Bone Meal Samples Research Articles

Evaluation of 1064 nm Raman for meat and bone meal species discrimination: From raw form to chemical and physical components

The 1064 nm Raman spectroscopy technique was employed to characterize raw, bone fragments (BF), meat fragments (MF), lipid, and residue of meat and bone meal (MBM) samples. Employing three chemometric algorithms, species discrimination models were optimized for the aforementioned sample types, indicating that the presence of BF favored discriminant analysis for poultry samples, yielding a classification error of 0.000. The species discrimination model constructed using Raman spectra combined with PLS-DA algorithm for residue samples was found to be overall optimal, with classification error for four species samples all below 0.061. A detailed analysis of the BF related spectral variables contributing significantly to PLS-DA discriminant analysis was conducted. Finally, this study established the complementarity of Raman spectroscopy in species discrimination analysis from both a physical and chemical components perspective, laying the theoretical groundwork for the development of a high-precision 1064 nm Raman spectroscopic analysis method for MBM species discrimination.

Prevalence and antimicrobial resistance of Enterococcus spp. isolated from animal feed in Japan.

The rising prevalence of antimicrobial resistance (AMR) of bacteria is a global health problem at the human, animal, and environmental interfaces, which necessitates the "One Health" approach. AMR of bacteria in animal feed are a potential cause of the prevalence in livestock; however, the role remains unclear. To date, there is limited research on AMR of bacteria in animal feed in Japan. In this study, a total of 57 complete feed samples and 275 feed ingredient samples were collected between 2018 and 2020. Enterococcus spp. were present in 82.5% of complete feed (47/57 samples), 76.5% of soybean meal (62/81), 49.6% of fish meal (55/111), 33.3% of poultry meal (22/66), and 47.1% of meat and bone meal (8/17) samples. Of 295 isolates, E. faecium (33.2% of total isolates) was the dominant Enterococcus spp., followed by E. faecalis (14.2%), E. hirae (6.4%), E. durans (2.7%), E. casseliflavus (2.4%), and E. gallinarum (1.0%). Of 134 isolates which were tested for antimicrobial susceptibility, resistance to kanamycin was the highest (26.1%), followed by erythromycin (24.6%), tetracycline (6.0%), lincomycin (2.2%), tylosin (1.5%), gentamicin (0.8%), and ciprofloxacin (0.8%). All Enterococcus spp. exhibited susceptibility to ampicillin, vancomycin, and chloramphenicol. Of 33 erythromycin-resistant isolates, only two showed a high minimum inhibitory concentration value (>128 μg/mL) and possessed ermB. These results revealed that overall resistance to antimicrobials is relatively low; however, animal feed is a source of Enterococcus spp. It is essential to elucidate the causative factors related to the prevalence of AMR in animal feed.

Buffering Capacity Comparison of Tris Phosphate Carbonate and Buffered Peptone Water Salmonella Pre-Enrichments for Manufactured Feed and Feed Ingredients.

Various culture-based methods to detect Salmonella in animal feed have been developed due to the impact of this bacterium on public and animal health. For this project, tris phosphate carbonate (TPC) and buffered peptone water (BPW) buffering capacities were compared as pre-enrichment mediums for the detection of Salmonella in feed ingredients. A total of 269 samples were collected from 6 feed mills and mixed with the pre-enrichments; pH was measured before and after a 24 h incubation. Differences were observed when comparing pH values by sample type; DDGS and poultry by-product meal presented lower initial pH values for TPC and BPW compared to the other samples. For both TPC and BPW, meat and bone meal presented higher final pH values, while soybean meal and peanut meal had lower final pH values. Furthermore, for BPW, post cooling, pellet loadout, and wheat middlings reported lower final pH values. Additionally, most feed ingredients presented significant differences in pH change after 24 h of incubation, except DDGS. From meat and bone meal samples, four Salmonella isolates were recovered and identified: three using BPW and one using TPC. TPC provided greater buffer capacity towards neutral pH compared to BPW, but BPW was more effective at recovering Salmonella.

