Brine Shrimp Lethality Test Research Articles

Toxicity Effect Test of Matoa (Pometia pinnata) Plant Fractions as Raw Materials for Anticancer Drugs

Background:The prevalence of cancer in Indonesia increases every year. Medical treatments such as chemotherapy, surgery, and radiation require significant costs with considerable side effects. Therefore, there is a need for chemopreventive agents from natural sources such as plants. One plant with potential as a chemopreventive agent is the matoa tree (Pometia pinnata), which is empirically used to treat suppurating wounds.Objectives: This research aims to test the toxicity of leaf and stem bark fractions of matoa using shrimp larvae Artemia salina Leach. Methods: The parameter for this study is the LC50 value. The extraction method involves successive maceration using solvents based on increasing polarity, starting with n-hexane, chloroform, ethyl acetate, and methanol. Phytochemical screening is conducted using colour tests, and toxicity is assessed through the brine shrimp lethality test. The research indicates that all fractions of matoa leaves and stem bark are toxic with respective LC50 values for leaf fractions: n-hexane (394.8 µg/mL), chloroform (244.3 µg/mL), ethyl acetate (180.6 µg/mL), and methanol (303.2 µg/mL). Stem bark fractions exhibit LC50 values in the order of n-hexane (203.9 µg/mL), chloroform (244.3 µg/mL), ethyl acetate (144.8 µg/mL), and methanol (58.3 µg/mL). Conclusions: All fractions fall into the toxic category and have the potential as raw materials for anti-cancer drugs.

Extraction of Sulfated Polysaccharides from Ulva sp. Using Acid and Toxicity Testing with the Brine Shrimp Lethality Test (BSLT)

This study investigates the extraction of sulfated polysaccharides from Ulva sp. at pH 3 and evaluates their toxicity using the Brine Shrimp Lethality Test (BSLT) with Artemia salina. Artemia salina is utilized for its rapid life cycle and sensitivity to various chemical compounds, making it an effective bioassay organism for assessing the toxicity of natural extracts. The extraction process was conducted at both hot (80°C) and cold (room temperature) conditions, with the hot extraction yielding higher amounts of polysaccharides. The results indicated a wet extraction yield of 890 mL at 80°C and 800 mL at room temperature, while the highest dry weight yield was achieved at 80°C (0.57 g). The toxicity assessment revealed an LC50 value of 15,815.85 ppm, classifying the sulfated polysaccharides as non-toxic to Artemia salina. Maintaining optimal water quality parameters, including temperature, salinity, pH, and dissolved oxygen levels, is essential for the successful cultivation of Artemia salina. Furthermore, the acidic extraction pH significantly influences the structure and chemical properties of the resulting polysaccharides, emphasizing the importance of this parameter in future applications. These findings support the potential use of sulfated polysaccharides from Ulva sp. as a safe and effective feed ingredient in aquaculture.

Assessment of Fungal Contamination and Their Toxigenic Potential of Farmed Fish in Western Algeria

Fish and fish products have been documented as a major source of pathogens, with fungi playing a key role. In this paper, a survey was conducted to determine the presence of fungi in fish reared in various aquaculture farms in western Algeria, namely Nile Tilapia (Oreochromis niloticus), gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax). The search for micromycetes was carried out on different parts of the body: skin and flesh, gills, and intestines. A total of 1937 fungal strains were revealed and divided into 10 genera of filamentous fungi, one genus of yeast, and other yeasts that have not yet been determined. Penicillium was the most common genus, representing 41.56% of the fungal population, fol-lowed by Rhodotorula (17.60%), and Aspergillus (15.95%). The greatest number of isolates was identified in the gills of gilthead sea bream, while the lowest number was isolated from sea bass intestines. Secondly, we sought to highlight the toxigenic potential of the fungal strains isolated, using the brine shrimp lethality test. Five of the fungal strains tested, belonging to the two genera Penicillium and Aspergillus, showed toxic potential for Artemia salina. The highest toxicities were observed mainly by strains of Aspergillus parasiticus (APAR001), Aspergillus terreus (ATER002), and Aspergillus niger (ANIG001) respectively. Penicillium chrysogenum (PCHR002) showed the lowest activity. These results confirm the infection of farmed fish by toxigenic fungal communities, which can present a real health hazard to consumers. Regular monitoring and compliance with fish health management practices are recommended.

