BT-TI Group Research Articles

Elasticity Profile of Skin, Neuronal, Cardiac, and Skeletal Muscle Cells after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation

The present study aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on elasticity of skin, heart, muscle, and neuronal cells in the H9C2 (rat cardiomyocytes), C2C12 (mouse myoblast cells), HaCaT (human keratinocytes), and SH-SY5Y (human neuroblastoma cells) cell line in DMEM medium. The test formulation constituents were divided into two parts; one section was defined as untreated test formulation (UT), while the other portion of test formulation received Biofield Energy Healing/Blessing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing/Blessing Treatment and divided as Biofield Energy Treated/Blessed (BT) and untreated (UT) test items. MTT data showed that the test formulation in various concentrations was found as safe and nontoxic in the tested concentrations with viability range from 73% to 307%. Young’s modulus (YM) is a measure of cell stiffness, a decrease in YM value indicates increase elasticity of the cells and vice-versa. YM in H9C2 cells were decreased by 9.6% and 66.1% in the BT-DMEM + UT-TI group at 0.1 and 1 µg/mL respectively, as compared with untreated test group. However, C2C21 cells showed increased YM by 443.9% at 1 µg/mL in the UT-DMEM + BT-TI group, while 869.6% increased YM in the BT-DMEM + UT-TI group at 1 µg/mL as compared with untreated test group. However, 314% increased YM was reported in the BT-DMEM + BT-TI group at 1 µg/mL as compared with the untreated test group. However, the value of YM was significantly decreased in the HaCaT cell line by 247.7% (at 1 µg/mL), 225.8% (at 0.1 µg/mL), and 97.9% (at 1 µg/mL) in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. In addition, YM was significantly decreased in the SH-SY5Y cell line by 92.6%, 18.1%, and 26.6% at 1 µg/mL in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. Overall, the results showed the significant decreased YM among the SH-SY5Y, HaCaT, and H9C2 cells, while it was increased in the C2C21 cell line. Thus, the mechanical properties of cells such as cellular function, including shape, motility, differentiation, division, and adhesion to its surrounding extracellular matrix were improved. Overall, it can be useful in many disease progressions with improved cellular elasticity and its associated complications/symptoms.

Biofield Energy Therapy: Role in Multiple Organ Health Specific Biomarkers in Cell-Based Assay

The major cause of high rate of mortality is the multiple organ dysfunction among critical care healthcare services. The aim of the current study was to evaluate the impact of the Biofield Energy Treated test formulation using standard and specific cell lines related with vital organs functioning. The test formulation and the specific cell media was divided into two parts; one part was untreated (UT) and other part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Sakina Aleemah Ansari, USA and labeled as the Biofield Energy Treated (BT) test formulation/media. The test formulation was tested against various activities using cell line assay in their specific medium (Med). The test formulation was tested for cell viability, and the results showed that the test formulation at tested concentrations was found safe and non-toxic. Cytoprotective activity showed improved cellular restoration by 105.4% (at 25 µg/mL), 32.8% (at 25 µg/mL), and 151.8% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BTMed + BT-TI groups respectively, as compared to the untreated test group in the human cardiac fibroblasts cells (HCF) cells, while improved restoration of cell viability by 22.4% (at 25.5 µg/mL), 67.1% (at 10 µg/mL), and 72.9% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups respectively, as compared to the untreated test group in HepG2 cells. Cellular restoration in A549 cells was improved by 9.3%, 70.4%, and 14.1% at 0.1, 1, and 25.5 µg/mL respectively, in the BT-Med + UT-TI group, while 3% and 4.6% improved cellular restoration was reported at 10 and 25.5 µg/mL respectively, at BT-Med + BT-TI groups as compared to the untreated test group. ALP activity in Ishikawa cells was significantly increased by 68.4%, 41.1%, and 18.8% at 0.1, 10, and 50 µg/mL respectively, in the UT-Med + BTTI group, while in MG-63 cells showed maximum increased ALP activity by 92.7%, 84.5%, and 93.2% respectively in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI group respectively, at 50 µg/mL as compared to the untreated group. The maximum percent cellular protection of HCF (heart) cells (decreased of LDH activity) was significantlyincreased by 51.6%, 88.7%, and 53.7% in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups respectively, at 10 µg/mL as compared to the untreated group. Alanine amino transferase (ALT) activity was reported in terms of percent cellular protection of HepG2 (liver) cells. The test data showed improved HepG2 cells protection (represents decreased ALT activity) by 33%, 90.2%, and 72.1% in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups respectively, at 25 µg/mL as compared to the untreated test group. Percentage cellular protection of A549 (lungs) cells (represents increased of SOD activity) was increased by 21.5% at 25.5 µg/mL in the UT-Med + BT-TI, while 33.4%, 25.8%, and 21.5% at 1, 10, and 25.5 µg/mL respectively, in the BT-Med + UT-TI group, and 12.8% increased SOD activity at 25.5 µg/mL in the BT-Med + BT-TI groups as compared to the untreated group. Serotonin level was significantly increased 137.4% (at 0.1 µg/mL), 65.4% (at 0.1 µg/mL), and 77.3% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UTTI, and BT-Med + BT-TI groups, respectively compared to untreated test group in human neuroblastoma cells (SH-SY5Y). However, the relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 24.2% (at 1 µg/mL), 213.7% (at 10 µg/mL), and 328.7% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively as compared to the untreated in MG-63 cells. Thus, the data concluded that The Trivedi Effect® would be significantly useful for improving multiple organs health, which can be used for many coronary artery diseases, arrhythmias, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, asthma, chronic bronchitis, cystic fibrosis, osteoporosis, etc.