Contamination of Locally Compounded Poultry Feeds and Ingredients by Fungi of the Genus Penicillium and Aspergillus within Three Agro-Ecological Zones of Nigeria

Introduction: Fungi generally are field and storage contaminants of poultry feeds and feed ingredients, the fungal contamination of poultry feeds affects the quality negatively by causing caking, reducing quality and quantity of nutrients, and eventually producing secondary metabolites known as mycotoxins in these products when the environment is conducive. This study was carried out to isolate Aspergillus and Penicillium species from locally compounded poultry feed and feed ingredients that arrived at poultry farms within 30 days.
 Method: A total of 52 samples of feed (n=13) and feed ingredients (n=39) were collected across 3 agroecological zones (involving 6 states) of Nigeria. Different mycological media was used for isolation, by deploying the pour plate method. The identity of Aspergilli and Penicilli isolates was characterized using macroscopic and microscopic observed features.
 Results: Hundred per cent of the feed samples were contaminated with Aspergillus species while 63% were contaminated with Penicillium species. Nine species of Aspergillus and five species of Penicillium were detected in the feed and feed ingredients. The highest occurring fungal genus was Aspergillus, specifically, Aspergillus niger and Aspergillus flavus species were the highest occurring species. Penicillium glabrum was the most predominant Penicillium species isolated from the compounded poultry feed and feed ingredient samples.
 Conclusion: All the feed samples (100%) were contaminated with several species of Aspergillus and Penicillium. The feed ingredients had 100% contamination except for the bone meal samples 78% contamination.

Poultry feeds carry diverse microbial communities that influence chicken intestinal microbiota colonisation and maturation

Microbial colonisation of the gastrointestinal tract of newly hatched chicks starts at hatch, seeded from the immediate hatching environment, and quickly results in dense colonisation. The role of ecological factors in gut colonisation has been extensively investigated, as well as the role of micro- and macronutrients in supporting and selecting for bacterial species highly adapted for utilising those nutrients. However, the microbial community contained in poultry feed and its influence on colonisation and maturation of gut microbiota has not been directly addressed. In this study, we compared the microbiota found in poultry feed, with the microbiota of ileum, cecum and excreta, to identify substantial overlap in core microbiotas of the compared groups. We then investigated the microbiota present in raw feedstuffs: meat and bone meal, wheat, corn, canola, barley, soybean, millrun, sorghum, poultry oil, oats, limestone and bloodmeal from four geographically distinct feedstuff suppliers. Each of the feedstuffs had diverse microbial communities. The meat and bone meal and bloodmeal samples had the most complex and distinct microbial populations. There was substantial overlap in the phylogenetic composition found in the grain and seed samples: barley, canola, corn, millrun, oats, sorghum, soybean meal and wheat. Issues related to methodology, viability of microbial communities in the gut and feed, and the implications for biosecurity are discussed.

Multiplex PCR assay for animal species identification in meat bone meal

The authenticity of meat bone meal (MBM) in animal feed is needed to be considered due to inappropriate feed ingredients for certain types of livestock may cause various diseases. This work was aimed to identify animal species in MBM using multiplex PCR assay with 12S rRNA gene as a target region. Total of eight DNA samples were extracted from three species (chicken, bovine, and porcine) which are used as positive controls and three MBM samples. The MBM samples were collected from three different importers. Additionally, a multiplex PCR assay has been performed to identify animal species in MBM. Multiplex PCR of 12S rRNA gene was designed to detect bovine, chicken, and porcine in MBM samples. The PCR products was visualized using 2% agarose gels under the UV light. The results showed that multiplex-PCR of 12S rRNA gene was able to identify bovine which is indicated by 155 bp of DNA band. Chicken and porcine were not found in MBM samples. Meat bone meal samples used in this study might be used to be a feedstuff for poultry, porcine, and other non-ruminants. In conclusion, Multiplex-PCR using mt-DNA 12S rRNA was effective and accurate technique to identify species contained in MBM samples.

