Stages Of Breakdown Research Articles

Right and left-sided colon cancers - specificity of molecular mechanisms in tumorigenesis and progression

BackgroundGiven the differences in embryonic origin, vascular and nervous supplies, microbiotic burden, and main physiological functions of left and right colons, tumor location is increasingly suggested to dictate tumor behavior affecting pathology, progression and prognosis. Right-sided colon cancers arise in the cecum, ascending colon, hepatic flexure and/or transverse colon, while left-sided colon cancers arise in the splenic flexure, descending, and/or sigmoid colon. In contrast to prior reports, we attempt to delineate programs of tumorigenesis independently for each side.MethodsFour hundred and eleven samples were extracted from The Cancer Genome Atlas-COAD cohort, based on a conservative sample inclusion criterion. Each side was independently analyzed with respect to their respective normal tissue, at the level of transcription, post-transcription, miRNA control and methylation in both a stage specific and stage-agnostic manner.ResultsOur results indicate a suppression of enzymes involved in various stages of carcinogen breakdown including CYP2C8, CYP4F12, GSTA1, and UGT1A within right colon tumors. This implies its reduced capacity to detoxify carcinogens, contributing to a genotoxic tumor environment, and subsequently a more aggressive phenotype. Additionally, we highlight a crucial nexus between calcium homeostasis (sensing, mobilization and absorption) and immune/GPCR signaling within left-sided tumors, possibly contributing to its reduced proliferative and metastatic potential. Interestingly, two genes SLC6A4 and HOXB13 show opposing regulatory trends within right and left tumors. Post-transcriptional regulation mediated by both RNA-binding proteins (e.g. NKRF (in left) and MSI2 (in right)) and miRNAs (e.g. miR-29a (in left); miR-155, miR181-d, miR-576 and miR23a (in right)) appear to exhibit side-specificity in control of their target transcripts and is pronounced in right colon tumors. Additionally, methylation results depict location-specific differences, with increased hypomethylation in open seas within left tumors, and increased hypermethylation of CpG islands within right tumors.ConclusionsDifferences in molecular mechanisms captured here highlight distinctions in tumorigenesis and progression between left and right colon tumors, which will serve as the basis for future studies, influencing the efficacies of existing and future diagnostic, prognostic and therapeutic interventions.

RESEARCH ON FLAVOR PRECURSORS OF MILK SERUM IN AROMATED FOAM TECHNOLOGY

The aim of this work is to study the ability of whey flavor precursors to form new volatile components under model conditions. The formation of new aromas is considered in the aspect of a two-stage process - enzymatic cleavage of the precursors and the subsequent Maillard reaction. The properties of whey components by their ability to form or modify aromatic descriptors at the stages of enzymatic cleavage of taste precursors are considered. It is proved that targeted enzymatic reactions with whey components lead to the formation of sweet, meat, cheese flavors at various stages of whey protein breakdown after interaction with carbohydrates. The influence of the depth of proteolysis on the subsequent formation of aroma from precursors is considered. It has been established that the breakdown products of milk sugar in whey do not play a significant role in the formation of aroma. Unlike proteolytic enzymes, the use of lactase (β-galactosidase) did not lead to a change in the aromatic characteristics of the modified serum. An increase in the concentration of lactulose, as a potential precursor of aroma in the sugar-amine reactions of serum, gives only a sweetish tint and does not significantly affect the change in aroma. The processes of aroma formation in modified whey have been consistently investigated, and the concept of flavored food foam based on it has been tested. It was shown that the addition of sodium chloride 5-10% to the lower residue after rectification of fermented milk whey significantly increases the stability of the aroma of the final product. It was found that the specific aroma of whey, limiting its use in food, can be modified by using new approaches to reactions with aroma precursors. We consider the fixation of the obtained aromas “soup mushroom”, “broth”, “cheese” on an oil basis for subsequent concentration and use in various products.

Gas dynamics and vorticity generation in laser-induced breakdown of air.

