Buccal Tubes Research Articles

Molar Buccal Tubes Front and Back Openings Dimensions and Torsional Play

Background/: Buccal tubes are orthodontic attachments used on the posterior teeth instead of bands, so it is important to focus on the effect of their properties on orthodontic treatment. The aims of the present in vitro study are to evaluate and compare the buccal tube front and back openings dimensions and the torsional play angle of six different brands. Materials and Methods: The samples consisted of Single bondable, non-convertible first molar buccal tubes from six brands supplied from six companies (Dentaurum, Forestadent, Ormco, 3M, American Orthodontic, A-Star). Regarding tube opening dimension, ten buccal tubes of each brand were examined by an optical microscope. Each tube was fixed during examination using synthetic mud and oriented for observation of the front and back slot openings. A picture was taken for both tube openings and the result appeared on the computer's screen where width and height measurements were made. While regarding torsional play angle, ten buccal tubes of each brand were used. Each tube was fixed on a metal block attached to a surveyor base. Then an L-shaped wire was inserted inside the front opening of the tube. Two photographs were taken, one with the wire in free fall position and the other with the wire elevated by a 10g weight with the same angle of shooting as the first photograph. Later, the two images were superimposed in Adobe Photoshop program, and an electronic MB-ruler Software was used to calculate the angle which represents the torsional play within each tube. The data were then statistically analyzed using ANOVA and LSD tests. Results: There are marked differences between measured tube dimensions and the manufacturer stated dimensions with the front tube openings being generally larger than the back opening dimensions. Furthermore, the torsional play angle was highest in A-Star and smallest in Ormco's tubes. This angle was significantly correlated to the height of the tube front opening. Conclusion: It can be concluded that tube dimension varies among different companies and effect greatly torsional play angle.

Molar Buccal Tubes Front and Back Openings Dimensions and Torsional Play

Molar Buccal Tubes Front and Back Openings Dimensions and Torsional Play

Pollen- and Non Pollen Palynomorphs- Analyses from Svalbard

We analysed fifteen samples of a shallow profile “Sverrefjellet Volcano “, located in the NW part of Svalbard – Bockfjord region (see Fig.1). Frequency of pollen grains, spores and NPP (Non Pollen Palynomorphs) was quite low. Only the local taxa (e.g. Salix and Cyperaceae) produced pollen in greater abundance. The pollen spectrum was considerably varied, although pollen grains and spores of most taxa were present in low abundances. This was due to low pollen and spores productivity of the Arctic flora, which propagates mostly in a vegetative way. To obtain as much information from the profile under study as possible, we analysed the non- pollen palynomorphs as well. We succeeded above all in determination of eggs, buccal tubes and claws of the Tardigrada phylum (Jankovská 1991). The determination has been recently verified by specialists in the field, prof. L. Kaczmarek and dr. M. Roszkowska (in Jankovská et al. 2016). The results of the pollen- and NPP analysis are presented in pollen- and NPP diagram, which represents a picture of the species-poor Arctic flora.

Impact of different rectangular wires on torsional expression of different sizes of buccal tube.

BackgroundTorsions in rectangular wires are the essential part of corrections in the finishing stage of treatment. Moreover the greatest amounts of torques are applied in the molar areas. a clinically effective moment is between 5 and 20 Nmm. In this study we have decided to evaluate the impact of different tube sizes and different dimensions of wires with different modulus of elasticities on the amount torsional bond strength of molar tubes.Material and Methods60 human impacted molar teeth were collected. A buccal tube was bonded on the buccal surface of all the samples by using light cured adhesive resin. After that, the teeth were mounted in a hard acrylic block. According to the size of buccal tube and the rectangular wires to be tested 4 groups will be designed. Torsional force was applied by instron machine. The torque angle at 5Nmm and at 20Nmm point will be calculated: which means, how many degrees of torque is required to reach the maximum 20Nmm moment from the minimum 5Nmm.One-way ANOVA was used to compare torque angle in all of the groups.ResultsThe least amount of clinically significant angle was 2.2 ᵒ in the 0.017×0.025 SS and the largest amount of it was 23.7 ᵒ in the 0.017×0.025 TMA in 0.018×0.025 slot molar tube. But, this angle was 19.9 ᵒand 13.6 ᵒ in 0.019×0.025 SS and 0.019×0.025 TMA archwire in 0.022×0.028 molar tube.ConclusionsThe 0.017×0.025 SS archwire in 0.018×0.025 molar tube had the lowest clinically significant angle. The largest amount was seen in group 0.017×0.025 TMA in 0.018×0.025 slot molar tube. Key words: Torsional efficacy, rectangular wires, buccal tubes, torque angle.

