Broomrape Races Research Articles

Гербицидоустойчивый сорт подсолнечника кондитерского типа Аладдин

The variety Aladdin is the new confectionary sunflower variety. It is developed in the laboratory of OP-sunflower breeding jointly with the laboratory of genetic at the V.S. Pustovoit AllRussian Research Institute of Oil Crops in 2014– 2020. It is developed by crossing plants of a line VK 1-imi (Imr Imr) used as a maternal form and a pollen mixture of an early maturing confectionary sunflower variety, with the further treatment by the herbicide Euro-Lightning. Later, there were conducted a multiple individual selection and estimation in progenies of families that were the best by their morphometric and economically valuable traits, tolerance to broomrape races E, F, and G and to downy mildew. The variety belongs to the middle maturity group, confectionary type. The major features of the cultivar Aladdin are its resistance to imi-herbicides, high productivity, tolerance to the main races of broomrape and downy mildew, increased level of autofertility. The variety is suitable for cultivation by Clearfield (BASF) technology. It is uniformed by plants height, flowering and maturing. A period emergence – physiological maturity is equal to 97–99 days. Weight of 1000 seeds at plant population of 25–30 thousand plants/ha exceeds 130 g.

Гибрид подсолнечника Статус

The widespread saturation of crop rotations with sunflower crops contributed to the creation of favorable conditions for the emergence and wide distribution of new more aggressive races of broomrape, downy mildew and other pathogens of this crop. Sunflower varieties and hybrids resistant to previously common races have become severely affected. One of the ways to solve this problem is to develop sunflower hybrids resistant to new virulent races. The breeders had to combine high productivity and genes of resistance to new more aggressive races of broomrape and downy mildew in one genotype. The first stage of the work was development of a new breeding germplasm and selection of parental lines with breeding valuable traits from it. Based on these lines, there were developed hybrid combinations resistant to new highly virulent races of broomrape and downy mildew. The hybrid Status was developed by hybridization of a maternal line ED 47 and a paternal line ED 193. The maternal line ED 47 is single-headed, low height, resistant to the broomrape races: E, F, G, has a good combining ability. The paternal line ED 193 is branched, also resistant to broomrape (races E, F, G) and three races of downy mildew (330, 710, and 730). The hybrid Status belongs to the middle-early maturity group, duration of the growing season is 95 days. The average plant height over the years of testing was 140 cm; the plants are uniformed in height and the duration of the development phases. Seed yield of the hybrid Status exceeded the standard one (a hybrid Gorstar) by 0.25 t/ha, and oil yield – by 0.14 t/ha. The oil content of the seeds was 48.0%, the huskiness – 28.7%, the 1000 seeds weight – 60.6 g. The new hybrid is resistant to seven races of broomrape (A, B, C, D, E, F, and G) and three races of downy mildew (330, 710, and 730). A distinctive feature of it is resistance of both parent lines to broomrape. The originator of a simple interline sunflower hybrid Status is V.S. Pustovoit All-Russian Research Institute of Oil Crops.

Genetic and physiological characterization of sunflower resistance provided by the wild-derived OrDeb2 gene against highly virulent races of Orobanche cumana Wallr

Key messageOrDeb2 confers post-attachment resistance to Orobanche cumana and is located in a 1.38 Mbp genomic interval containing a cluster of receptor-like kinase and receptor-like protein genes with nine high-confidence candidates.Sunflower broomrape is a holoparasitic angiosperm that parasitizes on sunflower roots, severely constraining crop yield. Breeding for resistance is the most effective method of control. OrDeb2 is a dominant resistance gene introgressed into cultivated sunflower from a wild-related species that confers resistance to highly virulent broomrape races. The objectives of this study were as follows: (i) locate OrDeb2 into the sunflower genome and determine putative candidate genes and (ii) characterize its underlying resistance mechanism. A segregating population from a cross between the sunflower resistant line DEB2, carrying OrDeb2, and a susceptible line was phenotyped for broomrape resistance in four experiments, including different environments and two broomrape races (FGV and GTK). This population was also densely genotyped with microsatellite and SNP markers, which allowed locating OrDeb2 within a 0.9 cM interval in the upper half of Chromosome 4. This interval corresponded to a 1.38 Mbp genomic region of the sunflower reference genome that contained a cluster of genes encoding LRR (leucine-rich repeat) receptor-like proteins lacking a cytoplasmic kinase domain and receptor-like kinases with one or two kinase domains and lacking an extracellular LRR region, which were valuable candidates for OrDeb2. Rhizotron and histological studies showed that OrDeb2 determines a post-attachment resistance response that blocks O. cumana development mainly at the cortex before the establishment of host-parasite vascular connections. This study will contribute to understand the interaction between crops and parasitic weeds, to establish durable breeding strategies based on genetic resistance and provide useful tools for marker-assisted selection and OrDeb2 map-based cloning.

