Bone Structure Research Articles

PRESERVATION OF DISTAL BONE DEFECT AFTER SURGICAL EXTRACTION OF IMPACTED THIRD MOLAR

Extracted teeth are still considered a clinical waste and therefore being discarded. It is evident that obtained and prepared autogenous dentin graft (ADG) may be used for guided bone regeneration (GBR) because of similar biochemical characteristics to human bone. Aim: To present a novel procedure in a clinical setting that employs freshly extracted teeth that are processed into a bacteria-free particulate dentin, and then grafted immediately into extraction sites or bone deffects. Monitoring the clinical and radiological parameters in the postextraction defects of the alveolar ridges for a period of 6 months, proving the rapid healing capacity of ADG on the bonе and soft tissue structures in the jaw bones. Methods: Clinical measurements were performed using a questionnaire for monitoring the postoperative clinical manifestation, bone measuring calipers and for measuring the horizontal changes of the alveolar ridge and a graduated probe for measuring vertical dimensional changes, also paraclinical-radiological examinations to follow-up bone density. Results: During the follow period of six months, clinical measurements of post-extraction dimensional changes of the alveolar ridges shows minimal horizontal and vertical bone resorption with preserved alveolar ridge volume, with an accelerated bone regenerative process without special postoperative complications. Conclusion: Dentin particulate grafted immediately after extractions should be considered as the gold standard due to its osteogenetic, osteoinductive and osteoconductive effects on bone tissue regeneration.

Bone fragility in young people: significant anamnestic elements

Various factors are known to interfere with gain in bone density and structure during growth and development. Bone status in childhood has been shown to be a predictor of bone mass in young adulthood. This concise review aims to discuss the main factors that can influence peak bone mass during growth and development, and whether they may be predictors of future bone fragility risk, useful for physicians taking care of children and adolescents. KEY WORDS: Children and adolescent bone health, peak bone mass, predictors of bone fragility, osteoporosis prevention.

Spatiotemporal Characterization of Microstructure Morphology, Mechanical Properties and Bone Remodeling of Rat Tibia Under Uniaxial Compressive Overload Loading.

Bone tissue is subjected to increased mechanical stress during high-intensity work. Inadequate bone remodeling reparability can result in the continuous accumulation of microdamage, leading to stress fractures. The aim of this work was to investigate the characteristics and repair mechanisms of tibial microdamage under several degrees of overload. Also, we aimed at better understanding the effects of overload on the multi-scale structure and mechanical properties of bone. Sixty 5-month female rats were divided into three groups with different time points. Micro-CT was used to evaluate the three-dimensional microstructure, and three-point bending, quasi-static fracture toughness and creep mechanical test were carried out to evaluate the mechanical properties. SEM was used to observe the morphological characteristics of fracture surfaces. Section staining was used to count the microdamage parameters and numbers of osteoblasts and osteoclasts. The microarchitectures of cancellous and cortical bones in the three overload groups showed different degrees of damage. Overload led to a messy crystal structure of cortical bone, with slender microcracks mixed in, and a large number of broken fibers of cancellous bone. The properties associated with the elastic plasticity, fracture toughness, and viscoelasticity of cortical bone reduced in three groups, with that corresponding to day 30 presenting the highest damage. The accumulation of microdamage mainly occurred in the first 14days, that is, the crack density peaked on day 14. Peak-targeted bone remodeling of cortical and cancellous bones occurred mainly between days 14 and 30. The influence of overload mechanical environment on bone quality at different time points was deeply investigated, which is of great significance for the etiology and treatment of stress fractures.