Is the gut content ofMilnesium(Eutardigrada) related to buccal tube size?

Milnesium, with its relatively short and wide buccal tube, is considered carnivorous. Species in this genus exhibit differences in buccal tube length, standard buccal tube width, and the buccal tube length/width ratio. To determine whether buccal tube size is correlated with the type of prey, ˜4000 specimens of various Milnesium species were examined. Among those with identifiable gut contents, ˜97% contained tardigrades, rotifers, or both, whereas only ˜3% contained nematodes or amoebas. In total, 189 females with guts containing only tardigrades, only rotifers, or both were analysed with general linear model multivariate analysis. Milnesium specimens containing only tardigrade remnants were larger and had longer and wider buccal tubes than those containing only rotifers. Those with only tardigrades in the gut also had a significantly lower buccal tube length to width ratio than those with only rotifers in the gut. Specimens with a mixed diet (rotifers and tardigrades) had intermediate values of buccal tube width and length, and these differed significantly from individuals containing rotifers or tardigrades only. Variation in buccal tube dimensions was linked with interspecific and age (size)-related factors, and differences in buccal tube dimensions associated with gut content remained significant, even when the analysis was controlled for the interspecific variation. No evidence of cannibalism was observed.

An experimental test of eutardigrade preparation methods for light microscopy

Given the constantly growing number of described tardigrade species and the scarcity of taxonomically meaningful morphological characters that these microinvertebrates exhibit, in many cases morphometric traits are the primary or even the only means of classic species differentiation, especially between closely related taxa. However, the validity of comparisons is contingent on the assumption that the mounting does not significantly affect the dimensions of morphometric traits, meaning that specimens mounted on different microscope slides may be compared with confidence. Despite the importance of the accuracy of morphometric measurements in tardigrade taxonomy and a long-standing supposition that the pressure exerted by the coverslip on a tardigrade specimen mounted on a microscope slide may deform some of the taxonomically important morphometric traits, the effect of mounting on specimen quality has not yet been addressed conclusively. In this paper we experimentally tested nine mounting methods using a lab-bred clonal strain of Milnesium cf. alpigenum Ehrenberg, 1853. Among all known tardigrades, species of the genus Milnesium Doyère, 1840 exhibit the widest buccal tubes, thus they provide the most sensitive gauge of deformation caused by coverslip pressure. Moreover, using equal-age clonal animals reared under uniform lab conditions allowed us to minimize background noise in the data. Our study showed that among the nine mounting methods tested, thermal inactivation of animals at 60 °C for 30 min followed by briefly pressing the coverslip with an entomological pin produced specimens fixed optimally for morphometry and imaging (i.e. maximally stretched and levelled but not deformed).

Clinical Evaluation Of The Periodontal Condition During Fixed Orthodontic Treatment

Throughout orthodontic treatment, oral hygiene is particularly important and must be rigorously controlled throughout therapeutic act. The aim of our clinical study was to examine the periodontal status of patients before, during and after orthodontic appliances are used. 50 patients were selected, plaque index, gingival index, modified papillae bleeding index and clinical probing depth was evaluated on anchorage teeth, separately those with orthodontic bands and tubes. One of the most common findings during this kind of treatment is gingival alteration in the posterior region. Our practical observations showed a different status of these alterations at patients wearing bands on the anchorage teeth versus those with buccal tubes. Using some of the indices which describes the periodontal status at adult and adolescent patients, we found out that adolescents present a higher plaque index, and the accumulation of dental plaque is more severe when bands are used in both categories. Another finding, which is very important in everyday practice is that gingival inflammation and plaque accumulation decrease one month after debonding.