Efficiency of selection–biotechnological system of selection for creation of breeding source material of sunflower resistant to herbicides and broomrape

Abstract The sunflower is a strategically important oil crop. Every year the area under this crop grows, and the rapid returning of sunflowers back to the fields provokes the formation of new more aggressive races of broomrape (Orobanche cumana Wallr.). Broomrape is a parasite that interferes with the normal development of sunflower and can lead to significant crop losses. For creating a sunflower hybrid (F1) it is needed to cross the parental components, which have a complex of important traits, among which there is a resistance to the herbicides and a broomrape. Considering that the creation of each of the components of the hybrid involves many years of painstaking work in the breeding process, modern approaches and methods are used to accelerate the creation of a new source material. Thus, using the technology of cultivating immature embryos in vitro culture, it is possible to reduce the time to create lines resistant to herbicides, for example. And during selection for resistance to pathogenic organisms, testing is most often used against an artificial infectious background, both in the field and in laboratory conditions, in order to differentiate the material on this basis. The aim of this work was to establish the effectiveness system when creating an initial breeding material resistant to herbicides and broomrape. As a result of testing the lines on an artificial infectious background, was identified plants which have high resistance to the G-race broomrape and were isolated from hybrid combinations resistant to tribenuron-methyl and imidazolinones. Thus, among the analyzed plants which are resistant to tribenuron‐methyl, four lines were isolated, which are highly resistant to the G-race broomrape from a hybrid combinations BH0118/SURES–2 (101/1, 101/4, 101/6, 101/7), and BH0318/SURES–2 (101/21, 101/24, 101/28, 101/30), and five lines (101/11, 101/12, 101/16, 101/17, 101/18) from a hybrid combination BH0218/SURES–2. Among imidazolinone-resistant sunflower lines – line 3 was isolated as highly resistant to the G-race broomrape.

Breeding of sunflower lines resistant to race G of Broomrape

Relevance. Over the past decades, new and extremely virulent races have appeared in the southern regions of the Russian Federation, which overcame the resistance of domestic and foreign sunflower range of varieties. The most effective, long-term, and biologically safe way to protect sunflower from broomrape is to develop varieties and hybrids resistant to new races.Methods. We identified broomrape seeds of race G using internationally accepted differentiator lines: Record 1-3 (C), S-1358 (D), P-1380 (E), LC1093 and P96 (F). We used sunflower lines and hybrids of the breeding of V.S. Pustovoit All-Russian Research Institute of Oil Crops as a parent material. We carried out the evaluation of broomrape resistance by the method of A.Ya. Panchenko. To develop an infectious background, we introduced seeds of broomrape race G into boxes with a soil and sand mixture at the rate of 200 mg per 1 kg of the mixture, distributing them evenly. We grew sunflower plants at a temperature of 25-27 °С and a 16-hour photoperiod. In 25-30 days after the emergence if seedlings, we dug up plants and counted the broomrape specimens on their roots by a scale.Results. We developed sunflower lines resistant to races of broomrape from A to G. All of them are of branched form, with different lengths of the period from germination to flowering, they differ in height and oil content. The line L 82 is distinguished by a high content of oleic acid in oil — 91%. The lines have a high combinability. We obtained experimental hybrid combinations using these lines.

Modern trends in breeding and genetic improvement of sunflower varieties and hybrids at VNIIMK.

