Broiler Breeder Hens Research Articles

The Diverse Roles of 17β-Estradiol in Non-Gonadal Tissues and Its Consequential Impact on Reproduction in Laying and Broiler Breeder Hens.

Estradiol-17β (E2) has long been studied as the primary estrogen involved in sexual maturation of hens. Due to the oviparous nature of avian species, ovarian production of E2 has been indicated as the key steroid responsible for activating the formation of the eggshell and internal egg components in hens. This involves the integration and coordination between ovarian follicular development, liver metabolism and bone physiology to produce the follicle, yolk and albumen, and shell, respectively. However, the ability of E2 to be synthesized by non-gonadal tissues such as the skin, heart, muscle, liver, brain, adipose tissue, pancreas, and adrenal glands demonstrates the capability of this hormone to influence a variety of physiological processes. Thus, in this review, we intend to re-establish the role of E2 within these tissues and identify direct and indirect integration between the control of reproduction, metabolism, and bone physiology. Specifically, the sources of E2 and its activity in these tissues via the estrogen receptors (ERα, ERβ, GPR30) is described. This is followed by an update on the role of E2 during sexual differentiation of the embryo and maturation of the hen. We then also consider the implications of the recent discovery of additional E2 elevations during an extended laying cycle. Next, the specific roles of E2 in yolk formation and skeletal development are outlined. Finally, the consequences of altered E2 production in mature hens and the associated disorders are discussed. While these areas of study have been previously independently considered, this comprehensive review intends to highlight the critical roles played by E2 to alter and coordinate physiological processes in preparation for the laying cycle.

Supplemental effects of amino acid-complexed trace minerals on broiler breeder hen performance

A broiler breeder hen study was conducted to evaluate the replacement of trace element sources from inorganic minerals (IM) by amino acid complexed minerals (AACM) sources. Zn, Mn, Cu, and Fe sulfate, Na selenite and K iodate were the inorganic sources whereas the organic were supplemented from amino acid complexed sources. A total of 84 Cobb 500 38-week-old broiler breeder hens were individually caged and fed diets as follows: T1- Control IM sources with Zn, Mn, Cu, Fe, Se and I at 100, 100, 20, 60, 0.35 and 2 ppm, respectively; T2- same total mineral content as in T1 in a combination of IM and AACM sources (Zn: 60/40, Mn: 60/40, Cu: 13/7, Fe: 40/20, Se: 0.20/0.15, and I: 0/2 ppm); T3- only IM sources content from T2; T4- only AACM sources content from T2; T5- IM sources with Zn, Mn, Cu, Fe, Se and I at 120, 120, 25, 70, 0.40 and 3 ppm, respectively; T6- Same total mineral content as in T5 in a combination of IM and AACM sources (Zn: 60/60, Mn: 60/60, Cu: 13/2, Fe: 40/30, Se: 0.20/0.20, and I: 0/3 ppm); T7- only AACM mineral source content from T6. Evaluation was done from week 40–51. There were no significant effects of the treatments for egg production, incubation, egg quality and hatching chicks (P > 0.05) during this period. One exception occurred for eggshell thickness, which was increased when hens were fed partial and total replacement of AACM (P < 0.05). Contrasts between total replacement with AACM and IM supplemented diets showed an increase in settable eggs when breeders were fed AACM. Higher hatchability of fertile eggs was observed in partial replacements with AACM treatments when compared to IM (P < 0.05). Also, hatching chick had higher length and more chicks with leg score 1 in total replacement of AACM compared to the partial AACM replacement and IM groups (P < 0.05), and IM groups (P < 0.05), respectively. In conclusion, totally or partially replacement with IM by AACM led to improvements in eggshell and chick quality.

Ad libitum feeding alters mRNA abundance in the ovarian cortex of broiler breeder hens.