Biofield Energy Treatment Based Test Formulation as a Novel and Efficient Approach on Various Biomarkers in human Bones, Heart, Liver, Lungs, and Brain Cells

Multiple organ dysfunction syndromes (MODS) are one of the common reasons for increased mortality rate against healthcare services. The present experiment aimed to determine the impact of the Biofield Energy Treated test formulation on the function of vital organs such as bones, heart, liver, lungs, and brain using standard activity parameters in specific cell-based assays. The test formulation and cells medium was divided into two parts; one untreated (UT) and other part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Laura Nelson Streicher, and USA and were labeled as the Biofield Energy Treated (BT) test formulation/media. The test formulation was tested against various activities using cell line assay. The test formulation was tested for cell viability, and the results showed that the test formulation was found non-toxic against all the cell lines at the tested concentrations. Cytoprotective activity among the experimental groups showed a significant improved activity by 156.9% at 1 µg/mL in UT- medium (Med) + BT - test item/formulation (TI) group in human cardiac fibroblasts cells (HCF) cells, while 94.5% at 25.5 µg/mL in the UT-Med + BT-TI group in human hepatoma cells (HepG2), and 92.4% at 63.75 µg/mL in the BT-Med + BT-TI group as compared with the untreated test group in adenocarcinomic human alveolar basal epithelial cells (A549) cells as compared with the untreated test group. Alkaline phosphatase (ALP) activity in MG-63 cells was significantly increased by 80.2% and 93.5% at 10 and 50 µg/mL respectively in the UT-Med + BT-TI group, while in Ishikawa cells showed maximum increased ALP activity by 51.5% at 1 µg/mL in UT-Med + BT-TI group as compared to the untreated group. The percent protection of HCF (heart) cells (decreased of lactate dehydrogenase-LDH activity) was significantly increased by 156.9% and 18.3% at 1 and 10 µg/mL concentrations respectively, in UT-Med + BT-TI group, while BT-Med + UT-TI group showed increased protection by 24.5% and 17.7% at10 and 25.5 µg/mL respectively, and 67.1%, 32.2%, 47.7%, and 73.1% improved cellular protection 1, 10, 25.5, and 63.75 µg/mL respectively, in BT-Med + BT-TI group as compared to the untreated test group. Alanine amino transferase (ALT) in terms of percent protection of HepG2 (liver) cells (decreased of ALT activity) was reported by 93.1% and 64.1% at 1 µg/mL in the UT-Med + BT-TI and BT-Med + UT-TI groups, respectively as compared to the untreated group. Cellular protection of A549 (lungs) cells (increased of superoxide dismutase-SOD activity) in terms of percentage was increased by 20.6% (at 0.1 µg/mL) and 6.9% (at 10 µg/mL) in the UT-Med + BT-TI and BT-Med + BT-TI groups, respectively as compared to untreated group. Serotonin level was significantly increased by 59.3% (at 10 µg/mL), 55.6% (at 1 µg/mL), and 100.1% (at 0.1 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively compared to untreated in human neuroblastoma cells (SH-SY5Y). However, the relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 150.6% (at 50 µg/mL), 380.1% (at 10 µg/mL), and 148.1% (at 50 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively as compared to the untreated in MG-63 cells. In conclusion, test formulation would be significantly useful for multiple organ health and improve overall health of bones, heart, liver, lungs, and brain after treatment with the Biofield Energy treated test formulation (The Trivedi Effect®). As a complementary and alternative therapy, Biofield Energy approach can be used against coronary artery disease, arrhythmias, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, asthma, chronic bronchitis, cystic fibrosis, osteoporosis, etc.