Modern sunflower breeding is significantly diversified due to the different needs of agricultural production. The breeding of sunflower varieties and hybrids is carried out at V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK) in all areas in demand on the market and is based on fundamental biological research. In the field of breeding for faster maturing, the following commercial cultivars were obtained: very early maturing, cv. Skormas and the three-way hybrid Achilles, early maturing cvs. Varyag and Uspekh, medium maturing cvs. Amelie, Aris and Aurus. Within the framework of breeding for immunity, eight hybrids and one variety have been produced. So at the Don experimental station (Rostov region), productive hybrids were bred, resistant to the virulent broomrape of the G race due to the presence of the Or7 gene: ‘Gorstar’, ‘Gorfild’, ‘Grant’, ‘Status’, ‘Fogor’ and the three-way hybrid Nika. On the central experimental base, the following were obtained: the mid-early hybrid Typhoon and the earlymaturing variety Platonych with resistance to common races of downy mildew and a high oil content of achenes (up to 53 %) as well as the mid-early hybrid Tayzar, which is simultaneously resistant to virulent races of broomrape and to the causative agent of downy mildew. The early maturing large-fruited sunflower variety Belochka was included in the “Russian State Register of Selection Achievements…”, and the large-fruited varieties Karavan, Konditer and Kalibr are currently undergoing state tests. The breeding use of germplasm with genes for herbicide resistance was accompanied by their extensive genetic study. A practical recommendation for all three alleles of the ALS gene (Imr, CLHA-Plus, Sur) was the need to create homozygous hybrids for their reliable use in appropriate production systems. For Clearfield technology, the hybrids Imidzh, Arimi and Immi have been developed; for Clearfield Plus, the hybrid Klip; and for Express Sun (or SUMO), the hybrid Surus. Klip and Surus are mid-oleic. All newly developed fertile ornamental sunflower varieties – Aurelia, Fizalia, Zhemchuzhny, Rumyanets, Agat and Mazhor – were transferred for practical use to a sterile CMS RIG basis. Thus, new achievements have been attained across the entire spectrum of modern trends in sunflower breeding.

Анализ сцепления гена устойчивости к расе G заразихи с микросателлитными локусами у линии-донора подсолнечника селекции ВНИИМК RGP1

Broomrape (Orobanche cumana Wallr.) is one of the main biotic factors limiting high sunflower yield formation. The most effective and environmentally safe method of protection is cultivation of resistant varieties and hybrids of sunflower. Development of resistant sunflower genotypes includes search and usage of sources of resistance in breeding process as well as accurate and productive procedures of material assessment. The purpose of the research is to analyze a linkage of a gene Or7 with microsatellite loci of the line-donor of resistance to broomrape race G from the VNIIMK’s collection. The objects of the research are the line RGP1 – a donor of resistance to broomrape race G and a susceptible to this race line VR 678 from the VNIIMK’s collection. Sunflower plants were crossed in field to produce F1. Also we conducted self-pollination of F1 plants to obtain F2 progeny. Plants were tested in a greenhouse in soil infected with seeds of broomrape race G using a method of early diagnostic. Sunflower DNA was extracted from the top leaves of the young sprouts of the vegetative plants. For PCR-analysis we used three SSR-primers demonstrated polymorphism in parental lines: ORS 683, ORS 1040, and ORS 1112. We tested joint inheritance of the gene Or7 and these loci, and inheritance between SSR-loci. An independent inheritance of the gene Or7 with DNA-loci ORS 683, ORS 1040, and ORS 1112, as well as SSR-loci between ORS 1040 and ORS 1112, ORS 1040 and ORS 683 was showed. Loci ORS683 – ORS 1112 are linked with a frequency of recombination of 0.27 ± 0.41 (27 cM). As a result of our research location of the gene Or7 in the nearest area to microsatellite loci ORS 683, ORS 1040, and ORS 1112 was excluded. Basing on studied literary sources and a representative sunflower genome HanXRQr2.0-SUNRISE we made a partial physical map LG3 for determination of an area for the further search of a localization of the Or7 and DNAmarkers co-segregating with this gene.

