The main objective of this paper is to present the herbarium of the Pontifical Catholic University of Parana’s and its collection. The history of the HUCP had its beginning in the middle of the 1970s with the foundation of the Biology Museum that gathered both botanical and zoological specimens. In April 1979 collections were separated and the HUCP was founded with preserved specimens of algae (green, red, and brown), fungi, and embryophytes. As of October 2016, the collection encompasses nearly 25,000 specimens from 4934 species, 1609 genera, and 297 families. Most of the specimens comes from the state of Paraná but there were also specimens from many Brazilian states and other countries, mainly from South America (Chile, Argentina, Uruguay, Paraguay, and Colombia) but also from other parts of the world (Cuba, USA, Spain, Germany, China, and Australia). Our collection includes 42 fungi, 258 gymnosperms, 299 bryophytes, 2809 pteridophytes, 3158 algae, 17,832 angiosperms, and only one type of Mimosa (Mimosa tucumensis Barneby ex Ribas, M. Morales & Santos-Silva—Fabaceae). We also have botanical education and education for sustainability programs for basic and high school students and training for teachers.