Butanol Fraction Research Articles

Sucrose esters of aryldihydronaphthalene-type lignans, and antitumor activity of extracts of Moltkiopsis ciliata (Forssk.) aerial parts

One new compound (3f-[(7′R,8′R)-4,4′-dihydroxy-5-methoxy-2,7′-cycloligna-7-en-9-methoxycarbonyl, 9′-carbonyl-O-] -β- D-fructofuranosyl- (2→1)-α- D-glucopyranoside) (Moltkiopsin A) (2) was isolated and identified from the extract of aerial parts of the wild Egyptian plant Moltkiopsis ciliata (Frossk.), family Boraginaceae, for the first time, along with two aryldihydronaphthalene lignans 3f→9′:6f→9-[(7′R,8′R)-4,4′- dihydroxy-3,3′,5-trimethoxy-2,7′-cycloligna-7-en-9,9′-dicarbonyl]-6g-acetyl-α-D-gluco pyranosyl-(1→2) -β-D-fructofuranoside (Trigonotin A) (1) which was reported for the first time from this plant species and a known compound 3f→9′:6f→9-[(7′R,8′R)-4,4′- dihydroxy-3,3′,5-trimethoxy-2,7′-cycloligna-7-en-9,9′-dicarbonyl]-α-D-gluco pyranosyl - (1→2)- β-D- fructofuranoside (Trigonotin C) (3). These compounds were separated and purified using different chromatographic techniques and their structures were elucidated by extensive 1D (1H and 13C NMR), and 2D NMR (COSY, HSQC, and HMBC), besides ESI-MS spectral methods. Extracts were screened as antioxidant, antitumor and antibacterial. The different extracts showed moderate to strong antioxidant capacities in DPPH assays. Ethyl acetate, methylene chloride and crude methanol extracts exhibited the most significant free radicals scavenging activity when compared to the standard antioxidant vitamin C. Hexane and butanol fractions showed the highest cytotoxicity against the cancer cell lines HepG2 and MCF-7.

Investigation of the inhibitory activity of extracts, fractions and secondary metabolites of <i>Silene</i> spp. (Caryophylaceae) and <i>Serratula cupuliformis</i> (Asteraceae) on the «entry» of herpes simplex type 2 into sensitive cells of the Vero line

In vitro analysis of the inhibitory activity of extracts, fractions and secondary metabolites of plants of the genus Silene (Caryophylaceae) and Serratula cupuliformis (Asteraceae) to the «entry» of herpes simplex type 2 (HSV‐2) into sensitive cells of the Vero line.Ethanol extracts and butanol fractions of Silene spp. and Serratula cupuliformis were prepared. The flavonoid shaftoside and the ecdysteroid 20‐hydroxyecdysone from Lychnis chalcedonica were isolated. For analysis of biologically active substances (BAS) the HPLC method was used. The samples were dissolved in distilled water or DMSO. The laboratory strain MS HSV‐2 was grown on Vero cell culture. In vitro analysis of the inhibitory activity of the herbal preparations on «entry» of HSV‐2 was performed in Vero cell culture by direct inactivation (neutralisation) of virions with 103 PFU /ml.Effective concentrations (EC50) have been identified in the range of 50 % from 2.12±0.47 to 180.99±49.24 μg/ml when preparations were dissolved in water and from 1.99±0.44 to 57.42±14.74 μg/ml when they were dissolved in DMSO. Such results for some samples is comparable to the EC50 of comparison preparations (dry ethanol extracts of spice of cloves, chaga and licorice root). The results obtained suggest the presence of BAS in the herbal preparations studied that act destructively on HSV‐2 virions and affect one of the main stages of its «life» cycle – the «entry» of the virus into sensitive cells.

