Brown Rot Research Articles

Characterizing properties of fungal-decayed cross laminated timber (CLT) connection assemblies

The effects of brown rot decay fungi on mechanical properties of CLT connection assemblies were investigated. CLT connections evaluated were assembled with code approved angle bracket connectors and modelled as floor-to-wall systems in mass timber buildings. Physical changes, mass loss and quasi-static cyclic tests were used to assess the performance of connection assemblies up to 40 weeks after fungal inoculation. Peak load, stiffness, energy dissipation and ductility of connections were characterized based on force–displacement data generated from the destructive test of connections. Assemblies experienced up to 57 % loss in load carrying capacity and 90 % loss in energy dissipating capacity of the connections after 40 weeks of fungal exposure. Connection stiffness was only slightly impacted over this period but wetting and redrying caused significant degradation of connection ductility.

Occurrence and Chemical Control Strategy of Wheat Brown Foot Rot Caused by Microdochium majus.

Wheat brown foot rot (WBFR), caused by a variety of phytopathogenic fungi, is an important soilborne and seedborne disease of wheat. WBFR causes wheat lodging and seedling dieback, which seriously affect the yield and quality of wheat. In this study, 64 isolates of WBFR were isolated from different wheat fields in Yancheng city, Jiangsu Province, China. The internal transcribed spacer, elongation factor 1α, and RNA polymerase II subunit were amplified and the sequencing results of the fragments were analyzed with BLAST in NCBI. Through morphological and molecular identification, all of the isolates were identified as Microdochium majus. Verification by Koch's postulates confirmed that M. majus was the pathogen causing WBFR. The antifungal activities of fludioxonil and prochloraz against 64 isolates of M. majus were determined based on mycelial growth inhibition method. The results showed that fludioxonil and prochloraz had good antifungal activity against M. majus. The mean 50% effective concentration values of fludioxonil and prochloraz against M. majus were 0.2956 ± 0.1285 μg/ml and 0.0422 ± 0.0157 μg/ml, respectively. Control efficacy for seed-coating treatments conducted in a greenhouse indicated that M. majus severely damaged the normal growth of wheat, while seed coating with fludioxonil or prochloraz significantly reduced the disease incidence and improved the seedling survival rates. At fludioxonil doses of 7.5 g per 100 kg and prochloraz doses of 15 g per 100 kg, the incidence was reduced by 22.26 and 25.33%, seedling survival rates increased by 25.37 and 22.66%, and control efficacy reached 70.02 and 72.30%, respectively. These findings provide vital information for the accurate diagnosis and effective management of WBFR.

Complete mitochondrial genome sequence of the Agaricomycetes brown rot fungus Fomitopsis pinicola isolate FBCC1181.

The mitochondrial genome of the brown rot fungus Fomitopsis pinicola isolate FBCC 1181 is a 66.5 kbp circular chromosome. It contains 64 predicted genes, including a set typical for Basidiomycota Agaricomycetes mitogenomes. Introns of cox and cob genes contain several homing endonucleases of both LAGLIDADG and GIY-YIG types.

Mechanical, physical and compositional effects of Meruliporia incrassata on Southern Yellow Pine

Abstract With the growing risk of fungal degradation in timber-framed structures from significant moisture intrusion events due to climate change, it is increasingly critical to develop mechanistic relationships between fungal degradation mechanisms and the strength of untreated wood components. While extensive work has been performed characterizing wood decay, no study has yet addressed the effects of Meruliporia incrassata on untreated Southern Yellow Pine. This seeks to address this knowledge gap by evaluating the effects of the common brown rot fungus on an ubiquitous building material in the southeast United States – Southern Yellow Pine. Properties of Southern Yellow Pine were evaluated at seven decay stages over the course of 12 weeks of exposure to M. incrassata. Changes in physical properties – mass, density, and moisture content – were measured at each stage. Changes in stiffness were characterized via ultrasonic pulse velocity testing, and thermogravimetric analysis was utilized to assess compositional changes. The study found rapid and significant losses in stiffness at decay stages as early as four-weeks. Hemicellulose and cellulose degradation occurred steadily throughout the decay period. These results can be utilized to develop a stronger understanding of the mechanical behavior of timber-framed structures in the United States degraded by brown rot.