A Review of Radiology and Optical Coherence Tomography in Dental Diagnostics

Background: The rapid evolution of imaging technologies has significantly transformed dentistry, improving the diagnosis and treatment of various dental conditions. While traditional radiological methods such as X-rays and Cone Beam Computed Tomography (CBCT) remain the cornerstone of dental imaging, the emergence of Optical Coherence Tomography (OCT) offers a non-invasive, radiation-free alternative. This review examines the comparative efficacy of X-ray and OCT technologies in diagnosing hard tissue conditions in the oral cavity. Methods: The review analyzed the application of OCT and radiology in dental diagnostics through a custom-built Swept-Source OCT system, assessing its performance against traditional radiological methods. The study utilized both in vivo and ex vivo evaluations, considering variables such as resolution, depth penetration, and image clarity across different dental conditions. Results: OCT demonstrated superior capabilities in detecting early-stage cavities, fractures, and minor imperfections, particularly when high-resolution imaging was required. However, its limited penetration compared to X-rays restricted its application in comprehensive bone assessments. Radiology, especially CBCT, provided essential volumetric data crucial for evaluating bone density, fractures, and post-surgical conditions, though it posed concerns related to radiation exposure. Combining both modalities proved beneficial, offering complementary insights that enhanced overall diagnostic accuracy. Conclusion: While X-ray-based radiology remains indispensable for broad evaluations of dental and bone structures, OCT presents a valuable adjunct, particularly for high-precision assessments of soft tissues and small surface defects. The synergistic use of these technologies can provide a more holistic approach to dental diagnostics, potentially improving patient care and treatment outcomes. Future advancements in OCT could further expand its role in routine dental practice, enhancing diagnostic accuracy while minimizing radiation exposure risks.

A Comprehensive Review on Understanding Magnesium Disorders: Pathophysiology, Clinical Manifestations, and Management Strategies.

Magnesium is vital in a broad spectrum of physiological processes, including enzyme activity, energy production, and neuromuscular function. Despite its crucial role, magnesium disorders - comprising both deficiency (hypomagnesemia) and excess (hypermagnesemia) - are frequently underrecognized and inadequately managed in clinical practice. Magnesium deficiency is widespread, particularly among populations with chronic illnesses, the elderly, and those experiencing malnutrition, often leading to significant neuromuscular, cardiovascular, and metabolic complications. Conversely, hypermagnesemia, though less common, poses serious risks, especially in individuals with impaired renal function or those receiving high doses of magnesium supplements or medications. This review comprehensively examines magnesium disorders, detailing their pathophysiology, clinical manifestations, and management strategies. It highlights the essential functions of magnesium in maintaining cellular integrity, cardiovascular health, and bone structure and discusses the global prevalence and risk factors associated with magnesium imbalances. By offering insights into the current understanding of magnesium homeostasis and its disruptions, this review aims to enhance the awareness and treatment of magnesium-related conditions, ultimately improving patient outcomes across diverse clinical settings.

The markers of the predictive DNA test for canine hip dysplasia may have a stronger relationship with elbow dysplasia

Canine hip and elbow dysplasias, which are prevalent orthopedic conditions rooted in developmental and hereditary factors are yet to be comprehensively assessed. This study aimed to address this gap by exploring the prognostic significance of five markers linked to canine hip dysplasia using available genome-wide association studies (GWAS) data. The influence of these markers on both hip and elbow dysplasia was examined in dogs exposed to standardized environmental conditions. We made a groundbreaking discovery using custom primers, qPCR assays, and evaluation of fluorescent resonance energy transfer (FRET) probes. Three specific SNPs previously associated with the risk of canine hip dysplasia demonstrated a potentially stronger correlation with elbow dysplasia. Notably, the SNP at nucleotide position 22691322, located near the canine CHST3 gene, displayed significance as a marker in multivariable logistic regression analysis. Surprisingly, none of the initially targeted SNPs showed a direct association with hip dysplasia. The genomic positions of these SNPs reside within a region conserved across mammals. In silico analyses suggested that the relevant variant might be positioned in a region linked to bone and muscle structures. Our findings revealed a remarkable relationship between SNP2 genotypes and methylation patterns, shedding light on the underlying mechanism that partially explains the genotype-phenotype correlation in canine CHST3. These groundbreaking findings offer essential insights for future, more extensive investigations into canine orthopedic health. This research significantly contributes to our understanding of the molecular foundations of hip and elbow dysplasia in dogs by charting a course for advancements in veterinary medicine and the overall well-being of canine companions.

