.69 ppm, anthocyanin 2.43%, and brazilin which can increase the red color in jelly drinks. This study contributes to determining the physicochemical characteristics of the red dragon fruit peel jelly drink with the addition of sappan wood. This study consisted of three formulations and a control. The concentrations of red dragon fruit peel and sappan wood were F1=1.5:0.5, F2=1.7:0.3, F3=1.9:0.1, and control (F4=2 :0). The product evaluation consists of pH, gel strength, viscosity, crude fiber, and moisture content. This study showed that the physical properties of jelly drink had a pH value between 6.49-6.67, a gel strength between 0.20-0.22, and a viscosity between 372-583 mPa.s. The chemical properties of jelly drinks have 1.29-2.13% crude fiber and 71.27-76.69% moisture content. This study showed that the higher the red dragon fruit peel concentration and the lower the sappan wood used, the higher the gel strength, viscosity, and crude fiber content, and lowered the pH value and moisture content.

Synthesis, Characterization and in-vitro Biological Studies of Curcumin decorated Biogenic Selenium Nanoparticles

Selenium nanoparticles have drawn interest recently, due to their distinctive physicochemical characteristics and potential usage in numerous applications, including medicine, electronics and catalysis. In this study, we employed Biancaea sappan (BS), often known as sappan wood or Brazilwood, which is a flowering tree that is endemic to Southeast Asia. Due to its antioxidant, anti-inflammatory and anticancer properties, this tree’s heartwood is frequently used in traditional medicine. Additionally, the wood is used to make natural dyes. In this study, we aim to develop an ecofriendly selenium nanoparticle using BS[Formula: see text] as a natural reducing agent and curcumin as a capping agent. The nanoparticles were synthesized using the green synthesis method and characterized using various techniques. Biocompatibility was evaluated using hemolytic assay and the bioactivity of the nanoparticles was assessed using anti-inflammatory and antioxidant assays. Selenium nanoparticles (BCSeN) were successfully synthesized using BS bark extract and functionalized with curcumin. The nanoparticles were characterized by UV-Vis Spectroscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy and Energy-dispersive X-ray spectroscopy (EDX) analysis, confirming their morphology, crystallinity, functionalization, elemental composition, size and stability. In vitro, bioactivity studies revealed that the BCSeN exhibited significant anti-inflammatory activity. They also demonstrated notable antioxidant efficacy against DPPH (2,2-diphenyl-1-picrylhydrazyl) and were also found to have minimal hemolytic potential. Our findings highlight the potential of BCSeN as a promising candidate for anti-inflammatory and antioxidant applications. However, further in-depth analysis is required to fully understand their efficacy and toxicity.

A novel approach for sterilization and concentration of traditional functional drinks: Membrane‐based nonthermal processes

AbstractThe applicability of microfiltration (MF) and osmotic membrane distillation (OMD) for sterilization and concentration of traditional functional drink constituents, such as Curcuma xanthorrhiza, ginger, lime, Java tea, and sappan wood extracts, was evaluated in this study. MF showed excellent performance in microorganisms elimination, turbidity reduction, and maintaining antihyperglycemic activity performance. However, a reduction of total phenol and antioxidant activity were observed due to the retention by MF. Fouling in MF was challenging and resulted in rapid flux decline. The concentration of sterilized extracts by OMD operation resulted in a concentration factor of 1.15, 1.13, 1.10, 1.24, and 1.24 for ginger, lime, C. xanthorrhiza, Java tea, and sappan wood, respectively. The active compounds were well preserved, with a maximum of 20% reduction compared to sterilized extracts. Despite insignificant foulant deposition, the OMD flux in tests using C. xanthorrhiza, ginger, and lime as the feed decreased over time, indicating reduced driving force.Practical applicationsThe application of membrane technology for juices and liquid foods sterilization and concentration has been widely researched. In this research, we evaluated the performance of MF and OMD for the sterilization and concentration of traditional functional drinks. The results indicated that the functional drink can be sterilized by MF with excellent preservation of the active compounds, such as those responsible for the antihyperglycemic and antioxidant activities. The OMD could also concentrate the traditional functional drink, even though concentration polarization posed a challenge to flux stability. The degradation of phenolic compounds and antioxidant activity of the concentrated traditional functional drink was at a minimal value, indicating the promising application of MF and OMD. We believe this article provides new and exciting insights to push forward the sterilization and concentration process in industrial scale.