Assessment of Antioxidant Activity, Total Phenolic and Flavonoid Contents of Albizia saponaria L. Bark Extract

Langir (Albizia saponaria L.), belonging to the family of the Fabaceae, is a medicinal endemic plant of South Sulawesi, Indonesia. The Albizia genus shows antioxidant, antibacterial, anthelmintic, antidandruff, and anti-inflammatory properties. In this study, total phenolic (TP) and total flavonoid (TF) extracts from the bark of A. saponaria were screened for robust antioxidant activity in order to identify potential sources of new compounds for use in pharmaceutical formulations in the future. TP and TF of the 96% ethanol extract and fractions (hexane, ethyl acetate, butanol, and water) were calculated by the Folin–Ciocalteu and aluminum chloride procedures, respectively. Furthermore, the antioxidant activity was determined by DPPH free radical scavenging method and ABTS assay. Generally, both extract and fractions showed significant radical scavenging activities. Ethyl acetate fraction exhibited more potent radical scavenging activity in the DPPH method (IC50 35.27 ± 2.85 µg/mL) and ABTS assay (IC50 60.04 ± 0.98 µg/mL), followed by 96% ethanol extract, and hexane, butanol, and water fractions. Furthermore, the highest TP (4.50 ± 0.01 mg/g GAE) and TF (3.55 ± 0.04 mg/g QE) were obtained from ethyl acetate fraction. There was a strong correlation between antioxidant activity with TP (DPPH, R2 = 0.6436; ABTS, R2 = 0.7676) and TF content (DPPH, R2 = 0.5533; ABTS, R2 = 0.5961). The extract’s antioxidant properties may be attributable to its higher phenolic and flavonoid content. In summary, the phenolic and flavonoid content of the ethyl acetate fraction indicates its potential utility as a source of antioxidants.

Antifungal Activity-Guided Analysis of Actinostemma lobatum Extracts through Serial Sub-fractions.

Plants are treasure trove of novel compounds that have potential for antifungal chemicals and drugs. In our previous study, we had screened plant extracts obtained from more than eight hundred plant materials collected in Korea, and found that butanol fraction of the Actinostemma lobatum were most potent in suppressing growth of diverse fungal pathogens of plants. Here in this study, we describe further analysis of the butanol fraction, and summarize the results of subsequent antifungal activity test for the sub-fractions against a selected set of plant pathogenic fungi. This line of analyses allowed us to identify the sub-fractions that could account for a significant proportion of observed antifungal activity of initial butanol fraction from A. lobatum. Further analysis of these sub-fractions and determination of structure would provide the shortlist for novel compounds that can be a lead to new agrochemicals.

Phytochemical Composition and Cytotoxic Activity of Senecio asirensis Hexane Fraction Using In Vitro and In Silico Approaches

Objective Senecio asirensis, an endemic species of Saudi Arabia, has been traditionally used to treat various ailments. The present study aimed to investigate the phytochemical composition, antimicrobial activity, and cytotoxic potential of Senecio asirensis fractions, and to identify the main compounds in the extract that are the most active. Methods The antimicrobial activity was evaluated using disc diffusion assay. Moreover, the cytotoxic activity evaluated using the MTT assay in various cancer cell lines (MCF-7, HepG2, and A549) and validated by molecular docking for the major compound of the most active fraction. Finally, gas chromatography and a mass spectrometer (GC-MS) analysis was carried out to identify the chemical compounds in hexane extract of Senecio asirensis. Results The hexane fraction exhibited significant antimicrobial activity against Gram-positive bacteria ( S. aureus) and yeast organisms ( C. albicans and C. tropicalis), while chloroform and butanol fractions showed no activity against the tested microbial strains. Moreover, in the cytotoxic activity assay, notably, the hexane fraction showed a significantly higher cytotoxic effect on MCF-7. The GC-MS analysis of the hexane fraction revealed the presence of 26 phytoconstituents, with nonacosane being the most abundant compound. Therefore, the molecular docking was employed to validate the cytotoxic activity for the major compound of the most active fraction (nonacosane) against human DNA topoisomerase IIa. Conclusion These findings provide a scientific basis for the traditional use of S. asirensis in medicine and suggest that its bioactive compounds have potential therapeutic applications in anticancer treatments. Further research is needed to isolate and characterize other active compounds and to explore potential synergistic effects among them.

Phenolic Content, Antioxidant Potential, and Antimicrobial Activity of Uvaria chamae (Annonaceae), a Food Plant from Burkina Faso.