Kemampuan Dekolorisasi Zat Pewarna Sintetis Menggunakan Jamur Aspergillus niger : Review

Waste from the textile industry is dominated by synthetic dyes. Synthetic dyes are complex organic components that are quite resistant to environmental conditions so they are very damaging to ecological systems. The decolorization process can be carried out with the help of biological agents. One biological agent that also has potential for the decolorization process of synthetic dyes is the brown rot fungus, Aspergillus niger. The information and literature review explained in this article refers to the latest research regarding the potential decolorization of synthetic dyes by the fungus Aspergillus niger. The literature review was carried out by analyzing qualitative data, making observations, and drawing conclusions from research journals. The results of the study show that the Aspergillus niger fungus has quite good potential, even the percent removal of synthetic dyes can reach ± ​​90% through biodegradation and biosorption processes that work simultaneously. There are several factors that influence the removal mechanism, such as: pH, agitation conditions, temperature, initial dye concentration, and nutrient composition.

Development of Glycine max Germplasm Highly Resistant to Sclerotinia sclerotiorum

Sclerotinia stem rot (SSR) of soybean caused by Sclerotinia sclerotiorum is a devastating disease of soybean, especially in the Upper Midwest region of the United States. To mitigate yield losses due to this disease, many control methods are available for producers, including cultural control practices, chemical control, and cultivars with quantitative resistance. However, due to there being few commercial cultivars with high levels of resistance, producers are often limited in their seed selection. The aim of this study was to develop novel conventional soybean cultivars with high levels of resistance to SSR, favorable agronomic traits, and resistance to additional economically important diseases. Initial crosses were conducted in 2016 with two different sources of SSR resistance. Across multiple generations of screening for resistance to SSR, three highly resistant soybean lines were identified as the elite lines. These elite lines were demonstrated to be highly resistant across multiple years in both greenhouse and field trials, including high levels of resistance to multiple diverse S. sclerotiorum isolates. The three selected elite lines also resulted in moderately high yields and favorable agronomic traits, such as low lodging and moderate branching, indicating their viability to be released for production. In addition to SSR resistance, these three elite lines demonstrated resistance to other economically important soybean diseases, such as frogeye leaf spot, anthracnose, Cercospora leaf blight, and brown stem rot. Overall, this work has led to three SSR-resistant soybean lines that could be useful for future breeding efforts or commercial soybean production. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Genome-wide association analysis of Monilinia fructicola lesion in a collection of Spanish peach landraces

Brown rot, caused by the Monilinia spp., is the disease that causes the greatest losses in stone fruit worldwide. Currently, M. fructicola has become the dominant species in the main peach production area in Spain. The fruit cuticle is the first barrier of protection against external aggressions and may have a key role in the susceptibility to brown rot. However, information on the role of skin fruit on the resistance to brown rot in peach is scarce. Previous genetic analyses in peach have demonstrated that brown rot resistance is a complex and quantitative trait in which different fruit parts and resistance mechanisms are involved. To search for genomic areas involved in the control of the cultivar susceptibility to brown rot and to elucidate the role of fruit skin against this infection, we have studied, for two consecutive seasons (2019 and 2020), the fruit susceptibility to M. fructicola, together with fruit cuticle thickness (CT) and density (CD), in a collection of 80 Spanish and 5 foreign peach cultivars from the National Peach Collection at CITA (Zaragoza, Spain). Brown rot incidence, lesion diameter, and severity index were calculated after 5 days of inoculation on non-wounded fruit. The peach collection has also been genotyped using the new peach SNP chip (9 + 9K). Genotypic and phenotypic data have been used to perform a genome-wide association analysis (GWAS). Phenotyping has shown a wide variability on the brown rot susceptibility within the Spanish germplasm as well as on CD and CT. The GWAS results have identified several significant SNPs associated with disease severity index (DSI), CD, and CT, five of which were considered as reliable SNP-trait associations. A wide protein network analysis, using 127 genes within the regions of the reliable SNPs and previously identified candidate genes (169) associated with Monilinia spp. resistance, highlighted several genes involved in classical hypersensitive response (HR), genes related to wax layers as ceramidases and lignin precursors catalyzers, and a possible role of autophagy during brown rot infection. This work adds relevant information on the complexity resistance mechanisms to brown rot infection in peach fruits and the genetics behind them.