Ad libitumfeeding in broiler breeder (BB) hens causes reduced egg production, lower fertility, and improper eggshell deposition. Restricted feeding (RF) is the only effective intervention available to normalize ovarian function and improve reproductive efficiency. This study aimed to assess the transcriptional changes in ovarian cortex of BB hens with free access to feed compared to those on a RF diet. RNA was isolated from the ovarian cortex of Cobb 500 pullets raised to 10 and 16 weeks of age on either a full-feeding (FF) or RF diet. Microarray analysis identified 386 differentially expressed genes between the two feeding groups at 16 weeks of age. Gene ontology enrichment identified overrepresentation of Neuroactive ligand–receptor interaction pathways, Cell adhesion molecules, Steroid hormone biosynthesis, and various KEGG pathways. From these groups, 46 genes were selected for follow-up validation by quantitative PCR. The findings show that 33 of the 46 genes had significantly different abundance by age and/or feeding level. Most of these genes were repressed in RF hens and belonged to the steroid biosynthesis and neuropeptide signaling groups. The VIPR2 receptor was higher in the FF group leading us to hypothesize that vasoactive intestinal peptide (VIP) is an important regulator of small cortical follicles. Culture of hen cortical follicles with VIP increased Star, an indication of increased steroidogenic activity, although did not elevate Cyp11a1. These results offer insights and suggest the possible mechanisms and pathways responsible for the increases in cortical follicle growth associated with excess feed intake in BB hens.Lay summaryGiving breeder hens unrestricted access to feed can lead to problems with their ovaries, including excessive growth of the ovary and reduced fertility. Giving a limited amount of feed is the only effective way to reduce this growth of the ovaries and improve fertility. This study aimed to assess the changes in the molecules that make proteins in the body in hens fed unrestricted and restricted diets. In the hens fed a limited amount of feed, there were more of one type of molecules, while there were more of another type in the ovaries of hens with unrestricted access to feed. These results show that how much a hen eats can alter the number of these molecules in the ovary and this could help us understand why their ovaries grow excessively and why their eggs are less fertile.

Phenotypic Correlations Between Egg Quality Traits Amid the Laying Phase of Broiler Breeder Hens

Summary In order to determine phenotypic correlations between the quality traits of eggs from 41-week-old broiler breeder hens (Cobb 500), a total of 105 eggs suitable for incubation were analyzed using descriptive statistics and Pearson’s correlation coefficient. The average values of egg weight, egg length, egg width, egg shape index, shell thickness, shell weight, and shell ratio were 66.90 g, 60.00 mm, 44.81 mm, 74.70%, 0.37 mm, 6.07 g, and 9.07%, respectively. Moreover, the average values of yolk weight, albumen weight, yolk ratio, albumen ratio, Haugh units, yolk index, and yolk-to-albumen ratio were 20.04 g, 40.80 g, 29.97%, 60.95%, 82.12, 46.17%, and 0.49, respectively. A significant positive correlation (p &lt; 0.01) was found between the egg weight and the egg length (0.75), egg width (0.80), shell (0.55), yolk (0.60), albumen weight (0.91), and albumen ratio (0.25). However, a significant negative correlation (p &lt; 0.01) was found between the egg weight and both the yolk ratio (-0.20) and yolk-to-albumen ratio (-0.23). The egg shape index proved not to be significantly correlated with any of the internal egg quality traits considered. The shell thickness was in a significant positive correlation (p &lt; 0.01) with the shell weight (0.83) and ratio (0.86), whereas a significant negative correlation (p &lt; 0.05) was found between the shell thickness and yolk ratio (-0.23). The Haugh units were in a significant positive correlation (p &lt; 0.05) with the albumen ratio (0.24) and yolk index (0.27), whereas a significant negative correlation (p&lt;0.05) was found between the Haugh units and both the yolk ratio (-0.22) and yolk-to-albumen ratio (-0.23). The results obtained confirm significant phenotypic correlations not only between egg weight and the egg quality traits considered, but also between a number of external and internal quality traits of broiler hatching eggs.

Combining Maternal and Post-Hatch Dietary 25-Hydroxycholecalciferol Supplementation on Broiler Chicken Growth Performance and Carcass Characteristics

