Abstract
In this paper, the effects on secondary metabolite accumulation of brassinosteroid (BR) (24-epibrassinolide (24-eBL) on immobilized cells that were obtained from Vitis vinifera cv. Cinsault was investigated. 24-eBL was treated to immobilized cells covered calcium alginate beads at concentrations of 0, 0.25, 0.50, 0.75 and 1.0 mg L-1 for one month. As a result of this study, it was found that 24-eBL applications modified secondary metabolite accumulation and had positive effects on secondary metabolite production when the suitable concentration was used. While the highest total phenolic, catechin, p-coumaric acid and chlorogenic acid contents were found in immobilized cells treated 0.75 mg L-1 24-eBL, the highest epicatechin, quercetin, trans-resveratrol contents were obtained in immobilized cells treated 0.50 mg L-1 24-eBL and the highest gallic acid content was determined in immobilized cells treated 0.25 mg L-1 24-eBL. Moreover, the highest 24-eBL concentration (1 mg L-1) decreased the content of secondary metabolite compared to the control (0 mg L-1 24-eBL) except total phenolic and catechin content. To conclude, 0.50 and 0.75 mg L-1 24-eBL concentrations were the most suitable concentrations for immobilized cell culture to provide the highest secondary metabolite accumulation.
Highlights
In this paper, the effects on secondary metabolite accumulation of brassinosteroid (BR) (24-epibrassinolide (24-eBL) on immobilized cells that were obtained from Vitis vinifera cv
Phenolic compounds changed significantly according to different concentrations of 24-eBL applications (p≤0.05)
The results pointed out that the 24-eBL application caused an increase in phenolic compounds compared to control (Figure 1)
Summary
The effects on secondary metabolite accumulation of brassinosteroid (BR) (24-epibrassinolide (24-eBL) on immobilized cells that were obtained from Vitis vinifera cv. There are few studies conducted the impact of immobilization on the increased accumulation of secondary metabolites in several plants (Choi et al 1995; Gillet et al 2000; Dornenburg 2004) and grapevine (Iborra et al 1994; Guardiola et al 1996), there is no study to determine on the effect of brassinosteroid applications on phenolic contents of grapes in immobilized cultures. For this reason, more detailed studies are needed to investigate. This is the first report to our knowledge of the use of 24-eBL in immobilized cell culture in grapes
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