Remains of non-pollen-palynomorphs – tardigrades from Spitsbergen found during pollen analyses

ABSTRACTPollen- and non-pollen-palynomorphs (NPP) analytical studies of the northwestern part of Spitsbergen were conducted between 1988 and 1991. As well as pollen from local native flora and more dispersed species, some well preserved remains of tardigrada exuvia, buccal tubes and eggs were found. This study reviewed the remains of at least six tardigrade taxa reported:Dactylobiotus ambiguous, Paramacrobiotus richtersigroup,Richtersius coronifer, Macrobiotus hufelandigroup,Macrobiotus peterseniandMinibiotuscf.intermedius, which are reassessed and determined more accurately. These findings provide some new insights into the past environmental conditions and changes for Spitsbergen. Based on the present research it can be concluded that tardigrade remains are frequent NPP elements of pollen analyses from lake, peat bogs and detritus sub-fossil sediment cores, at least in polar regions. It can also be stated that tardigrades can be considered indicators in further palaeontological studies helping to reconstruct past environmental conditions (for example humidity) for some regions. However, the knowledge of tardigrades in these types of analyses is still rather poor.

Orthodontic correction of severely rotated maxillary central incisor in a diabetic adult

Background: Orthodontics has recently seen an increase in the number of adult population seeking treatment. Financial dependency, increasing awareness and availability of service can be the reasons behind this rise. Though, clinical myths regarding duration, effectiveness of treatment, associated systemic conditions still exist, these should be of no concern and with adequate monitoring and procedural modifications, conventional orthodontic treatment is possible.Case description: A 58 year old Type II diabetic male presented to orthodontic clinic with unesthetic gap between upper front teeth. The history revealed extraction of painful mesiodens. On examination, the patient had Class I molar, canine and incisor relationship. 21 was rotated with 5mm of space between central incisors. Fixed orthodontic treatment was planned after physician consultation regarding his diabetic condition. Bondable buccal tubes instead of bands were used in first molars, 0.022” Roth brackets were bonded on other maxillary teeth. The wire gradually progressed from 0.014”NiTi, 0.016”NiTi to 0.018”SS. Lingual button was attached on the labial and lingual surface of 21 to apply couple. After the correction of rotation of 21, remaining space closure with esthetic contouring of 21 was done. Maintenance of adequate oral hygiene was reinforced throughout the treatment period. Fixed lingual retainer was bonded and pericision performed to retain the achieved result.Conclusion: Orthodontic treatment can be carried out in diabetic adults with good glycemic control to achieve esthetic results; however, measures for maintenance of adequate oral hygiene should be undertaken. Interdisciplinary approach involving restorative procedures can enhance the esthetics achieved.JCMS Nepal. 2015;11(3):30-34

B14-O-05Correlative imaging analysis of Tardigrada (water bears) under the active and dehydrated states by X-ray micro-computed tomography, electron microscopy and confocal microscopy

Some Tardigrades can survive in the dehydrated state called ‘tun’. However how they can achieve such a remarkable ability of anhydrobiosis has not been well understood. We studied the internal structure of the tuns of three Tardigrade species by mCT at SPring-8 (BL20XU and BL47XU) at the resolution of 83〜500nm: Milnesium tardigradum, Ramazzottius varieornatus, as well as Macrobiotus sp. collected on the Harima Science campus of the Univ. of Hyogo. Previously we identified stylets, buccal tubes, pharynx, and eggs (2010A1350, 2013A1319). Here we succeeded in visualizing organelles with less X-ray absorption (2014B1568). By comparing the mCT images with the ones by confocal microscopy, we identified them as droplets in the coelomic cells in the tun. This allowed us to map the 3D distribution of droplets in a coelomic cell as well as coelomic cells in a tun (Fig). We also visualized the structure of organs in the active state, including the brain, eyes, ganglia, muscles, and digestive organs by mCT and TEM. We are now analyzing the structural change during the recovery (rehydration) by cryo-mCT, and the nano-structure of the tun by electron microscopy.

Glass bead sterilizer comprehensively defeats hot air oven in orthodontic clinic

Background: It is necessary to 'try in' several bands before the correct one is selected. A possible concern with re-using such bands is the lack of cross-infection control. Aim and Objectives: To determine whether such bands could be successfully decontaminated with Glass bead sterilization so that they could be re-used without a cross-infection risk. Materials: Custom made molar bands were taken and buccal tubes,lingual sheath and lingual cleat were welded under strict aseptic conditions. Methods: Samples were divided into 2 groups i.e. A and B, based on mode for sterilization. Sterilized attachments were placed in each of 2 conical flask. The bacteria spores were inoculated into both flask under strict aseptic conditions. Bacteria Bacillus subtillis and Staphylococcus albus species were allowed to multiply in individual flasks filled with BHI broth for 24 hours. Bands from 1st group were placed in a glass bead sterilizer. For the 2 nd group i.e. hot air oven group, all bands were placed together. After sterilization bands were removed and placed in freshly sterilized 500ml conical flask containing BHI broth for 24 hours in the incubator. The following day randomly 4 attachments were selected from each group and streaked on blood agar culture plates. Results: After sterilization and on further incubation in BHI broth for 24 and 48 hrs. Respectively no growth was seen. Conclusion: 1 hr. of Hot Air Oven sterilization (excluding pre sterilization heat up time and post sterilization cooling time) at 190°C is as effective as 3 min of Chair side Glass Bead sterilization.