Dietary inclusion of the vitamin D3 (D3) metabolite, 25-hydroxycholecalciferol (25OHD3), was demonstrated to improve broiler growth performance and breast meat yield. To assess the effect of combined maternal (MDIET) and post-hatch (PDIET) dietary 25OHD3 inclusion on broiler growth performance and carcass characteristics, a randomized complete block design experiment with a 2 × 2 factorial treatment structure was conducted. From 25 to 38 weeks of age, broiler breeder hens were provided with 1 of 2 MDIET formulated to contain: 5000 IU D3 (MCTL), or 2240 IU of D3 + 2760 IU of 25OHD3 per kg of feed (M25OHD3). Their chick offspring (n = 448; 224 per MDIET) hatched from eggs collected from 37 to 38 weeks of age were reared in 16 replicate pens with 7 birds per pen and fed 1 of 2 PDIET in 3 phases up to day 40 formulated to contain: 5000 IU of D3 per kg of feed (PCTL), or 2240 IU of D3 + 2760 IU of 25OHD3 per kg of feed (P25OHD3). No additive or synergistic effects of combining 25OHD3 inclusion in MDIET and PDIET were observed. Broilers from 25OHD3-fed hens (M25OHD3) were heavier on day 40 than those from hens fed only D3 (MCTL; 2.911 vs. 2.834 kg; p = 0.040). Tender weight (123 vs. 117 g) and yield (5.63 vs. 5.44%) were greater in the M25OHD3 broilers than the MCTL broilers (p = 0.006). Broilers fed 25OHD3 (P25OHD3) tended to have heavier breasts (637 vs. 615 g; p = 0.050), bone-in wings (215 vs. 210 g; p = 0.070), and boneless thighs (279 vs. 270 g; p = 0.078) compared with those fed only D3 (PCTL). Neither MDIET nor PDIET altered the severity of Wooden Breast and White Striping (p ≥ 0.106). Overall, including 25OHD3 in either the maternal or broiler diet increased broiler meat yield.

Carcass Characteristics, Digestive System Traits of Spent Broiler Breeder and Dual-Purpose Hens.

Simple SummaryThe success achieved in the breeding of laying hens and broilers resulted in modern breeding systems. Obtaining meat from laying hens is not a good alternative to intensive broiler production. However, it can be profitable to use this type of production on organic or backyard farms. Dual-purpose hens can be an economical alternative in some production conditions. The aim of the study was to determine the differences between the features of carcasses and the digestive systems of dual-purpose hens and broiler breeders. The compared dual-purpose hens differed significantly (p < 0.05) from broiler breeders in terms of carcass weight and dimensions. Multipurpose hens had significantly lower (p < 0.05) percentages of breast muscles, leg muscles, skin with subcutaneous fat, wings, and carcass remainders. On the other hand, broiler breeders were characterized by significantly greater (p > 0.05) total intestinal length, duodenum, jejunum, ileum, and terminal intestine. Significant differences were noted in terms of the diameter of individual intestinal segments, with the exception of the iliac and cecum intestines. Broiler breeder hens also had a greater mass of internal organs compared to the dual-purpose layer hens.Raising dual-purpose hens for meat is believed to bring more economic benefits to farmers selling products directly to consumers. The aim of the study was to determine the differences between the carcass features and the digestive system of multipurpose hens and spent broiler breeders. In the experiment, 20 carcasses of 70-week-old Rosa 1 dual-purpose hens and 20 carcasses of 62-week-old Ross 308 broiler breeders were used. Measurements of the length of various sections of the intestine and the diameter of individual intestinal segments were made. During the gutting, proventriculus, gizzard, liver, heart, and spleen were separated and then weighed. Dual-purpose hens differed significantly (p < 0.05) from broiler breeders in weight and carcass dimensions. Multipurpose hens were characterized by significantly lower (p < 0.05) percentages of breast muscles, leg muscles, skin with subcutaneous fat, wings, and carcass remainders. Broiler breeders were characterized by significantly longer (p > 0.05) total intestinal length, duodenum, jejunum, ileum, and terminal intestine. Significant differences were confirmed in terms of the diameter of some segments of the intestine. Broiler breeder hens also had a greater mass of internal organs compared to the dual-purpose layer hens.