Sound speed imaging of cortical bone and soft tissue based on frequency-domain ultrasound waveform tomography

Abstract As one of the ultrasound computed tomography (UCT) algorithms, full-waveform inversion (FWI) can recover clear and detailed internal structures of bones by iteratively minimizing the misfit between observed and numerical data. However, time domain full-waveform inversion (TDFWI) often lacks sufficient low-frequency information, and the computation of full-time data imposes a significant computational burden. Besides, there are few studies on whole-medium reconstruction that jointly invert the bone and surrounding soft tissues. In this study, an FWI-based frequency-domain UCT (FD-UCT) is utilized for simultaneously imaging the sound speed of bones and soft tissues, which facilitates the inversion efficiency while ensuring the existence of low-frequency information. Simulated studies show that the FD-UCT can both reconstruct bones with large acoustic impedance and distinguish subtle impedance differences within soft tissues to reveal lesions.

Evaluation of Tibiofemoral Contact Mechanics After a Novel Hybrid Procedure for Femoral Osteochondral Defect Repairs With a Subchondral Implant and Dermal Matrix.

There is a lack of procedures that adequately address the subchondral bone structure and function for reconstructing osteochondral defects in the femoral condyles. To biomechanically evaluate the tibiofemoral joint contact characteristics before and after reconstruction of femoral condylar osteochondral defects using a novel hybrid reconstructive procedure, which was hypothesized to restore the contact characteristics to the intact condition. Controlled laboratory study. Tibiofemoral contact areas, contact forces, and mean contact pressures were measured in 8 cadaveric knees (mean age 52 ± 11 years; 6 women, 2 men) using a custom testing system and pressure mapping sensors. Five conditions were tested for each condyle: intact, 8-mm defect, 8-mm repair, 10-mm defect, and 10-mm repair. Medial femoral condylar defects were evaluated at 30° of knee flexion and lateral condylar defects were evaluated at 60° of knee flexion, with compressive loads of 50, 100, and 150 N. The defects were reconstructed with a titanium fenestrated threaded implant countersunk in the subchondral bone and an acellular dermal matrix allograft. Repeated-measures analysis of variance with Bonferroni correction for multiple comparisons was used to compare the results between the 5 testing conditions at each load. Medial condylar defects significantly increased mean contact pressure on the lateral side (P < .042), which was restored to the intact levels with repair. The lateral condylar defect decreased the mean contact pressure laterally while increasing the mean pressure medially. The lateral and medial mean contact pressures were restored to intact levels with the 8-mm lateral condylar defect repair. The medial mean contact pressure was restored to intact levels with the 10-mm lateral condylar defect repair. The lateral mean contact pressure decreased compared with the intact state with the lateral condylar 10-mm defect repair. Tibiofemoral joint contact pressure was restored to the intact condition after reconstruction of osteochondral defects with dermal allograft matrix and subchondral implants for the repair of both 8- and 10-mm lateral condylar defects as well as 8-mm medial condylar defects but not completely for 10-mm medial condylar defects. The novel hybrid procedure for osteochondral defect repair restored tibiofemoral joint contact characteristics to normal in a cadaveric model.

Sex-specific trisomic Dyrk1a-related skeletal phenotypes during development in a Down syndrome model.

Skeletal insufficiency affects all individuals with Down syndrome (DS) or trisomy 21 and may alter bone strength throughout development due to a reduced period of bone formation and early attainment of peak bone mass compared to those in typically developing individuals. Appendicular skeletal deficits also appear in males before females with DS. In femurs of male Ts65Dn DS model mice, cortical deficits were pronounced throughout development, but trabecular deficits and Dyrk1a overexpression were transitory until postnatal day (P) 30, when there were persistent trabecular and cortical deficits and Dyrk1a was trending toward overexpression. Correction of DS-related skeletal deficits by a purported DYRK1A inhibitor or through genetic means beginning at P21 was not effective at P30, but germline normalization of Dyrk1a improved male bone structure by P36. Trabecular and cortical deficits in female Ts65Dn mice were evident at P30 but subsided by P36, typifying periodic developmental skeletal normalizations that progressed to more prominent bone deficiencies. Sex-dependent differences in skeletal deficits with a delayed impact of trisomic Dyrk1a are important to find temporally specific treatment periods for bone and other phenotypes associated with trisomy 21.

