Application Of Brassinosteroids Research Articles

Effect of spacing and growth regulation levels on leaf area, branches and dry matter per plant of Bt. Cotton

The present study aimed to evaluate the effect of spacing and growth regulation treatments on Bt. Cotton (var. “Solar 65 BG-II) during Kharif season of 2019-20 on clayey soils of Junagadh. The experiment comprising of four levels of spacing viz., 45 cm x 30 cm, 60 cm x 30 cm, 90 cm x 30 cm, 120 cm x 45 cm and four growth regulation levels viz., control, detopping at 75 DAS, Brassinosteroid (0.15 ppm) at 75 and 90 DAS, cycocel (40 ppm) at 75 and 90 DAS were laid out in split plot design with three replications. The result showed that higher leaf area per plant at 60, 90, 120 DAS and harvest; number of sympodial branches per plant at 60, 90, 120 DAS and harvest; dry matter per plant at 60, 90, 120 DAS and harvest; CGR during 30-60 DAS; CGR during 60- 90 DAS and CGR during 90-120 DAS and CGR during 120 DAS-harvest were recorded significantly highest value with plant spacing 120 cm x 45 cm (S4). However, spacing 45 cm x 30 cm (S1) significantly increased the leaf area index per plant at 60, 90, 120 DAS and harvest. Application of cycocel (40 ppm) at 75 and 90 DAS enhanced leaf area per plant and leaf area index per plant at harvest; number of sympodial branches per plant at 90, 120 DAS and harvest; dry matter per plant at 90 DAS; CGR during 60-90 DAS; CGR during 120 DAS-harvest and RGR during 60-90 DAS. Application of brassinosteroid (0.15 ppm) at 75 and 90 DAS enhanced leaf area per plant and leaf area index per plant at 90 and 120 DAS; dry matter per plant at 120 DAS and harvest; CGR during 90-120 DAS and RGR during 90-120 DAS.

Physiological and proteomic analyses reveals that brassinosteroids application improves the chilling stress tolerance of pepper seedlings

Brassinosteroids (BRs) are important in plant resistance to chilling stress. However, limited information is available regarding the specific mechanisms involved at proteomic level. We utilized the iTRAQ proteomic approach, physiological assays and information obtained from cellular ultrastructure to clarify the underlying molecular mechanism of BRs to alleviate chilling stress in pepper (Capsicum annuum L.). Foliar application of 24-epibrassinolide (EBR) improved photosynthesis and improved cell structure by presenting a distinct mesophyll cell and chloroplast with well-developed thylakoid membranes in the leaves of pepper seedlings. We identified 346 differentially expressed proteins (DEPs), including 217 up-regulated proteins and 129 down-regulated proteins in plants under chilling (Chill) and Chill + EBR treated plants. Most of the DEPs were related to multiple pathways, including photosynthesis, carbohydrate metabolism, energy metabolism, protein biosynthesis, amino acid synthesis, redox and stress defence (ascorbate peroxidase, glutathione peroxidase and superoxide dismutase). Up-regulated DEPs were associated with the photosynthetic electron transfer chain, oxidative phosphorylation, GSH metabolism pathway, Calvin cycle and signaling pathway. The physiochemical analysis showed that EBR treatment improved the tolerance of pepper seedlings to chilling stress.

Modulation of Physiological Stress Response of Triticum aestivum L. to Glyphosate by Brassinosteroid Application.

The potential of brassinosteroids to modulate the physiological responses of winter wheat (Triticum aestivum L.) to herbicide stress was evaluated. Young winter wheat seedlings were treated with 24-epibrassinolide (EBL) and 24 h later were sprayed with glyphosate. The physiological responses of treated plants were assessed 14 days after herbicide application. Wheat growth was noticeably inhibited by glyphosate. The herbicide application significantly increased the content of the stress markers proline and malondialdehyde (MDA) evidencing oxidative damage. The content of phenolic compounds was decreased in the herbicide-treated plants. Slight activation of superoxide dismutase (SOD) and catalase (CAT) and considerable increase of glutathione reductase (GR) and guaiacol peroxidase (POX) activities were found. Increased POX and glutathione S-transferase (GST) activities were anticipated to be involved in herbicide detoxification. Conjugation with glutathione in herbicide-treated plants could explain the reduction of thiols suggesting unbalanced redox state. EBL application did not alter the plant growth but a moderate activation of antioxidant defense (POX, GR, and CAT activities and phenolic levels) and detoxifying enzyme GST was observed. The hormonal priming provoked a slight decrease in MDA and proline levels. The results demonstrate that EBL-pretreatment partly restored shoot growth and has a potential to mitigate the oxidative damages in glyphosate-treated plants through activation of the enzymatic antioxidant defense and increase of the phenolic compounds.