The Utilization of Secang Wood (Caesalpinia sappan L.) Extract Nanoemulsion as Natural Pigment in Lip Cream Preparations

Lip cream is a semi-solid lipstick preparation. One of the important ingredients in lipcream preparations is a variety of colorants. Secang wood contains the active compound brazilin which gives it a yellow to purple color which can be used as a natural dye. The conventional lip cream preparations from extracts have low homogeneity and unstable, so it is necessary to increase the stability of the preparation by making a nanoemulsion of secang wood extract which is incorporated into the lip cream preparation. The preparation of 10% secang wood extract nanoemulsion preparation using Tween 80, Virgin Coconut Oil (VCO), and PEG 400 with particle size reduction using a bath sonicator. The concentration of the nanoemulsion preparation was 1% (F3) and 3% (F4) incorporated into the lip cream base and conventional lip cream preparations with the same concentration (F1 and F2). The test results for the size of the 10% secang wood extract nanoemulsion globules were 127.54 ± 0.45 nm (PI: 0.3 ± 0.013). The test results for the F3 and F4 nanoemulsion lipcream preparations had a pink to purple color, pH 6-6.4, spreadability of 5.9-6.8 cm, adhesion of 5.5-6 seconds, the preparation was homogeneous and non-irritating, compared with conventional lip cream preparations F1 and F2 are not homogeneous, the color of the lip cream is paler, and the irritating properties are very mild in both formulas. The conclusion of this research is that secang wood extract can be formulated into a nanoemulsion preparation and incorporated into a lip cream preparation.&#x0D; Keywords: Brazilin, Color, Dye, Sonicator Bath

Effects of Aia tempayang on total antioxidant capacity and malondialdehyde level against 7,12-dimethylbenz(a)anthracene-induced breast cancer in rats

Breast cancer occurs due to the abnormal growth and proliferation of cells derived from breast tissue that is caused by continuous stimulation by carcinogenic factors. Breast cancer can be prevented by consuming food that contains antioxidants, such as Aia tempayang. Aia tempayang is a traditional drink originating from West Sumatra. It is made from sappan wood, pangdahai, and basil seeds. Each of its ingredients contains compounds such as antioxidants that can help prevent breast cancer. This study aimed to prove the effectiveness of the administration of Aia tempayang in preventing breast cancer in rats induced by 7,12-dimethylbenz(a)anthracene (DMBA). Healthy female Sprague Dawley rats (35 days old, 150 – 300 g, n = 30) were divided into five groups (n = 6 rats/ group): normal control group (K1); control groups induced by DMBA without treatment (K2); treatment group induced by DMBA + sappan wood 0.072 g + pangdahai 0.012 g + basil seeds 0.045 g (X1); treatment group induced by DMBA + sappan wood 0.144 g + pangdahai 0.024 g + basil seeds 0.09 g (X2); and treatment group induced by DMBA + sappan wood 0.288 g + pangdahai 0.048 g + basil seeds 0.18 g (X3). All rats were gently palpated in the mammary glands to detect the development of any abnormal mass after 5 weeks of intervention. A significant increase in TAC levels was observed in the X1, X2, and X3 groups compared with the K2 group after the treatments (p = 0.000). In addition, there was a significant decrease in the MDA levels in the X1, X2, and X3 groups compared with the K2 group after the treatments (p = 0.000). In conclusion, the administration of Aia tempayang increases the TAC levels and decreases the MDA levels in breast cancer rats induced by DMBA.

Brazilin from Caesalpinia sappan L. as a Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Inhibitor: Pharmacophore-Based Virtual Screening, In Silico Molecular Docking, and In Vitro Studies.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial regulator of low-density lipoprotein cholesterol (LDL-c) levels, as it binds to and degrades the LDL receptor (LDLR) in the lysosome of hepatocytes. Elevated levels of PCSK9 have been linked to an increased LDL-c plasma levels, thereby increasing the risk of cardiovascular disease (CVD), making it an attractive target for therapeutic interventions. As a way to inhibit PCSK9 action, we searched for naturally derived small molecules which can block the binding of PCSK9 to the LDLR. In this study, we carried out in silico studies which consist of virtual screening using an optimized pharmacophore model and molecular docking studies using Pyrx 0.98. Effects of the candidate compounds were evaluated using in vitro PCSK9-LDLR binding assays kit. Eleven natural compounds that bind to PCSK9 were virtually screened form HerbalDB database, including brazilin. Next, molecular docking studies using Pyrx 0.98 showed that brazilin had the highest binding affinity with PCSK9 at -9.0 (Kcal/mol), which was higher than that of the other ten compounds. Subsequent in vitro PCSK9-LDLR binding assays established that brazilin decreased the binding of PCSK9 to the EGF-A fragment of the LDLR in a dose-dependent manner, with an IC50 value of 2.19 μM. We have identified brazilin, which is derived from the Caesalpinia sappan herb, which can act as a small molecule inhibitor of PCSK9. Our findings suggest that screening for small molecules that can block the interaction between PCSK9 and the LDLR in silico and in vitro may be a promising approach for developing novel lipid-lowering therapy.