The study aimed to evaluate phenolic content and antioxidant and antibacterial potentials of the fractions of the hydroethanolic extract of Uvaria chamae leaves, a food plant from Burkina Faso. Thus, the hexane, dichloromethane, ethyl acetate, and butanol fractions of the hydroalcoholic extract after drying were used to determine phenolic compound content, antioxidant activity, and antimicrobial potential on strains of pathogenic bacteria responsible for food contamination. Phytochemical analyses were performed according to standardized methods, while antioxidant activity was evaluated by DPPH and FRAP methods. The antibacterial activity of the fractions was determined by diffusion and microdilution methods on the agar medium with gentamicin as a reference antibiotic. All the six strains, namely, Salmonella typhi ATCC 19430, Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 9027, Bacillus cereus ATCC 13061, and Listeria monocytogenes ATCC 7644, were sensitive to the fractions tested. Minimum inhibitory concentrations ranged from 37 µg·mL-1 to 1.67 mg·mL-1, respectively, gentamicin and butanolic fractions, while minimum bactericidal concentrations of the fractions ranged from 0.037 to 2.500 mg·mL-1 depending on the bacterial strain. Antioxidant activity varied significantly between fractions. For DPPH free radical scavenging activity, the butanol fraction was the most active, with an IC50 of 280 μg/mL, while the lowest activity (705 μg/mL) was recorded by the hexane fraction. Those of trolox and ascorbic acid used as standards were 80 and 100 μg/mL, respectively. Ferric reducing power (FRAP) ranged from 0.34 to 0.40 mmol EAA/g extract for the hexanic and ethyl acetate fractions, respectively. Phenolic compound contents also varied significantly between fractions. Butanoic and ethyl acetate presented the best contents of total phenolics and flavonoids, respectively. Significant and positive correlations were also recorded between phenolics and antioxidant activities. The antibacterial and antioxidant activities of the active fractions would be related to their richness in bioactive compounds, including phenolic, which are powerful natural antioxidants. U. chamae leaf extracts could therefore be used as dietary supplements to boost the immune system and prevent bacterial infections.

Evaluation of Anti-Obesity and Antidiabetic Activities of Orostachys japonicus in Cell and Animal Models.

Orostachys japonicus is a popular traditional medicinal herb used in Asian countries. This study is focused on evaluating its role in lipid and glucose metabolism in cell and animal models to establish the plant as an anti-obesity and antidiabetic herb. A butanol fraction of O. japonicus was used in the study. The lipid production was evaluated by the Oil Red O technique while the expression of adipogenic markers by Western blotting and RT-PCR using 3T3-L1 preadipocyte. The effect on glucose uptake activity was evaluated in C2C12 myoblast cells. The animal study was carried out in C57BL mice to evaluate anti-obesity activity using the high-fat diet model. The evaluation of serum lipid, blood glucose, adipogenic and fibrosis markers in the liver, and fat deposition in the liver and adipose tissue (by histology) of mice was conducted. Butanol fraction of O. japonicus significantly inhibited the lipid production in the 3T3-L1 cells and reduced the expression of PPARγ, C/EBPα, SREBP-1c and aP2. It enhanced glucose uptake in insulin-resistant C2C12 myoblast cells. It reduced body weight, triglycerides, and blood glucose in the obese mice. It significantly inhibited lipid accumulation in the liver and adipose tissue of obese mice along with suppression of expression of adipogenic and fibrosis markers in the liver. In summary, supporting the previous results, this study helped to establish the potent anti-obesity, antidiabetic, and liver-protecting effect of the butanol fraction of O. japonicus.

Cynara Cardunculus Flavonoids-rich Fraction Alleviates Liver Injury in Mice Overconsumed Fructose Model.