Release of nickel and chromium ions in the saliva of patients with fixed orthodontic appliance: An in-vivo study.

Introduction:Various components of fixed orthodontic appliances are continuously interacting with saliva and other fluids in the mouth releasing various metal ions including nickel and chromium that can cause damaging effects if their concentration exceeds above the toxic dose.Aim:To determine and compare the level of nickel and chromium in the saliva of patients undergoing fixed orthodontic treatment at different time periods.Materials and Methods:The sample of saliva of 13 patients was taken at different time periods that is: Group 1 (before appliance placement), Group II, III, and IV (after 1-week, 1-month, and 3 months of appliance placement respectively). The fixed appliance comprised of brackets, bands, buccal tubes, lingual sheath, transpalatal arch and wires composed of Ni-Ti and stainless steel. The level of ions was determined using graphite furnace atomic absorption spectro-photometry. The data thus obtained were statistically analyzed using SPSS Statistical Analysis Software (Version 15.0).Results:Level of nickel and chromium in saliva was highest in Group II and lowest in Groups I for both the ions. On comparison among different Groups, it was statistically significant for all the groups (<0.001) except between Group III and Group IV.Conclusion:The release of nickel and chromium was maximum at 1-week and then the level gradually declined. These values were well below the toxic dose of these ions. The results should be viewed with caution in subjects with Ni hypersensitivity.

In vivo plaque count of Streptococcus Mutans around orthodontic brackets bonded with two different adhesives

The prevention of the enamel demineralization at the periphery of the brackets is a significant challenge to orthodontic professionals. The aim of this clinical study was to compare the Streptococcus mutants count in the plaque surrounding two orthodontic adhesive types, Fuji Ortho LC and Enlight (Ormco). Materials and methods: A total of 13 patients (7 males and 6 females) needing fixed orthodontic appliance therapy were participated. A split mouth technique was followed with appliances bonded by two orthodontic adhesive types, Fuji Ortho LC and Enlight (Ormco). Saliva was collected before placement of appliances (T0) and again at three weeks (T1) and six weeks (T2) after placement of appliances. Plaque was collected from areas adjacent to brackets and buccal tubes at three weeks (T1) and six weeks (T2) after placement of appliances. The numbers (colony-forming units) of Streptococcus mutans were determined with the side-specific modified Strip-Mutans. Results: No significant modification in the number of Streptococcus mutans CFU in saliva was observed at both time intervals (T1) and (T2) after placement of appliances. The number of Streptococcus mutans CFU in plaque at both time intervals (T1) and (T2) was statistically lower in sites adjacent to Fuji Ortho LC than in those adjacent to Enlight (Ormco) adhesive. Conclusion: plaque surround brackets and tubes bonded with Fuji Ortho LC adhesive harbor less Streptococcus mutans and this will aid in prevention of enamel demineralization.

Clinical comparison of two kinds of orthodontic adhesives bonding buccal tubes of posterior teeth

Clinical comparison of two kinds of orthodontic adhesives bonding buccal tubes of posterior teeth

Shear bond strength of orthodontic buccal tubes to porcelain.