Production Potential of Pigeon Pea [Cajanus cajan (L.) Millsp.] as Influenced by Crop Geometry and Plant Growth Regulators

Background: Pigeonpea being highly branching and indeterminate growth habit responds very well to crop geometry. Hence to achieve potential yields, it is important to maintain optimum plant population which can effectively utilize available moisture, nutrients and solar radiation. The plant growth regulators are also known to enhance the source sink relationship and stimulate the translocation of photo assimilates, thereby increase the productivity. Methods: A field experiment was conducted at experimental farm of Agronomy Department, V.N.M.K.V., Parbhani during kharif season of 2018 and 2019. The experiment was laid out in split plot design with four main plot treatments comprised of four crop geometries as 90 cm x 20 cm, 120 cm x 20 cm 60-120 cm x 20 cm and 75-150 cm x 20 cm and sub plot treatments were five treatments on foliar application of plant growth regulators i.e. NAA @ 40 ppm, Mepiquat chloride @ 50 g a.i. ha-1, Brassinosteroids @ 0.1 ppm, Chlormequat Chloride @ 75 g a.i ha-1 and control. Result: The crop geometry of 120 cm x 20 cm and 75-150 cm x 20 cm recorded higher values of all yield parameters followed by crop geometry of 60-120 cm x 20 cm. While the seed, straw and biological yield of pigeonpea as well as highest net realization of Rs. 72072 ha-1 was obtained with crop geometry of 60-120 cm x 20 cm followed by 90 cm x 20 cm. Among the plant growth regulators foliar application of Brassinosteroids @ 0.1 ppm (G3) tended to recorded higher yield parameters, seed yield and fertility coefficient of pigeonpea as well as highest net realization followed by foliar application of NAA @ 40 ppm.

Increasing of Phenolic Compounds by Brassinosteroid Applications in Immobilized Cell Suspension Cultures of Vitis vinifera L. cv. Cinsault

In this paper, the effects on secondary metabolite accumulation of brassinosteroid (BR) (24-epibrassinolide (24-eBL) on immobilized cells that were obtained from Vitis vinifera cv. Cinsault was investigated. 24-eBL was treated to immobilized cells covered calcium alginate beads at concentrations of 0, 0.25, 0.50, 0.75 and 1.0 mg L-1 for one month. As a result of this study, it was found that 24-eBL applications modified secondary metabolite accumulation and had positive effects on secondary metabolite production when the suitable concentration was used. While the highest total phenolic, catechin, p-coumaric acid and chlorogenic acid contents were found in immobilized cells treated 0.75 mg L-1 24-eBL, the highest epicatechin, quercetin, trans-resveratrol contents were obtained in immobilized cells treated 0.50 mg L-1 24-eBL and the highest gallic acid content was determined in immobilized cells treated 0.25 mg L-1 24-eBL. Moreover, the highest 24-eBL concentration (1 mg L-1) decreased the content of secondary metabolite compared to the control (0 mg L-1 24-eBL) except total phenolic and catechin content. To conclude, 0.50 and 0.75 mg L-1 24-eBL concentrations were the most suitable concentrations for immobilized cell culture to provide the highest secondary metabolite accumulation.

Exogenous application of brassinosteroids regulates tobacco leaf size and expansion via modulation of endogenous hormones content and gene expression

The online version contains supplementary material available at 10.1007/s12298-021-00971-x.