Non-alcoholic Fatty liver disease (NAFLD) is becoming a major global health burden in the world. Cynara cardunculus is an edible plant growing wild in the North of Algeria. Its potential as a source of health-promoting compounds is still underexplored. This study aimed to explore the preventive effect of Cynara cardunculus (C.cardunculus) on the NAFLD model. Total flavonoid contents (TFC) and in vitro antioxidant effects of butanolic (n- BuTOH) and ethyl acetate (EtOAc) fractions on scavenging the ABTS+ radical, inhibition of lipid peroxidation and reducing power proprieties were assessed. The n-ButOH fraction showed the highest TFC and antioxidant capacity in all realized assays. This fraction is used for anti- NAFLD experiments. Adult male Albinos mice were divided into four groups. Group 1 was normal control. Group 2 was watered with 30% of fructose for three weeks to induce the NAFLD model. Group 3 and Group 4 were co-treated with C. cardunculus n-ButOH fractions and Atorvastatin, respectively for three weeks. Blood and livers were collected for biochemical and histological analysis. The C. cardunculus n-ButOH fractions significantly restored levels of transaminases, triglycerides, cholesterol, LDL, glucose and uric acid. The n-ButOH fraction exerted an improving effect on the body and liver weight and liver index. It also significantly corrected the imbalance in liver MDA and GSH levels. The n-ButOH fractions further ameliorated abnormalities in liver histology through suppression of lipid droplet accumulation. This research proves that the flavonoid-rich fraction of C. cardunculus has protective activity against high fructose intake in mice via reversing hyperlipidemia and boosting liver antioxidant capacity.

Allelopathic potential of ethanolic extract and its fractions from leaves of Geonoma schottiana Mart.

Geonoma schottiana Martius (Arecaceae) is an understory palm species widely distributed in Brazil. The objective of this work was to determine the allelopathic potential of Geonoma schottiana leaf extract and its fractions of different polarities and evaluate in vitro and in vivo antioxidant activities. The germination of seeds of Lactuca sativa and Panicum maximum was tested using ethanolic leaf extract and its hexane, dichloromethane, ethyl acetate, and butanol fractions. In vitro antioxidant tests were performed through the DPPH, ABTS, FRAP, and molybdenum tests, whereas the SOD, CAT, and POX enzymatic tests were used to evaluate in vivo antioxidant activity. Chemical analyses were performed through phytochemical screening of the main groups of secondary metabolites by thin-layer chromatography and quantification of the total contents of phenolics, tannins, and flavonoids. The hexane and butanol fractions had allelopathic effects on P. maximum and low activity on L. sativa. The ethyl acetate fraction presented higher antioxidant activity in the DPPH test, whereas butanol presented higher antioxidant activity in the ABTS test. The most active fractions in terms of allelopathic effect interfered with the increases in the production of SOD and POX enzymes. The most polar fractions had higher contents of phenolic compounds. All analyzed chemical groups were found in the phytochemical screening, except the anthracene group.

EVALUATION OF THE ANTI-TYROSINASE-ANTI-AGING POTENTIAL AND METABOLITE PROFILING FROM THE BIOACTIVE FRACTION OF CORN COB (ZEA MAYS L.)

Objective: Maize (Zea mays L.) is a crop that has been widely cultivated in Indonesia. Using corn kernels on a large scale will produce much corn cob waste, usually unused. According to the literature search, corn cobs’ phytochemical studies and pharmacological activities still need to be improved. This study aims to determine the content of secondary metabolites (metabolite profiling) and their antityrosinase and anti-aging potential.
 Methods: Corn cobs were macerated with methanol and fractionated with n-hexane, ethyl acetate, and butanol. The phytochemical profiling approach of the methanol extract was performed by liquid chromatography-mass spectra (LC-MS/MS). Anti-tyrosinase and anti-aging bioactivity were evaluated by thin layer chromatography (TLC)-bioautography and IC50 spectrophotometrically.
 Results: The evaluation results show that the butanol fraction leads to a potential value (IC50 99.92 µg/ml). Several compounds, especially flavonoid compounds (including catechin; kaempferol 3-arabinofuranoside 7-rhamnoside; 6,8-Di-C-beta-D-arabino pyranosyl apigenin; 5,7-Dihydroxy-8,4’-dimethoxyisoflavone) were identified by LC-MS/MS by comparing the molecular mass of MS/MS data with literature data.
 Conclusion: Based on this study, it can be concluded that butanol is the fraction that most actively inhibits tyrosinase, elastase, and collagenase enzymes, which means it potentially becomes a new anti-aging candidate.