Research has shown that the ignition characteristics of laser-induced plasmas in fuel-air mixtures are influenced by the gas dynamics effects induced during the gas breakdown stage. Here, we present the numerical modeling of the fluid mechanics induced by breakdown (plasma formation) from a nanosecond near-infrared (NIR) laser pulse in air. The simulations focus on the post-discharge kernel dynamics with the goal of developing a better understanding of how vorticity is generated during the kernel cooling phase. Initial conditions (ICs) of kernel shape, temperature, and pressure (corresponding to the end of the laser pulse) are found from experimental Rayleigh scattering data. It is shown that this method for determining ICs is preferred versus the use of the Taylor-Sedov blast wave theory as it provides a more accurate description of the starting field. Past experimental observations have revealed that the gas dynamics of nanosecond laser sparks typically lead to the formation of an asymmetric torus with a frontal lobe propagating towards the laser source. We show that the development of the asymmetric torus is governed by strong vorticity generated through baroclinic torque arising from the blast wave that forms at the kernel boundary. Initially, the blast takes the shape of the teardrop kernel but then evolves into a spherical front during the first ∼10 µs because the blast wave strength varies along its circumference. This spatial variation leads to a misalignment between the pressure and density gradients and generation of vorticity by baroclinic torque. Ultimately, the observed flow-field is dictated by how the energy was initially deposited around the beam waist during breakdown. As such, one can tailor the aerodynamics induced during the cooling and recombination phase by controlling the energy deposition profile.

Evaluation of the Electric Field Strength in a Pre-Breakdown Ionization Wave in a Long Discharge Tube from the Emission Spectrum

The paper presents the results of studying the characteristics of a slow ionization wave (IW) arising at the initial stage of breakdown in a long discharge tube under reduced pressure. The discharge tube is a Philips TUV-30W mercury lamp with an electrode spacing of 80 cm and an inner diameter of 23 mm. The tube is filled with argon at a pressure of 2–4 Torr (nominal data) and mercury vapor. One of the electrodes is grounded, and, to the second one, positive or negative voltage pulses with an amplitude of 2 kV, a leading edge duration of ≈0.5 μs, and a repetition frequency of 1 Hz are applied. The IW velocity and the time dependence of the intensities of the Ar, Ar+, and Hg lines in the IW at different distances from the high-voltage electrode are measured. It is shown that the velocity of a positive IW (3 × 107–5 × 107 cm/s) is higher than the velocity of a negative IW (1 × 107–1.8 × 107 cm/s). The magnitude of the electric field in the front of an IW is evaluated from the comparison of the measured and calculated intensity ratios of the Ar, Ar+, and Hg lines. It is shown that, in a positive IW, the magnitude of the reduced electric field (260–450 Td) is noticeably larger than in a negative one (120–165 Td).

Density Functional Theory Study of the Initial Stages of Cl-Induced Degradation of α-Cr2O3 Passive Film

The ion exchange and point defect models are two prominent models describing the role of anions, such as chlorides, in the degradation of passive oxide films. Here the thermodynamic feasibility of critical steps of Cl-induced degradation of a hydroxylated α-Cr2O3 (0001) surface, as proposed by these two models, are studied. Both models begin with Cl substitution of surface OH and H2O, which becomes less favorable with increasing Cl coverage. The initial stages of Cl-induced breakdown of the α-Cr2O3 depend on Cl coverage and the presence of O vacancy near the surface as follows: (1) neither Cl insertion (supporting the ion exchange model) nor Cr vacancy formation (supporting the point defect model) is feasible at low Cl coverages except in the presence of O vacancies near the surface, where Cl insertion is thermodynamically feasible even at low coverages, (2) in the absence of O vacancies, Cr vacancy formation becomes feasible from 10/12 ML onwards whereas Cl insertion by exchange with subsurface OH only becomes feasible at full coverage. This implies that at higher coverages Cl-induced degradation first initiatesthrough a vacancy formation mechanism, but both insertion and vacancy formation would be feasible at full coverage.