Correction: The impact of biological soil disinfestation in conjunction with antagonists and organic materials on potato brown rot control

Correction: The impact of biological soil disinfestation in conjunction with antagonists and organic materials on potato brown rot control

A review of bacterial diseases of rice and its management in Malaysia

Rice (Oryza sativa L.) is one of the staple foods in the world including Malaysia. Rice production has been affected due to disease infections that is usually destructive and cause many losses to rice productivity. The common bacterial diseases listed in Malaysia include bacterial leaf blight (BLB) (Xanthomonas oryzae pv. oryzae), bacterial leaf streak (BLS) (Xanthomonas oryzae pv. oryzicola), sheath brown rot (SBR) (Pseudomonas fuscovaginae) and bacterial panicle blight (BPB) (Burkholderia glumae). Currently, there is less information on the bacterial disease occurrence of rice in Malaysia. Hence, this study aimed to discuss the current report on the severity and the economic losses of rice production due to BLB, BLS, SBR and BPB in Malaysia and the possible management strategies to control the disease that consequently reduce the losses. In general, management strategies for bacterial diseases are challenging due to the lack of suitable chemical control that is not harmful to the environment and human health. Furthermore, other management strategies can be used to control these diseases such as cultural control and biological control. There is a need for the control method to be upgraded to reduce the occurrence of these diseases. All the information provided in this study may contribute as a useful tool for identification of the bacterial disease in paddy fields and as a source of effective strategies to control the disease before it becomes worse.

Insights into the Isolation, Identification, and Biological Characterization Analysis of and Novel Control Strategies for Diaporthe passiflorae in Postharvest Passion Fruit.

Postharvest diseases seriously restrict developments in the passion fruit industry. In this study, we aimed to identify the postharvest pathogen affecting passion fruit, investigate its pathogenicity, and explore relevant control methods. The pathogen was isolated from rotting passion fruit and identified using morphological characteristics, ITS sequences, and phylogenetic tree analyses. Additionally, preliminary studies were conducted to assess the biological characteristics of the pathogen and evaluate the efficacy of various treatments for disease control. The fungus on the passion fruit called B4 was identified as Diaporthe passiflorae. Optimal conditions for mycelial growth were observed at 25-30 °C and pH 5-6, with starch as the carbon source and peptone as the nitrogen source. Infection by D. passiflorae accelerated fruit decay, reduced the h° value of the peel, and increased the peel cell membrane permeability when compared to the control. Notably, treatments with appropriate concentrations of ɛ-poly-l-lysine, salicylic acid, and melatonin showed inhibitory effects on the pathogen's growth in vitro and may thus be potential postharvest treatments for controlling brown rot caused by D. passiflorae in passion fruit. The results provide a scientific basis for the development of strategies to control postharvest decay and extend the storage period of passion fruit.

Control of Brown Rot Produced by Monilinia fructicola in Peaches Using a Full-Spectrum Extract of Zuccagnia punctata Cav.

Brown rot of stone fruit, caused by Monilinia spp., is one of the most important diseases worldwide, causing significant production losses. Currently, the standard practices for controlling this infection consist of repetitive use of synthetic fungicides. The global tendency encourages the demand for high-quality food products harmless to health and the environment, leading to a reduction in the use of these types of substances. Zuccagnia punctata (Fabaceae) is a perennial shrub extensively used for the treatment of fungal and bacterial infections in Argentinean traditional medicine. In this study, we isolated and characterized (morphologically and molecularly) a pathogenic and virulent strain of Monilinia fructicola, which is the most hostile species of the genus. Consequently, we explored the in vitro antifungal activity of the ethanolic extract of Z. punctata against this phytopathogen. The chalcones 2′,4′-dihydroxy-3′-methoxychalcone and 2′,4′-dihydroxychalcone were isolated from the extract and evaluated against M. fructicola demonstrating that they were responsible for this activity. To promote full-spectrum extract rather than pure compounds, we performed ex-vivo assays using fresh peaches inoculated with the pathogen, and then treated by immersion in an extract solution of 250 µg/mL concentration. Treatment with Z. punctata extract did not show a statistically significant difference from commercial fungicides in the control of fruit rot. In addition, Huh7 cell cytotoxicity evaluation showed that Z. punctata extract was less cytotoxic than commercial fungicides at the assayed concentrations. Based on our research, this plant extract could potentially offer a safer alternative to commercial fungicides for treating peach brown rot.