Fracture Non-Union in Osteoporotic Bones: Current Practice and Future Directions.

Given the compromised bone quality and altered healing environment, fracture non-union in osteoporotic bones presents a complex challenge in orthopedics. As global populations age, the incidence of osteoporotic fractures rises, leading to increased delayed healing and non-union cases. The pathophysiology underlying non-union in osteoporotic patients involves impaired bone regeneration, reduced osteoblast function, and poor vascularity. Traditional management strategies - ranging from pharmacological interventions like bisphosphonates and teriparatides to surgical approaches such as bone grafting and mechanical fixation - often yield limited success due to the weakened bone structure. Recent advances, however, have introduced novel therapies such as growth factors, stem cell applications, gene therapy, and bioactive scaffolds that offer more targeted and biologically driven solutions. Emerging technologies like three-dimensional printing and nanotechnology further contribute to customized treatment strategies that hold promise for improved outcomes. Diagnostic approaches have also evolved, integrating radiological assessments and biomarkers to identify patients at risk for non-union better. Despite these advancements, challenges remain, including the high costs, technical complexities, and the need for more robust clinical evidence. Future directions involve optimizing these innovative treatments, validating their effectiveness in more extensive clinical trials, and integrating personalized medicine approaches to cater to the individual needs of osteoporotic patients. Overall, integrating these emerging therapeutic strategies alongside traditional practices represents a significant shift towards more effective and personalized management of fracture non-union in osteoporotic bones.

A numerical simulation method for ultrasonic transmission in cancellous bone based on a four-parameter random growth method

Abstract Quantitative ultrasound (QUS) has been widely used in non-destructive evaluation of bone health in research and clinical practice. To make a more accurate bone evaluation, the transmission characteristics of ultrasound in the bone need to be understood in detail. In the two-dimensional finite element model, cancellous bone is usually simulated by a non-porous structure solid or by approximating bone trabeculae as ellipses, which is different from real bone. However, although the error of the model constructed by bone CT images is small, it needs to be based on real bone samples, and the samples are limited. Therefore, a modeling method of cancellous bone based on four-parameter random growth method was proposed in this paper, and on this basis, numerical simulation of ultrasonic transmission was carried out. Firstly, based on the four-parameter random growth method, the aggregation algorithm is used to concentrate discrete pixels and smooth the edge of pores. Meanwhile, the built-in algorithm ensures the same porosity before and after processing to reduce the discrete pore structure. Secondly, based on COMSOL to establish the simulation model of ultrasonic propagation in cancellous bone, we analyzed the change of acoustic field distribution, discussed the correlation between the porosity of cancellous bone and backscattering coefficient (BSC) based on the ultrasonic backscattering method, and compared the experimental results of CT scan images of bone samples. The experimental results show that the cancellous bone modeling method in this paper has the same conclusion as the method based on CT images, which verifies the feasibility of this method. This method can generate a geometric model of the cancellous bone microstructure with specified porosity and different bone trabecular distribution, which is similar to the real bone structure, and can be directly imported into the finite element software to facilitate the study of bone microstructure related problems. It provides a more convenient method to study the mechanism of ultrasonic propagation in cancellous bone and has important significance in solving the inverse problem of recovering effective bone parameters from the received ultrasonic signals.

Causal relationships between height and weight with distal tibia microarchitecture and geometry in adult female twin pairs.