Yield of Solanum lycopersicum l. by applying nutrients with or without fertigation interacting with an analogue brassinosteroid

Objective: To evaluate the effect of fertigation and granular fertilizer to the soil interacting with foliar application of analogue brassinosteroids in Solanum lycopersicum L. Design/Methodology/Approach: The treatments were Fertigation (F), Fertigation plus brassinosteroids (F + BR), Granular fertilization (FG) and Granular fertilization + brassinosteroids (FG + BRs). A completely randomized design with four replicates was used, where one plant from each treatment represented the experimental unit. Plants (45 days-old) were sprayed with 2 mg L-1 of Brassinosteroid CIDEF-4 and subsequent applications were made every 28 d. Plant height and stem diameter were measured 92 d after transplantation, while the yield was obtained by weighing completely red fruits. An analysis of variance was performed using the GLM procedure and the Tukey means comparison test (p?0.05) with the SAS 9.3 program. Results: Fertigation induced greater plant growth and reproduction of the tomato compared to the granular fertilizer with, or without foliar application of the analogue brassinosteroid. Treatments with granulated fertilizers decreased plant height by 10.36% and 10.45% compared to fertigation. Tomato yield with granulated fertilization, with or without Br, corresponded to 76.6% and 75.80% of what was achieved with Fertigation + Br. Study limitations/Implications: The absence of a stressful environment did not favor the application of the analogue brassinosteroid. Findings/conclusions: Fertigation induces greater growth and yield of tomato under field conditions; and environmental temperature homogeneity did not favor morphological nor physiological expression of the analogue brassinosteroid.

Hormonal response of Acutodesmus obliquus exposed to combined treatment with 24-epibrassinolide and lead

Phytohormones play key roles in many aspects of plant growth and development, as well as in resistance to abiotic stresses. The presence of heavy metal limits phytohormone endogenous level; however, the application of brassinosteroids (BRs) restores phytohormone homeostasis and reduces unfavorable consequences of heavy metal on plant growth. Therefore, the aim of this study was to (1) broaden previously published analyses/findings to study the effect of 24-epibrassinolide (EBL) and/or lead (Pb) on Acutodesmus obliquus phytohormone level after 5 days of cultivation using LC-QToF-MS quantification technique and (2) extend the total amount of quantified phytohormones in A. obliquus. The study confirmed that exogenous EBL increased the contents of gibberellin A3 (GA3), auxins (AXs) (indole-3-acetic acid, phenylacetic acid), BRs brassinolide, 24-epibrassinolide (EBL), 28-homobrassinolide, castasterone, 24-epicastasterone, typhasterol, cathasterone, 6-deoxotyphasterol, as well as different types of cytokinins (CKs): free bases, ribosides, and conjugates (N- and O-glucosides). On the other hand, treatment with Pb had an opposite effect on BRs, GA3, AXs, and free bases, as well as ribosides of CKs. The abscisic acid (ABA) level decreased under EBL treatment but increased in response to Pb stress. The level of N-glucosides, as well as O-glucosides of CKs, also significantly increased in response to Pb alone. Interestingly, the co-application of EBL and Pb led to an increase in BRs, GA3, AXs, and CKs content in the algae. Principal component analysis revealed that based on treatments, increase of GA3, BRs, and AXs was found to be in the following order: 1 μM EBL > 0.01 μM Pb + 1 μM EBL > 500 μM Pb + 1 μM EBL > control > 0.01 μM Pb > 500 μM Pb. Moreover, very strong significant linear relations between almost all studied AXs and BRs were noted. Summarizing, this research did not only allow to detect the occurrence of ABA, GA3, AXs, BRs, and CKs in A. obliquus for the first time but also to study the combined action of EBL and Pb, as well as compare it with treatment alone.

Bioregulators on seed germination and seedling growth of sweet basil

Basil (Ocimum basilicum L.) plants have multiple uses, ranging from spice to cosmetic purpose, besides being a source of essential oil and aromatic. These plants were commonly propagated by seed, however, few agrotechnologies are used to enhance the seed germination and initial growth in this crop. For this reason, our research aimed to evaluate aspects of seed germination of Ocimum basilicum L. cv. Limoncino, subjected to different bioregulators applied via substrate imbibition. The experiment was carried out in completely randomized design, with four treatments, as follows: T1: control (distilled water), T2: T13 a solution of a product formulated based on indolbutyric acid + naphthalene acetic acid + gibberellic acid (0.2 mL L-1, Liko Química), T3: gibberellic acid (0.5 mM L-1, progibb-400®) and T4: brassinosteroids (0.1 mM L-1). We evaluated: germination rate, germination speed index (GSI), first count, root and shoot length and chlorophyll a, b, total and carotenoids. The bioregulators did not influence germination, first count, GSI and photosynthetic leaf pigment, but application of gibberellic acid and brassinosteroids improve the shoot and root length, respectively. The gibberellic acid and brassinosteroids are promising bioregulators for stimulating initial basil growth.