Phytochemical Screening, Antioxidant, Cytotoxic, Analgesic, Antidiarrheal Activity and GC-MS Analysis of the Leaves of Stachyphrynium Placentarium

The goal of the present study is to analyze the phytochemicals, antioxidants, cytotoxic, analgesic and anti-diarrheal properties of the leaves of Stachyphrynium placentarium methanol extract as well as its dichloromethane soluble fraction using GC-MS technique. The leaves of the S. placentarium were extracted with methanol and designated as SPM. Phytochemicals investigation exhibited the presence of several important secondary metabolites. Antioxidants such as total phenolic content, flavonoids content, total antioxidant, reducing power capacity and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay SPM revealed non-significant antioxidant properties. The cytotoxicity study of the extract showed significant cytotoxicity (LC50 35.1093 µg/mL) compared to standard vincristine sulfate (LC50 0.482 µg/mL). Analgesic study of S. placentarium by hot plate model indicates both the doses exhibited significant central analgesic activity compared with the control. The maximum analgesic activity was observed at 90 min. SPM (500 mg/kg) is 60.21 %. SPM250 also showed a significant percentage of elongation or latency from 30 to 150 min 57.00, 59.64, 59.88, 58.07 and 53.71 %, respectively 30 min time interval. In the acetic acid writhing test, S. placentarium significantly outperformed the control and standard (SPM250 - 52.27 %, SPM500 - 34.09 and STD - 27.27 %), showing a significant increase in peripheral analgesic activity (p < 0.001). Antidiarrheal activity of SPM showed that SPM250 and SPM500 substantially decreased the feces in castor oil-induced diarrhea (p < 0.05). According to the study, the incidence and severity of diarrhea are significantly reduced by SPM500. Four chemicals are found in the dichloromethane-soluble portion of the methanol extract of S. placentarium leaves, according to GC-MS studies such as Octadecane, 6-methyl- (1.326 %), Dodecane (1.02 %), 1-Iodo-2-methylundecane (0.47 %), tetradecanoic acid 12-methyl, methyl ester (72.64 %). Tetradecanoic acid exhibits anti-inflammatory, anticancer, 5-reductase inhibitory, antioxidant, hemolytic and antifibrinolytic properties. HIGHLIGHTS The phytochemicals, antioxidants, cytotoxic, analgesic and anti-diarrheal properties of Stachyphrynium placentarium methanol extract (SPM) leaves. The dichloromethane soluble fraction is examined using Gas chromatography–mass spectrometry (GC-MS) and identified Octadecane, 6-methyl-, Dodecane, 1-Iodo-2-methylundecane, tetradecanoic acid 12-methyl, methyl ester. The brine shrimp lethality test exhibited that SPM has significant cytotoxicity (LC50 35.1093 µg/mL) compared to vincristine sulphate (LC50 0.482µg/mL), the extract showed significant cytotoxicity (LC50 35.1093 µg/mL). S. placentarium at both dosages (250 and 500 mg) provided significant central analgesia compared to the control in a hot plate model. In the acetic acid writhing test, S. placentarium showed significantly higher peripheral analgesic action (SPM250 - 52.27, SPM500 - 34.09 and STD - 27.27 %) compared to control and standard. In castor oil-induced diarrhoea, SPM250 and SPM500 (250 and 500 mg) showed significantly reduced faeces production (p < 0.05). GRAPHICAL ABSTRACT

Metabolit Sekunder dan Uji Bioaktivitas Sitotoksik Ekstrak Buah Sumpit (Brucea javanica (L.) Merr) terhadap Artemia salina Leach

Research carried out with the title of Secondary Metabolite Identification and bioactivity Fruit Extract Sumpit (Brucea javanica (L) Merr) to the shrimp larvae Artemia salina Leach. This study aims to identifying classes of secondary metabolites as well as test the bioactivity of secondary metabolites sumpit. Identifikasi fruit extracts performed to test the Chopsticks fruit extracts and fractions against reagent, while the bioactivity test was conducted using Brine Shrimp Lethality Test (BSLT). Data were analyzed using the method of Reed and Muench to get Lethality Concrentation value 50% (LC50). The results showed that the fruit extract Sumpit contain the alkaloid class of secondary metabolites, karatenoid, saponins, tannins, phenolic compounds, steroids, and terpenoids. Sumpit fruit bioactivity test results had LC50 values of the methanol extract of 3.10 ppm, n-hexane fraction of 2.09 ppm, 0.79 ppm ethyl acetate fraction and n-butanol fractions of 0.74 ppm.

