Bark Of Branches Research Articles

The isolation and the characterization of two polysaccharides from the branch bark of mulberry (Morus alba L.)

Two water-soluble polysaccharides termed MBBP-1 and MBBP-2 were isolated from the branches of the mulberry tree (Morus alba L.) using hot water extraction and purified on Anion-exchange DEAE52-cellulose and Sephadex G-100 column. MBBP-1 was shown to be composed of rhamnose, xylose, arabinose, mannose, glucose and galactose in the molar ratio of 4.53:2.49:4.38:4.67:17.85:5.88. MBBP-2 was composed of rhamnose, xylose, arabinose, mannose, glucose, galactose and galacturonic acid in the molar ratio of 26.85:13.8:3.14:4.4:6.1:3.19:4.9. Their structural characteristics were further investigated by FI-IR spectroscopy, Smith degradation, methylation analysis and NMR spectroscopy. Based on the data obtained, MBBP-1 had a backbone mainly consisting of (1→3)-linked glucose. MBBP-2 had a backbone mainly consisting of (1→3)-linked rhamnose and (1→2, 4)-linked xylose. Antioxidant assays indicated that antioxidant activities of MBBP-2 were significantly stronger than those of MBBP-1, and this was likely in relation to the different content of 8.2% galacturonic acid in MBBP-2.

Development of Ash Dieback in South-Eastern Germany and the Increasing Occurrence of Secondary Pathogens

Since its first identification in Poland in 2006, the ascomycete Hymenoscyphus fraxineus has caused massive dieback of Fraxinus excelsior in the countries of eastern, northern and central Europe. This work shows the development, expansion, and severity of the disease in south-eastern Germany for a period of four years, starting in 2010. Differences between habitats, as well as age classes have been captured. The presence and the amount of potentially resistant trees were proven over the years, to determine how high the resistance level might be. Typical disease symptoms are the wilting of leaves, necrotic lesions in the bark and reddish discolorations of branches and stems. In addition, stem necroses also appear by infection with species of Armillaria. Therefore, special attention has been given to Armillaria species in affected ash stands but also to other secondary pathogens, like ash bark beetles. It is shown that breeding galleries of Hylesinus fraxini are only found in trees that have recently died and thus Hylesinus fraxini is still acting as a secondary opportunistic pathogen. In contrast, Armillaria spp. can be considered as serious pathogens of weakened ash trees. In different ash stands, typical symptoms of infection can be found. A relationship between stem base necrotic lesions and vitality was examined. It is shown that necrotic lesions severely contribute to accelerating the mortality of ash trees. In addition to the high infection pressure by H. fraxineus, the high inoculum of Armillaria in the soil facilitates further infections and, thus, likewise endangers the survival of potentially resistant trees. In the following years, forest conversion and seed harvest in affected ash stands will have to be urgently considered to avoid tree gaps on a large scale. Furthermore, infection assays of potentially resistant trees with ensuing breeding programmes should be initially started for the conservation of this ecologically and economically important tree species.

Above-ground woody biomass allocation and within tree carbon and nutrient distribution of wild cherry (Prunus avium L.) – a case study

The global search for new ways to sequester carbon has already reached agricultural lands. Such land constitutes a major potential carbon sink. The production of high value timber within agroforestry systems can facilitate an in-situ carbon storage function. This is followed by a potential long term ex- situ carbon sinkwithin long lasting products such as veneer and furniture. For this purpose wild cherry (Prunus avium L.) is an interesting option for middle Europe, yielding high prices on the timber market. A total number of 39 wild cherry were sampled in 2012 and 2013 to assess the leafless above ground biomass. The complete trees including stem and branches were separated into 1 cm diameter classes. Wood and bark from sub-samples were analysed separately and nutrient content was derived. Models for biomass estimation were constructed for all tree compartments. The smallest diameter classes possess the highest proportion of bark due to smaller cross sectional area. Tree boles with a greater amount of stem wood above 10 cm in diameter will have a more constant bark proportion. Total branch bark proportion also remains relatively constant above d 1.3m measurements of 8 cm. A balance is evident between the production of new branches with a low diameter and high bark proportion offset by the thickening and a relative reduction in bark proportion in larger branches. The results show that a single tree with an age of 17 and 18 years can store up to 85 kg of carbon within the aboveground biomass portion, an amount that will increase as the tree matures. Branches display greater nutrient content than stem sections per volume unit which can be attributed to a greater bark proportion. Using the derived models the carbon and the nutrient content of above-ground woody biomass of whole trees can be calculated. Suggested values for carbon with other major and minor nutrients held within relatively immature trees strongly supports the idea of the inclusion of wild cherry within agroforestry systems as an option for carbon sequestration.