Brassinosteroid application affects the growth and gravitropic response of maize by regulating gene expression in the roots, shoots and leaves

Brassinosteroids (BRs) are a class of plant-specific steroid hormones that play key roles in plant physiology and that actively participate in the regulation of plant responses to stress. Thus, these compounds are also considered biostimulants that could be applied to crops to improve plant performance and induce abiotic stress tolerance. In this study, with a combined physiological and molecular approach, new insights into the effects of 24-epibrassinolide (EBL), a synthetic BR, on hydroponically grown maize seedlings were gained. To this aim, a preliminary assessment of the effect of different EBL concentrations and treatment times on root elongation was evaluated to determine the concentration to use in subsequent experiments. Treatment with 1 nM EBL and an exposure time of 48 h were selected to better assess the effects of this molecule on shoot growth and the root gravitropic response. Subsequently, an untargeted RNA-Seq-based approach was applied to obtain an overview of the transcriptomic regulation occurring in the roots, shoots and leaves upon exogenous brassinosteroid application. Our outcomes highlight the substantial influence exerted by this molecule on the growth and root gravitropic response of maize seedlings. Moreover, new insights into the BR response and BR signalling in plants were gained by performing the functional characterization of differentially expressed genes via Gene Ontology (GO) and pathway analyses. Overall, this study provides useful information that could help future agricultural applications of these substances.

Effect of Brassinosteroid Application on Yield and Its Components of Wheat Genotypes Under Water Deficit Conditions

Effect of Brassinosteroid Application on Yield and Its Components of Wheat Genotypes Under Water Deficit Conditions

Study on effect of different growth hormones on growth, yield and quality of African marigold (Tagetes erecta L)

This investigations were undertaken to study the effect different growth hormones on growth and yield of African marigold (Tagetes erecta L.) under Coimbatore condition, during March 2019 to October 2019 in two different seasons. The present experiment was laid out in randomized block design with three replications involving foliar application of Gibberellic acid, Cycocel, Brassinosteroids, and silicic acid. The study revealed that foliar application of Gibberellic acid (T2) at the rate of 150ppm showed a significant improvement in morphology and yield and quality parameters in African marigold. The result recorded of maximum plant height (72.52 cm), plant spread (40.89 cm), number of primary branches (9.77), number of flowers per plant (36.52), single flower weight (6.56 g), flower diameter (6.40 cm), flower yield per plant (239.57 g) and highest total carotenoid content (1.32 mg/g) and total xanthophyll content (1.71 mg/g) and total chlorophyll content (2.60 mg/g) were recorded in the treatment T2.whereas the Cycocel (CCC) at the rate of 2000ppm recorded dry matter production (62.93 g per plant), days taken for flower bud appearance (32.61 days), days to first harvest (51.53 days) as compared to control.

Effect of Exogenous Brassinosteroid Application on Grain Yield, some Physiological Traits and Expression of Genes Related to This Hormone Signaling Pathway in Wheat under Drought Stress

Effect of Exogenous Brassinosteroid Application on Grain Yield, some Physiological Traits and Expression of Genes Related to This Hormone Signaling Pathway in Wheat under Drought Stress

Influence of drought stress and brassinosteroid on growth and Physio-biochemical characteristics of apple plants

The experiment was conducted to study the effect of drought stress and brassinosteroid on growth and physio-biochemical responses of one-year-old apple plants of ‘Super Chief’ and ‘Red Chief’ varieties. These plants were treated with foliar application of brassinosteroid 0.05 ppm and 0.10 ppm, three days prior to 15 days and 30 days of drought stress. The maximum reduction in growth parameters were registered by plants subjected to 30 days of drought stress as compared to 15 days of drought stress. The transpiration rate owed significant reduction under drought stress. Osmoregulating substances like free amino acid, total soluble sugar content and antioxidant enzyme such as catalase activity enhanced during drought stress which helped in enhancing drought tolerance. Maximum reduction of soil moisture content was registered in 30 days of drought stress over 15 days. However, brassinosteroid at both concentrations could help in maintaining vital growth and physio-biochemical processes under drought stress conditions. Foliar spray of brassinosteroid (0.05 ppm) before the imposition of stress can pave the way to minimize the deleterious effects of drought stress on apple plants.