Therapeutic potential of Zanha africana (Radlk.) Exell: antimycobacterial activity and safety evaluation

BackgroundZanha africana (Radlk.) Exell extracts are commonly utilized in Tanzania and other countries for treating conditions associated with HIV and AIDS, including tuberculosis (TB). In Tanzania, it is listed among the most frequently traded plant species for medicinal use in different marketplaces. However, limited literature exists regarding its antimycobacterial properties and safety profile. ObjectiveThis study aimed to evaluate Z. africana ethanolic extract for safety and antimycobacterial activity. MethodsThe antimycobacterial activity of the crude extracts was assessed using the broth microdilution method against various non-pathogenic mycobacteria strains including Mycobacterium aurum (MA), Mycobacterium madagascariense (MM), and Mycobacterium indicus pranii (MIP). Bioassay-guided fractionation was employed to isolate the active compounds. The safety profile of the crude extract was investigated through cytotoxicity assay on peripheral blood mononuclear cells (PBMCs) and the brine shrimp lethality test. ResultsThe 80% ethanolic extracts of Z. africana demonstrated activity against all tested non-pathogenic mycobacteria. The antimycobacterial activity of ethanolic extract of the stem bark was 625 µg/mL, 313 µg/mL and 125 µg/mL against MA, MM, and MIP, respectively. The most potent fraction was the ethyl acetate fraction, from which two active compounds were isolated: proanthocyanidin A2; Compound (1) and its monomer epicatechin, Compound (2). Compound (1) exhibited higher antimycobacterial activity compared to compound (2) with minimum inhibitory concentrations (MICs) of 19.50 µg/mL and 12.98 µg/mL against MM and MIP respectively. The ethanolic extract did not demonstrate toxicity on PBMCs and brine shrimps. ConclusionThis study demonstrates that Z. africana extracts and the isolated compounds possess antimycobacterial activity. This provides evidence supporting the traditional use of Z. africana extracts for TB management. Further research is recommended to comprehensively evaluate the efficacy and safety of the plant constituents using different models.

Clinacanthus nutans crude polysaccharide extract as a green platform for microwave-assisted synthesis of silver nanoparticles: Optimization, characterization, and evaluation of bioactivities

Clinacanthus nutans (C. nutans) is a plant in tropical Asia with proven biological activities. The optimized extraction method of C. nutans crude polysaccharide (CNP) uses water in the presence of an ultrasound-assisted mechanical method (UL_CNP). However, the use of UL_CNP for the synthesis and optimization of silver nanoparticles (AgNP), particularly their anticancer and photocatalytic properties, remains unexplored. Hence, this research aimed to employ a green method using UL_CNP and silver nitrate to produce AgNP (UL_AgNP) with a small size and assess its potential toxicity, anticancer, and photocatalytic activities. The synthesis condition was optimized using the Box–Behnken design method. The synthesized UL_AgNP showed the surface plasmon resonance peak at 458 nm. The optimized synthesis condition produced spherically shaped UL_AgNP with a size of 5.21 ± 1.92 nm and a zeta potential of −26.33 ± 0.93 mV. An X-ray diffraction analysis exhibited intense Bragg's reflection peaks at (111), (200), (220), and (311), having a face-centered cubic structure of AgNP. Attenuated total reflectance-Fourier-transform infrared spectroscopy and energy-dispersive X-ray spectroscopy further confirmed the presence of silver in the synthesized UL_AgNP. The brine shrimp lethality test of UL_AgNP reported a lethal concentration 50 value of <7.8 μg/mL after 24 h. The UL_AgNP exhibited antiproliferative activity against MCF-7 cells with a half-maximal inhibitory concentration value of 4.96 ± 0.31 μg/mL by inducing S-phase cell cycle arrest, apoptotic effect, and reduction of cell migration. Furthermore, UL_AgNP proved its efficient photocatalytic activity against methylene blue dye (50.22 % ± 0.06 %, after 10 min at a concentration of 50 μg/mL). Therefore, the UL_AgNP exhibited promising antiproliferative activity against MCF-7 cells, highlighting their potential as a therapeutic agent. Further investigations are needed to elucidate the precise mechanism of their action.

Antibacterial and Antibiofilm Properties of Native Australian Plant Endophytes against Wound-Infecting Bacteria.