Potential of algal-based products for the management of potato brown rot disease

BackgroundRalstonia solanacearum causes potato brown rot disease, resulting in lower crop’s production and quality. A sustainable and eco-friendly method for controlling the disease is required. Algae’s bioactive chemicals have shown the potential to enhance plant defenses. For the first time, the efficacy of foliar application of Acanthophora spicifera and Spirulina platensis seaweed extracts, along with the utilization of dried algal biomasses (DABs) of Turbinaria ornata and a mixture of Caulerpa racemosa and Cystoseira myrica (1:1)on potato yield and brown rot suppression were investigated under field conditions. Field experiments were conducted in three locations: Location 1 (Kafr Shukr district, Kaliobeya governorate), Location 2 (Moneira district, Kaliobeya governorate), and Location 3 (Talia district, Minufyia governorate). Locations 1 and 2 were naturally infested with the pathogen, while location 3 was not. The study evaluated potato yield, plant nutritive status and antioxidants, soil available nitrogen-phosphorus-potassium (N-P-K), and organic matter percentage. Additionally, the shift in soil microbial diversity related to R. solanacearum suppression was examined for the most effective treatment.ResultsThe results revealed that seaweed extracts significantly increased potato yield at all locations, which correlated with higher phosphorus absorption, while T. ornate DAB increased potato yield only at location 2, accompanied by noticeable increases in soil nitrogen and plant phosphorus. The mixed DABs of C. racemosa and C. myrica demonstrated greater disease suppression than foliar applications. The disease-suppressive effect of the mixed DABs was accompanied by significant increases in flavonoids and total antioxidant capacity (TAC). Moreover, the application of mixed DABs increased soil bacterial biodiversity, with a higher abundance of oligotrophic marine bacterial species such as Sphingopyxis alaskensis and growth-promoting species like Glutamicibacter arilaitensis, Promicromonospora sp., and Paenarthrobacter nitroguajacolicus in all three locations compared to the untreated control. Klebsiella sp., Pseudomonas putida, and P. brassicacearum abundances were increased by the mixed DABs in Location 1. These species were less abundant in locations 2 and 3, where Streptomyces sp., Bacillus sp., and Sphingobium vermicomposti were prevalent.ConclusionsThe results demonstrated that the used seaweed extracts improved potato yield and phosphorous absorption, while the mixed DABs potentially contributed in disease suppression and improved soil microbial diversity.

Synergistic mechanism of Bacillus subtilis Czk1 combined with propiconazole and tebuconazole mixtures against Pyrrhoderma noxium

BackgroundBrown root rot disease is one of the devastating diseases in the rubber production process. It is not easy to be detected in the early stage of the disease. Our early research revealed that the rubber tree brown root rot fungus Pyrrhoderma noxium Pn006 was effectively inhibited by Bacillus subtilis Czk1 and 25% propiconazole–tebuconazole, and that the two agents might work in synergy. Therefore, in this investigation, we used non-targeted metabolomic method to evaluate the synergistic mechanism of B. subtilis Czk1 and 25% propiconazole–tebuconazole on Pyrrhoderma noxium Pn006.ResultsMetabolomics analysis identified 708 unique metabolic markers, including mainly lipids and lipid-like molecules, organic acids, alcohols, ketones, alkaloids and their derivatives, nucleotides and their analogues, benzene ring compounds and amino acids and their derivatives. Further screening identified 105 key metabolic markers that could be potential biomarkers to reveal the mechanism of biocontrol bacteria and chemical fungicides combination synergy. Three key metabolic pathways were found in pathway enrichment, including linolenic acid metabolism, benzoic acid degradation, and valine, leucine and isoleucine degradation.ConclusionsThe mechanism might be related to the influence of the energy supply and cell functional integrity of pathogenic fungal cells.Critical relevance statement A metabolomic method was utilized to investigate the synergistic effects of B. subtilis Czk1 and 25% propiconazole–tebuconazole use on Pyrrhoderma noxium Pn006.Graphical