Interference effect of oral administration of mulberry branch bark powder on the incidence of type II diabetes in mice induced by streptozotocin

BackgroundDiabetes is a group of metabolic diseases that has become a global health problem worldwide. Many researchers have found that mulberry branches have a hypoglycemic effect, but there have been few studies or investigations regarding the use of mulberry branches to prevent the incidence of diabetes.ObjectiveThis study aimed to investigate the potential preventive effect of mulberry branch bark powder (MBBP) from Morus multicaulis L against type II diabetes in mice induced by streptozotocin (STZ).DesignThe normal mice were fed a diet containing 2.5, 5.0, and 10.0%, MBBP, respectively, for 2 weeks. After that, STZ (100 mg/kg) was injected into the caudal vein of these mice. These mice continued to be fed the same diet, and the fasting blood glucose (FBG) levels were monitored on the 17th and 21st days.ResultsOral administration of MBBP could effectively inhibit weight loss and maintain the FBG level. The incidence of diabetes in mice was almost inhibited by treatment with 10% MBBP. MBBP could also maintain the original antioxidant capacity and regulate the lipid metabolism in mice. An immunohistochemical assay showed that MBBP could prevent the injury of the insulin-secreting islet beta cells induced by STZ. RT-PCR also confirmed that the mRNA expression of the genes PI3K, Pdk1, Akt, and FoxO1, which were involved in the PI3K/Akt signaling pathway, hardly suffered from STZ in the 10% MBBP-dose group.ConclusionsOur results indicate that powdered mulberry branch bark has a powerful anti-diabetic effect. These results clearly illustrated that MBBP has a potential use as a health food additive in the prevention of diabetes.

Ionic liquid–lithium salt based microwave pretreatment followed by ultrasonic-assisted extraction of syringin and oleuropein from Syringa reticulata var. mandshurica branch bark by a dual response surface methodology

An ionic liquid/lithium salt solvent system was successfully introduced into the separation technique for the preparation of syringin and oleuropein fromSyringa reticulatavar.mandshuricabranch bark.

Anti-tumor effect of morusin from the branch bark of cultivated mulberry in Bel-7402 cells via the MAPK pathway

In this experiment, the morusin separated from the branch bark of cultivated mulberry, an edible medicinal plant, is used to study the inhibition of morusin in the human hepatocellular carcinoma cell line Bel-7402.

Avoidance of harvesting and sampling artefacts in hydraulic analyses: a protocol tested on Malus domestica.

A prerequisite for reliable hydraulic measurements is an accurate collection of the plant material. Thereby, the native hydraulic state of the sample has to be preserved during harvesting (i.e., cutting the plant or plant parts) and preparation (i.e., excising the target section). This is particularly difficult when harvesting has to be done under transpiring conditions. In this article, we present a harvesting and sampling protocol designed for hydraulic measurements on Malus domestica Borkh. and checked for possible sampling artefacts. To test for artefacts, we analysed the percentage loss of hydraulic conductivity, maximum specific conductivity and water contents of bark and wood of branches, taking into account conduit length, time of day of harvesting, different shoot ages and seasonal effects. Our results prove that use of appropriate protocols can avoid artefactual embolization or refilling even when the xylem is under tension at harvest. The presented protocol was developed for Malus but may also be applied for other angiosperms with similar anatomy and refilling characteristics.