The wound management field faces significant challenges due to antimicrobial resistance (AMR) and the complexity of chronic wound care. Effective wound treatment requires antimicrobial dressings to prevent bacterial infections. However, the rise of AMR necessitates new antimicrobial agents for wound dressings, particularly for addressing bacterial pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Endophytic fungi, known for producing diverse bioactive compounds, represent a promising source of such new agents. This study tested thirty-two endophytic fungi from thirteen distinct Australian native plants for their antibacterial activity against S. aureus. Ethyl acetate (EtOAc) extracts from fungal culture filtrates exhibited inhibitory effects against both methicillin-sensitive S. aureus ATCC 25923 (MIC = 78.1 µg/mL) and MRSA M180920 (MIC = 78.1 µg/mL). DNA sequence analysis was employed for fungal identification. The most active sample, EL 19 (Chaetomium globosum), was selected for further analysis, revealing that its EtOAc extracts reduced S. aureus ATCC 25923 biofilm formation by 55% and cell viability by 57% to 68% at 12 × MIC. Furthermore, cytotoxicity studies using the brine shrimp lethality test demonstrated low cytotoxicity up to 6 × MIC (25% mortality rate) with an LC50 value of 639.1 µg/mL. Finally, the most active sample was incorporated into polycaprolactone (PCL) fiber mats via electrospinning, with resultant inhibition of S. aureus species. This research underscores the potential of endophytic fungi from Australian plants as sources of substances effective against common wound pathogens. Further exploration of the responsible compounds and their mechanisms could facilitate the development of wound dressings effective against MRSA and innovative biofilm-resistant electrospun fibers, contributing to the global efforts to combat AMR.

Toxicity Test, Antioxidant Activity, and Determination of Total Flavonoid Content (TFC) of N-Hexane and Ethyl Acetate Fraction from Uncaria Nervosa Elmer (Bajakah) Root Wood

Uncaria nervosa Elmer (local name: Bajakah) is one of the medicinal plants used as a cancer drug by the people of Muara Badak, East Kalimantan. The purpose of this study was to determine the total flavonoid content (TFC), toxicity, and antioxidant activity of the n-hexane (NH) and ethyl acetate (EA) fractions of Uncaria nervosa root wood. Determination of total flavonoids was carried out using a UV-Vis spectrophotometer with quercetin as a standard, toxicity test was carried out using the brine shrimp lethality test (BSLT) method, and antioxidant test using the DPPH radical reduction method. Based on the results of this study, the total flavonoid content (TFC) of NH and EA fractions were 192 and 425 mg QE/g extract, respectively. Based on the results of the toxicity test on Artemia salina L. shrimp, the LC50 values were 11.91 ppm for NH and 33.19 ppm for EA. These LC50 values indicate that NH is very toxic and EA is toxic against Artemia salina L. While the antioxidant test results showed that EA was very significant as an antioxidant and NH was moderate with IC50 values of 17.54 ppm and 75.93, respectively.

Acute toxicity, secondary metabolites, and antioxidant activity of Macaranga tanarius from post-coal mining and non-mining areas in East Kalimantan, Indonesia.

Coal plays a crucial role in Indonesia's foreign exchange and East Kalimantan's revenue sharing, yet its environmental impacts, including soil acidification, raises concerns. Reclamation measures involve revegetation with pioneer plants such as Macaranga sp., known for their medicinal properties. However, the pharmacological properties of these plants are influenced by secondary metabolites, which depend on soil parameters such as pH and nutrient levels. The aim of this study was to evaluate the acute toxicity, secondary metabolites, and antioxidant activities of Macaranga tanarius leaf extracts from post-coal mining area (MTPCMA) and non-mining area (MTNMA) alongside soil parameters. Acute toxicity of M. tanarius leaf extracts and soils were assessed using the brine shrimp lethality test (BSLT). Phytochemical screening was done using thin-layer chromatography (TLC), determining total phenolic (TPC) and flavonoid content (TFC). The DPPH radical scavenging assay was used to assess the antioxidant activity. A comparative analysis between MTPCMA and MTNMA was conducted using Student t-test. The data showed no significant difference in toxicity between MTPCMA and MTNMA leaf extracts (LC50 of 100-1000 µg/mL) (p=0.062), and soils from both areas were non-toxic (LC50 of >1000 µg/mL). Although heavy metal concentrations were higher in PCMA than in NMA soil (p<0.001), secondary metabolite compounds and TFC in both extracts were not significantly different (p=0.076). Both extracts contained flavonoids and polyphenols with antioxidant activity and terpenoids without antioxidant activities. The DPPH radical scavenging test suggested insignificant antioxidant activity between MTPCMA and MTNMA extracts (p=0.237). In conclusion, non-toxic soils in post-mining land and insignificant differences between MTPCMA and MTNMA extracts suggest good soil nutrient availability, highlighting the success of land recovery after 10 years of revegetation with M. tanarius.