Relaxation characteristics of ion jet formed across the short air gap in the cone–sphere electrode under positive polarity DC voltage

The research into the development of discharge under positive polarity voltage across the short air gap is of great significance to improve insulation of electrical equipment. By establishing a photoelectric joint detection system, this study observed phenomena in short air gaps before, and the period of breakdown. By utilising a photomultiplier tube and a Rogowski coil, the optical and current signals were obtained. Moreover, through z-type Schlieren system and high-speed digital camera, relaxation images of ion jet formed across cone–sphere electrodes were obtained. Through comparison and analysis, the discharge of short air gap was divided into three stages: initial corona, strong corona and streamer breakdown. On the basis of this, the relationship between optical and current signals was compared by assessing characteristics of the ion jet in each stage. Also, the relaxation characteristics of an ion jet were analysed. The results showed that ion jets appeared in the initial corona stage, while relaxation channel of the ion jet was formed and the channel fluctuated in the strong corona stage. In the streamer breakdown stage, the process of expansion, constant fluctuation and breakdown appeared; the amplitudes of corona current signals and optical signals in these three stages constantly increased.

Partial discharge development stage division based on multi-classifier fusion

The oil-paper insulation air gap discharge experiment was carried out by the step-up method, and the 29-dimensional discharge characteristic parameters were extracted. The oil-paper insulation air gap discharge experiment was carried out by the step-up method, and the 29-dimensional discharge characteristic parameters were extracted. According to K-means clustering, the partial discharge development stage is divided into four stages: initial discharge stage, discharge development stage, discharge stability stage and near breakdown stage. The three basic classifiers of random forest, support vector machine and back propagation neural network are trained by the credibility-based adaptive weighted voting algorithm to train a new fusion classifier. Compared with the traditional classification fusion device, the proposed classifier fusion model can effectively improve the accuracy of the identification of the discharge development stage, up to 87.5% or more.

Suppression of high-power microwave window breakdown by the sweeping-out-electron effect with an external dc bias electric field

To suppress microwave window breakdown, an external parallel dc bias electric field is applied to sweep out the electrons from the discharge zone. The development of microwave window breakdown under an external dc electric field is investigated using particle-in-cell and Monte Carlo collision simulation. The results show that the number of multipactor electrons decreases with the strength of the external unidirectional dc electric field when the size of the microwave window is finite. In addition, the threshold of breakdown is strongly influenced by the direction of the external bidirectional dc electric field. When the electric field is directed from the center to the edge of the microwave window, breakdown occurs more readily than in the absence of the electric field. However, when the electric field is directed toward the center of the microwave window, the discharge process starts at the edge of the window and develops to the center of the window. This is because a smaller density of electrons at the window edge results in a smaller gas pressure and the low gas pressure above the dielectric surface thus makes formation of the plasma more difficult. As a result, the early stage of breakdown is suppressed and the maximum transmitting power is enhanced.

MInscuteable regulates meiotic spindle organization during mouse oocyte meiotic maturation.

Establishment of cellular polarity is one of the key events during oocyte maturation. Inscuteable (Insc) has been identified as a key regulator of cell polarity during asymmetric division in Drosophila. However, the function of its evolutionarily conserved mammalian homologue, mInscuteable (mInsc), in mouse meiotic maturation is not clear. In this study, we investigated the roles of mInsc in mouse oocyte maturation. mInsc was detected at all stages of oocyte maturation. The protein level of mInsc was slightly higher at the germinal vesicle breakdown (GVBD) stage and remained constant during mouse oocyte maturation. The subcellular localization of mInsc overlapped with spindle microtubules. Disruption of microtubules and microfilaments caused changes in the localization of mInsc. Depletion or overexpression of mInsc significantly decreased the maturation rates of mouse oocytes. Depletion of mInsc significantly affected asymmetric division, spindle assembly, alignments of chromosomes and actin cap formation. Taken together, our results demonstrated that mInsc regulates meiotic spindle organization during mouse meiotic maturation.