Multiscale analysis of the contents of palmatine in the Nature populations of Phellodendron amurense in Northeast China

Palmatine is a valuable ingredient in Chinese medicine that is produced by Phellodendron amurense Rupr. The contents of palmatine content in root bark, trunk bark, perennial branch bark, annual branches, and leaves of the trees with different ages and geographies in Northeast China were measured by high-performance liquid chromatography. The contents of palmatine in the barks of root, trunk, and perennial branch were significantly higher than those in annual branches and leaves. The contents of palmatine in trunk bark and root bark from Lesser Khingan Mountains increased with age, which is significantly opposite to other three vegetation types. The contents of palmatine in perennial branch bark, annual bark and leaves had no significant regularity. Moreover, the contents of palmatine in samples of root bark, trunk bark, perennial branch bark and annual bark varied significantly with latitude. The nature populations of P. amurense growing at low latitude contained significantly more palmatine than those growing at high latitude. These results provide a scientific basis for the reasonable cultivation and efficient utilization of P. amurense.

Secondary metabolites of the lichen Hypogymnia physodes (L.) Nyl. and their presence in spruce (Picea abies (L.) H. Karst.) bark

Lichen species typically have a characteristic profile of secondary metabolites. Dense populations of Hypogymnia physodes growing frequently as epiphytes on tree branches have harmful effects on the host, likely due to their secondary compounds, which were undetected in tree tissues until now. The aim of the present study was to re-characterise the suite of secondary metabolites of H. physodes thalli and to estimate their translocation into spruce (Picea abies) bark. Thallus and bark extracts were compared using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The compounds were identified based on their UV, MS and MS/MS spectra as well as retention factors of their TLC analysis. In addition to the previously described secondary metabolites (protocetraric, physodalic, 3-hydroxyphysodic, physodic, and 2′-O-methylphysodic acids, atranorin and chloroatranorin) of H. physodes, further three were identified in its thalli: conphysodalic, 4-O-methylphysodic and α-alectoronic acids. Fragmentation patterns from the negative ionisation of each compound were proposed, some of which were described for the first time. Among all of the detected lichen substances, a few, e.g., physodalic, 3-hydroxyphysodic, physodic acids and atranorin, were present in the bark of spruce branches that were abundantly colonised by lichen. The newly identified compounds of H. physodes thalli may belong to its constant or accessory secondary metabolites. These compounds may be useful in the chemotaxonomic classification of this species. The presence of some lichen substances in spruce bark confirmed their ability to penetrate host tissues. These data suggest that H. physodes compounds may cause long-term effects on spruces in nature.

Study on Elephant Feeding Habit of Satkosia Tiger Reserve, Odisha, India

The Asian elephant's (Elephas maximus) feeding behavior with food preference was studied in Satkosia Tiger Reserve area between 2011 to 2014.The major objective of the present study is to document the fodder plant species consumption by elephants. Though the study area houses a good number of plant species only 110 species were identified as elephant fodder plants. The food trail of elephant was observed as branch breaking, bark peeling, twig breaking, flower plucking and stem twisting uprooting in different regions of study area during different seasons. Alteration of predominantly browsing strategy with that of grazing around the year was related to seasonal variation of food plants. Consumption of grass species (55%) was highest as compared to trees (37%), shrubs (5%), and herbs (3%). The elephants extensively fed on the plant species like Aegle marmelos, Careya arborea, Bauhinia racemosa, Kydia calycina, Bauhinia vahlii, Asparagus racemosus, Helicteres isora, Mallotus philippinensis, Madhuca indica, Zizyphus mauritiona, Mimosa pudica, , Smilax zeylanica and Diosporea species. They were fond fruits of Mangifera indica in summer. A high degree of variation in dicot- monocot ratio (47:63)) was marked during identification of elephant fodder plant by direct observation. Microscopic analysis of dung showing a high degree of variation in average dicot- monocot ratio suggested that the food plant selection of elephant was highly opportunistic and seasonal.

A new species of the genus Duolandrevus (Orthoptera: Gryllidae: Landrevinae) from China.