Evaluation of dowel bearing strength of fungal-decayed cross-laminated timber

ABSTRACT Dowel bearing strength of three cross-laminated timber (CLT) species (Douglas-fir, Norway spruce, spruce pine fir) were periodically assessed through 40 weeks of exposure to two brown rot fungi species (Gloeophyllum trabeum and Rhodonia placenta). Time-dependent regression models were developed to describe the relationship between level of fungal exposure and dowel bearing strength of CLT. Obtained dowel bearing strength values were used to predict the capacity of floor-to-wall CLT connection systems. Predicted strength values were compared with capacity of actual connection systems which were subjected to similar biodeterioration treatments. Significant reductions in the dowel bearing strength of CLT were observed 30 weeks after fungal inoculation. Similar patterns of degradation were observed for both fungi in all three CLT species, but R. placenta was slightly more aggressive than G. trabeum, causing a 16.2 MPa reduction in dowel bearing strength of Douglas-fir after 40 weeks of exposure. Connection capacities estimated using National Design Specification yield models for dowel type fasteners and the dowel bearing strength of fungal-damaged CLT were consistent with observed properties and were within 85% of actual capacity of connections tested, especially in early stages of decay.

Low frequency of resistance to thiophanate-methyl in Monilinia fructicola populations from southeastern United States peach orchards

Methyl benzimidazole carbamate (MBC) fungicides were once widely used for brown rot (Monilinia fructicola) control of peach (Prunus persica (L.) Batsch) in the southeastern US, but their use was substantially reduced and often eliminated due to widespread resistance. In this study, 233 M. fructicola isolates were collected from major peach production areas in Alabama, Georgia, and South Carolina, and sensitivity to thiophanate-methyl was examined. Isolates were also collected from one organic and two experimental peach orchards. A discriminatory dose of 1 μg/ml was used to distinguish sensitive (S) and moderately sensitive (S-LR) isolates from low resistant phenotypes, while 50 and 500 μg/ml thiophanate-methyl concentrations were used to determine high resistant (HR) phenotypes. Sequence analyses were performed to identify mutations in the β-tubulin target gene and detached fruit assays were performed to determine the efficacy of a commercial product against isolates representing each phenotype. Results indicated 55.7%, 63.5%, and 75.9% of isolates from Alabama, Georgia, and South Carolina, respectively, were S to thiophanate-methyl; 44.3%, 36.5%, and 21.4% were S-LR; no isolates were LR; and only 3 isolates (1.3%) from South Carolina were HR. No mutations in S or S-LR isolates were found, but HR isolates revealed the E198A mutation, an amino acid change of glutamic acid to alanine conferring high resistance. The high label rate of a commercial product containing thiophanate-methyl controlled brown rot caused by S and S-LR isolates in detached fruit studies but was ineffective against HR isolates. The combinations of thiophanate-methyl with azoxystrobin or isofetamid, when mixed together and applied in an experimental orchard 14 days preharvest, significantly reduced brown rot incidence on pre and postharvest commercially ripe fruit and efficacy was comparable to that of a grower standard fungicide. These results indicate that thiophanate-methyl may again be useful to peach growers in the southeastern US for brown rot and fungicide resistance management.

Antifungal activity of volatile organic compounds from essential oils against the postharvest pathogens Botrytis cinerea, Monilinia fructicola, Monilinia fructigena, and Monilinia laxa.

Gray mold and brown rot, caused respectively by Botrytis cinerea and Monilinia spp., are fungal diseases responsible for significant losses during the storage of fruit and vegetables. Nowadays, the control of postharvest diseases is shifting towards more sustainable strategies, including the use of plant secondary metabolites. In this study, the antifungal activity of Origanum vulgare, Thymus vulgaris, Thymus serpyllum, Melaleuca alternifolia, Lavandula officinalis, Lavandula hybrida, Citrus bergamia, Rosmarinus officinalis, Cinnamomum zeylanicum essential oils (EOs) in vapor phase was tested in vitro against B. cinerea, Monilinia fructicola, Monilinia fructigena, and Monilinia laxa. For the experiments, a protocol using a volatile organic compounds (VOC) chamber was designed. Results indicate a dose-dependent inhibitory activity of all the tested EOs, with O. vulgare, T. vulgaris, and T. serpyllum being the most active ones, with minimum inhibitory concentrations (MIC) of 22.73, 45.45, and 22.73 µl/L, respectively, against B. cinerea and a range between 5.64 and 22.73 µl/L against the three Monilinia spp. Overall, B. cinerea presented lower sensitivity to vapor-phase EOs than any of the Monilinia strains, except for the C. zeylanicum EO, which consistently showed higher inhibition against B. cinerea. Among the three Monilinia spp., M. fructicola was the least sensitive, while M. fructigena was the most sensitive. The use of VOC chambers proved to be a reliable protocol for the assessment of antimicrobial activities of EOs. These results suggest that the VOC emitted by the tested EOs are effective towards important decay-causing fungi, and that they could be used for the control of gray mold and brown rot in in vivo trials.