Role of Prebreakdown Currents in a Static Breakdown of a Two-Sectioned Cold-Cathode Thyratron

The data on prebreakdown currents and static breakdown voltages measured in two-sectioned sealed-off thyratron with a cold cathode TPI1-10k/50 are presented. The temporal behavior of the anode voltage and of the voltage at the separate sections has been investigated at the prebreakdown and breakdown stages. It is demonstrated that the prebreakdown current presented in the separate sections causes redistribution of the anode voltage between the sections. Due to this effect, a maximum thyratron breakdown voltage can be obtained. The other method to increase the breakdown voltage is based on the forced distribution of the anode voltage between the sections using a capacitive divider. Special features of transition from the prebreakdown to breakdown current are discussed for different thyratron-switching circuits.

Monte-Carlo simulation of the ionization processes for discharges in the left branch of the Paschen curve

The paper deals with the development of the simulation methods applied to the low-pressure discharges in which the reduced electric field E/p is extremely high and there exists a problem for the correct description of the ionization processes in the classical electron avalanches. Here we use the PIC/MC code xoopic for calculation of the impact ionization coefficient α, the electron drift velocity and the electron diffusion coefficient D in the electron avalanches in nitrogen. The range of calculations covers the ratio E/p ≤ 1000 V·(cm·Torr)−1. The simulation method was tested on a simple analytical model of an electron avalanche and showed good agreement in low and moderate E/p. It is shown that in conditions of high E/p the spatial distribution of electrons in the avalanche head based on the concepts of electron mobility during their drift motion and diffusion coefficient is not quite correct. The work confirmed that at the very low pressures pre-breakdown phenomena and the transition to the next stage of high-current breakdown cannot be based on ideas about classical electron avalanches.

Multistage Metamorphic Evolution of Retrograded Eclogites from the Songshugou Complex, Qinling Orogenic Belt, China

Abstract The Qinling Orogenic Belt is one of the major collisional orogens in eastern Asia and marks the boundary between the North China Craton and South China Craton. The Songshugou complex is the largest basic to ultrabasic body to be found in the North Qinling Belt, and was emplaced as a lens-shaped body at the southern margin of the Qinling Group. A detailed petrological investigation of garnet amphibolite, augen amphibolite and well-foliated amphibolite together with garnet zoning patterns of major and trace elements, inclusions in garnet, and thermodynamic modelling indicate a multistage metamorphic history. Garnets clearly show characteristics of discontinuous growth, as they display optically light-colored snowball-textured cores surrounded by a darker mantle with few inclusions as well as chemically a sudden increase in grossular and decrease in almandine components. A partly resorbed rim is not recognized optically but mineral inclusions and a discontinuous chemical composition of garnet are proof of this third garnet growth stage. Rare earth element distribution patterns of garnet also show clear evidence for discontinuous growth and allow us to identify the reactions responsible for garnet growth. Garnet core compositions as well as amphibole inclusions allow us to constrain a P–T window where this rock equilibrated in a first stage. Calculated pseudosections and the application of the garnet–amphibole thermometer indicate an upper amphibolite- to lower granulite-facies metamorphic episode at 630–740 °C and 0·7–0·9 GPa. The presence of relict omphacite as well as a discontinuously grown garnet mantle with rutile inclusions clearly places the peak metamorphic stage in the eclogite facies. Garnet (XGrs, XAlm, XPrp) and omphacite isopleths (XMg, XNa) constrain this event at 1·7–2·1 GPa and 570–650 °C. Consistent temperatures of 500–650 °C were also determined by clinopyroxene–garnet geothermobarometry for this event. Growth of an outermost rim as well as different stages of garnet breakdown to plagioclase + amphibole coronae and the nearly complete replacement of former omphacite by a variety of symplectites point to an intricate retrograde P–T path. In more strongly retrograded samples plagioclase + amphibole ± quartz pseudomorphs entirely replace former garnet grains. Certain coronae around garnets and symplectites also contain prehnite and pumpellyite, which formed during a late retrograde stage or during a different event at very low P–T conditions (250–350 °C). Based on the detailed petrological study, we favour a multistage metamorphic history of the Songshugou metabasic rocks. The age of the eclogite-facies metamorphic event must be related to the deep subduction of the Songshugou complex during the early Paleozoic, although the age of garnet core growth remains enigmatic. The development of garnet cores indicates an upper amphibolite-facies regional metamorphic overprint succeeded by an eclogite-facies event around 500 Ma and subsequent retrogression seen in replacement of garnet and formation of symplectite. The latest imprint evidenced by prehnite and pumpellyite may be the result of fluid infiltration during the fading orogenic phase or represents a low-temperature overprint by a later process, probably related to the uplift of the North Qinling terrane at around 420 Ma.

