Biofield Energy Treated Vitamin D&amp;lt;sub&amp;gt;3&amp;lt;/sub&amp;gt;: Therapeutic Implication on Bone Health Using Osteoblasts Cells

Mark E Stutheit

doi:10.11648/j.ajls.20180601.13

Abstract

Calcium and vitamin D are the main constituents of bone health, while insufficient level leads to various diseases such as osteoporosis causing significant morbidity and mortality. The objective of the study is to investigate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters in MG-63 cells such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. Both the sample received Consciousness Energy Healing Treatment by Mark E Stutheit and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 85% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 126.1% (at 50 µg/mL), 128.2% (at 50 µg/mL), and 173.7% (at 100 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 104.3% (at 10 µg/mL), 104.3% (at 50 µg/mL), and 97.4% (at 50 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. In addition, the percent of bone mineralization was significantly increased by 82.4%, 118.4%, and 202.4% at 0.1, 1, and 10 µg/mL, respectively in UT-DMEM+BT-TI group, while 158.8%, 197.4%, and 200% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 52.9%, 131.6%, and 235.7% at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might be vital in maintaining the various clinical safety and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Highlights

Vitamin D has multiple effects which regulate the functions in different organs such as brain, lungs, liver, kidneys, and heart, immune, skeletal, and reproductive systems
The bone health parameters such as the level of Alkaline Phosphatase (ALP) was increased by 126.1% and 11.2% at 50 and 100 μg/mL, respectively in the UTDMEM+Biofield Energy Treated (BT)-test items (TI), while 128.2% at 50 μg/mL in the BTDMEM+untreated test items (UT)-TI group as compared with the untreated test item and Dulbecco's Modified Eagle's Medium (DMEM) group
The level of collagen was significantly increased by 24.5% and 104.3% at 1 and 10 μg/mL, respectively in the UT-DMEM+Biofield Energy Treated test item (BT-TI), while 45.3% and 104.3% at 10 and 50 μg/mL, respectively in the BT-DMEM+Untreated-Test item (UT-TI) group

Summary

Introduction

Vitamin D has multiple effects which regulate the functions in different organs such as brain, lungs, liver, kidneys, and heart, immune, skeletal, and reproductive systems. It has significant anti-inflammatory, anti-arthritic, anti-osteoporosis, anti-stress, anti-aging, antiapoptotic, wound healing, anti-cancer, anti-psychotic, and anti-fibrotic roles. Calcitriol play an important role in maintaining the normal level of calcium and phosphorus, promotes bone mineralization, induce or repress the genes responsible for conserving the mineral homeostasis and skeletal integrity, and inhibit hypertension, kidney damage, cardiovascular and immune disorders (such as Lupus, Addison Disease, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Anemia, Sjogren Syndrome, Systemic Lupus Erythematosus, Diabetes, Alopecia Areata, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis), and the secondary hyperparathyroidism [3]. In order to avoid the bone related disorders such as osteomalacia, exacerbate osteoporosis, hyperparathyroidism, immune disorders, etc. calcium 1000-1500 mg/day along with vitamin D supplement around 400 IU/day is very important for maintaining the good bone health [5]

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Conclusion