Exploring the Effects of Biofield Energy Treated Vitamin D&amp;lt;sup&amp;gt;3&amp;lt;/sup&amp;gt; on Bone Health Parameters in MG-63 Cell Line

Rolando Baptista Piedad

doi:10.11648/j.ijfsb.20180301.13

Abstract

The aim of the present experiment is to define the potential of Consciousness Energy Healing based vitamin D 3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen level, and bone mineralization. Both the test items (TI) i.e. vitamin D 3 and DMEM medium were divided into two parts. The test samples were received Consciousness Energy Healing Treatment by Rolando Baptista Piedad and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability of test samples using MTT assay showed that cell viability was more than 87% with safe and nontoxic profile on MG-63 cell line. ALP level was significantly increased by 196.1% (at 10 µg/mL) and 131.6% (at 0.1 µg/mL) in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively. In addition, ALP level was significantly increased by 342.1%, 205.2%, and 289.5% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI groups as compared with the untreated test item and DMEM group. The percentage of collagen level was significantly increased by 109.5%, 67.0%, and 100.9% at 0.1, 1, and 10 µg/mL, respectively in UT-DMEM+BT-TI group, while 228.4%, 157.8%, and 154.1% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI as compared with the untreated group. The percent of bone mineralization was significantly increased by 40.8% at 0.1 µg/mL in UT-DMEM+ BT-TI group, while 59.2% at 0.1 µg/mL in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 128% and 174.1% at 50 and 100 µg/mL, respectively as compared with the untreated group. Biofield Energy Treated vitamin D 3 3 and DMEM would enhance the promotion and maintenance of strong and healthy bones. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of autoimmune diseases, adverse bone health conditions, comprising cancer, and bone disorders such as Rickets, Osteogenesis Imperfecta, Osteomalacia, Perthes' Disease, Fibrous Dysplasia, Osteomyelitis, Paget's Disease, and Osteoporosis.

Highlights

Vitamin D has multiple effects which regulate the functions in different organs such as brain, lungs, liver, kidneys, and heart, immune, skeletal, and reproductive systems
Collagen level was significantly increased by 109.5%, 67.0%, and 100.9% at 0.1, 1, and 10 μg/mL, respectively in the untreated test items (UT)-DMEM+Biofield Energy Treated (BT)-test items (TI), while 228.4%, 157.8%, and 154.1% at 0.1, 1, and 10 μg/mL, respectively in the BT-DMEM+Untreated-Test item (UT-TI) group
The bone mineralization percent was significantly increased by 40.8%, 28%, and 28.1% at 0.1, 50, and 100 μg/mL, respectively in the UTDMEM+Biofield Energy Treated test item (BT-TI) group, while 59.2%, 12.6%, and 10.5% at 0.1, 10, and 100 μg/mL, respectively, in the BT-DMEM+UTTI group as compared with the untreated group

Summary

Introduction

Vitamin D has multiple effects which regulate the functions in different organs such as brain, lungs, liver, kidneys, and heart, immune, skeletal, and reproductive systems. It has significant anti-inflammatory, antiarthritic, anti-osteoporosis, anti-stress, anti-aging, antiapoptotic, wound healing, anti-cancer, anti-psychotic, and anti-fibrotic roles. Calcitriol play an important role in maintaining the normal level of calcium and phosphorus, promotes bone mineralization, induce or repress the genes responsible for conserving the mineral homeostasis and skeletal integrity, and inhibit hypertension, kidney damage, cardiovascular and immune disorders (such as Lupus, Addison Disease, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Anemia, Sjogren Syndrome, Systemic Lupus Erythematosus, Diabetes, Alopecia Areata, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis), and the secondary hyperparathyroidism [3]. In order to avoid the bone related disorders such as osteomalacia, exacerbate osteoporosis, hyperparathyroidism, immune disorders, etc. calcium 1000-1500 mg/day along with vitamin D supplement around 400 IU/day is very important for maintaining the good bone health [5]

Methods

Results

Conclusion