The temperature is a central parameter affecting the chemical and physical properties of dense cores of interstellar clouds and their evolution to star formation. The chemistry and the dust properties are temperature dependent and the interpretation of observation requires the knowledge of the temperature and its variations. Measurement of the gas kinetic temperature is possible with molecular line spectroscopy, the ammonia molecule, NH3, being the most commonly used tracer. We want to determine the accuracy of the temperature estimates derived from ammonia spectra. The normal interpretation of NH3 observations assumes that all the hyperfine line components are tracing the same gas volume. In the case of temperature gradients they may be sensitive to different layers and cause errors in the optical depth and gas temperature estimates. We examine a series of spherical cloud models, 1.0 and 0.5 M_Sun Bonnor-Ebert spheres, with different radial temperature profiles. We calculate synthetic NH3 spectra and compare the derived column densities and temperatures to the true values. For high signal-to-noise observations, the estimated gas kinetic temperatures are within ~0.3 K of the real mass averaged temperature and the column densities are correct to within ~10%. When the S/N ratio of the (2,2) spectrum decreases below 10, the temperature errors are of the order of 1K but without a significant bias. When the density of the models is increased by a factor of a few, the results begin to show significant bias because of the saturation of the (1,1) main group. The ammonia spectra are found to be a reliable tracer of the mass averaged gas temperature. Because the radial temperature profiles of the cores are not well constrained, the central temperature could still differ from this value. If the cores are optically very thick, there are no guarantees of the accuracy.
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