Assessment of particulate PAHs toxicity from alcohol-diesel blends fuelled high compression ratio CI engine

Transport and power generation applications will play an important role in current and next-generation compression ignition (CI) engines. Methanol, ethanol, and butanol have emerged as potential alternative fuels for internal combustion engines capable of reducing overall greenhouse gases (GHG) and pollutant emissions. This study deals with the morphological characterization of particulate matter (PM) and toxicity assessment of polycyclic aromatic hydrocarbons (PAHs) for methanol, ethanol and butanol blending with diesel, and provides new findings and results in the specific literature. It defines new limits of alcohol fractions in diesel blends for their applications in CI engines. For the experimental assessment, a single-cylinder CI engine was used with an increased compression ratio (CR) from 17.5 to 26. Alcohol fraction of 20% (v/v) in diesel reduced the PM emissions in the range of 29–38% than diesel. PM was further reduced, up to ∼50%, for higher ethanol and butanol fractions (∼50%), and the soot morphological characteristics exhibit smaller particle sizes (400–600 nm) than diesel (up to 2 μm). Moreover, 20% of alcohol fraction led to a 34–60% reduction in PAHs. The toxicity evaluation of the tested alcohol-diesel blend indicates that high molecular weight PAHs and nitro-PAHs are the main contributors to the overall toxicity. Benzo[a]pyrene and dibenzo-anthracene were the prominent PAHs. Increasing the alcohol blend ratio significantly reduced the toxicity potential, ranging from 20 to 67%.

Evaluation of the Bioactive compounds and its Functional role in the Aquatic weed Pistia stratiotes

Pistia stratiotes (L.) is an invasive aquatic weed known as water lettuce, belonging to the Araceae family. It is commonly used for its therapeutic potency against a number of ailments. This study's purpose is to analyze the pharmacognostical characteristics, screening of phytocompounds, fluorescence evaluation and scavenging activity of the leaf powder of P. stratiotes. The acid insoluble ash (3.22±0.17) was found to be less than total ash (21.85±0.19), water-soluble ash (11.18±0.05) and sulphated ash value (9.63±0.35). The hydro-ethanolic extract was fractionated with solvents of different polarity as per increasing order (hexane, chloroform, ethyl acetate, n-Butanol and aqueous). The moisture content of the leaf powder was 1.70±0.01 gm and the extractive value in aqueous (25.106±1.41) was quantitatively more than that of other solvents. The presence of carbohydrates, phenolic substances, alkaloids, gummy materials, tannins, flavonoids, saponins were revealed by phytochemical studies. The n-Butanol fraction of hydro-ethanolic decoction of leaf powder showed 360±0.01 mg GAE/gdry weight for TPC and 680±0.26 mg RE/g dry weight for TFC. The lowest IC50 value for DPPH was observed in n–Butanol fraction of hydro-ethanolic extract of leaf powder extract having 246.826±17.14 µg/ml, showing the highest free radical scavenging activity.

Inflammatory mediators, oxidative stress and genetic disturbance in rheumatoid arthritis rats supported by alfalfa seeds metabolomic constituents via blocking interleukin-1receptor.

Rheumatoid arthritis (RA) is an autoimmune disease characterized by aggressive cartilage and bone erosion. This work aimed to evaluate the metabolomic profile of Medicago sativa L. (MS) (alfalfa) seeds and explore its therapeutic impact against RA in rats. Arthritis was induced by complete Freund's adjuvant (CFA) and its severity was assessed by the arthritis index. Treatment with MS seeds butanol fraction and interlukin-1 receptor antagonist (IL-1RA) were evaluated through measuring interlukin-1 receptor (IL-1R) type 1 gene expression, interlukin-1 beta (IL-1β), oxidative stress markers, C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2), caspase-3 (Cas-3), intracellular adhesion molecule-1 (ICAM-1), DNA fragmentation, and chromosomal damage. Total phenolics/ flavonoids content in the ethyl acetate, butanol fraction and crude extract of MS seeds were estimated. The major identified compounds were Quercetin, Trans-taxifolin, Gallic acid, 7,4'-Dihydroxyflavone, Cinnamic acid, Kudzusaponin SA4, Isorhamnetin 3-O-beta-D-2'',3'',4''-triacetylglucopyranoside, Apigenin, 5,7,4'-Trihydroxy-3'-methoxyflavone, Desmethylxanthohumol, Pantothenic acid, Soyasapogenol E, Malvidin, Helilandin B, Stigmasterol, and Wairol. Treatment with MS seeds butanol fraction and IL-1RA enhanced all the biochemical parameters and the histopathological features of the ankle joint. In conclusion, Trans-taxifolin was isolated for the first time from the genus Medicago. MS butanol fraction seeds extract and IL-1 RA were considered as anti-rheumatic agents.