VARIABILITY OF SOME BROOMRAPE POPULATIONS FROM CHINA. MORPHOMETRY AND RACE IDENTIFICATION (I)

The article is a synthesis of some studies on the identification of broomrape races originating from China and the determination of the level of intra- and interpopulation variability based on the morphometric parameters of the seeds. The racial status of the analyzed populations was established, also two races of broomrape were identified: race G (Ch1 from Inner Mongolia and Ch3 from HeBei) and race H (Ch2 from Inner Mongolia) using internationally recognized differential Or homosygous lines set. Comparative analyses on the morphometric characteristics of broomrape revealed significant differences in seed Length (Ch1 and Ch3 populations, race G) and Length/width ratio (Ch3 and Ch4), which is an indication of the moderate level of interpopulation variability. In the case of assess of the intrapopulation variability level, the relatively homogeneous (Ch2, Ch3 and Ch4) and relatively heterogeneous (Ch1) populations were identified. According to the coefficient of variation, the population Ch2 identified as H broomrape race has distinguished as the most homogeneous for the investigated biometric indices of the seeds. The moderate positive correlations (between Length and width seed, between seed length and L/w ratio), the strong positive correlations (between Length and L/w ratio) and also the strong negative correlations (between width and L/w ratio) were established. The results obtained through Pearson coefficient revealed the discrimination of populations by region.

The search for the location of the sunflower resistance gene to race G of broomrape on the linkage map in the lines of breeding of VNIIMK

The parasitic plant Orobanche cumana Wallr. is one of the main biotic limiting factors for obtaining high yields of sunflower. Since plant resistance to different broomrape races in different genotypes can be controlled by different genes, to ensure the evaluation of breeding material using DNA markers (MAS), it is necessary to localize the resistance gene for each of the sources. The aim of the research is to find the location of the Or7 gene, which controls the resistance to one of the most virulent broomrape races, on the sunflower linkage map and to determine the DNA markers cosegregating with it. For research we used the lines of breeding of V.S. Pustovoit of All-Russian Research Institute of Oil Crops (VNIIMK) resistant and susceptible to race G of broomrape. We used SSR-primers ORS 683, ORS 1040, ORS 1112, and ORS 202 for the PCR analysis. We excluded the location of the gene in the upper part of LG3 in the immediate proximity to the microsatellite loci ORS 683, ORS 1040, ORS 1112. Based on the studied literary sources and the reference genome of the sunflower HanXRQr2.0-SUNRISE, we compiled a partial physical map of LG3 and determined the area for further search for the localization of the Or7 gene and DNA markers cosegregating with it.

Dynamics of change in broomrape populations (Orobanche cumana Wallr.) in Romania and Russia (Black Sea area)

Abstract The emergence of new broomrape populations (races) has been observed in the past 20 years in several countries (Romania, Moldova, Ukraine, Russia, Turkey, Bulgaria, Spain, Serbia, and China) where sunflower is frequently grown in the same sites without applying traditional crop rotations. Differential lines for sunflower broomrape races A to F have been secured. The new broomrape races have been identified by researchers as races G and H. The question of whether the same broomrape mutations can occur in one year and affect the same countries remains unsolved. Several results of new broоmrape population emergences in some of the affected will be presented in the paper. A total of 390 genotypes were studied at four Romanian localities (Cuza Voda, Crucea-Stupina, Braila-Valea Canepii, and Tulcea-Agighiol) in 2014. At all four localities, a certain degree of sunflower broomrape infestation was observed in control hybrids and lines (Performer, LC-1093, LG-5661, and PR64LE20), which indicated the emergence of new populations higher than race H. The 390 studied genotypes had different reactions in all four localities. In 2015, 10 hybrids and controls were studied at five Romanian localities (Ciresu-Braila, Iazu-Ialomita, Stupina-Constanta, Topolog-Tulcea, and Viziru-Braila) and, according to the results, only hybrid Hy-7 was resistant in all localities. The results obtained from the three studied localities showed the emergence of new sunflower broomrape populations not controlled by gene for race H. Self-fertilization of hybrid Hy-7 produced the F2 generation in 2016. In 2017, broomrape resistance was studied at the infested (contaminated) plot at the All-Russian Research Institute of Oil Crops by the name of Pustovoit V.S. – VNIIMK in Rostov on Don. The plot was found to be infested by new broomrape populations originating from Russia, Ukraine, Romania, Turkey, and Spain. The obtained results showed an infestation degree in 17.1% plants of hybrid Hy-7, 35% in the F2 generation of Hy-7, control hybrids PR64LE25, LG-5580 and Donskoy-22 showed 19.4, 23, and 100% broomrape infestation, respectively. In conclusion, the plot contained broomrape populations which cannot be controlled by race H gene. According to the obtained results, a permanent change in variability of broomrape populations can be confirmed practically year after year. At present, new broomrape populations found at several localities are locally dispersed. Geneticists and breeders have to make joint efforts in further detailed studies of broomrape variability.