Higher stature and lower weight are associated with increased risk of fracture. However, the pathophysiology for the associations of height and weight with bone microarchitecture and geometry is unclear. We examined whether these associations were consistent with causation and/or with shared familial factors. In this cross-sectional study of 566 female twins aged 26-76yr, a regression analysis for twin data, Inference about Causation by Examination of FAmilial CONfounding (ICE FALCON), was used for testing causation. The bone microarchitecture and geometry of the distal tibia was assessed using HR-pQCT and the StrAx1.0 software. Higher stature was associated with larger total bone cross-sectional area (CSA), lower total bone volumetric bone mineral density (vBMD), larger cortical CSA, thinner cortices, higher porosity of the total cortex, compact cortex, outer and inner transitional zone (TZ), lower cortical vBMD, and larger medullary CSA (regression coefficients (β) ranging from -.37 to .60, all p<.05). Using ICE FALCON, the cross-pair cross-trait associations attenuated toward zero after adjusting for the within-individual association (absolute values of β ranging from .05 to .31, all p<.001). Higher weight was associated with higher total bone vBMD, larger cortical CSA and thicker cortices, lower porosity of the total cortex and inner TZ, and higher cortical vBMD (β ranging from -.23 to .34, all p<.001), and thinner trabeculae, higher trabecular number, lower trabecular separation, and higher trabecular vBMD (β ranging from -.31 to .39, all p<.05). Only cortical CSA attenuated toward zero after adjusting for the within-individual association between weight and bone microarchitecture (β = .042, p=.046). Higher stature was associated with a weaker cortical, not trabecular bone traits, whereas higher weight was associated with stronger cortical and trabecular bone traits. The results were consistent with height having a causal effect on weaker cortical bone structure, whereas weight had a casual effect on the larger cortical CSA.

Impact of Hydroxyapatite Nanoparticles, Demineralized and Decellularized Bone Matrix on Tibia Fracture Healing: A Biochemical and Histological Study in Goats

At present, using bone grafts is the most successful surgical approach for repairing deficits in the bone structure. This study investigated into how goat tibial defect fracture healing was impacted by using hydroxyapatite (HA) or demineralized and decellularized bone matrix (dDBM) xenograft. Methodology: In this study, fifteen young goats were employed. Three groups (n=5) consisting of the control group, nano-group, and xeno-group were created from the animals. Bone drill was used to remove 2 mm from the tibia of the right hind limp in all animals. The hydroxyapatite nanoparticles (HA) in the Nano-group and the xenograft demineralized and decellularized bone matrix (dDBM) (obtained from sheep tibia) in the Xeno-group were used to fill the bone defect respectively, while the bone defect in the control group remained without treatment, and the surgical site was regularly closed. Blood serum was collected at the following intervals: postoperative, 14, 42, and 64 days for detection of alkaline phosphatase (ALP) and osteocalcin (OC) levels. At 64 days after surgery, all goats were euthanized and the healing was evaluated by specimen histological observation. Results: Biochemical examination in treated groups (Nano and Xeno groups) showed a statistically significant (P &lt; 0.05) increase in the level of ALP and OC. histopathological examination at 64 day post-operation, treated groups shows Completely grown woven bone and the fracture callus composed of mature fibro-osteoid mixture. In conclusion, We demonstrate that the healing process accelerated and the bone defect can be filled by using hydroxyapatite (HA), or dDBM, as a scaffold for the damaged tibia bone.

Relationship between interincisal angles and TMJ morphology/position and trabecular structure: a retrospective study

BackgroundTMJ morphology/position and trabecular structure are influenced by various factors. The role of the interincisal angle, an indicator of the anterior occlusal relationship, on TMJ remains unclear. This study aims to investigate the morphology, trabecular bone structure, and position of the condyle, as well as the glenoid fossa’s morphology in skeletal class II populations with different interincisal angles.Materials and methodsA total of 150 adult patients with normodivergent facial types and skeletal class II malocclusions were selected and divided into three groups based on their interincisal angles: normal, small, and large angle groups. The indexes of TMJ were measured using cone-beam computed tomography (CBCT) data and analyzed using Dolphin Imaging, Mimics, and ImageJ.ResultsThe small angle group had the smallest anteroposterior diameter (APD), while the large angle group had a greater mediolateral diameter (MLD). The large angle group exhibited significantly the largest maximum axial area, bone surface area, and bone volume (P < 0.05). Small and large angle groups exhibited greater bone trabeculae (Tb. N) and thinner trabecular thickness (Tb. Th). Compared to the normal angle group, the small angle group exhibited a larger horizontal condylar angle and smaller bilateral condylar angles on the axial plane, while the large angle group showed the opposite trend. Small and large angle groups showed a reduced vertical condyle angle on the coronal plane, with the largest reduction observed in the large angle group (P < 0.05). Small and large angle groups had higher heights of the glenoid fossa (GFH). The large angle group exhibited the greatest GFH and width of the glenoid fossa (GFW) (P < 0.05).ConclusionThe large angle group had elongated oval and large condyles, and deeper glenoid fossae, while a flattened-oval and smaller condyle, and wider and shallower glenoid fossae were observed in the small angle group. Small and large interincisal angle affects the structure of condylar trabeculae, resulting in thinner Tb. Th and greater Th. N. In the condylar position, small and large angle groups exhibit condylar rotation in the axial and coronal planes. Therefore, the interincisal angle affects the morphology, position, and trabecular structure of the TMJ. This implies that we must pay attention to the impact of the interincisal angle on TMJ, and it is crucial to restore the normal interincisal angle during orthodontic treatment.