2D particle-in-cell/Monte Carlo collisional simulation of the plasma initiation in the vacuum breakdown stage

In this paper, the plasma formation in the vacuum breakdown stage is simulated with a 2D particle-in-cell/Monte Carlo collisional model. In this model, the field is solved in a 2D cylindrical coordinate system and particles including electrons, atoms, singly, doubly, triply and fourfold charged ions are traced as super particles, each of which represents many real particles. The plasma quickly builds up from the field emission and evaporation near the field emitter with a local field of about 10 GV m−1. The plasma expands in a hemispherical shape with a high-density gradient in the vicinity of the field emitter. During the discharge, a typical structure including an ion sheath, a quasi-neutral region and an acceleration layer is obtained. The well-pronounced potential hump near the cathode, the high ion velocity in the acceleration layer and ion charge state agree well with results in previous research works. Investigation of the effects of the field enhancement factor and emitter surface temperature shows that more highly charged ions can be obtained in simulations with higher field emission current, while the plasma initiates faster and has higher density with stronger evaporation of neutral atoms.

Ontogeny of the specificity of gonadotropin receptors and gene expression in carp.

The pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), are the principle endocrine drivers of reproductive processes in the gonads of jawed vertebrates. Canonically, FSH recruits and maintains selected ovarian follicles for maturation and LH induces the stages of germinal vesicle breakdown and ovulation. In mammals, LH and FSH specifically activate cognate G-protein-coupled receptors that affect the proteins involved in steroidogenesis, protein hormone synthesis, and gametogenesis. This dual-gonadotropin model also exists in some fish species, but not in all. In fact, due to their diverse number of species, extended number of ecological niches, and remarkably flexible reproductive strategies, fish are appropriate as models to understand the co-evolution of gonadotropins and their receptors. In this study, we cloned and characterized the expression profile over the final stages of ovarian maturation of carp (Cyprinus carpio) LHCGR and FSHR. Expression of both gonadotropin receptors increased in the later stage of early vitellogenesis, suggesting that both LH and FSH play a role in the development of mature follicles. We additionally tested the activation of cLHCGR and cFSHR using homologous and heterologous recombinant gonadotropins in order to gain insight into an evolutionary model of permissive gonadotropin receptor function. These data suggest that carp (Cyprinus carpio) gonad development and maturation depends on a specific gonadotropin profile that does not reflect the temporally distinct dual-gonadotropin model observed in salmonids or mammals, and that permissive gonadotropin receptor activation is a specific feature of Ostariophysi, not all teleosts.

Investigation of Zero Crossing Detection for First Return Stroke in Negative Cloud-to-Ground Lightning Flash

This paper aims to investigate the minimum threshold for zero crossing detection of the first return stroke in negative cloud-to-ground lightning flashes. The uncertainty for a minimum threshold to be set into a programmed software for an automatic zero crossing detection motivated the author to overcome the problem. The software was built-in MATLAB. The development of the software considered the important parameter of lightning strike from the preliminary breakdown stages until the first return strokes. A single station measurement was setup in Ayer Keroh, Malacca. 41 samples of duration of the zero crossing time were recorded by using Lecoy HDO4024 with 5 MS/s. The results showed that the minimum threshold for zero crossing detection was 40µs with 82.9% accuracy. In conclusion, the minimum threshold of zero crossing detection of the first return stroke in negative cloud-to-ground was determined.