A bark cricket genus Duolandrevus Kirby, 1906 is discussed here. These species are brownish with depressed bodies, with reduced elytra, lack hind wings and live in the bark of dead trees or branches. Duolandrevus are distributed from the south of China and Japan to the Philippines, the Malay Archipelago and New Guinea. Only one species, Duolandrevus hongkongae Otte, 1988, was recorded in China. Here, one additional species, Duolandrevus (Eulandrevus) unguiculatus sp. nov. is described from Southern China. Its description and illustrations and the key to the subgenera of Duolandrevus worldwide are given.

The occurrence of bark beetles on cut Norway spruce branches left in managed stands relative to the foliage and bark area of the branch

This study addresses the relationships between the foliage and bark area of Norway spruce (Picea abies) branches left in the forest after managing large timber: the densities of infestation by Pityogenes chalcographus, Pityophthorus pityographus and Dryocoetes autographus; and the reproductive efficiency of P. chalcographus. Based on the models developed in this study, a positive correlation was found between the foliage area of spruce branches and the densities infested by P. chalcographus and D. autographus, though a higher negative correlation was found in relation to the size of the branch bark area (BA) and the infestation densities. A negative correlation for the branch BA was also found on P. pityographus. This result shows that the desiccation of branches affects the infestation densities of bark beetles. In contrast, the size of the branch BA was positively correlated with the reproductive efficiency of P. chalcographus, with higher reproductive efficiency on the branches from the outer layer of the pile than on disorderly scattered branches on the ground.

FIRST REPORT OF DIEBACK OF OLIVE TREES CAUSED BY NIGROSPORA sp. IN TUNISIA

During spring 2011, a new disease was observed on olive (Olea europea) cv. Chemlali in an orchard in Hencha (south east Tunisia). Inspection of the affected planting revealed reddish- brown necrotic lesions on the bark of dead twigs and branches. A fungus isolated on potato dextrose agar (PDA) from symptomatic twigs and branches had an initially white mycelium which turned gray with age. The fungus was identified as Nigrospora sp. based on morphological characteristics and analysis of the ITS gene region (White et al., 1990). A BLAST search of GenBank database revealed 100% homology with a reference sequence of Nigrospora sp. (strain P19E2, accession No. JN 207298.1). Pathogenicity tests were conducted on 10 two- year-old olive trees of cv. Chemlali by placing a mycelial plug in a shallow wound on the stem of each plant. Control plants were inoculated with sterile PDA plugs. Two months post inoculation, brown discolourations and necrotic lesions developed on inoculated stems whereas controls remained healthy. Nigrospora sp. was recovered from the necrotic lesion, fulfilling Koch's postulates.

Provenance variations in berberine content of Phellodendron amurense, a rare and endangered medicinal plant grown in Northeast China

Phellodendron amurense Rupr. produces berberine, a valuable ingredient in Chinese medicine. We examined how berberine content varied in different parts of the tree with age and geography in Northeast China. Berberine levels in root bark, trunk bark, and perennial branch bark, annual branches, and leaves were estimated by high-performance liquid chromatography. Root, trunk, and perennial branch barks had significantly higher berberine content than annual branches and leaves. Moreover, berberine content varied significantly with both longitude and latitude in samples of these three plant parts. The populations growing at low longitude and latitude contained significantly more berberine than those growing at high longitude and latitude. These results provide a scientific basis for the reasonable cultivation and efficient use of P. amurense.

Preparation of morusin from Ramulus mori and its effects on mice with transplanted H22 hepatocarcinoma.

In this study, we used branches Ramulus mori from cultivated mulberry Husang-32 (Morus multicaulis Perry) as the experimental material and anhydrous ethanol as the extraction solution to obtain the crude extract from the branch bark. The ethanolic extract was successively purified through a macroporous resin, Sephadex LH-20, and semipreparative reversed-phase high-performance liquid chromatography (RP-HPLC). The high-purity monomer was identified as morusin by HPLC with diode array detection (HPLC-DAD) and its UV spectrum. The contents of morusin exhibited almost no difference between the root and branch bark in Husang-32, and morusin was not detected in the leaves. Morusin is able to inhibit the tumor growth of transplanted H22 hepatocarcinoma in mice and has no side effects. The fluorescence quantitative real-time PCR (qRT-PCR) results indicate that morusin has a marked inhibitory effect on liver cancer cells through a mechanism that may be related increases in the expression of p53, Survivin, CyclinB1, and Caspase-3 and a decrease in NF-κ B gene expression. The influence of this compound is more apparent in the Caspase-3 and the NF-κ B genes.