Pencegahan Stunting Melalui Pelatihan Pembuatan Sirup Kayu Secang dan Jeruk Sambal Kepada Ibu PKK Desa Pal IX, Kalimantan Barat

Desa Pal IX is one of the villages in Kecamatan Sungai Kakap, Kubu Raya Regency which is affected by stunting cases with a stunting case percentage of around 12.7 percent. Sappan wood and limau citrus contain micronutrient compounds in the form of bioactive phenolic compounds and flavonoids, as well as macronutrients in the form of fats and carbohydrates. The aim of implementing this PkM is to provide education and training in making syrup from processed sappan wood and limau citrus as a macro and micro nutrient intake for handling stunting cases in Pal IX village. In general, the stages and planned activities that carried out at this PkM are preparation for activities, implementation of activities, monitoring and evaluation. The method used is the material explanation followed by training on making syrup and how to pack it to produce syrup. The results obtained are processed syrup products from sappan wood and limau citrus. In addition, there was an increase in the average score before and after the workshop, namely an increase in knowledge from a score of 30 to 68.4. Meanwhile, the results of the activity evaluation gave an index value of 86.5%, in the very satisfied category. The workshop activities were able to provide education and skills for PKK organisation in Pal IX Village in recognizing the potential of processed sappan wood and limau citrus syrup in preventing and treating stunting and making syrup product that is rich in macro-micro nutrients.

Cardamom (Elettaria cardamomum) and Sappan Wood (Caesalpinia sappan L.) Proportion Effect on the Antioxidant Activity of "Wedang Kapusecang"

This research explores the impact of varying proportions of cardamom and sappan wood on the antioxidant activity of Wedang Kapusecang, a heated beverage inspired by the traditional Imogiri drink "Wedang Uwuh." Using a factorial Randomized Block Design (RBD) with two factors, the study examines two levels of cardamom (0.5g and 1g) as the first factor and three levels of sappan wood (2g, 5g, and 8g) as the second factor, resulting in six treatment combinations, each duplicated twice. Data analysis employs ANOVA with 1% and 5% confidence intervals, followed by the BNT test (5%) if significant effects are detected. The study includes evaluations of antioxidant activity using the DPPH test and organoleptic aspects such as taste, aroma, and color. The research findings indicate that the most potent antioxidant activity is observed in treatment K2S3 (1g cardamom and 3g sappan wood), with an IC50 parameter of 55,604 ppm, signifying robust antioxidant properties. Organoleptic results identify treatment K2S2 (1g cardamom and 2g sappan wood) as the optimal choice, with moderately liked taste (2.76), moderately liked aroma (2.88), and liked color (3.68). Considering the effectiveness index, treatment K2S2 remains superior, exhibiting an average IC50 antioxidant activity value of 55,604 ppm (strong), with moderately liked taste and aroma ratings, along with a liked color rating.

Toxicity Test on the Combination of Caesalpinia sappan and Zingiber officinale in Rattus norvegicus Induced by Complete Freund's Adjuvant

Highlights:1. This study determined the toxic effects of combining Caesalpinia sappan and Zingiber officinale extracts, aspeople need to be aware of the potential side effects of these common herbal remedies for the treatment ofrheumatoid arthritis.2. The combination of Caesalpinia sappan and Zingiber officinale extracts is efficacious and non-toxic as an antiarthritis treatment, hence, the ingredients can be upgraded to standardized herbal medicines andphytopharmaceuticals. Abstract Sappanwood (Caesalpinia sappan) and red ginger (Zingiber officinale) are plant species that have been studied for theirefficacy in treating inflammation related to rheumatoid arthritis. This study aimed to examine the effects of combiningsappanwood and red ginger in order to determine the potential toxicity of the herbal extracts in medicine. The toxicitytesting was carried out in vivo using 32 Wistar strain male white rats (Rattus norvegicus) grouped into eight groups of four.The rats were injected with complete Freund's adjuvant to induce a chronic inflammatory effect. The eight groups consistedof the negative control group, the positive control group, the normal group, and five treatment groups. This study wasconducted by observing the animals for toxic symptoms and death to determine the safety of the extracts and drugs. Theobservation results were analyzed using a one-way analysis of variance (p&lt;0.05). The analysis results showed that weightgain and relative organ weight among the groups had no significant differences (p&gt;0.05). Microscopic examination of theorgan preparations observed under a light microscope revealed no significant changes or adverse effects in rats treated withthe extracts or drugs. In conclusion, a combination of sappanwood and red ginger ethanol extracts administered orally hasno toxic effect in rats injected with complete Freund's adjuvant.