Assessment of Biofield Energy Healing Based Vitamin D3 Effects on Bone Health Parameters Using Human Osteoblast Cell Line (MG-63)

Abstract Poor bone health is the primary health issue, which leads to significant health problems, stress and worsening the patients' quality of life. The potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 as a test item (TI) and DMEM on MG-63 cells was investigated. The test items were treated with The Trivedi Effect® by Mahendra Kumar Trivedi and divided as Biofield Energy Treated (BT) and untreated (UT) test items. An increase in ALP activity, collagen levels, and bone mineralization was considered as the biomarker for bone health. MTT data showed that the test samples observed nontoxic in the tested concentrations. The level of ALP was significantly increased by 832.9% and 209.4% in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively at 10 µg/mL, while 222.9% increase in the BT-DMEM+BT-TI at 1 µg/mL compared to the untreated group. Collagen was significantly increased by 487.7% and 544.5% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100 µg/mL, while 116.2% at 1 µg/mL in UT-DMEM+BT-TI compared to the untreated group. Moreover, the percent of bone mineralization was significantly increased in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups by 344.9% and 149.7%, respectively at 50 µg/mL, while 183.6% in the BT-DMEM+BT-TI group at 100 µg/mL compared to the untreated group. Thus, the role of Biofield Energy Treated vitamin D3 and DMEM in order to control osteoblast function and its direct effects on bone mineralization can be used to improve bone disorders.

"A Comparative Analysis of Vitamin D3 and Biofield Energy Treated Vitamin D3 in Bone Health Using MG-63 Cell Line"

Poor bone health is related with various bone disease that predisposes to fractures, while sufficient supplementation with calcium and vitamin D suggested prevention of bone diseases. The present study aimed to find the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on in vitro bone health parameters using MG-63 cells such as alkaline phosphates enzyme (ALP) activity, collagen levels, and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lauree Ann Duprey-Reed and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability range 76% to 139% with safe and nontoxic profile of test samples on MG-63 cell line. ALP was significantly increased by 171.8% and 189.7% at 10µg/mL in the UT-DMEM+BT-TI and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. In addition, 100% and 33.3% increased ALP at 0.1 and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. The level of collagen was significantly increased at 10µg/mL by 145.8% in the UT-DMEM+BT-TI group, while 65.8% and 145.8% at 1 and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 86.3% and 136.1% at 1 and 10µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 150%, 21%, and 73.2% at 0.1, 10, and 100µg/mL, respectively in the UT-DMEM+BT-TI group, while 258.3% and 126.8% at 0.1 and 100µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 33.3%, 32.7%, and 187.4% at 0.1, 10, and 100µg/mL, respectively as compared with the untreated group. Biofield Energy Treatment might be vital in promotion and maintenance of strong and healthy bones and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer, and many autoimmune disorders.

Biofield Energy Enriched Vitamin D3 versus Vitamin D3 in Preventing Fractures and Bone Loss Using MG-63 Cells