In vitro cytotoxicity of Withania somnifera (L.) roots and fruits on oral squamous cell carcinoma cell lines: a study supported by flow cytometry, spectral, and computational investigations.

Oral cancer is a severe health problem that accounts for an alarmingly high number of fatalities worldwide. Withania somnifera (L.) Dunal has been extensively studied against various tumor cell lines from different body organs, rarely from the oral cavity. We thus investigated the cytotoxicity of W. somnifera fruits (W-F) and roots (W-R) hydromethanolic extracts and their chromatographic fractions against oral squamous cell carcinoma (OSCC) cell lines [Ca9-22 (derived from gingiva), HSC-2, HSC-3, and HSC-4 (derived from tongue)] and three normal oral mesenchymal cells [human gingival fibroblast (HGF), human periodontal ligament fibroblast (HPLF), and human pulp cells (HPC)] in comparison to standard drugs. The root polar ethyl acetate (W-R EtOAc) and butanol (W-R BuOH) fractions exhibited the strongest cytotoxicity against the Ca9-22 cell line (CC50 = 51.8 and 40.1μg/mL, respectively), which is relatively the same effect as 5-FU at CC50 = 69.4μM and melphalan at CC50 = 36.3μM on the same cancer cell line. Flow cytometric analysis revealed changes in morphology as well as in the cell cycle profile of the W-R EtOAc and W-R BuOH-treated oral cancer Ca9-22 cells compared to the untreated control. The W-R EtOAc (125μg/mL) exerted morphological changes and induced subG1 accumulation, suggesting apoptotic cell death. A UHPLC MS/MS analysis of the extract enabled the identification of 26 compounds, mainly alkaloids, withanolides, withanosides, and flavonoids. Pharmacophore-based inverse virtual screening proposed that BRD3 and CDK2 are the cancer-relevant targets for the annotated withanolides D (18) and O (12), and the flavonoid kaempferol (11). Molecular modeling studies highlighted the BRD3 and CDK2 as the most probable oncogenic targets of anticancer activity of these molecules. These findings highlight W. somnifera's potential as an affordable source of therapeutic agents for a range of oral malignancies.

In-Vivo Antidiarrhoea Evaluations of Methanol Leaf Extract and Fractions of Chrysophyllum albidum

Background and Objectives: In Nigeria, traditional use of medicinal plants, like Chrysophyllum albidum, for diarrhoea persists. This study assessed its effectiveness in treating diarrhoea in rats. Methods: Extract and Fractions from the plant were tested for antidiarrhoea effect. The process involved methanol extraction and fractionation using hexane, ethyl acetate, and butanol. Rat models were used to evaluate the effects on castor oil-induced diarrhoea, enteropooling, and gastrointestinal motility. Results: Defecation caused by castor oil-induced diarrhoea was inhibited significantly by 53.55, 78.48 and 74.69 % at 500 mg/kg of methanol extract, ethyl acetate, and n-hexane fraction respectively, and by 50.99 and 63.69 % at 250 and 500 mg/kg of butanol fraction respectively. The volume of intestinal content and weight of intestinal content were reduced by 76.74 and 80 % at 500 mg/kg of ethyl acetate fraction. Also, the volume of intestinal content and weight of intestinal content were reduced by 70.93 and 70.83 % at 500 mg/kg of n-hexane fraction. At 250 and 500 mg/kg, the ethyl acetate fraction inhibited intestinal motility by 58.65% and 76.50% respectively. The n-hexane fraction at 500 mg/kg showed a 66.35% inhibition. Conclusion: These fractions from Chrysophyllum albidum leaves exhibit potential antidiarrhoea compounds, as evidenced across all three study models. Ongoing research aims to isolate these compounds for further investigation.