Заразихоустойчивый гибрид подсолнечника Тайзар

A simple sunflower hybrid Taizar was developed at V.S. Pustovoit All-Russian Research Institute of Oil Crops as part of a breeding and genetic program for developing highly productive hybrids resistant to the new virulent races of broomrape by using the method of backcrossing in combination with an evaluation of resistance in each generation for the target gene Or7. The hybrid Taizar is of mid-early group of ripeness, it has a high yield, it is resistant to Orobanche cumana Wallr. (races A-G) and downy mildew (races 330, 710, 730, 334, 734). The line-restorer of pollen fertility VK 305 is the paternal form of the hybrid Taizar. The line was developed as a result of the introduction of the Or7 gene from the RG line by two backcrosses into the line VK 303, subsequent inbreeding and selection of broomrape-resistant genotypes. The line VK 305 has narrow widely spaced ray flowers strongly curved towards the back of the head, which can serve as a morphological marker of genetic purity.

История селекции подсолнечника в России на устойчивость к заразихе (обзор)

The history of sunflower breeding in Russia is permanently connected with breeding for resistance to broomrape (Orobanche cumana Wallr.) – a parasitic plant. The most important task for the scientists was to develop vrieties resistant to broomrape. V.S. Pustovoit, L.A. Zhdanov, E.M. Plachek and other breeders have developed broomrape tolerant sunflower varieties. Their introduction into agricultural production played a huge role in keeping and increasing of sunflower productivity. As evolution of host-plant and parasite develops inseparably, during natural selection of resistant varieties and hybrids there are appeared the new more aggressive races of broomrape which are able to live and multiply. The development of the new aggressive races must be controlled permanently. It can be reached by development of the new resistant sunflower varieties and hybrids.

Трёхлинейный гибрид подсолнечника Ника

A three-line sunflower hybrid Nika was developed by hybridization of a simple sterile hybrid Donskoy 59 and a line-restorer of pollen fertility ED 155 at the Don experimental station. It is characterized by high productivity, over the years of testing, it exceeded the control on seed yield by 0.25 t per ha, on oil collection per hectare by 0.16 t per ha. According to the length of the vegetative period (98–104 days), it can be attributed to the middle-early group. Plant height is up to 170–180 cm. The leaves are large, the bubbling is very weak, and serration is large. Heads of medium size when maturing turned down with a straight stem. Achenes are broadly ovate, black with grey stripes along the edges and between the edges. The main distinguishing feature of the hybrid is its high productivity and resistance to the new aggressive races of broomrape (E, F, G) and downy mildew (race 330). The hybrid Nika has been included into the State register of protected breeding achievements of the Russian Federation since 2019 and allowed to be cultivated in the Central Black Soil (5), Northern Caucasus (6), Middle Volga (7), Low Volga (8), Ural (9), and Western Siberian (10) regions of Russia.

Поиск и анализ нуклеотидных последовательностей в геноме подсолнечника, ассоциированных с генами устойчивости к расам заразихи Orobanche cumana Wallr.