Impact of growth curve and dietary energy-to-protein ratio of broiler breeders on egg quality and egg composition

Egg characteristics have an impact on embryonic development and post-hatch performance of broilers. The impact of growth curve (GC) and dietary energy-to-protein ratio of broiler breeder hens on egg characteristics was investigated. At hatch, 1,536 pullets were randomly allotted to 24 pens in a 2 × 4 factorial dose-response design with 2 GC (standard growth curve = SGC or elevated growth curve = EGC (+ 15%)) and 4 diets, differing in energy-to-protein ratio (defined as 96%, 100%, 104% and 108% AMEn diet). Feed allocation per treatment was adapted weekly to achieve the targeted GC and to achieve pair-gain of breeders within each GC. Breeders on an EGC produced larger eggs (∆ = 2.3 g; P < 0.001) compared to breeders on a SGC. An exponential regression curve, with age (wk) of the breeders, was fitted to describe the impact of GC and dietary energy-to-protein ratio on egg composition. Yolk weight was 0.8 g higher for eggs from EGC breeders than from SGC breeders (, where was 22.1 and 22.9 for SGC and EGC, respectively; R2 = 0.97; P<0.001). An interaction between GC and dietary energy-to-protein ratio on albumen weight was observed (P = 0.04). Dietary energy-to-protein ratio did not affect albumen weight in SGC breeders (; R2 = 0.89), but for EGC breeders, a higher dietary energy-to-protein ratio resulted in a 0.9 g lower albumen weight from 96% AMEn to 108% AMEn (, where was 43.4, 43.2, 42.8, and 42.5 for 96% AMEn, 100% AMEn, 104% AMEn, and 108% AMEn, respectively; R2 = 0.86). Albumen DM content decreased linearly with an increased dietary energy-to-protein ratio, but this was more profound in EGC breeders (β = −0.03 %/% AMEn) than in SGC breeders (β = −0.01 %/% AMEn; P = 0.03). Overall, it can be concluded that an EGC for breeders led to larger eggs with a more yolk and albumen, whereas dietary energy-to-protein ratio had minor effects on egg composition.

Combined Maternal and Post-Hatch Dietary Supplementation of 25-Hydroxycholecalciferol Alters Early Post-Hatch Broiler Chicken Duodenal Macrophage and Crypt Cell Populations and Their Mitotic Activity.

The previous work has demonstrated that maternal supplementation of the circulating metabolite of vitamin D3 (D3), 25-hydroxycholecalciferol (25OHD3), enhances the immunocompetence of broiler chick offspring. In post-hatch broiler diets, 25OHD3 has been shown to affect intestinal morphology and improve the immune status of broilers. An experiment with a 2 × 2 factorial treatment arrangement was conducted to assess the effects of combining maternal (MDIET) and post-hatch (PDIET) dietary 25OHD3 inclusion on duodenal crypt and macrophage cell populations and mitotic activity in young broiler chickens. All diets were formulated to provide 5,000 IU of vitamin D. Broiler breeder hens were offered 1 of 2 MDIET: 5,000 IU D3 per kg of feed (MCTL) or 2,240 IU of D3 + 2,760 IU of 25OHD3 per kg of feed (M25OHD3) from week 25 to 41. Male broiler offspring (n = 480) hatched from eggs collected during week 41 of breeding age were allotted in raised floor pens (4 birds per pen from day 0 to 7 and individually allotted from day 8 to 21). Chicks were fed 1 of 2 PDIET (starter day 0 to 21): 5,000 IU D3 per kg of feed (PCTL) or 2,240 IU D3 + 2,760 IU 25OHD3 (P25OHD3). DUO samples (n = 12 birds per treatment per day) were collected on days 3, 6, 9, 12, 15, 18, and 21 for cryohistological and immunofluorescence analysis to facilitate the enumeration of the total macrophages, CD80+ macrophages (pro-inflammatory macrophages), and mitotically active cells (BrdU+) to calculate the proportion of proliferating cells (PPC) per duodenal crypt. Bird age impacted crypt PPC with the greatest PPC per duodenal crypt observed on days 3 and 9, and the lowest PPC per crypt was observed on day 21 (P < 0.0001). Broilers from the M25OHD3:PCTL treatment had a greater PPC (P =.002) than birds from the MCTL:PCTL treatment at day 3. An interaction among MDIET and PDIET was observed for proliferating macrophages at day 21 (P = 0.029) where M25OHD3:P25OHD3 birds had more proliferating macrophages than M25OHD3:PCTL-fed birds. These results indicate that combined MDIET and PDIET 25OHD3 supplementation may alter early post-hatch duodenal development and innate immunity.