Background:Bonding of molar tubes is becoming more popular in orthodontics. Occasionally, these bonding are done on posterior porcelain crowns or bridges. The purpose of this study was to evaluate the shear bond strength of buccal tubes on feldspathic porcelain crowns with two different methods.Materials and Methods:Forty porcelain right molar crowns were fabricated for this study. The crowns were randomly divided into two groups. In group 1, the crowns were etched with 9.6% hydrofluoric acid, silane coupling agent applied, coated with bonding primer and bonded with Transbond XT (3M Unitek, Monrovia, Calif). In group 2, the crowns were etched with phosphoric acid 37%, silane coupling agent applied, coated with bonding primer and bonded with Transbond XT. All the crowns were stored for 24 hours at 37°C and thermo-cycled before the shear bond test. The analysis of variance (ANOVA) was used to determine whether significant difference were present between the groups.Results:The results of the analysis of variance (F = 0.23) indicated the shear bond strength of group 1 (3.57 ± 0.87 MPa) was not significantly different (P > 0.05) from group 2 (3.46 ± 0.65 Mpa). Fisher's exact test for the adhesive remnant index (ARI) revealed significant difference between both groups (P < 0.05). Eighty percent of group 1 buccal tubes failed at buccal tube/resin interface and eighty percent of group 2 mostly failed at porcelain/resin interface.Conclusion:Etching with phosphoric acid with the use of silane coupling agent would be safer and should make it easier for clinicians to clean the adhesive on the porcelain surface after debonding.

A new water bear, Minibiotus julianae, from the Caribbean Island of Dominica (Tardigrada: Eutardigrada: Parachela: Macrobiotidae)

A new species of tardigrade, Minibiotus acadianus, is described from a moss sample collected in June 2009 from a rainforest palm tree on the island of Dominica, West Indies. The new species has a buccal tube with a single anterior curvature, two macroplacoids, and a microplacoid. The cuticle is smooth with small, evenly-distributed circular or elliptical pores. Eggs have a reticulated shell surface and short inverted goblet egg process; a dentate margin on the distal dish of the processes bears 10–12 short teeth. The new species is most similar to Minibiotus acadianus Meyer & Domingue, 2011; both have wider buccal tubes than other species in the genus. The new species is easily distinguished from M. acadianus in lacking cuticular gibbosities and in some characters of the egg.

The effect of retentive groove, sandblasting and cement type on the retentive strength of stainless steel crowns in primary second molars - An in vitro comparative study

This in vitro study was conducted to find out the effect of retentive groove, sand blasting and cement type on the retentive strength of stainless steel crowns in primary second molars. Thirty-two extracted intact human maxillary and mandibular primary second molars were embedded in aluminum blocks utilizing autopolymerising acrylic resin. After tooth preparation, the 3M stainless steel crown was adjusted to the prepared tooth. Then weldable buccal tubes were welded on the buccal and lingual surfaces of each crown as an attachment for the testing machine. A full factorial design matrix for four factors (retentive groove placement on the tooth, cement type, sandblasting and primary second molar) at two levels each was developed and the study was conducted as dictated by the matrix. The lower and upper limits for each factor were without and with retentive groove placement on the tooth, GIC and RMGIC, without and with sandblasting of crown, maxillary and mandibular second primary molar. For those teeth for which the design matrix dictated groove placement, the retentive groove was placed on the middle third of the buccal surface of the tooth horizontally and for those crowns for which sandblasting of the crowns are to be done, sandblasting was done with aluminium oxide with a particle size of 250 mm. The crowns were luted with either GIC or RMGIC, as dictated by the design matrix. Then the retentive strength of each sample was evaluated by means of an universal testing machine. The obtained data was analyzed using ANOVA for statistical analysis of the data and 't'- tests for pairwise comparison. The mean retentive strength in kg/cm 2 stainless steel crowns luted with RMGIC was 19.361 and the mean retentive strength of stainless steel crowns luted with GIC was 15.964 kg/cm 2 with a mean difference of 3.397 kg/cm 2 and was statistically significant. The mean retentive strength in kg/cm 2 of stainless steel crowns, which was not sandblasted, was 18.880 and which was sandblasted was 16.445 kg/cm 2 with a mean difference of 2.436 kg/cm 2 . These results were again statistically significant. It was found that the crowns luted with resin-modified glass ionomer cements (RMGIC's) offered better retentive strength of crowns than glass ionomer cements (GIC) and stainless steel crowns which were cemented without sandblasting showed higher mean retentive strength than with sandblasting of crowns. The presence of groove did not influence the retentive strength of stainless steel crowns.