Anti-Inflammatory Flavonoids from Agrimonia pilosa Ledeb: Focusing on Activity-Guided Isolation.

To elucidate the anti-inflammatory properties and constituents of Agrimonia pilosa Ledeb. (A. pilosa), a comprehensive investigation was conducted employing activity-guided isolation. The anti-inflammatory effects were evaluated through an in vitro nitric oxide (NO) assay on lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells. Seven bio-active compounds with anti-inflammatory properties were successfully isolated from the butanol fraction and identified as follows: quercetin-7-O-β-d-rhamnoside (1), apigenin-7-O-β-d-glucopyranoside (2), kaempferol-7-O-β-d-glucopyranoside (3), quercetin (4), kaempferol (5), apigenin (6), and apigenin-7-O-β-d-glucuronide-6″-butylester (7). All isolated compounds showed strong NO inhibitory activity with IC50 values ranging from 1.4 to 31 µM. Compound 6 demonstrated the most potent NO inhibition. Compound 7, a rare flavonoid, was discerned as a novel anti-inflammatory agent, ascertained through its inaugural demonstration of nitric oxide inhibition. Subsequently, a comprehensive structure-activity relationship (SAR) analysis was conducted employing eight flavonoids derived from A. pilosa. The outcomes elucidated that flavones exhibit superior NO inhibitory effects compared to flavonols, and the aglycone form manifests greater potency in NO inhibition than the glycone counterpart. These results highlight A. pilosa as a promising source of effective anti-inflammatory agents and indicate its potential as a health-beneficial dietary supplement and therapeutic material.

Phytochemical analysis, radical scavenging and glioblastoma U87 cells toxicity studies of stem bark of buckthorn (Rhamnus pentapomica R. Parker)

BackgroundDuring the past two decades, the correlation between oxidative stress and a variety of serious illnesses such as atherosclerosis, chronic obstructive pulmonary disease (COPD), Alzheimer disease (AD) and cancer has been established. Medicinal plants and their derived phytochemicals have proven efficacy against free radicals and their associated diseases. The current work was aimed to evaluate the phytochemical constituents of Rhamnus pentapomica R. Parker via Gas Chromatography-Mass Spectrometry (GC–MS) and its antioxidant and anti-glioblastoma potentials.MethodsThe bioactive compounds were analysed in Rhamnus pentapomica R. Parker stem bark extracts by GC–MS analysis, and to evaluate their antioxidant and anti-glioblastoma effects following standard procedures. The stem bark was extracted with 80% methanol for 14 days to get crude methanolic extract (Rp.Cme) followed by polarity directed fractionation using solvents including ethyl acetate, chloroform, butanol to get ethyl acetate fraction (Rp.EtAc), chloroform fraction (Rp.Chf) and butanol fraction (Rp.Bt) respectively. Antioxidant assay was performed using DPPH free radicals and cell viability assay against U87 glioblastoma cancer cell lines was performed via MTT assay.ResultsIn GC-MS analysis, thirty-one compounds were detected in Rp.Cme, 22 in Rp.Chf, 24 in Rp.EtAc and 18 compounds were detected in Rp.Bt. Among the identified compounds in Rp.Cme, 9-Octadecenoic acid (Z)-methyl ester (7.73%), Octasiloxane (5.13%) and Heptasiloxane (5.13%), Hexadecanoic acid, methyl ester (3.76%) and Pentadecanoic acid, 14-methyl-, methyl Ester (3.76%) were highly abundant.. In Rp.Chf, Benzene, 1,3-dimethyl- (3.24%) and in Rp.EtAc Benzene, 1,3-dimethyl-(11.29%) were highly abundant compounds. Antioxidant studies revealed that Rp.Cme and Rp.EtAc exhibit considerable antioxidant potentials with IC50 values of 153.53 μg/ml and 169.62 μg/ml respectively. Both fractions were also highly effective against glioblastoma cells with IC50 of 147.64 μg/ml and 76.41ug/ml respectively.ConclusionPhytochemical analysis revealed the presence of important metabolites which might be active against free radicals and glioblastoma cells. Various samples of the plant exhibited considerable antioxidant and anti-glioblastoma potentials warranting further detailed studies.