Effect of early photostimulation at 15-weeks of age and everyday spin feeding on broiler breeder performance

To prevent broiler breeders from growing too quickly and becoming too large for optimum reproduction, their dietary intake is restricted. While current restricted feeding programs, such as skip-a-day feeding (SAD), improve the economic efficiency of broiler breeder operations, this management practice impacts bird welfare. There is an interest in finding strategies that could reduce the impact of feed restriction during broiler breeder rearing. This research investigated the effects of feeding pullets on an advanced growth curve for early photostimulation at 15 wk (15P) or standard growth curve for photostimulation at 21 wk (21P), using either an every-day-spin feeding program (EDS) or SAD feeding, on the reproductive parameters of broiler breeder hens in a 2 × 2 factorial arrangement. Overall, advancing the growth curve (15P) decreased blood corticosterone levels compared to 21P, but EDS resulted in higher blood corticosterone levels compared to SAD. At the end of rearing in both 15P and 21P, EDS pullets weighed less than SAD pullets. The onset of egg production was 20 and 24 wk of age for the 15P and 21P hens, respectively. Despite an earlier onset, 15P hens did not produce more eggs than 21P hens through 65 wk of age. Egg weight was reduced for 15P compared to 21P until 30 wk of age. The 15P hens had a greater number of double yolk eggs than the 21P hens. Fertility and hatch were not impacted by the advanced growth curve and early photostimulation. Although the current research indicates the potential to reduce feed restriction associated welfare issues by rearing broiler breeder pullets for an earlier photostimulation onset, further research in needed to determine if this management technique can be improved to optimize hen reproductive efficiency.

The effects of feed restriction, time of day, and time since feeding on behavioral and physiological indicators of hunger in broiler breeder hens

Broiler breeder chickens are commercially feed restricted to slow their growth and improve their health and production, however, there is research demonstrating that this leads to chronic hunger resulting in poor welfare. A challenge in these studies is to account for possible daily rhythms or the effects of time since last meal on measures relating hunger. To address this, we used 3 feed treatments: AL (ad libitum fed), Ram (restricted, fed in the morning), and Rpm (restricted, fed in the afternoon) to control for diurnal effects. We then conducted foraging motivation tests and collected home pen behavior and physiological samples at 4 times relative to feeding throughout a 24-h period. The feed treatment had the largest influence on the data, with AL birds weighing more, having lower concentrations of plasma NEFA, and mRNA expression of AGRP and NPY alongside higher expression of POMC in the basal hypothalamus than Ram or Rpm birds (P < 0.001). R birds were more successful at and had a shorter latency to complete the motivation test, and did more walking and less feeding than AL birds in the home pen (P < 0.01). There was little effect of time since last meal on many measures (P > 0.05) but AGRP expression was highest in the basal hypothalamus shortly after a meal (P < 0.05), blood plasma NEFA was higher in R birds just before feeding (P < 0.001) and glucose was higher in Ram birds just after feeding (P < 0.001), and the latency to complete the motivation test was shortest before the next meal (P < 0.05). Time of day effects were mainly found in the difference in activity levels in the home pen when during lights on and lights off periods. In conclusion, many behavioral and physiological hunger measures were not significantly influenced by time of day or time since the last meal. For the measures that do change, future studies should be designed so that sampling is balanced in such a way as to minimize bias due to these effects.

Feeding level is associated with altered liver transcriptome and follicle selection in hen†.

Genetic selection for particular traits in domestic animals may have altered the optimal feedback regulation among systems regulating appetite, growth, and reproduction. Broiler breeder chickens have been selected for fast and efficient growth and, unless feed restricted, consume excessively resulting in poor reproductive efficiency. We examined the effect of dietary treatment in full-fed and restricted-fed broiler breeder hens on ovarian responses, liver morphology, and transcriptome associated with reproductive function. Although full-fed broiler breeder hens had lower egg production (P < 0.01), the total number of ovarian follicles >8mm (P < 0.01), 6-8mm (P < 0.03), and 3-5mm (P < 0.04) were greater in full-fed hens compared to restricted-fed hens. There was a large amount of lipid accumulation in the liver of full-fed hens and differential gene analysis yielded 120 genes that were differentially expressed >2-fold in response to feeding level (P < 0.01; false discovery rate< 0.05). Elevated T3 may indicate that general metabolism was affected by diet and GHR (P < 0.01) and insulin like growth factor 1 (IGF1) (P < 0.04) mRNA expression were both greater in the liver of full-fed hens as compared to restricted-fed hens. It is likely that selection for increased growth, associated with enhanced activity of the IGF1 system, has altered nutritional coupling of feed intake to follicle development.