NEK5 regulates cell cycle progression during mouse oocyte maturation and preimplantation embryonic development.

NEK5, a member of never in mitosis-gene A-related protein kinase, is involved in the regulation of centrosome integrity and centrosome cohesion at mitosis in somatic cells. In this study, we investigated the expression and function of NEK5 during mouse oocyte maturation and preimplantation embryonic development. The results showed that NEK5 was expressed from germinal vesicle (GV) to metaphase II (MII) stages during oocyte maturation with the highest level of expression at the GV stage. It was shown that NEK5 localized in the cytoplasm of oocytes at GV stage, concentrated around chromosomes at germinal vesicle breakdown (GVBD) stage, and localized to the entire spindle at prometaphase I, MI and MII stages. The small interfering RNA-mediated depletion of Nek5 significantly increased the phosphorylation level of cyclin-dependent kinase 1 in oocytes, resulting in a decrease of maturation-promoting factor activity, and severely impaired GVBD. The failure of meiotic resumption caused by Nek5 depletion could be rescued by the depletion of Wee1B. We found that Nek5 depletion did not affect CDC25B translocation into the GV. We also found that NEK5 was expressed from 1-cell to blastocyst stages with the highest expression at the blastocyst stage, and Nek5 depletion severely impaired preimplantation embryonic development. This study demonstrated for the first time that NEK5 plays important roles during meiotic G2/M transition and preimplantation embryonic development.

Perinatal losses in anamnesis as a factor of fetus adaptation damage

In literature there are many works about prediction of chronic hypoxia of fetus, based on the assessment of the mother-placenta-fetus system, such as ultrasound, doppler velocimetry, cardiotocography, placental hormones and proteins tests; separate immunological, biochemical, hemostasiological and functional markers were introduced. However, the prognostic value most of these methods is insufficient by the standards of evidence-based medicine; they do not have a complex pathogenetically based approach. In this regard, the search for new methods for timely prediction and preclinical detection of fetal hypoxia continues to be a priority for obstetrics and perinatology. Cardiointervalography can be a promising method to study the relationship of the mother-placenta-fetus system; it allows simultaneous registration and assessment of the functional systems of mother and fetus. The high probability of such interrelations is evidenced by previous studies.Study aim was to assess the adaptation disorders of the fetus and newborn, to determine perinatal outcomes with a burdened obstetric history.A total of 200 pregnant women with perinatal losses in history (main group) and 100 pregnant women without perinatal losses in history (control group) were examined. Accordingto the results of fetal cardiointervalography in women with perinatal losses in history, the state of regulation of the fetal autonomic nervous system is characterized by a tendency to hypersympathicotonia, stress adaptation systems in the fetus in 66.0% of women. In 10.0% of women, hypertension of the regulatory systems of the fetus enters the stage of exhaustion and breakdown of adaptation, which is confirmed by the diagnosis of fetal distress. Assessing the condition of the fetus with an automatic analysis of the cardiotogram by the Dawes-Redman criteria showed an STV value of <4 in 89.6% of women with fetal distress. The total assessment of the biophysical profile of the fetus was 4.43 ± 0.31 points. The results of the Gausknecht test were less than 15 s, and in severe cases they dropped to 5–7 s. 76.1% of children after intrauterine distress are born in a state of asphyxia, which causes a high frequency of disadaptation syndromes: most often central nervous system disorders (73.9%) and cardiovascular disadaptation (41.3%) was occured.

HASPIN kinase mediates histone deacetylation to regulate oocyte meiotic maturation in pigs.

