Healthcare practitioners in ART setting work under high pressure environment with heavy caseloads and patients facing a lot uncertainty and uncontrollability. This study aims at evaluating a professional development program which was develop to cultivate compassion satisfaction (CS), which refers to the pleasure derived from doing health care work effectively, and enhancing their ability to receive gratification from caregiving. This is a quasi-experimental study. Twenty-five healthcare practitioners from 11 licensed ART clinics in Hong Kong, including obstetricians and gynecologists, nurses, social workers and embryologists, attended a professional training course on infertility counseling. Effectiveness of the program was evaluated before and after the implementation of the program. The professional development program was composed of nine three-hour weekly sessions, including components such as self-reflective activities, mindfulness, compassionate meditation and personal reflection on vulnerabilities and resilience, life and death, as well as pain and suffering. All of them were invited to complete a self-administered questionnaire before (T0) and after (T1) the course, which contained Professional Quality of Life (ProQol) measuring CS, compassion fatigue (CF), and other validated measures related to psychological wellbeing. Participants showed significant improvement in CS after attending the course (Pre-course: 33.94 + 4.4; Post-course: 36.35 + 3.82, t=-3.29, p<0.001). There was no significant change in the subscales of Burnout and Secondary Trauma. Moreover, healthcare practitioners who were younger in age, worked longer in medical field (t=-5.196, p<0.035) but shorter in ART setting (t=-3.042,p<0.014) showed greater difference. In addition, those who were with lower educational level (t=-3.45,p<0.014) and had religious belief (t=-7.071,p<0.002) also showed significant improvement in CS. The professional quality of life among healthcare practitioners comprises of both CS and CF. Although no significant change was found in CF, the program could still enhance CS among healthcare practitioners in ART setting. Regarding CF being the emphasis of many researches, enhancing CS involved in caring thus provides a positive aspect in cultivating job satisfaction and positive feelings in healthcare profession. Apart from preventing burnout, it can shed light on how to enhance the quality of human services in the field of ART. Therefore, CS should be taken into consideration in professional training on infertility counseling.