Effect of Temperature and Feeding Time on Shell Thickness

The objective of this study was to assess the effect of ambient temperature (T) and feeding time (FT) on eggshell thickness (ST) and egg weight (EW) of broiler breeder hens. Thirty 44-week-old Ross 308 broiler breeder hens were randomly distributed into six environmentally controlled chambers and kept in individual cages. Three thermal treatments were applied: 20 ºC (T1), cyclic 20-30 ºC (30 °C between 1000 h and 1800 h) (T2), and 30 ºC (T3). Birds received 180 g of commercial breeder food at 0730 h (FT1) or 1530 h (FT2). There were two replications per treatment and egg collection was performed for seven days. The eggs from the birds at T1 and T2 fed at 1530 h (T1FT2 and T2FT2 respectively) were significantly heavier than those laid by hens at the same T when fed in the morning (T1FT1 and T2FT1); eggs from T1FT1 and T2FT1 were heavier than the eggs from T3, but there were no significant differences between them. Both T and FT had significant effects on ST, but no significant interactions were found. Birds fed at 1530 h had the highest ST, whilst birds at T3 showed the lowest. Birds on T2 produced the thickest shells due to a higher ST in birds fed at 1530 h. Birds fed at 1530 h consumed their food between 1800 h and 2030 h, resulting in a higher dietary Ca2 + available during shell mineralization. The effect of FT and biphasic T treatments on ST in feed restricted broiler breeders should be interpreted considering the length of the high T phase to the actual food consumption time.

Carcass Characteristics and Physical Meat Quality Properties of Spent Broiler Breeder Hens and Commercial Spent Layer Hens

This study was conducted to investigate the carcass characteristics and physical meat quality properties of spent broiler breeder, commercial spent white, and commercial spent brown layer hens. Ten samples from each genotype were analyzed for muscle pH, meat colour characteristics, and carcass yield performance. Meat colour analyses were performed on skinned and skinless breast and thigh meat samples, while meat pH was measured on skinned meat samples. The percentage of the breast meat of carcass was significantly higher for broiler breeder hens than for white and brown layer hens (P&amp;lt;0.001), while wing meat percentage was significantly lower for these birds (P&amp;lt;0.005). There were significant differences in the lightness value of skinned layer breast meat (P&amp;lt;0.016). Skinned redness (P&amp;lt;0.001), skinned chroma (P&amp;lt;0.001), skinless lightness (P&amp;lt;0.001), and skinless chroma (P&amp;lt;0.002) values of thigh meat were significantly affected by the genotype of spent layer hens. The spent breeder hens had significantly lower pH levels for leg and breast meat (P&amp;lt;0.001). In conclusion, it seems that the carcass and meat quality characteristics of the spent laying hens differed. The spent layer meat might be an alternative meat for consumer as breast and leg meat or processed meat.

Clinical study to evaluate fenugreek (Trigonella foenum-graecum L.) seed and galactomannan depleted fenugreek residue on blood glucose level in broiler breeder hens

A study was undertaken to evaluate the blood glucose level of broiler breeder hens supplemented with fenugreek seed and Galactomannan Depleted Fenugreek Residue (GDFR). The evaluation was based on series of biological trial and chemical analyses. In a broiler breeder trial, 4320 numbers of thirty three weeks old Vencobb 400-Y strain were distributed randomly to nine treatments and each treatment had five replicates and each replicate had 96 birds. Supplementation of fenugreek seed or GDFR up to 4% in broiler breeder decreased the blood glucose level. The blood glucose level was decreased at all level of fenugreek seed supplementation and when GDFR was supplemented 2% and above.