Bone is a dynamic tissue, which continually adapting its structure during the development process. The aim of the study was to investigate the effect of Consciousness Energy Healing based vitamin D₃ and DMEM medium on bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization using MG-63 cells. The test items (TI) i.e. vitamin D₃ and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lorraine Marie Hachfeld and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 79% with a safe and nontoxic profile on MG-63 cell line. The level of ALP was increased by 400%, 84%, and 179.4% at 0.1, 1, and 10μg/mL, respectively in the UT-DMEM+BT-TI, while 314.3% at 10μg/mL in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. BT-DMEM+BT-TI group showed an increased ALP level by 128% and 60.3% at 1 and 10μg/mL, respectively. Collagen was significantly increased by 180% and 128.1% at 0.1 and 1μg/mL, respectively in the UT-DMEM+BT-TI group, while 84.4% and 69.7% at 1 and 10μg/ mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. Furthermore, BT-DMEM+BT-TI group showed a significant increased collagen level by 123.4% and 184.8% at 1 and 10 μg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 385.1% at 0.1μg/mL in the UT-DMEM+BT-TI group, while 53.2%, 30.2%, and 163.9% at 0.1, 1, and 10μg/ mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 283%, 65.1%, and 197.3% at 0.1, 1, and 10μg/mL, respectively as compared with the untreated group. The experimental data suggested that the Biofield Energy Treated vitamin D₃ and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. It regulates the osteoblast function, improves the bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Effect of Biofield EnergyTreated Vitamin D3 on the Proliferation of Osteoblast-Like MG-63 Human Osteosarcoma Cells in vitro

Author(s): Kinney, Janice Patricia; Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Mondal, Sambhu Charan; Jana, Snehasis | Abstract: The study objective was to evaluate the effect of Biofield Treated vitamin D3 and DMEM on bone health. The Test Items (TI) were divided into two parts. One part of each sample was received Consciousness Energy Healing Treatment by Janice Patricia Kinney and those samples were labeled as Biofield Energy Treated (BT) samples, while other parts of each sample were denoted as Untreated Test items (UT). The cell viability showed test samples were found as safe in the tested concentrations. ALP was significantly increased by 212.57% and 134.55% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 50 µg/mL, while 242.77% increased in BT-DMEM + UT-TI at 10 µg/mL compared to UT-DMEM + UT-TI group. Collagen was significantly increased by 172.04%, 293.55%, and 248.39% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 0.1 µg/mL compared to untreated group. Moreover, collagen was significantly increased by 99.25% and 172.93% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 1µg/mL, while increased by 156.38% and 145.64% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 10 µg/mL than untreated group. Besides, percent of bone mineralization was distinctly increased by 198.62%, 111.72%, and 168.28% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 0.1µg/mL compared to untreated group. Overall, Biofield Treated vitamin D3 was remarkably improved the bone health parameters and could be beneficial against bone-related disorders (osteoporosis, osteomalacia etc.), stress, inflammatory and autoimmune disorders.

Biofield Energy Enriched Vitamin D<sub>3</sub>: A New Horizons for Development of Bone Health Using MG-63 Cells

The current study investigates the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Maire Anne Mayne and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 78% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 141.9% and 25% at 50 and 100 µg/mL, respectively in the UT-DMEM+BT-TI group as compared with the untreated group. In addition, 144.6% (100 µg/mL) and 120.6% (1 µg/mL) increase in ALP activity in BT-DMEM+UT-TI and BT-DMEM+BT-TI group, respectively as compared with the untreated group. The level of collagen was significantly increased by 58.7% and 72.6% at 1 and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 39.7% and 101.6% at 1 and 10 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 117.5%, 163.5%, and 169.4% at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 110.2%, 116.8%, and 81% at 10, 50, and 100 µg/mL, respectively in UT-DMEM+BT-TI group, while 148.8%, 180.1%, and 129.4% at 10, 50, and 100 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 142%, 181.1%, and 113% at 10, 50, and 100 µg/mL, respectively as compared with the untreated group. The experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might be vital in maintaining the various clinical safety and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Effect of Biofield Energized Vitamin D<sub>3</sub> on Bone Health in MG-63 Cell-Line

The objective of current research work was to evaluate the potential of Consciousness Energy Healing-based vitamin D₃ and DMEM medium on bone health parameters such as alkaline phosphatase (ALP), collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The test items like vitamin D₃ and DMEM were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Faith Ann Pyka and those samples were denoted as the Biofield Energy Treated (BT) samples, while the other parts of each sample were referred as the untreated test items (UT). The MTT cell viability assay revealed that the test samples were found as safe in the tested concentrations. The level of ALP was significantly increased by 191.45% in the BT-DMEM + UT-Test item (UT-TI) group at 1 µg/mL compared to the UT-DMEM + UT-TI group. Moreover, ALP level was significantly increased by 144.89% and 226.75% in the UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 50 µg/mL compared to the untreated group. Further, collagen level was significantly increased by 98.15%, 80.54%, and 49.98% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 10 µg/mL compared to the untreated group. Additionally, at 50 µg/mL level of collagen was significantly increased by 129.73%, 189.16%, and 94.60% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively with respect to the untreated group. Apart from this, the percent of bone mineralization was distinctly enhanced by 283.74%, 27.91%, and 118.02% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 50 µg/mL compared to the untreated group. Moreover, the percentage of bone mineralization was significantly increased by 265.92%, 231.82%, and 158.2% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 100 µg/mL compared to the untreated group. Altogether, the Biofield Energy Treated vitamin D₃ was significantly improved the bone health parameters and it could be an alternative approach for nutraceutical supplement to combat vitamin D₃ deficiency and able to fight against various bone-related disorders including rickets, low bone density, osteomalacia, bone and joint pain, bone fractures, osteoporosis, osteoma, osteogenesis imperfecta, Paget’s disease, deformed bones, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