Five new taraxerene-type triterpenes from the branch barks of Davidia involucrata.

Five new taraxerene-type triterpenes, 2-nor-D-friedoolean-14-en-28-ol (1), 2-nor-d-friedoolean-14-en-3α,28-diol (2), 6α-hydroxy-2-nor-D-friedoolean-14-en-3,21-dione (3), 6α,11α,29-trihydroxy-D-friedoolean-14-en-3,16,21-trione (4), and 6α,23,29-trihydroxy-D-friedoolean-14-en-3,16,21-trione (5), were isolated from the MeOH extract of the branch barks of Davidia involucrata, together with five known compounds. Their structures were elucidated by means of various spectroscopic analyses. Five of the identified compounds showed moderate cytotoxicities against the cell proliferation of SGC-7901, MCF-7, and BEL-7404.

An efficient preparation of mulberroside a from the branch bark of mulberry and its effect on the inhibition of tyrosinase activity.

A bioactive ingredient in an ethanol extract from the branch bark of cultivated mulberry Husang-32 (Morus multicaulis Perr.) was isolated using a macroporous resin column. The primary component, which was purified by semi-preparative high-performance liquid chromatography diode array detection (HPLC-DAD), was identified as mulberroside A (MA) by liquid chromatograph-mass spectrometer (LC-MS), 1H and 13C nuclear magnetic resonance (NMR) spectra. In total, 4.12 g MA was efficiently extracted from one kilogram of mulberry bark. The enzymatic analysis showed that MA inhibited the generation of dopachrome by affecting the activities of monophenolase and diphenolase of tyrosinase in vitro. This analysis indicated that MA and oxyresveratrol (OR), which is the the aglycone of mulberroside A, exhibited strong inhibition of the monophenolase activity with IC50 values of 1.29 µmol/L and 0.12 µmol/L, respectively. However, the former showed weaker inhibitory activity than the latter for diphenolase. For the monophenolase activity, the inhibitory activity of MA and OR was reversible and showed mixed type 1 inhibition. Additionally, the inhibition constant KI (the inhibition constant of the effectors on tyrosinase) values were 0.385 µmol/L and 0.926 µmol/L, respectively, and the KIS (the inhibition constants of the enzyme-substrate complex) values were 0.177 µmol/L and 0.662 µmol/L, respectively. However, MA showed competitive inhibition of diphenolase activity, and KI was 4.36 µmol/L. In contrast, OR showed noncompetitive inhibition and KI = KIS = 2.95 µmol/L. Taken together, these results provide important information concerning the inhibitory mechanism of MA on melanin synthesis, which is widely used in whitening cosmetics.

First Report of Stem Blight of Blueberry in California Caused by Neofusicoccum parvum.