ГИБРИДНОЕ ОРТОДОНТИЧЕСКОЕ ЛЕЧЕНИЕ С ПРИМЕНЕНИЕМ ТРИЛОН-Б ЭЛЕКТРОФОРЕЗА. КЛИНИЧЕСКИЙ СЛУЧАЙ

The purpose of the study is to optimize the treatment of patients with dentofacial anomalies and deformities using complex orthodontic treatment using Trilon-B electrophoresis. The article presents a clinical example of hybrid orthodontic treatment using Trilon-B electrophoresis of a patient aged 37 years with a diagnosis of “distal occlusion, skeletal class 2”. Materials and methods. A comprehensive diagnosis of the condition of the patient’s dental system was carried out: clinical examination; photometry; anthropometry of dentition on models obtained using 3D scanning; X-ray examination methods (OPTG, CBCT); measurement of bone tissue density before treatment in the Picasso Viewer program. The next stage was making a diagnosis and drawing up a treatment plan, consisting of pre-orthodontic preparation of bone structures using Trilon-B electrophoresis and direct orthodontic treatment. Results. After the combined treatment, an optimal functional and aesthetic result was achieved. The proposed method of orthodontic treatment using Trilon-B electrophoresis made it possible to shorten the active period of treatment and reduce the risk of possible complications due to short-term wearing of orthodontic equipment, and was also successfully used in the complex treatment of 23 patients with dentoalveolar anomalies and deformities.

Paracellular Transport and Renal Tubule Calcium Handling: Emerging Roles in Kidney Stone Disease.

The kidney plays a major role in maintenance of serum calcium concentration, which must be kept within a narrow range to avoid disruption of numerous physiologic processes that depend critically on the level of extracellular calcium, including cell signaling, bone structure, and muscle and nerve function. This defense of systemic calcium homeostasis comes, however, at the expense of the dumping of calcium into the kidney tissue and urine. Because of the large size and multivalency of the calcium ion, its salts are the least soluble among all the major cations in the body. The potential pathologic consequences of this are nephrocalcinosis and kidney stone disease. In this review, we discuss recent advances that have highlighted critical roles for the proximal tubule and thick ascending limb in renal calcium reabsorption, elucidated the molecular mechanisms for paracellular transport in these segments, and implicated disturbances in these processes in human disease.

Charcot reconstruction using a novel intramedullary nail along the medial column: 3-5 year follow-up results