Studying of sunflower lines resistant to broomrape race G, bred in V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK), Krasnodar showed the genetic control of resistance is monogenic with incomplete dominance. The purpose of the research was to seek and analyze molecular markers for the genes of resistance to broomrape race G using PCRmethods. In our research we used six developed in VNIIMK lines-donors resistant to broomrape race G: RGP1, RGP2, RGB, RGL1, RGL2, RGA, and susceptible lines VK 101, VK 678, VK 680. We extracted DNA from the leaves of young sunflower plants using STAB-buffer. For PCR-analysis we used 17 pairs of primers of two types: SCAR (sequence characterized amplified region) and SSR (simple sequence repeat). Conditions of amplification are as recommended by authors of markers with experimental selection of a temperature for primers hybridization. Within the research we balanced involved into work SSR- and 12 SCAR-loci using BLAST and a set of HanXRQr1.0. Due to these data we composed a physical map of loci location. After assessment of six resistant and three susceptible parental lines, seven markers demonstrated polymorphism by a length of locus DNA and two ones – by presence/absence of amplified fragment. For markers ORS 683 and ORS 1112 we observed a relation between loci polymorphism and susceptibility of parental lines to broomrape. This was certified with a presence of an allele of 364 n.p. length in ORS 683 and an absence of an allele of 375 n.p. length in ORS 1112. These markers were noted as primary ones for hybridological analysis in F1 и F2 with selected pairs for crossing. Additional markers will be selected individually using obtained data on loci polymorphism. Thus, as a result of the research we created a physical map for the further markers selection, found hybrid combinations and markers from the studied ones for hybridological analysis.

The source material for the selection of decorative sunflower

Seven lines of analogs with different color of leaves were created in sunflower by the backcross method of the recipient line of YuV-28B and gene donors controlling different variations in leaf color. A study of these lines at the Research Institute of Agriculture of the South-East region in 2017–2019, conducted as a competitive variety test, showed that most seed lines did not significantly differ in seed yield from the recipient line YuV-28B except for the L-4 line with olive color of the sheet, in which this indicator was lower than that of the recipient line. They were assessed analogous lines according to the phenological, morphological, crop-forming characteristics, as well as according to the quality of seeds and oil. All analogue lines had 100% shell of seeds and resistance to local races of broomrape and downy mildew. Most of the studied indicators of these lines correspond to the parameters of the source material, so they can be used in the selection of decorative sunflower.

Development of the initial sunflower breeding material resistant to new high virulent races of broomrape and false powdery mildew

The widespread violation of scientifically based crop rotation and their saturation with sunflower caused an accelerated emergence of more aggressive races of broomrape and powdery mildew. Currently, the researchers from different countries have identified 8 races of broomrape A, B, C, D, E, F, G, H. The sunflower varieties and hybrids previously developed at the station and introduced into the State List of Breeding Achievements of the Russian Federation that are resistant to the broomrape races A, B, C, D, E were greatly affected. Monitoring of the races of broomrape and false powdery mildew, conducted by the ARRIOC researchers, showed the presence of more aggressive broomrape races (F, G, H) and false powdery mildew (330, 710, 730) in the fields of the Rostov Region. The current situation necessitated the development of sunflower hybrids resistant to the new aggressive races of these pathogens. To identify such hybrids, the corresponding initial breeding material has been required. It was important to combine resistance to broomrape and false powdery mildew with other breeding-valuable traits in one genotype. The current paper presents the results of the sunflower initial breeding material on resistance to broomrape and false powdery mildew. The work was carried out in the fields and in the laboratories of DOS, an affiliate of the FSBSI FSC ARRIOC. The breeding material developed at the station was used as the initial material. Using hybridization methods, multiple self-pollination, and reliable estimation of pathogen resistance at all stages of the breeding process, there was selected a promising linear material (I-4/4640, I-4/4764, I-5/2450, I-5/2465 and etc.), which was included into the search for high heterosis hybrid combinations. The hybrid combinations developed with its participation (MLG 45/4640, MLG 121/4764, MLG 65/2444, etc.), when estimated on the fields infected by sunflower broomrape, showed a higher resistance to highly virulent races of the pathogen in comparison with the best foreign hybrids.

