Relevance. Organic production is focused on maximizing the use of natural resources. Particular importance is attached to natural nitrogen fixation. Nitrogen fixation provides agricultural systems with nitrogen fixed from the atmosphere and converted into forms accessible to plants. The microbial biological “Flavobacterin”, which exhibits nitrogen-fixing activity and also suppresses a wide range of phytopathogenic fungi and bacteria, was created at the All-Russian Institute of Agricultural Microbiology.Methods. Field studies of the “Flavobacterin” effects on potatoes were carried out from 2018 to 2022 in an organic crop rotation located at the Experimental Station of the Institute for Engineering and Environmental Problems in Agricultural Production Branch of FSAC VIM.Results. The soil of the experimental plot is soddy-podzolic, light loamy gleyic on residual carbonate moraine loam, characterized by a slightly acidic reaction and a high content of organic matter. Udacha variety potatoes were cultivated. The studies were carried out with organic fertilizer — BIAGUM compost, prepared from chicken manure by industrial method. Potato tubers were treated with a “Flavobacterin” when planting, and then on the leaves. Weather conditions in summer during the years of research differed significantly from each other. In all years of the study, with the exception of the dry year 2021, the application of “Flavobacterin” significantly increased potato productivity by an average of 4.4 t/ha, without compost. In the same years, the use of compost together with “Flavobacterin” increased potato productivity by an average of 2.6 t/ha compared to options where compost was used without biological.