Monilinia fructicola Response to White Light.

Light represents a powerful signal for the regulation of virulence in many microbial pathogens. Monilinia fructicola is the most virulent species causing brown rot in stone fruit crops. To understand the influence of light on M. fructicola, we measured the effect of white light and photoperiods on the colonial growth and sporulation of the model M. fructicola strain 38C on solid cultures. Searches in the M. fructicola 38C genome predicted a complete set of genes coding for photoreceptors possibly involved in the perception of all ranges of wavelengths. Since white light had an obvious negative effect on vegetative growth and the asexual development of M. fructicola 38C on potato dextrose agar, we studied how light influences photoresponse genes in M. fructicola during early peach infection and in liquid culture. The transcriptomes were analyzed in "Red Jim" nectarines infected by M. fructicola 38C and subjected to light pulses for 5 min and 14 h after 24 h of incubation in darkness. Specific light-induced genes were identified. Among these, we confirmed in samples from infected fruit or synthetic media that blue light photoreceptor vvd1 was among the highest expressed genes. An unknown gene, far1, coding for a small protein conserved in many families of Ascomycota phylum, was also highly induced by light. In contrast, a range of well-known photoreceptors displayed a low transcriptional response to light in M. fructicola from nectarines but not on the pathogen mycelium growing in liquid culture media for 6 days.

Molecular and Morphological Identification of Monilia yunnanensis Causing Brown Rot on Chinese Quince and Peach in Yunnan, China.

More than 30% of fruits of Chinese Quince (Chaenomeles speciosa) and peach (Prunus persica) showed circular, water-soaked and brown spots in July 2022 in Kunming, Yunnan, China. The center of these spots was covered by a large number of earthy brown and oblate sporogeneous mycelium containing conidiophore and conidia, which were one-celled, limoniform, hyaline (13.73 to 22.77 x 8.17 to 12.84 µm, n=50). By September 2022, almost 100% of fruits showed symptoms. Later, most of them fell or a few stiff, black and mummified fruits were left on the trees. Fungal isolates were isolated by single-spore technique on Potato Dextrose agar (PDA) from the diseased fruits, and incubated at room temperature (20-28 °C) in darkness for 14 days. The colony was gray, smooth at margins, 7.6-8.0 cm in diameter. To fullfill Koch's postulates, mycelial plugs of one representative isolate YHD611 from Chinese Quince and another YHD610 from peach were used to inoculate three wounded and three non-wounded surface-disinfected fruits of both hosts at room temperature (19-27 °C), respectively. Three wounded and three non-wounded fruits inoculated with sterile PDA plugs served as the control. The wounded peaches appeared water-soaked and had brown lesions after three days of inoculation, then completely decayed after nine days, while non-wounded fruits showed symptoms after five days. The wounded fruits of Chinese Quince developed similar symptoms after eight days of inoculation, and completely decayed after 13 days, while non-wounded fruits showed obvious symptoms after 15 days. In a subsequent study, isolate YHD611 was inoculated to peach while isolate YHD610 was inoculated to Chinese Quince to understand host specificity of the isolates. The results showed that when peaches were infected with YHD611, symptoms were observed on wounded fruits after three days while on non-wounded fruits after five days. When Chinese Quince was infected with YHD610, symptoms were observed on wounded fruits after 14 days while on non-wounded fruits after 21 days. Fungal isolates from symptomatic fruits were identical to the original isolates. There were no symptoms on the control fruits of both hosts. Molecular identification was confirmed based on the sequences of internal transcribed spacer (ITS, primers ITS1 and ITS4) and β-tubulin (TUB2, primers Bt2a and Bt2b) genes (Niu et al. 2016). BLASTn analysis of the ITS (OQ15519and OQ155196) and TUB2 (OQ185202 and OQ185201) of YHD611 and YHD610 revealed a 100% sequence identity, respectively, to Monilia yunnanensis AH7-2 (KT735924.1 for ITS, KT736008.1 for TUB2). In the phylogenetic analyses based on ITS and TUB2 sequence data, the isolates YHD611 and YHD610 belonged to the M. yunnanensis clade. Based on morphological and molecular identification, both isolates were identified as M. yunnanensis, which was reported as the pathogen causing brown rot of plum, peach, apple and pear in Yunnan, China (Hu et al. 2011; Yin et al. 2015). To our knowledge, this is the first report of M. yunnanensis causing brown rot on the fruits of Chinese Quince in Yunnan, China. This study also reports that M. yunnanensis from Chinese Quince can infect peach, and the pathogen from peach can infect Chinese Quince. These findings suggest that M. yunnanensis can transfer from one host to another and causing serious economic losses in multiple fruit crops in Yunnan, China.