Antioxidant Capacity, Toxicity, Phytochemicals, Total Alkaloids and Phenolic Levels Bamboo Shoot Extract ( Bambusa vulgaris )

The imbalance between reactive oxygen species (ROS) and endogenous antioxidants can cause various diseases, so exogenous antioxidants from plants such as bamboo shoots (Bambusa vulgaris) are needed. This study explored the antioxidant capacity, toxicity, and phytochemical content of bamboo shoot extracts. Using an in vitro experimental design, we analyzed bamboo shoot extracts for phytochemical content, antioxidant capacity using the DPPH method, and toxicity using the Brine Shrimp Lethality Test (BSLT). The research was conducted from December 2020 to June 2021 at the Biochemistry and Molecular Biology Laboratory, Tarumanagara University. Data were analyzed using Graph Pad Prism V.8 software with the level of significance set at p&lt;0.05. Phytochemical test results showed the presence of alkaloids, anthocyanins, betasianins, cardiac glycosides, coumarins, flavonoids, glycosides, phenolics, quinones, saponins, terpenoids, and tannins. The bamboo shoot extract showed antioxidant capacity with IC50 value of 673.54 µg/mL, total phenolic content of 2923.65 µg/mL, and total alkaloid content of 7.34 µg/mL. The toxicity test showed an LC50 value of 362.16 µg/mL. The bamboo shoot extract showed potential as an antioxidant and anti-mitotic agent, although its antioxidant capacity was lower than that of ascorbic acid. The presence of various secondary metabolites contributes to its bioactive properties. Further research is needed to explore its potential applications in preventing diseases caused by free radicals. Bamboo shoot extract contains significant amounts of phenolic compounds and other phytochemicals, which exhibit antioxidant and toxic properties, making it a potential natural source for antioxidant agents.

Phytochemical screening, assessment of biological activity and nutritional value of Sudanese Hibiscus sabdariffa L. seeds

Medicinal plants are widely used in traditional cultures all over the world and they are becoming increasingly popular in modern society as natural alternatives. This research aimed to conduct phytochemical screening; assess of antimicrobial and antioxidant activities; evaluate toxicity and detect the nutritional value of Hibiscus sabdariffa L. seeds. The Phytochemical screening was carried out using standard procedures. The samples were extracted using hexane and absolute ethanol respectively. The extracts were evaluated for their effectiveness against microorganisms using the disc diffusion method and the antioxidant potential was measured by the DPPH assay. The proximate analysis of seed powder and mineral composition was determined using standard procedures. Toxicity was evaluated by the brine shrimp lethality test and the LD50 was calculated. Phytochemical screening revealed the presence of alkaloids, saponins and flavonoids, while tannins and anthraquinones were not detected. Generally, the hexane extract showed higher antibacterial activity than the ethanolic extract against 4 bacteria. Both extracts exhibited antifungal activity against Candida albicans ranging from 12-17 mm. The hexane extract showed higher antioxidant activity than the ethanolic extract (60 % and 47 % respectively). Both extracts were non-toxic against brine shrimps, suggesting that they are safe for medicinal use. The results of the proximate analysis showed that the seeds contain high amounts of fat (34.19 %) and protein (33.25 %). The seeds of H. sabdariffa contained considerable amounts of some important elements, while lead was not detected. The findings of this study revealed that H. sabdariffa seeds are a rich source of secondary metabolites and nutritional value and could be used for pharmaceutical preparation and drug development.

GC-MS analysis and pharmacological potentiality of Lasia spinosa (L.) Thwaites leaves and fruit extracts: an in vitro and in silico studies.