Improved Metabolism of Vitamin D<sub>3</sub> in Human Osteoblasts Cells After Biofield Energy Healing Treatment

Bone formation, remodeling, mineralization, resorption, and healing processes involves a coordinated action of different cell types, which are very prevalent in the elderly population. The current study aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Vaibhav Rajan Parulkar and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were studied. The cell viability using MTT assay showed that the test items were found to be safe. ALP level was significantly increased by 304.6%, 237.2%, and 110.6% at 1, 10, and 50 µg/mL, respectively in BT-DMEM+UT-TI, while 356.9%, 186.2%, and 87.3% at 1, 10, and 50 µg/mL, respectively in the BT-DMEM+BT-TI group as compared to the untreated group. Collagen content was significantly increased up to 163.7%, 114.4%, and 22.7% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 190.1%, 64.9%, and 50% increased collagen at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI group as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 330% and 121.5% at 0.1 and 1 µg/mL, respectively in the BT-DMEM+BT-TI group, while 146.7% and 305.9% at 0.1 and 1 µg/mL, respectively in the BT-DMEM+UT-TI group, as compared with the untreated group. In conclusion, the Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium, and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures.

Effects of Vitamin D<sub>3</sub> on the Proliferation and Mineralization of Human Osteoblast-Like Cells: Implications of Biofield Energy Healing Treatment

Silently progressing metabolic bone disorders are widely increased that affects the quality of life as one of the key health issues today. The aim of the study is to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters. Vitamin 3 and DMEM medium as test items (TI), were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Thomas Charles Slade and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization as bone health biomarkers were studied. The cell viability (˃84%) using MTT assay showed that the test items were found to be safe. ALP level was significantly increased by 218.6% (100 µg/mL), 263.9% (10 µg/mL), and 135.7% (100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively. Collagen content was significantly increased by 147.6%, 153.1%, and 240.8% at 10, 50, and 100 µg/mL, respectively in the UT-DMEM+BT-TI group, while 150.8% and 212.9% at 50 and 100 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 104.6% and 185.9% in UT-DMEM+BT-TI and BT-DMEM+BT-TI groups, respectively at 10 µg/mL, while 119%, 42.6%, and 102.3% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. Overall, the Biofield Energy Treated vitamin 3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures.

Regulation of Bone Health Parameters in MG-63 Cell Line After Treatment with Biofield Energy Treated Vitamin D<sub>3</sub>

The present experiment aimed to explore the potential of Consciousness Energy Healing based vitamin D₃ and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) <i>i.e</i>. vitamin D₃ and DMEM medium were divided into two parts. The test items received the Consciousness Energy Healing Treatment by Matthew Charles Hornung and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of both the sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability in tested samples was more than 88% on MG-63 cell line. ALP level was significantly increased by 273.3% (at 0.1 µg/mL), 694.1% (at 1 µg/mL), and 180% (at 0.1 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 105.8% (at 1 µg/mL), 164.6% (at 0.1 µg/mL), and 193.8% (at 0.1 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Bone mineralization percentage was significantly increased by 238.1%, 92.7%, and 43.1% at 0.1, 1, and 10 µg/mL, respectively in UT-DMEM+BT-TI group, while 297.6%, 86.2%, and 66.3% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 281%, 113%, and 47% at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D₃ and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

&amp;lt;i&amp;gt;In Vitro&amp;lt;/i&amp;gt; Effects of Biofield Energy Treated Vitamin D&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; Supplementation on Bone Formation by Osteoblasts Cells