HASPIN kinase-catalyzed phosphorylation of histone H3 on threonine 3 (H3T3p) directs the activity and localization of chromosomal passenger complex (CPC) and spindle assembly checkpoint (SAC) to regulate chromosome condensation and segregation in both mitosis and meiosis. However, the function of HASPIN kinase in the meiotic maturation of porcine oocytes is not yet known. Here, we found that HASPIN mRNA is constantly expressed in porcine oocyte maturation and subsequent early embryo development. H3T3p is highly enriched on chromosomes at germinal vesicle breakdown (GVBD) stage and thereafter maintains a low level in progression through metaphase I (MI) to metaphase II (MII). Correspondingly, H3T3p was completely abolished in oocytes treated with an inhibitor of HASPIN kinase. Functionally, inhibition of HASPIN activity led to a significant reduction in the rate of oocyte meiotic maturation and the limited cumulus expansion. Additionally, HASPIN inhibition caused both spindle disorganization and chromosome misalignment in oocytes at MI and MII stage. Importantly, HASPIN inhibition severely prevented deacetylation of several highly conserved lysine (K) residues of histone H3 and H4 including H3K9, H3K14, H4K5, H4K8, H4K12 and H4K16 on the metaphase chromosomes during oocyte meiotic maturation. Taken together, these results demonstrate that HASPIN kinase regulates porcine oocyte meiotic maturation via modulating histone deacetylation.

Acentriolar microtubule organization centers and Ran-mediated microtubule formation pathways are both required in porcine oocytes.

Mammalian oocytes lack centrioles but can generate bipolar spindles using several different mechanisms. For example, mouse oocytes have acentriolar microtubule organization centers (MTOCs) that contain many components of the centrosome, and which initiate microtubule polymerization. On the contrary, human oocytes lack MTOCs and the Ran-mediated mechanisms may be responsible for spindle assembly. Complete knowledge of the different mechanisms of spindle assembly is lacking in various mammalian oocytes. In this study, we demonstrate that both MTOC- and Ran-mediated microtubule nucleation are required for functional meiotic metaphase I spindle generation in porcine oocytes. Acentriolar MTOC components, including Cep192 and pericentrin, were absent in the germinal vesicle and germinal vesicle breakdown stages. However, they start to colocalize to the spindle microtubules, but are absent in the meiotic spindle poles. Knockdown of Cep192 or inhibition of Polo-like kinase 1 activity impaired the recruitment of Cep192 and pericentrin to the spindles, impaired microtubule assembly, and decreased the polar body extrusion rate. When the RanGTP gradient was perturbed by the expression of dominant negative or constitutively active Ran mutants, severe defects in microtubule nucleation and cytokinesis were observed, and the localization of MTOC materials in the spindles was abolished. These results demonstrate that the stepwise involvement of MTOC- and Ran-mediated microtubule assembly is crucial for the formation of meiotic spindles in porcine oocytes, indicating the diversity of spindle formation mechanisms among mammalian oocytes.

Spatial evolution of the plasma kernel produced by nanosecond discharges in air

This work presents an experimental investigation of the hydrodynamic effects induced by nanosecond and conventional spark discharges. The energy deposited in sparks in the short breakdown time (~1 mJ mm−1) induces hydrodynamic effects that redistribute the energy over a large volume (~1 cm3) surrounding the initial plasma channel. This process influences the subsequent formation of the ignition kernel and the initiation of combustion. The experimental results presented in this paper were obtained with a set of synchronized diagnostics including schlieren, OH planar laser induced fluorescence and electrical measurements of the energy deposited in the plasma. It is shown that the motion of the gas excited after the discharge breakdown depends not only on the total deposited energy but also on the dynamics of the energy input in the plasma. Finally, the effects of nanosecond sparks are compared with those of conventional sparks used for internal combustion engines. We show that, with 20 times less energy, the nanosecond spark produces a twice bigger excited gas volume than the conventional spark. This is because the energy deposited by the nanosecond spark during the breakdown stage is three times higher than for the conventional spark.