Lasia spinosa (L. spinosa) is widely used in Asian countries for treating various diseases and as a vegetable, yet its bioactive properties remain under-researched. It is traditionally utilized in Ayurveda and the AYUSH system of medicine for its medicinal properties, and commonly used to treat digestive disorders, respiratory issues, and inflammatory conditions. This study aims to identify the phytochemicals in L. spinosa leaves and fruit extracts and evaluate their biological activities. Phytochemicals in methanol extracts of L. spinosa fruits and leaves were identified by GC-MS analysis. Antioxidant and cytotoxic activities were assessed using the DPPH free radical and nitric oxide (NO) scavenging assay and brine shrimp lethality test. Antibacterial activity was evaluated against Shigella boydii, Shigella flexneri, Streptococcus iniae, and Streptococcus dysgalactiae, while antifungal properties were tested against Cercospora beticola and Rhizoctonia solani. Molecular docking was conducted to predict the effectiveness of L. spinosa phytochemicals against NADPH oxidase and the Shigella effector OspG. Nine compounds were detected from both extracts. The methanol leaves extract exhibited superior antioxidant activity compared to the fruit extract, with IC50 values of 111.81 ± 8.99µg/ml and 174.81 ± 4.86µg/ml, respectively, as determined by the DPPH scavenging assay. The nitric oxide (NO) scavenging assay also revealed higher potency in the leaves extract (IC50 = 138.59 ± 1.50µg/ml) compared to the fruit extract (IC50 = 196.47 ± 1.72µg/ml). Both extracts showed significant antimicrobial activity against all tested microorganisms. In silico studies indicated notable inhibitory activity of all phytochemicals against the target proteins, with Linoelaidic acid and 9-Octadecenamide, (Z)- exhibiting the highest activity against NADPH oxidase (PDB: 2cdu) and Shigella flexneri OspG effector kinase (PDB: 4bvu), respectively. These findings suggest that L. spinosa has potent antioxidant and antimicrobial activities. Compounds from this plant could serve as lead compounds for developing antioxidant and antibacterial agents. However, molecular studies should be addressed.

Glucose Syrup from Purple Sweet Potatoes (Ipomoea batatas L. Poir) using Acid Hydrolysis Method

&lt;p&gt;&lt;strong&gt;Abstract.&lt;/strong&gt; The making of glucose syrup from purple sweet potatoes is motivated by the high demand for alternative sugar in Indonesia. Through the processes that have been carried out and the results of previous studies, the potential of purple sweet potatoes to be processed into glucose syrup has been produced. This experiment was conducted to determine whether purple sweet potatoes can be processed into glucose syrup and to determine the suitability of the product to the standard. To convert purple sweet potatoes into glucose syrup, it is necessary to first extract the starch content in purple sweet potatoes and go through the process of making glucose syrup by the acid hydrolysis method using citric acid of various concentrations (0.2, 0.4, 0.6, 1 N). For the feasibility of consuming glucose syrup from purple sweet potatoes, a toxicity test was carried out using the Brine Shrimp Lethality Test (BSLT) method. Tests were also carried out on water, ash, reducing sugar, and starch content to then be compared with the Indonesian glucose syrup standard (SNI 01-2978-1992). The results showed that the reducing sugar content of purple sweet potato glucose syrup followed the glucose syrup quality standard, which was 43.31%, the lowest water content was 18.35%, the lowest ash content was 7.95% and there was no starch content in purple sweet potato liquid sugar. The aroma of the glucose syrup produced was following the Indonesian National Standard, but the color and taste produced were not yet in accordance. The toxicity test of purple sweet potato liquid sugar showed a lethal concentration value of LC&lt;sub&gt;50&lt;/sub&gt; &amp;gt;1,000 ppm, which means that purple sweet potato liquid sugar has no toxic potential so it is safe for consumption.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Keywords:&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;Purple Sweet Potatoes, Liquid Sugar, Acid Hydrolysis&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;br /&gt;&lt;/strong&gt;&lt;/p&gt;

Effect of Different Solvents on Toxicity and Secondary Metabolites of Mangkokan Leaves (Polyscias scutellaria (Burm.f.) Fosberg) by Brine Shrimp Lethality Test (BSLT) Method

Effect of Different Solvents on Toxicity and Secondary Metabolites of Mangkokan Leaves (Polyscias scutellaria (Burm.f.) Fosberg) by Brine Shrimp Lethality Test (BSLT) Method

Black Fruit Plant (Haplolobus Monticola) Ethanolic Extract as a Good Source of Chemical Compound Content with The Potential as A Safe Antioxidant and Antibacterial Agent