Biosynthesis of copper oxide nanoparticles using Caesalpinia sappan extract: In vitro evaluation of antifungal and antibiofilm activities against Candida albicans.

Synthesis of nanoparticles using natural organic substances has attracted more attention due to avoiding inorganic toxicity. This work aimed to synthesize copper oxide nanoparticles (CuONPs) using Caesalpinia sappan heartwood extract as a reducing agent. The effects of pH of synthesis reaction were investigated. The obtained CuONPs were characterized using UV-visible spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, and energy dispersive X-ray spectroscopy. Their particle size, size distribution, and zeta potential were determined using photon correlation spectrophotometry. Candida albicans is a major cause of chronic fungal infections due to its biofilms leading to severe drug resistance problems. In this study, in vitro antifungal and antibiofilm activities as well as killing kinetics of the synthesized CuONPs against C. albicans were investigated. Additionally, fungal biofilm was observed by using confocal laser scanning microscopy. The results showed that the pH of the synthesis reaction played an important role in the physicochemical properties and antifungal activities of the obtained CuONPs. CuONPs synthesized at pH 10 and 12 showed the relatively small and narrow size distribution with high negative zeta potential and time-dependent killing kinetics. Confocal laser scanning microscopy confirms obvious fungal biofilm reduction and increased fungal cell death after exposure to CuONPs. These findings suggest the optimal pH of CuONPs synthesis using C. sappan extract as a reducing agent. The results on antifungal and antibiofilm activities indicate that the obtained CuONPs can be a promising agent for treating fungal infection.

Enhancing the color shade and depth of linen fabrics dyed with Caesalpinia sappan L. wood extract using metallic salt mordants

This study demonstrated natural dyeing of linen with a dye extracted from sappan wood (Caesalpinia sappan L.). Four metallic salt mordants, namely (NH4)2Fe(SO4)2, CuSO4, SnCl2, and AlK(SO4)2, referred to as Fe, Cu, Sn, and Al, respectively, were used to improve the dyeing properties. The influences of the pH, dye concentration, mordant type and concentration, and mordanting technique on the dyeing properties were investigated. Dyeing without mordants resulted in a reddish-brown fabric; however, different mordants produced a variety of color shades. In general, the mordant containing Fe yielded the highest color strength, generating a dark gray color very close to black. Crimson and magenta fabrics were obtained using Sn, and pink fabrics were obtained using Al. The samples with the Cu mordant showed a dark and dull purple color. The color strength due to post-mordanting was higher than those due to pre- and meta-mordanting. Dyeing and mordanting increased the ultraviolet protection factor (UPF) of the fabrics. Only the fabrics post-mordanted with Fe exhibited good ultraviolet protection (UPF 15). The concentrations of extractable heavy metals (Fe and Cu) were also determined.

A Comprehensive Review on Bioactive Compounds Found in Caesalpinia sappan.

Sappan wood (Caesalpinia sappan) is a tropical hardwood tree found in Southeast Asia. Sappan wood contains a water-soluble compound, which imparts a red color named brazilin. Sappan wood is utilized to produce dye for fabric and coloring agents for food and beverages, such as wine and meat. As a valuable medicinal plant, the tree is also known for its antioxidant, anti-inflammatory, and anticancer properties. It has been observed that sappan wood contains various bioactive compounds, including brazilin, brazilein, sappan chalcone, and protosappanin A. It has also been discovered that these substances have various health advantages; they lower inflammation, enhance blood circulation, and are anti-oxidative in nature. Sappan wood has been used as a medicine to address a range of illnesses, such as gastrointestinal problems, respiratory infections, and skin conditions. Studies have also suggested that sappan wood may have anticarcinogenic potential as it possesses cytotoxic activity against cancer cells. Based on this, the present review emphasized the different medicinal properties, the role of phytochemicals, their health benefits, and several food and nonfood applications of sappan wood. Overall, sappan wood has demonstrated promising medicinal properties and is an important resource in traditional medicine. The present review has explored the potential role of sappan wood as an essential source of bioactive compounds for drug development.