Charcot neuroarthropathy is a destructive, chronic disease of the bone structure and joints, and is identified in patients experiencing peripheral neuropathy, frequently associated with diabetes. The foot and ankle are most impacted by Charcot neuroarthropathy; whereas the goal of management is to stabilize the joint to avoid pedal collapse, infection, and foot amputation. Novel surgical approaches and hardware have shown promise in achieving these goals, allowing patients to return to ambulation, while avoiding late-stage complications. This multicenter, retrospective, consecutive case series reviewed individuals with midfoot Charcot neuroarthropathy who underwent surgical reconstruction using a novel locking intramedullary nail (LIN) in the medial column with at least 36 months of follow-up. Patients were treated between 2019 and 2021.This report presents data and outcomes on 30 patients with Charcot disease treated with a LIN to achieve stability and fusion along the medial column of the reconstruction, with 36-60 months of follow-up data. Of these patients, 93.3% (n = 28) demonstrated complete (multiple joint) fusion along the medial column while there were zero nonunions. Overall, the mean time to fusion was 14.1 weeks (range 10-18 weeks) and 18.1 weeks mean time to functional braced-weightbearing (range 12-28 weeks). Overall, this retrospective review investigated the long-term use of a novel LIN in Charcot neuroarthropathy patients. The success of this LIN was demonstrated by the rate of fusion and time to functional braced-weightbearing, and safety was demonstrated by the low incidence of adverse events. Overall, these results indicate that use of a LIN within the medial column to be successful in the surgical reconstruction of an unstable midfoot Charcot deformity.

Crossing length scales: X-ray approaches to studying the structure of biological materials.

Biological materials have outstanding properties. With ease, challenging mechanical, optical or electrical properties are realised from comparatively `humble' building blocks. The key strategy to realise these properties is through extensive hierarchical structuring of the material from the millimetre to the nanometre scale in 3D. Though hierarchical structuring in biological materials has long been recognized, the 3D characterization of such structures remains a challenge. To understand the behaviour of materials, multimodal and multi-scale characterization approaches are needed. In this review, we outline current X-ray analysis approaches using the structures of bone and shells as examples. We show how recent advances have aided our understanding of hierarchical structures and their functions, and how these could be exploited for future research directions. We also discuss current roadblocks including radiation damage, data quantity and sample preparation, as well as strategies to address them.

From computed tomography to 3D printed heterogeneous scaffolds: A novel approach to simulating bone characteristics using octree adaptive subdivision

Bone microstructures or scaffolds mimicking natural bone characteristics find widespread applications in bone tissue engineering (BTE). To accurately replicate a personalized bone structure, we developed a heterogeneous scaffold by combining medical image data with the octree adaptive subdivision technique. The transformations between Hounsfield units (HU)-strength and strength-lattice features were implemented for mapping bone information to the scaffold. Initially, HU values from computed tomography (CT) images were smoothly converted into corresponding strengths through a rational Bezier curve. Then, these strengths were further translated into the features of lattice structures, specifically the strut diameter and subdivision level. The novel concept of adaptive octree subdivision, executed on the lattices, allows for controlling bone porosity and mechanical property even at local levels. As a result, a personalized heterogeneous scaffold accurately simulating natural bone structure was designed and manufactured for bone grafting application on a 3D-printed alveolar model. The scaffold has an outer cortical bone layer capable of withstanding pressures of up to 208.2 MPa, while the inner cancellous bone exhibits a high porosity ranging from 77.6 % to 93.6 %. The research provides an elegant yet effective approach to the generation of bone microstructures, opening up practical BTE applications.

Modeling and verification of cortical bone drilling forces based on tissue structure heterogeneity

Bone drilling mechanism study is the basis for the optimization of cortical bone drilling process and drill geometry. In this paper, a drilling force model for modified drills with thinned chisel edge is established considering the heterogeneous structure of cortical bone. The bone mineral density is embedded into the established model, the model can predict the axial thrust force change along the drilling depth direction, and the model is verified through cortical bone drilling experiments. The thrust force and torque predicted by the model are in good agreement with the drilling experimental results of cortical bone drilling. Then, the drilling performance of the modified drill and the common drill is compared through cortical bone drilling experiments. Compared with common drill bits, the maximum reduction in average thrust force of the modified drill bit during stable drilling is 21.21 % (n = 2500 rpm, Vf=60 mm/min). The maximum reduction in average roughness of the hole wall is 21.87 % (n = 500 rpm, Vf=10 mm/min). The drill chisel edge thinning design reduces the negative impact of the negative normal rake angle on the cutting lip of common drill on drilling force and stability. Therefore, the drill bit chisel edge thinning design can effectively improve the drilling performance.