Various bone disorders including fractures, significant pain and height loss, disability to stand up, and walk occurs due to insufficient level of vitamin D and calcium in the body. Both plays an important role to improve the patients' quality of life with respect to bone disorders. The present experimental analysis designed to explore the potential of Consciousness Energy Healing based vitamin D₃ and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) <i>i.e.</i> vitamin D₃ and DMEM medium were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Sakina Aleemah Ansari and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 79% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 119% (at 50 µg/mL), 161.5% (at 1 µg/mL), and 252.3% (at 50 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 81% (at 10 µg/mL), 117.9% (at 1 µg/mL), and 134.2% (at 0.1 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. In addition, the percent of bone mineralization was significantly increased by 235.2% at 100 µg/mL in UT-DMEM+BT-TI group, while 152.7% and 92.6% at 0.1 and 1 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 158% at 1 µg/mL as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D₃ and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Exploring the Effects of Biofield Energy Treated Vitamin D&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; on Bone Health Parameters in MG-63 Cell Line

The aim of the present experiment is to define the potential of Consciousness Energy Healing based vitamin D 3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen level, and bone mineralization. Both the test items (TI) i.e. vitamin D 3 and DMEM medium were divided into two parts. The test samples were received Consciousness Energy Healing Treatment by Rolando Baptista Piedad and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability of test samples using MTT assay showed that cell viability was more than 87% with safe and nontoxic profile on MG-63 cell line. ALP level was significantly increased by 196.1% (at 10 µg/mL) and 131.6% (at 0.1 µg/mL) in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively. In addition, ALP level was significantly increased by 342.1%, 205.2%, and 289.5% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI groups as compared with the untreated test item and DMEM group. The percentage of collagen level was significantly increased by 109.5%, 67.0%, and 100.9% at 0.1, 1, and 10 µg/mL, respectively in UT-DMEM+BT-TI group, while 228.4%, 157.8%, and 154.1% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI as compared with the untreated group. The percent of bone mineralization was significantly increased by 40.8% at 0.1 µg/mL in UT-DMEM+ BT-TI group, while 59.2% at 0.1 µg/mL in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 128% and 174.1% at 50 and 100 µg/mL, respectively as compared with the untreated group. Biofield Energy Treated vitamin D 3 3 and DMEM would enhance the promotion and maintenance of strong and healthy bones. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of autoimmune diseases, adverse bone health conditions, comprising cancer, and bone disorders such as Rickets, Osteogenesis Imperfecta, Osteomalacia, Perthes' Disease, Fibrous Dysplasia, Osteomyelitis, Paget's Disease, and Osteoporosis.

Impact of Biofield Energy Healing Treated Vitamin D&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; on Human Osteoblast Cell Line (MG-63) for Bone Health

Bone disorders are largely associated with increased morbidity, mortality, and substantial economic and health costs. Vitamin D play an important role for calcium absorption and bone mineralization, which can improve the patients' quality of life. The current study aimed to evaluate the potential of The Trivedi Effect^®- Biofield Energy Healing on vitamin D₃ and DMEM as test item (TI) on bone cell differentiation using human osteoblast cell line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect^® by William Dean Plikerd and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 346.4% (at 50 µg/mL), 375.3% (at 100 µg/mL), and 343.2% (at 100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 336.2% and 237.2% in the UT-DMEM+BT-TI at 10 and 50 µg/mL, respectively while 399.3% and 110.2% increased collagen at 10 µg/mL in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively. Moreover, the percent of bone mineralization was significantly increased in BT-DMEM+BT-TI group by 71.3% and 97.2% at 50 and 100 µg/mL, respectively as compared with the untreated test item and DMEM group. Thus, Biofield Energy Treated vitamin D₃ and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.

