Due to the existence of joints, the flexural performance of segmental assembled girders is different from that of monolithic cast girders. In order to study the flexural performance of precast segmental assembled box girder with corrugated steel webs, a static failure test on a precast segmental assembled continuous box girder with corrugated steel webs and variable cross-section and a monolithic cast continuous box girder with corrugated steel webs of the same size was carried out. The influence of the existence of joints on the deflection, strain, and prestress increment of precast segmental continuous girder with corrugated steel webs was analyzed. The parameter analysis was further carried out by the finite element method. The results showed that, at the initial loading stage, the joint had little effect on the displacement, and the deflection of the segmental assembled girder changed more rapidly than that of the monolithic cast girder. In the monolithic girder, cracks mainly occurred at the middle-support position of the middle span and pure bending sections. In the segmental beam, cracks were concentrated in the concrete on both sides of the joint. The number of cracks was relatively small and concentrated in the concrete on both sides of the joints of the loading segment and the middle support segment. The trends in the strain growth in the segmental beam and MB01 were consistent, and the values were similar. The strain growth trend of the segmental girder and the monolithic girder was consistent, and the values were similar at the initial loading stage. The external prestressed tendons in the segmental girder had a greater role in the loading process than those in the monolithic girder, and the normal stress in the segmental girder increased more rapidly during the bending process. In the ultimate loading state, the external prestressing force of the segmental girder was 2.01% higher than that of the monolithic girder. The number of segments was inversely proportional to the flexural capacity of the structure. Excessive thickness of the adhesive layer would adversely affect the crack resistance of the structure. This study will contribute to the engineering application of continuous composite box girders with corrugated steel webs.