Efficiency of ultraviolet light for disinfection of fertile broiler eggs

ABSTRACT This study aimed to evaluate the effectiveness of ultraviolet light in reducing bacterial load of eggshells and the impact of experimental disinfection on hatching, embryo mortality, and time-borne distribution using broiler breeder hens of different ages (38, 42, and 48 weeks old). Fertile eggs were subjected to different exposure periods (5, 7, and 9 minutes) of UV light (UV-C) with a 254 nm wavelength. For controls, eggs disinfected with paraformaldehyde (5.3 g/m3) and eggs not disinfected (NC). After subjection to disinfection protocols, the eggs were placed into sterile plastic bags containing 20 mL of peptone saline solution (0.1% m:v) and massaged for 1 minute to release the bacterial load. Aliquots of this solution were incubated in specific medium for bacterial growth for 48 hours at 37ºC for subsequent CFU counts. To evaluate the effects of disinfection on production, eggs previously disinfected by UV-C (9 min) and paraformaldehyde and NC eggs were candled between incubation days 10 and 13 and at the end of the incubation period to assess embryonic mortality. Hatchability distribution was performed every 8 hours. The 9 minutes 254nm UV-C light exposure was able to disinfect viable eggs and matched the effectiveness of the paraformaldehyde technique.

Rearing system, utility type and hens’ age as a factor modifying lysozyme's hydrolytic activity

&lt;b&gt;The aim of study was to analyze the impact of the utility type, age and rearing system of hens on egg quality traits and the enzymatic activity of egg albumen lysozyme (LA). The material consisted of 120 eggs from two genotypes of hens: Hy-Line Brown and parent stock of Ross 308. Laying-type hens were reared divided into 2 systems: intensive (litter, balanced feed) and extensive (open runs, farm fodders). The second source of material was hens age (29, 33, 41, 53 and 70 wks) of the parent stock.. Stocks were differentiated in terms of the hens age (29, 33, 41, 53 and 70 wks). Eggs quality traits and LA were evaluated. The LA of was higher in eggs from laying than from broiler breeder hens. It decreased with the hens’ age and increased with albumen mass, it was also positively correlated with albumen height and Haugh's units. The rearing system considerably modified mass of egg and albumen and the dense albumen height, but does not affect the LA.

Ad Libitum Feeding in Broiler Breeder Hens Alters the Transcriptome of Granulosa Cells of Pre-Hierarchal Follicles.

Simple SummaryBroiler breeds of chickens have been bred for fast growth and feed efficiency, while laying breeds have been bred for optimal egg production. As a consequence of intense selective breeding in broiler breeds, egg production is greatly reduced and leads to inefficient reproduction. One strategy used by producers to improve egg production in broiler breeds has been to limit feed allowance. In this study, we aimed to identify differences in ovarian gene expression between broiler breeder hens fed high (ad libitum) and low feed allowances. Several differences in gene expression were identified, which may explain the decreased egg production seen in broiler breeder hens fed ad libitum. These results inform the poultry industry on the biology of broiler breeder hens fed ad libitum and how levels of high feed intake affect reproductive efficiency.Intense selective breeding of chickens has resulted in suboptimal egg production in broiler breeder hens. This reproductive phenotype is exacerbated by ad libitum feeding, which leads to excessive and disorganized follicular growth. One strategy used to improve broiler breeder hens’ reproductive efficiency is restricted feeding. In this study, we sought to identify transcriptional changes, which translate the level of dietary intake into increased follicle selection. Broiler breeder hens (n = 16 per group) were raised according to commercial guidelines until 28 weeks of age and then randomly assigned to an ad libitum diet (FF) or continued on a restricted diet (RF) for 6 weeks. Following dietary treatment, FF hens (n = 2) with excessive follicle selection and RF hens (n = 3) with normal follicle selection were selected for RNA-sequencing. Transcriptomes of granulosa cells from 6–8-mm follicles were sequenced to identify transcriptional differences in the follicle population from which selection was made for the preovulatory stage. Differential expression analysis identified several genes known to play a role in follicle development (CYP11A1, STAR, INHA, and INHBB) that are upregulated in FF hens. These changes in gene expression suggest earlier granulosa cell differentiation and steroidogenic competency in the granulosa layer from FF hens.

Effects of zinc dosage and particle size on gut morphology, tight junctions and TNF-α expression in broiler breeder hens.

