Biofield Energy Treated Vitamin D&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;: Direct Effect on Bone Health Using Human Osteoblast-Like Cells

Sheila Maureen Benson

doi:10.11648/j.cb.20180601.11

Abstract

Vitamin D and calcium are one of the important constituents of good bone health. Bone disorders might be to the hormonal factors and lack of proper nutrition that leads to gradual bone loss. The current study aimed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Sheila Maureen Benson and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were measured. MTT data showed that cell viability was more than 70% and all the test items were found to be safe at the tested concentrations. ALP level was significantly increased by 135.9% and 134.5% at 1 and 50 µg/mL, respectively in the UT-DMEM+BT-TI, while 84.4%, 93.8%, and 50.0% at 1, 50, and 100 µg/mL, respectively in BT-DMEM+UT-TI group. In addition, BT-DMEM+BT-TI group showed increased ALP by 50.2% and 104.7% at 10 and 50 µg/mL, respectively as compared with the untreated test item and DMEM group. Collagen content was significantly increased by 463.6%, 387.9%, and 239.4% in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI group, respectively at 0.1 µg/mL as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 131.7% and 81.7% at 1 and 10µg/mL, respectively in UT-DMEM+BT-TI group, while 83.1% and 53.6% at 10 and 50µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 152.3% and 108.3% at 50 and 100µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Highlights

Vitamin D has multiple effects which regulate the functions in different organs such as brain, lungs, liver, kidneys, and heart, immune, skeletal, and reproductive systems
The level of collagen was significantly increased by 463.6%, 55.7%, and 41.2% at 0.1, 1, and 10μg/mL, respectively in untreated test items (UT)-Dulbecco's Modified Eagle's Medium (DMEM)+Biofield Energy Treated (BT)-test items (TI), while 387.9% at 0.1μg/mL in BT-DMEM+Untreated-Test item (UT-TI) group
The bone mineralization percent was significantly increased by 131.7%, 81.7%, and 37.9% at 1, 10, and 50μg/mL, respectively in UT-DMEM+Biofield Energy Treated test item (BT-TI) group, while 83.1%, 53.6%, and 37.4% at 10, 50, and 100μg/mL, respectively in BTDMEM+UT-TI group as compared with the untreated group

Summary

Introduction

Vitamin D has multiple effects which regulate the functions in different organs such as brain, lungs, liver, kidneys, and heart, immune, skeletal, and reproductive systems. It has significant anti-inflammatory, antiarthritic, anti-osteoporosis, anti-stress, anti-aging, antiapoptotic, wound healing, anti-cancer, anti-psychotic, and anti-fibrotic roles. Calcitriol play an important role in maintaining the normal level of calcium and phosphorus, promotes bone mineralization, induce or repress the genes responsible for conserving the mineral homeostasis and skeletal integrity, and inhibit hypertension, kidney damage, cardiovascular and immune disorders (such as Lupus, Addison Disease, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Anemia, Sjogren Syndrome, Systemic Lupus Erythematosus, Diabetes, Alopecia Areata, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis), and the secondary hyperparathyroidism [3]. In order to avoid the bone related disorders such as osteomalacia, exacerbate osteoporosis, hyperparathyroidism, immune disorders, etc. calcium 1000-1500 mg/day along with vitamin D supplement around 400 IU/day is very important for maintaining the good bone health [5]

Methods

Results

Conclusion