Metallic ions released from stainless steel, nickel-free, and titanium orthodontic alloys: Toxicity and DNA damage

The aims of this study were to determine the amounts of metallic ions that stainless steel, nickel-free, and titanium alloys release to a culture medium, and to evaluate the cellular viability and DNA damage of cultivated human fibroblasts with those mediums. The metals were extracted from 10 samples (each consisting of 4 buccal tubes and 20 brackets) of the 3 orthodontic alloys that were submerged for 30 days in minimum essential medium. Next, the determination of metals was performed by using inductively coupled plasma mass spectrometry, cellular viability was assessed by using the tetrazolium reduction assay (MTT assay) (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide), and DNA damage was determined with the Comet assay. The metals measured in all the samples were Ti(47), Cr(52), Mn(55), Co(59), Ni(60), Mo(92), Fe(56), Cu(63), Zn(66), As(75), Se(78), Cd(111), and Pb(208). The cellular viability of the cultured fibroblasts incubated for 7 days with minimum essential medium, with the stainless steel alloy submerged, was close to 0%. Moreover, high concentrations of titanium, chromium, manganese, cobalt, nickel, molybdenum, iron, copper, and zinc were detected. The nickel-free alloy released lower amounts of ions to the medium. The greatest damage in the cellular DNA, measured as the olive moment, was also produced by the stainless steel alloy followed by the nickel-free alloy. Conversely, the titanium alloy had an increased cellular viability and did not damage the cellular DNA, as compared with the control values. The titanium brackets and tubes are the most biocompatible of the 3 alloys studied.

Estudo in vitro da resistência ao cisalhamento da colagem direta de tubos ortodônticos em molares

INTRODUÇÃO: apesar da colagem direta despender menor tempo clínico, com maior preservação da integridade gengival, ainda hoje se observa uma alta incidência de bandagem dos molares. Portanto, torna-se interessante a idealização de recursos para o aumento da eficiência desse procedimento para dentes submetidos a maiores impactos mastigatórios, como, por exemplo, os molares. OBJETIVO: esse estudo teve o propósito de avaliar se a resistência à adesão com a aplicação de uma camada de resina adicional na região oclusal da interface tubo/dente aumenta a qualidade do procedimento de colagem direta de tubos em molares. MÉTODOS: selecionou-se uma amostra composta por 40 terceiros molares inferiores, que foram aleatoriamente divididos em 2 grupos: Grupo 1 - colagem direta convencional, seguida pela aplicação de uma camada de resina na oclusal da interface tubo/dente; e Grupo 2 - colagem direta convencional. O teste de resistência ao cisalhamento foi realizado 24 horas após a colagem, utilizando-se uma máquina de ensaio universal, operando a uma velocidade de 0,5mm/min. Os resultados foram analisados por meio do teste t independente. RESULTADOS: os valores médios obtidos nos testes de cisalhamento foram: 17,08MPa para o Grupo 1 e 12,60MPa para o Grupo 2. O Grupo 1 apresentou uma resistência ao cisalhamento estatisticamente significativa mais alta do que o Grupo 2. CONCLUSÃO: a aplicação de uma camada adicional de resina na oclusal da interface tubo/dente aumenta a qualidade da adesão do procedimento de colagem direta de tubos ortodônticos em molares.

A Comparative Study of Impression Procedures for Distal Extension Removable Partial Dentures

This study was carried out with the purpose of comparing three impression methods as to which of them placed tissues most favorably. The methods used were Hindels method, selective tissue placement method and functional reline method. The measurements obtained were analyzed to determine which of the three impression methods placed the mucosal tissues maximally. To compare and measure tissue placements, autopolymerizing acrylic resin platforms were constructed to the height of the occlusal surfaces of the remaining teeth. 15 orthodontic buccal tubes were placed on each side of the platform. They were arranged in three sets of five and attached to the platform over selected reference regions by means of autopolymerizing resin. The selected reference areas were in anterior, middle and posterior areas of the ridge on either side. No significant difference was seen in tissue placement in the anterior middle and posterior regions in each of the three methods when each method was assessed separately. Selective tissue placement method placed the tissues maximally (7.547 mm) followed by Hindels method (7.2110 mm) and the least placement was by functional reline method (5.856 mm). Tissue placement was significantly higher in Hindels method as compared to functional reline method (p < 0.001). Tissue placement was maximum in the posterior region, followed by the middle region and least in the anterior region of the mandibular ridge for all three methods. Selective tissue placement method showed the maximum overall tissue placement followed by the Hindels method and minimum placement was by functional reline method. Selective tissue placement method provided maximum overall tissue placement and can be a preferred technique for impression making for bilateral distal extension removable partial denture fabrication.