The addition of increasing amounts of lysine to a diet of dried bread supplemented with fat, salts, and vitamins brought about significant growth responses in weanling rats. For example, a supplement of 0.5% dl-lysine·HCl, corresponding to 0.2% l-lysine, to a bread diet improved the average gain in weight after 5 weeks from 32% to about 75% of the average gain on the stock diet. An equal response is obtained from lysine added to flour before baking or from lysine added in corresponding amounts to the bread diet. At the end of 6 months the diet containing the supplement of 0.2% lysine had produced rats that were practically equal in weight to those receiving diets with larger additions of lysine. The animals on the basal bread diet did not attain weights comparable with those receiving the lysine supplements. If sufficient lysine was added to bring the total l-lysine content of the diet to about 0.8%, or more, a growth response similar to that obtained with the stock diet was observed. These results suggest that, so far as rat growth is concerned, the only important amino acid deficiency in commercial bread is lysine.
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