Sunflower Production in Blacksea Region: The Situation and Problems

Sunflower is one of the most important oil crops in the world. In addition to be the most preferable vegetable oil in the Eastern Europe, Balkans and Blacksea region, sunflower is the main crops in the rotation system in the agricultural production as well as the most planted oil crops in these areas. Both world sunflower planted areas (21.000 ha in 2007 to 26.000 ha in 2019) and also seed production (26.000 MT in 2007 to 51.000 MT in 2019) have raised in last ten years but Blacksea region contributed mainly for these increases. In the region, while sunflower production has tripled from 11800 MT (2007) to 35.000 MT (2019), sunflower planted areas has raised 60% more from 2007 to 2019 (10.000 to 16.000 ha) in last 12 years. The rate of region in sunflower production has increased from 45 to 68%, acreages from 49 to 64% in this period. Furthermore, the region plays key role and also dominated sunflower world trade as well as other oilseeds such as canola and soybean. As a spring crop, sunflower influence eventually from climatic conditions such as temperature, soil humidity, etc. in some critical stages which are determining seed and oil yield especially bud formation and the grain filling period. Biotic and abiotic stresses coincide in these two stages lead extremely reduce seed yield regularly in sunflower production areas in due to unregularly rainfalls and higher temperatures in spring and early summer seasons in Blacksea region which sunflower grows mostly in dryland. Therefore, it need to well understand these reducing factors and sunflower responses and mechanisms then it need to find accurate management solutions to cope with these factors efficiently such as early planting time and increasing plant density for obtaining better yields. Besides, biotic stresses mostly diseases such as downy mildew, Phomopsis, Sclerotinia, Verticilium wilt, Macrophomina and also broomrape parasite, weeds are the main factors reducing sunflower yield in the region. While there is only genetic resistance possible for disease control mentioned above ones, effective control of broomrape and weeds are possible Clearfield system with post emergence Imidazolinone (IMI) herbicide plus resistant varieties as well as sulfonyl Urea (SU) herbicide and resistant hybrids. Furthermore, combining these herbicide resistances together with new races of broomrape races and downy mildew and other diseases will lead higher yield results in sunflower production in the region in addition to other agronomical solutions.

Genetic and Genomic Tools in Sunflower Breeding for Broomrape Resistance.

Broomrape is a root parasitic plant causing yield losses in sunflower production. Since sunflower is an important oil crop, the development of broomrape-resistant hybrids is the prime breeding objective. Using conventional plant breeding methods, breeders have identified resistant genes and developed a number of hybrids resistant to broomrape, adapted to different growing regions worldwide. However, the spread of broomrape into new countries and the development of new and more virulent races have been noted intensively. Recent advances in sunflower genomics provide additional tools for plant breeders to improve resistance and find durable solutions for broomrape spread and virulence. This review describes the structure and distribution of new, virulent physiological broomrape races, sources of resistance for introduction into susceptible cultivated sunflower, qualitative and quantitative resistance genes along with gene pyramiding and marker assisted selection (MAS) strategies applied in the process of increasing sunflower resistance. In addition, it presents an overview of underutilized biotechnological tools, such as phenotyping, -omics, and genome editing techniques, which need to be introduced in the study of sunflower resistance to broomrape in order to achieve durable resistance.

The environmentally safe method of control of broomrape (Orobanche cumana Wallr.) parasitizing on sunflower

Orobanche cumana Wallr., an obligate parasite of sunflower, is widely spread in most countries cultivating this crop. It is one of the main factors limiting the cultivation of sunflower. The aim of our research was to determine the influence of application of oil crops (Brassicaceae) green manures on the sunflower infection with broomrape under greenhouse conditions. We used the green mass of white, black, common mustard and winter rapeseed as a green manure. We added the crushed green mass of these crops (62.5-90.0 g) to vessels with a soil-sand mixture mixed with broomrape seeds. After 15 days, we sowed the seeds of sunflower variety susceptible to all races of broomrape. We did not add the green manures in the control. The green manure of white mustard (variety Ruslana) showed the highest percentage of decrease in the infection degree – 44.7 %. The common mustard (Yunona) and black mustard (Niagara) reduced the infection by 25.9 and 27.0 %, respectively, the rapeseed (Tavrion) – by 24 %. We recommend using the green manures of these crops to reduce infection of sunflower crops with broomrape and as an environmentally safe method of clearing the field from its seeds.

НАСЛЕДОВАНИЕ УСТОЙЧИВОСТИ ЛИНИИ ПОДСОЛНЕЧНИКА RGM К РАСЕ G ЗАРАЗИХИ В F

НАСЛЕДОВАНИЕ УСТОЙЧИВОСТИ ЛИНИИ ПОДСОЛНЕЧНИКА RGM К РАСЕ G ЗАРАЗИХИ В F