Preservation, diagenetic transformation and paleoecological significance of fungal saccharides from lignites and fossil wood

Fungi are a common component of terrestrial ecosystems, although their preservation as molecular fossils is relatively rare. Saccharides, such as arabitol, mannitol and trehalose, are important in biochemical processes, and are the dominant compounds in fungi, lichens and yeasts. Here we present gas chromatography–mass spectrometry (GC–MS) analysis of fungal saccharides in brown coal extracts and compare their distribution to extant white, brown, and soft-rot fungi. Distribution differs depending on the type of decay in modern and fossil wood-decomposing fungi. An arabitol/(arabitol + trehalose) ratio higher than 0.6 for extant and fossil fungi is diagnostic for soft and brown rot, while values below 0.4 are characteristic of white rot fungi. Values between 0.4 and 0.6 suggest a mixed origin of fungal saccharides. The observed differences may shed light on the type of fungal activity, in particular relatated to climatic conditions of the Cenozoic.We conclude that saccharides can be good indicators, sensitive to climate change, of wood degradation by fungi in thermally immature organic matter. As a case study, early Cenozoic lignites were formed on low latitudes in the tropics during or near the Paleocene/Eocene thermal maximum, as extant soft rot fungi can withstand extremes of temperature and humidity. The presence of these fungi is confirmed by the dominance of arabitol over trehalose in most of the Lower Cenozoic lignites and arabitol/(arabitol + trehalose) ratio values higher than 0.6. In contrast, Miocene detritic coals, which were formed in temperate to subtropical climates, contain saccharides derived from a mixture of different fungi with white rot predominance.

Antifungal Potential and Chemical Composition of Chamaecyparis formosensis and Chamaecyparis obtusa var. formosana Essential Oils in Liquid and Vapor Phases Against 4 Fungal Species

Background Only a few studies have investigated the bioactivity of the essential oils (EOs) of Chamaecyparis formosensis and C. obtusa var. formosana, which are endemic to Taiwan. The wood and leaf EOs extracted from C. obtusa var. formosana had anti-inflammatory and antitermitic effects, respectively, and the twig EO extracted from C. formosensis inhibited the growth of Fusarium oxysporum, Pestalotiopsis funerea, and Ganoderma australe. Methods A poisoned food technique was used to evaluate the antifungal effects of wood and leaf EOs extracted from C. formosensis and C. obtusa var. formosana on brown rot fungus, white rot fungus, and mold. A phytatray chamber assay was conducted to investigate the antifungal activities of the EOs in the vapor phase. Gas chromatography-mass spectrometry was used to examine the chemical constituents of both EOs. Results The leaf EO extracted from C. formosensis primarily contained monoterpene hydrocarbons, whereas the leaf EO extracted from C. obtusa var. formosana primarily contained monoterpene and sesquiterpene hydrocarbons. The wood EOs extracted from C. formosensis and C. obtusa var. formosana primarily contained sesquiterpene hydrocarbons and oxygenated sesquiterpenes. These results indicated that the wood EOs had higher antifungal activity in the liquid phase, whereas the leaf EOs had higher antifungal activity in the vapor phase. Conclusion Wood EOs extracted from C. formosensis and C. obtusa var. formosana have strong antifungal effects against Gloeophyllum trabeum, Lenzites betulina, Trametes versicolor, and Trichoderma sp. Compared with wood EOs, leaf EOs have a greater inhibitory effect on mycelial growth in the vapor phase. In both the liquid and vapor phases, the EOs extracted from C. formosensis and C. obtusa var. formosana serve as effective antifungal agents against pathogenic fungi. Further research is required to elucidate the mechanisms underlying the antifungal activities of EOs in different phases and explore their potential applications.