Biofield Energy Treated Vitamin D&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;: Therapeutic Implication on Bone Health Using Osteoblasts Cells

Calcium and vitamin D are the main constituents of bone health, while insufficient level leads to various diseases such as osteoporosis causing significant morbidity and mortality. The objective of the study is to investigate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters in MG-63 cells such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. Both the sample received Consciousness Energy Healing Treatment by Mark E Stutheit and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 85% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 126.1% (at 50 µg/mL), 128.2% (at 50 µg/mL), and 173.7% (at 100 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 104.3% (at 10 µg/mL), 104.3% (at 50 µg/mL), and 97.4% (at 50 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. In addition, the percent of bone mineralization was significantly increased by 82.4%, 118.4%, and 202.4% at 0.1, 1, and 10 µg/mL, respectively in UT-DMEM+BT-TI group, while 158.8%, 197.4%, and 200% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 52.9%, 131.6%, and 235.7% at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might be vital in maintaining the various clinical safety and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Biofield Energy Treated Vitamin D&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;: Direct Effect on Bone Health Using Human Osteoblast-Like Cells

Vitamin D and calcium are one of the important constituents of good bone health. Bone disorders might be to the hormonal factors and lack of proper nutrition that leads to gradual bone loss. The current study aimed to explore the potential of Consciousness Energy Healing based vitamin D₃ and DMEM medium on various bone health parameters. Vitamin D₃ and DMEM medium as test items (TI), were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Sheila Maureen Benson and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were measured. MTT data showed that cell viability was more than 70% and all the test items were found to be safe at the tested concentrations. ALP level was significantly increased by 135.9% and 134.5% at 1 and 50 µg/mL, respectively in the UT-DMEM+BT-TI, while 84.4%, 93.8%, and 50.0% at 1, 50, and 100 µg/mL, respectively in BT-DMEM+UT-TI group. In addition, BT-DMEM+BT-TI group showed increased ALP by 50.2% and 104.7% at 10 and 50 µg/mL, respectively as compared with the untreated test item and DMEM group. Collagen content was significantly increased by 463.6%, 387.9%, and 239.4% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI group, respectively at 0.1 µg/mL as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 131.7% and 81.7% at 1 and 10µg/mL, respectively in UT-DMEM+BT-TI group, while 83.1% and 53.6% at 10 and 50µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 152.3% and 108.3% at 50 and 100µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D₃ and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Regulation of Bone Health Parameters After Treatment with Biofield Energy Healing Based Vitamin D&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; on Human Osteoblast Cell Line (MG-63)

Bone disorders dramatically affecting the functional status of many individuals, which are suffering from bone diseases such as fractures, significant pain and height loss, disability to stand up, and walk. Vitamin D play an important role to improve the patients' quality of life with respect to bone disorders. The current study aimed to evaluate the potential of The Trivedi Effect^®- Biofield Energy Healing on vitamin D₃ and DMEM as test item (TI) on bone cell differentiation using human osteoblast cell line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect® by Victoria Lee Vannes and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 114.3%, 304.8%, and 314.3% at 0.1 µg/mL in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 82.5%, 138.4%, and 100.8% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI, while 120.6% and 64.6% increased collagen at 0.1 and 1 µg/mL in BT-DMEM+UT-TI group and 261.9%, 179.8%, and 116.0% increased collagen in BT-DMEM+BT-TI group at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 261.2% and 239.9% at 1 µg/mL UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively as compared with the untreated group. However, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 324.5% at 50 µg/mL. Thus, Biofield Energy Treated vitamin D₃ and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D₃ could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.

Implication of Biofield Energy Healing Based Vitamin D&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt; on Osteoblastic Differentiation

Bone is one of the dynamic organ of the endoskeleton, which play a vital role in maintain the structural integrity, blood production, coagulation, mineral reservoirs, and body immunity. The present study investigates the potential of Consciousness Energy Healing based vitamin D₃ and DMEM medium on bone health parameters in MG-63 cell line. Vitamin D₃ and DMEM medium as test items (TI), were divided into two parts, and each sample received the Consciousness Energy Healing Treatment by Su-Mei Chen Liu and labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization as bone biomarkers were studied. The cell viability using MTT assay showed more than 84% in test items groups were found to be safe. ALP level was significantly increased by 217% (100 µg/mL), 272.8% (10 µg/mL), and 233.9% (100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively. Collagen content was significantly increased by 121.6%, 134.3%, and 86% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 100 µg/mL as compared with the untreated group. Additionally, the percent of bone mineralization was significantly increased by 213.5% (at 10 µg/mL), 109.5% (at 100 µg/mL), and 283.7% (at 100 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. In conclusion, the Biofield Energy Treated vitamin D₃ and DMEM would play an important role to improve the bone mineralization and calcium absorption. The bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Further, it is assumed that the Biofield Energy Treated vitamin D₃ could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss, and fractures.