Black fruit is a native Papuan plant that faces extinction if it is not propagated. The availability of scientific information on the potential use of black fruit can help proliferate this plant. This study investigates the potential of black fruit plants in the leaves and flesh of the fruit using extraction, characterization, and extract activity testing. Extraction was carried out by maceration using a 96% ethanol solvent. Characterization was carried out by FTIR, UV-Vis spectrophotometers, and phytochemical screening. The antioxidant activity test was carried out by the DPPH method, the antibacterial test was carried out by the well diffusion method, and the toxicity test was carried out by the BSLT (Brine Shrimp Lethality Test) method. The results showed that the ethanol extract of black fruit flesh separated into two layers when stored in the refrigerator, while the leaf extract did not. Leaf extract is richer in phytochemical content compared to fruit flesh. Antioxidant and antibacterial activities were higher in leaf extracts than in fruit pulp extracts. Toxicity test results showed that the ethanol extract of black fruit leaves has the potential to be used as a food additive for antioxidant and antibacterial properties. The potential benefits of the ethanol extract of black fruit leaves and flesh can encourage cultivation and post-harvest management of this plant.

Toxicity of mangrove plant extract (Rhizophora stylosa Griff.) using the brine shrimp lethality test method

Abstract Rhizophora stylosa is a type of mangrove that is very abundant on the coast of Indonesia and contains many active compounds. So far, research on the toxicity of bark, leaf and flower extracts of R. stylosa has not been reported. This research aims to determine the toxic effects of n-hexane, dichloromethane and methanol extracts from the bark, leaves and flowers of R. stylosa through initial screening using the brine shrimp lethality test (BSLT) method and to determine the components contained in the plant. Toxicity testing using the BSLT method is widely used as the first step in extract screening because it is fast, cheap and easy to do. The results obtained were that all R. stylosa plant extracts were classified as non-toxic except for the dichloromethane extract of bark with an LC50 value of 947.76 ppm (toxic). The results of GC-MS analysis show that the compound that has the highest area is lupeol compound.

Potential bioactivity of three extracts of Lasia spinosa from freshwater lakes in Indonesia

The freshwater lake in Bogor Botanical Gardens conservation area is home to the Lasia spinosa plant, which belongs to a member of the Araceae family with a freshwater habitat. This plant is popular in Bangladesh and employed in Ayurveda as an anti-rheumatic and anti-inflammatory agent. Previous research demonstrated its antioxidant properties. This study seeks to identify toxic compounds in three types of L. spinosa extract using the Brine Shrimp Lethality Test (BSLT) using A. fransiscana shrimp larvae, so they can be developed into cytotoxic and anticancer agents. The phytochemical analysis of L. spinosa extract revealed the presence of alkaloids, phenols, flavonoids, tannins, saponins, mono- and sesquiterpenes. The LC50 values for methanol, ethyl acetate, and n-hexane extracts were 144.87 µg/mL (strong toxicity), 52.95 µg/mL (strong toxicity), and 286.94 µg/mL (weak toxicity), respectively.

Toxicity Evaluation with Brine Shrimp Lethality Test and Phytochemical Analysis of Some Indonesian Plant Extracts As Potential Anti-Colon Cancer Agents

Toxicity Evaluation with Brine Shrimp Lethality Test and Phytochemical Analysis of Some Indonesian Plant Extracts As Potential Anti-Colon Cancer Agents

Comparison of the Toxicity of Watermelon Rind Extract (Citrullus lanatus) and Melon Rind Extract Using BSLT Method

Toxicity analysis is a preliminary test to observe a toxic pharmacological activity to find a safe dose to be developed into a new drug. The plants that potentially have poisonous effects are Watermelon rind (Citrullus lanatus) and Melon rind (Cucumis melo). The purpose of this study was determine the toxic potential of watermelon rind and melon rind using Brine Shrimp Lethality Test. Test BSLT and phytochemical screening. The results of research on watermelon rind and melon rind contain secondary metabolites, amely alkaloids, flavonoids, tannins, saponins. The result of the BSLT test watermelon rind extract and melon rind extract were different. For watermelon rind extract, the LC50 value was 305,19 µg/ml and for melon rind extract, the LC50 value was 816,73 µg/ml. The LC50 value pbtained from the research results of the two samplees is toxic to Artemia salina Leach, because the LC50 value is &lt; 1000 µg/ml. Statistical test results using One Way Anova obtained a significant value of 0.027 where there were significant differences in the two samplesand various concertration, but both plants had the same toxic effect on Artemia salina Leach larvae. Keywords: Citrullus lanatus; Cucumis melo; LC50; toxicity