In July 2013 in coastal (Santa Barbara County) California, commercial plantings of southern highbush blueberry (Vaccinium corymbosum) developed symptoms of a previously undiagnosed disease. Symptoms consisted of reddening and wilting of foliage, with leaves and small twigs later drying up. The bark of diseased branches was discolored and sunken; removal of this bark revealed a brown discoloration of the underlying wood. Approximately 5% of the planting was affected. When placed on acidified potato dextrose agar (A-PDA), surface disinfested pieces of symptomatic wood consistently yielded one type of fungus. On A-PDA, isolates produced extensive white aerial mycelium that turned dark gray after 4 to 5 days and formed pycnidia after 21 days. Three single-spore isolates were grown on PDA for 21 days for morphological and molecular characterization. Conidia were hyaline, smooth, and ellipsoid with round apices and truncated bases. Conidia measured 13 to 20 × 5 to 7.5 μm (n = 50; mean 16.7 × 6.1 μm), with a length/width ratio of 2.73. After 25 days, conidia became biseptate with a darker middle cell. rDNA sequences of the internal transcribed spacer (ITS) region of the isolates (GenBank KJ126847 to 49), amplified using primers ITS1 and ITS4 (5), were 99% identical to the holotype isolate of Neofusicoccum parvum Pennycook and Samuels (3) by a BLAST query (GU251125). Partial sequences of the translation elongation factor 1-alpha (EF1-α) gene (KJ126850 to 52), obtained using primers EF728Fa and EF986R (5), were 99% identical to N. parvum (GU251257). To demonstrate Koch's postulates, 14-day-old colonies of the three N. parvum isolates were grown on A-PDA. Using three blueberry cultivars (Abundance, Jewel, and Snowchaser), slits were cut beneath the epidermis of branches 1 cm diameter or less; one colonized agar plug (6 mm diameter) was placed into each cut and the epidermis was resealed with Parafilm. Ten inoculations (one inoculation per branch; two branches per plant) were made for each isolate and each cultivar; inoculated plants were maintained in a greenhouse. After 10 to 14 days, leaves on inoculated branches turned red and wilted, bark above and below the inoculation sites turned brown, and vascular tissue beneath the bark was also brown. After 21 days, diseased areas became sunken. N. parvum was recovered from all inoculated branches of all cultivars and matched the characteristics of the original isolates. Control branches, inoculated with sterile agar plugs, did not develop any symptoms and N. parvum was not isolated. This experiment was repeated with similar results. Many Botryosphaeriaceae species, including N. parvum, are associated with canker and dieback symptoms on blueberry worldwide (2). To our knowledge, this is the first documentation of stem blight caused by N. parvum on blueberry in CA. Blueberry is a rapidly expanding industry in the state, with 960 ha planted in 2005 increasing to 2,830 ha in 2012 (1). Drought stress predisposes plants to stem blight caused by Botryosphaeriacease species (4); therefore, expansion into arid areas of CA could increase the incidence and severity of N. parvum.

Hydrocarbons of neutral substances from the branch bark of Siberian larch

The composition of neutral substances from the bark of Larix sibirica tree verdure (branches with needles) was determined. Alkenes were found for the first time in Larix genus. The group composition of neutral substances, as well as the contents of mono-, sesqui-, di-, and triterpenes are given. The substances were identified by GLC-MS and 1H and 13C spectroscopy.

Efficacy of Erythropleum suaveolens (potrodom) and Distemonanthus benthamianus (bonsamdua) water extractives on the durability of five Ghanaian less used timber species of varying perviousness and retentiveness

Conventional wood preservatives are not only toxic to target bio-deterioration organisms but also to humans, other organisms and the environment. In an effort to find preservatives that are less toxic or non-toxic to man, other organisms and the environment, efficacy of branch bark and heartwood water extracts (0.65 g/ml) of Erythropleum suaveolens (potrodom) and Distemonanthus benthamianus (bonsamdua) were tested on five less used species (LUS) namely, Sterculia oblonga (ohaa), Antiaris toxicaria (kyenkyen), Canarium ediwonua), Celtis zenkeri (esa-kokoo) and Cola gigantea (watapuo) of varying perviousness and retentiveness from varying contents of hydrophobic and water-repellent extractives and/or extraneous substances in wood and/or vessels. Bonsandua extracts were more resistant to leaching, but potrodom extractives improved durability of Canarium schweinfurthii more than bonsamdua extractives. Potrodom bark extracts could be readily employed to preserve non-recalcitrant low durability timber species such as C. schweinfurthii, and possibly recalcitrant low durability ones such as S. oblonga after some form of pre-treatment and/or under pressure conditions. Improved durability of immersed and brushed LUS was ranked as follows: C. gigantea > C. zenkeri > S. oblonga > A. toxicaria > C. schweinfurthii proving that the natural durability of timber is somewhat dependent on their level of recalcitrance from intrinsic hydrophobic and/or water-repellent extractives and/or extraneous substances. Though extracts showed reduced efficacy with time, the use of potrodom extractives for the preservation (control of pests) of non-recalcitrant low durability wood is promising.