Mitigation of the Impact of Vascular Wilt and Soil Hypoxia on Cape Gooseberry Plants by Foliar Application of Synthetic Elicitors

Physalis peruviana L. crops are exposed to different stress conditions that limit their productivity. Within these conditions, abiotic stress caused by water and biotic stress by Fusarium oxysporum f. sp. physali (Foph) are frequent at commercial levels. The foliar application of synthetic elicitors can be a tool to mitigate the negative impacts of these stresses. The objective of this study was to evaluate the interaction between Foph inoculation and three foliar applications of brassinosteroids (BR), salicylic acid (SA), and a commercial elicitor based on botanical extracts (BE) of Echinacea purpurea, Potentilla erecta, and Aloe vera on the physiological [stomatal conductance (gS), leaf water potential (Ψwf), chlorophyll fluorescence, and growth] and biochemical [photosynthetic pigments, malondialdehyde (MDA) production, and proline content] responses of cape gooseberry plants subjected to a 6-day waterlogging period. The established treatments were as follows: 1) waterlogged plants without Foph; 2) waterlogged plants with Foph; 3) waterlogged, noninoculated (Foph−) plants treated foliarly with BR, SA, or BE; and 4) waterlogged, inoculated (Foph+) plants treated foliarly with BR, SA, or BE. The results showed that the foliar application of BR or SA reduced vascular wilt development in plants subjected to a hypoxia period. In addition, three applications of BR, SA, or BE favored gS, Ψwf, growth, and chlorophyll fluorescence parameters in cape gooseberry plants under the interaction between Foph and oxygen deficit in the soil. Also, higher photosynthetic pigment and proline contents were observed in plants treated with elicitors under stress combination, whereas a lower MDA production was evidenced in this group of plants. In conclusion, BR, SA, or BE can help mitigate the negative effects of the simultaneous occurrence of Foph and a waterlogging condition for 6 days in cape gooseberry plants.

Cyclin-Dependent Kinase Inhibitors KRP1 and KRP2 Are Involved in Grain Filling and Seed Germination in Rice (Oryza sativa L.).

Cyclin-dependent kinase inhibitors known as KRPs (kip-related proteins) control the progression of plant cell cycles and modulate various plant developmental processes. However, the function of KRPs in rice remains largely unknown. In this study, two rice KRPs members, KRP1 and KRP2, were found to be predominantly expressed in developing seeds and were significantly induced by exogenous abscisic acid (ABA) and Brassinosteroid (BR) applications. Sub-cellular localization experiments showed that KRP1 was mainly localized in the nucleus of rice protoplasts. KRP1 overexpression transgenic lines (OxKRP1), krp2 single mutant (crkrp2), and krp1/krp2 double mutant (crkrp1/krp2) all exhibited significantly smaller seed width, seed length, and reduced grain weight, with impaired seed germination and retarded early seedling growth, suggesting that disturbing the normal steady state of KRP1 or KRP2 blocks seed development partly through inhibiting cell proliferation and enlargement during grain filling and seed germination. Furthermore, two cyclin-dependent protein kinases, CDKC;2 and CDKF;3, could interact with KRP1 in a yeast-two-hybrid system, indicating that KRP1 might regulate the mitosis cell cycle and endoreduplication through the two targets. In a word, this study shed novel insights into the regulatory roles of KRPs in rice seed maturation and germination.

Exogenous Auxin Induces Transverse Microtubule Arrays Through TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX Receptors.

Auxin plays a central role in controlling plant cell growth and morphogenesis. Application of auxin to light-grown seedlings elicits both axial growth and transverse patterning of the cortical microtubule cytoskeleton in hypocotyl cells. Microtubules respond to exogenous auxin within 5 min, although repatterning of the array does not initiate until 30 min after application and is complete by 2 h. To examine the requirements for auxin-induced microtubule array patterning, we used an Arabidopsis (Arabidopsis thaliana) double auxin f-box (afb) receptor mutant, afb4-8 afb5-5, that responds to conventional auxin (indole-3-acetic acid) but has a strongly diminished response to the auxin analog, picloram. We show that 5 µm picloram induces immediate changes to microtubule density and later transverse microtubule patterning in wild-type plants, but does not cause microtubule array reorganization in the afb4-8 afb5-5 mutant. Additionally, a dominant mutant (axr2-1) for the auxin coreceptor AUXIN RESPONSIVE2 (AXR2) was strongly suppressed for auxin-induced microtubule array reorganization, providing additional evidence that auxin functions through a transcriptional pathway for transverse patterning. We observed that brassinosteroid application mimicked the auxin response, showing both early and late microtubule array effects, and induced transverse patterning in the axr2-1 mutant. Application of auxin to the brassinosteroid synthesis mutant, diminuto1, induced transverse array patterning but did not produce significant axial growth. Thus, exogenous auxin induces transverse microtubule patterning through the TRANSPORT INHIBITOR 1/AUXIN F-BOX (TIR1/AFB) transcriptional pathway and can act independently of brassinosteroids.