This study was performed to evaluate the effects of different amounts and particle size of zinc oxide (ZnO) on villus height (VH), villus width (VW), crypt depth (CD) and VH to CD ratio (VH: CD), and expression of zonula occludens-1 (ZO-1), occludin (OC) and tumour necrosis factor-α (TNF-α) in broiler breeders. A total of 350 (Ross 308) broiler breeder hens of 54weeks randomly assigned to seven treatments, included control basal diet (C) without added Zn, C+ 100, and 130mg Zn per kg of diet from Large (L) (100-1000nm) and Small (S) (<100nm) particle size ZnO (LZnO100 and 130; SZnO100 and 130), C and SZnO100 challenged with lipopolysaccharide (C+LPS and SZnO100+LPS). Each diet was fed to five replicates consisting of ten birds each. The middle part of the duodenum, jejunum and ileum was used for morphological assessments. To assess the gene expression of ZO-1, OC and TNF-α in the jejunum samples were excised. Results showed that the supplementing 130ppm SZnO increased VH:CD in the duodenum (p<0.05). VW in the duodenum and all the evaluated morphometric indices in jejunum and ileum were not affected by the dietary treatment (p>0.05). ZO-1mRNA abundance in C+LPS group compared to SZnO100+LPS group was significantly decreased and increased by LPS and SZnO100 respectively. The SZnO-100 increased OC gene expression in compare to C+LPS group. The expression of TNF-α in C+LPS treatment was higher than other groups (p<0.05). The lowest and the highest litter moisture and foot-pad dermatitis (FPD) were observed in LZnO-130 and C treatments respectively (p<0.05). Improving the physical properties of ZnO affect on VH:CD. Broiler breeder diet with ZnO enhance ZO-1, OC and mitigate TNF-α gene expression in jejunum maintenance of gut health in broiler breeders.

Effects of Nutritional Restriction during Laying Period of Fat and Lean Line Broiler Breeder Hens on Meat Quality Traits of Offspring.

Simple SummaryThe meat quality of livestock products is widely appreciated. Maternal nutrition can affect the deposition of nutrients in eggs, and then change the apparent metabolism, development process, and performance of offspring. Our research indicated that meat quality traits are also affected by maternal nutritional level and are related to the nutritional requirements of different genotypes. Some of the effects disappeared at the end of the growth stage. These situations remind poultry producers to consider the impact of feed restrictions on the quality of meat for future generations.The offspring meat quality of hens undergoing a 25% dietary restriction treatment during the laying period were evaluated in fat and lean line breeder. A total of 768 female birds (384/line) were randomly assigned to four groups (12 replicates/group, 16 birds/replicates). Maternal feed restriction (MFR) and normal started at 27 weeks of age. Offspring broilers were fed ad libitum. The offspring meat quality traits and muscle fiber morphology in different periods were measured. At birth, significant interactions were found on breast muscle fiber morphology (p < 0.05). At 28 days, MFR decreased breast water content and increased thigh crude fat content, and significant interactions were observed on breast crude fat and protein contents (p < 0.05). At 56 days, MFR affected morphology of peroneus longus muscle tissue, and significant interactions were found on thigh redness at 48 h and amino acid contents in breast and thigh muscle (p < 0.05). Overall, MRF may lead to offspring birth sarcopenia. Such offspring grow more easily to deposit fat in a nutritious environment, but they will self-regulate adverse symptoms during growth and development. The two lines respond differently to maternal nutritional disturbance due to different nutritional requirements and metabolic patterns.

Transgenerational effects of innate immune activation in broiler breeders on growth performance and immune responsiveness

The impact of transgenerational effects on growth performance and immunity has not yet been studied extensively within the poultry husbandry sector. An important factor is the impact of the hens on the physical well-being and fitness to the environment of the offspring. This study is the first to investigate the effect of stimulating the maternal innate immune system with lipopolysaccharides (LPS) or β-glucan on growth performance and immune responses in the next generation. Transgenerational effects and consequences of these maternal treatments were further examined using a necrotic enteritis (NE) challenge model in the offspring. We show that offspring of LPS-treated broiler breeders have a higher feed efficiency from 14 to 21 days of age, that is, the period just after the NE challenge. Moreover, more broiler chickens with intestinal lesions after the NE challenge were found in the offspring of the LPS-treated broiler breeders. Both the LPS and β-glucan maternal treatments resulted in transgenerational effects on blood-derived monocytes by showing a tendency of decreased IL1β mRNA levels after ex vivo LPS stimulation. These data are a first indication that broiler breeder hens can affect immune responsiveness and feeding efficiency of their offspring in a transgenerational manner.