Investigating bioactive phytochemicals in bulb and shoot of Allium longisepalum Bertol. from Iran

This study investigated a wide range of bioactive compounds in bulb and shoot of Allium longisepalum Bertol. from Iran. The organs were first extracted with methanol, followed by hexane, dichloromethane, chloroform, ethyl acetate, and butanol, and phytochemicals in fractions were analysed using GC/MS. Butanol exhibited the highest yield percentages in both organs. Palmitic and oleic acid were found in all fractions, while heptadecene-(8)-carboxylic acid, stearic acid, and myristic acid were detected in at least three fractions. Each fraction contained exclusively unique metabolites found only in itself. Hexane, dichloromethane, and chloroform fractions contained specific metabolites with potential antioxidant, allelopathic, antibacterial, and anti-inflammatory effects. Ethyl acetate and butanol fractions revealed significant compounds that have potential in terms of their anticarcinogenic, anti-inflammatory, hair growth-enhancing, and antimicrobial properties, in addition to containing compounds not previously identified in plants. The study analyzes bioactive compound profiles in A. longisepalum, highlighting its chemical diversity and therapeutic potential.

Potential Antioxidant Activity Methods DPPH, ABTS, FRAP, Total Phenol and Total Flavonoid Levels of Macaranga hypoleuca (Reichb. f. & Zoll.) Leaves Extract and Fractions

The Macaranga species (Euphorbiaceae), also known locally as "mahang", has been used in traditional medicine due to its potential health benefits. Around 12 species of the Macaranga have been found to contain more than 50 flavonoid compounds, which are the primary secondary metabolites in this genus. Free radicals can lead to organ damage, resulting in various chronic and degenerative disorders. Active antioxidant compounds can help inhibit the oxidation process caused by free radicals, and phenolic compounds, mainly from the flavonoid group, are thought to be present in Macaranga hypoleuca as a source of natural antioxidants. This study aimed to identify the antioxidant potential of methanol extract and its fractions of M. hypoleuca using the DPPH, ABTS and FRAP methods using a UV-Vis spectrometer method, as well as to determine total phenol and total flavonoid levels. The highest antioxidant activity of the DPPH and ABTS methods was found in the ethyl acetate fraction with IC50 values of 14.31 and 2.10 mg/L respectively. Meanwhile, the highest antioxidant activity of the FRAP method was found in the butanol fraction with an IC50 value of 0.48 mg/L. The highest total phenol and flavonoid content was found in the butanol fraction at 27.51 mg GAE/g and 88.42 mg QE/g respectively.

Antitumor activity of membranes associated with Acmella oleracea extract.

Epithelial cancers, such as epidermoid cancer and some adenocarcinomas, affect surface areas that are generally more accessible to various treatments. However, this group of tumor cells has an aggressive behavior, leading to a high annual mortality rate. The development of a biomaterial that is non-invasive, can kill tumor cells, and prevent opportunistic infections is the basis for the treatment for this type of cancer. Therefore, the objective of this study was to develop a biomaterial from chitosan and A. oleracea extracts that exhibits cytotoxic action against the HEp-2 tumor cell line. Dried crude 90% ethanol extracts were obtained through ultrasound-assisted maceration, followed by liquid-liquid extraction to yield the butanol fraction. From these extracts, chitosan membranes were developed and evaluated for their antitumor activity against HEp-2 using viability tests with crystal violet and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, in addition to a wound healing test. The cytotoxic assays indicated a significant reduction in cell density and mitochondrial activity, especially at the concentration of 1000 µg/mL of crude extract. The butanol fraction had minimal effects on mitochondrial activity. The wound healing test demonstrated that the biomaterial and extract prevented closure of the wound created in the cell monolayer within 48 h of incubation and caused changes in cell morphology. In view of this, we concluded that a chitosan membrane associated with a 90% ethanol extract of Acmella oleracea exhibited cytotoxic activity is a potential alternative treatment for superficial cancers.