Effect of 24-epibrassinolide on reactive oxygen species and antioxidative defense systems in tall fescue plants under lead stress

Lead is one of the most hazardous pollutants to both the environment as well as human beings. As one of the approaches to enhance phytoremediation, brassinosteroids are predicted as a potential candidate phytohormone for assisted phytoremediation. Few studies have focused on the physiological regulations of tall fescue plants (Festuca arundinacea Schreb.), a potential phytoremediation species, for its responses to applications of brassinosteroids under lead stress. Therefore, the objectives of this study were to investigate the effects of foliar application of 24-epibrassinolide, a brassinosteroids analogue, on reactive oxygen species accumulation and antioxidative defense systems of tall fescue when exposed to lead, and ultimately its potential to be used in phytoremediation. When exposed to lead (1000 mg/kg) for 80 d, decreases in shoot and root biomass of tall fescue biomass as well as chlorophyll and carotenoid productions were found. Foliar application of 24-epibrassinolide at three rates and five applications every 7 d improved the biomass of both shoots and roots, and increased the photosynthetic pigments. The improved lead tolerance in tall fescue plants after 24-epibrassinolide applications was associated with reduced H2O2 and O2.- accumulations and increased antioxidative enzyme activities including superoxide dismutase, catalase, and guaiacol peroxidase. Additionally, osmoprotectants increased and lipid peroxidation decreased. Ultimately, foliar applications of 24-epibrassinolide enhanced the lead recovery rate of tall fescue plants, proving its potential role in phytoremediation for soil contaminated with heavy metals such as lead.

Effects of Brassinosteroids on Postharvest Physiology of Horticultural Crops: A Concise Review

Brassinosteroids are natural polyhydroxylated steroidal plant growth regulators or phyto-hormones. These are ubiquitous in plant kingdom and influence a wide variety of molecular, physiological and biochemical responses of plants. Brassinosteroids have also been applied and their possible role has been investigated on postharvest physiology of various horticultural crops. Brassinosteroids regulate ripening of different non-climacteric and climacteric fruits and influence colour metabolism. They inhibit activities of peroxidase and polyphenol oxidase enzymes and delay enzymatic browning. Exogenous application of brassinosteroids inhibits cell wall degradation and delays softening of fruits. In addition, their application regulates sugar and energy metabolism in different fruit and vegetable crops. They suppress lipoxygenase and phospholipase D enzyme activities and conserve higher unsaturated fatty acid contents, suppress electrolyte leakage, inhibit lipid peroxidation and maintain higher membrane integrity eventually leading to suppressed chilling injury during postharvest storage. These alleviate oxidative stress and prolong storage life potential of various horticultural crops. So, the present review summarizes various roles and mechanism of action of brassinosteroids in extending postharvest life and maintaining quality of different horticultural crops.

Brassinosteroids application induces phosphatidic acid production and modify antioxidant enzymes activity in tobacco in calcium-dependent manner

Brassinosteroids (BRs) are steroid hormones regulating various aspects of plant metabolism, including growth, development and stress responses. However, little is known about the mechanism of their impact on antioxidant systems and phospholipid turnover. Using tobacco plants overexpressing H+/Ca2+vacuolar Arabidopsis antiporter CAX1, we showed the role of Ca2+ ion balance in the reactive oxygen species production and rapid phosphatidic acid accumulation induced by exogenous BR. Combination of our experimental results with public transcriptomic and proteomic data revealed a particular role of Ca2+-dependent phospholipid metabolizing enzymes in BR signaling. Here we provide novel insights into the role of calcium balance and lipid-derived second messengers in plant responses to exogenous BRs and propose a complex model integrating BR-mediated metabolic changes with phospholipid turnover.