Cities with different background climates experience different thermal environments. Many studies have investigated land cover effects on surface urban heat in individual cities. However, a quantitative understanding of how background climates modify the thermal impact of urban land covers remains elusive. Here, we characterise land cover and their impacts on land surface temperature (LST) for 54 highly populated cities using Landsat-8 imagery. Results show that urban surface characteristics and their thermal response are distinctly different across various climate regimes, with the largest difference for cities in arid climates. Cold cities show the largest seasonal variability, with the least seasonality in tropical and arid cities. In tropical, temperate, and cold climates, normalised difference built-up index (NDBI) is the strongest contributor to LST variability during warm months followed by normalised difference vegetation index (NDVI), while normalised difference bareness index (NDBaI) is the most important factor in arid climates. These findings provide a climate-sensitive basis for future land cover planning oriented at mitigating local surface warming.