Chromium and lead contamination in commercial poultry feeds of Bangladesh

Heavy metals contamination into food chain is considered as an emerging crisis throughout the world especially in developing countries like Bangladesh. The concentration of heavy metals including Cr and Pb in commercial poultry feed samples (protein meal feed, meat and bone meal and fish meal feed) were detected by using Air/ Acetylene Flame Atomic Absorption Spectrophotometer. The mean concentration of heavy metals were found in protein meal feed in a range where Cr 10.63 to 218.10 mg kg-1 and Pb 7.37 to 52.25 mg kg-1. In meat and bone meal feed samples, Cr was recorded from 9.15 to 40.59 mg kg-1 and Pb 5.0 to 61.42 mg kg-1. Cr was found 17.68 to 78.39 mg kg-1 and Pb 3.54to 16.44 mg kg-1 in fish meal feed samples. However, results showed that all feed samples contained chromium and lead where both metals were present at alarming levels in most of the samples. It is therefore suggested that regular detection of heavy metals especially Cr and Pb should be done to evaluate the health risks and to protect the end user from food that might harmful for their health.Int. J. Agril. Res. Innov. & Tech. 6 (2): 57-60, December, 2016

Genotype Analysis of Bacillus anthracis Strains Circulating in Bangladesh.

In Bangladesh, anthrax, caused by the bacterium Bacillus anthracis, is considered an endemic disease affecting ruminants with sporadic zoonotic occurrences in humans. Due to the lack of knowledge about risks from an incorrect removal of infected carcasses, the disease is not properly monitored, and because of the socio-economic conditions, the situation is under-reported and under-diagnosed. For sensitive species, anthrax represents a fatal outcome with sudden death and sometimes bleeding from natural orifices. The most common source of infection for ruminants is ingestion of spores during grazing in contaminated pastures or through grass and water contaminated with anthrax spores. Domestic cattle, sheep and goats can also become infected through contaminated bone meal (used as feed) originating from anthrax-infected carcasses. The present investigation was conducted to isolate B. anthracis organisms from 169 samples (73 soil, 1 tissue, 4 bone and 91 bone meal samples) collected from 12 different districts of Bangladesh. The sampling was carried out from 2012 to 2015. Twelve samples resulted positive for B. anthracis. Biomolecular analyses were conducted starting from the Canonical Single Nucleotide Polymorphism (CanSNP) to analyze the phylogenetic origin of strains. The analysis of genotype, obtained through the Multiple Locus Variable Number Tandem Repeat Analysis (MLVA) with the analysis of 15 Variable Number Tandem Repeats (VNTR), demonstrated four different genotypes: two of them were previously identified in the district of Sirajganj. The sub-genotyping, conducted with Single Nucleotide Repeats analysis, revealed the presence of eight subgenotypes. The data of the present study concluded that there was no observed correlation between imported cattle feed and anthrax occurrence in Bangladesh and that the remarkable genetic variations of B. anthracis were found in the soil of numerous outbreaks in this country.

Development of a readily applied method to quantify ractopamine residue in meat and bone meal by QuEChERS-LC-MS/MS.

A QuEChERS method of ractopamine (RCT) residue detection in swine meat and bone meal (MBM) samples was demonstrated. Samples were hydrolyzed with protease and β-glucuronidase prior to QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction and clean-up. Samples were analyzed in a Liquid Chromatography (equipped with ACE 5 C18 column under gradient elution) coupled with a triple quadrupole mass spectrometer operating in positive electrospray ionization mode (using multiple reaction monitoring, MRM). The method was validated for its specificity, decision limit (CCα), detection capability (CCβ), recovery, repeatability, reproducibility, linearity, limits of detection (LODs), quantification (LOQs), and stability according to international guidelines (European Commission Decision 2002/657/EC). Recoveries ranged from 96.3 to 107.0%. Repeatability and reproducibility showed both RSD<5.7% and 3.1%, respectively. LODs and LOQs were 1.91 and 6.36 ppb, respectively. CCα and CCβ values were 1.91 and 2.37 ppb, respectively. RCT showed good stability for spiked samples and real samples when the concentration was higher, otherwise at lower concentration stability was lower. The proposed method can be successfully applied on a regular basis for the determination of RCT in MBM, demonstrating the usefulness of the method as a tool for compliance monitoring in regulatory laboratories.

Measurement of true ileal phosphorus digestibility in meat and bone meal for broiler chickens

An experiment was conducted to estimate true ileal phosphorus (P) digestibility of 3 meat and bone meal samples (MBM-1, MBM-2, and MBM-3) for broiler chickens. Four semipurified diets were formulated from each sample to contain graded concentrations of P. The experiment was conducted as a completely randomized design with 6 replicates (6 birds per replicate) per dietary treatment. A total of 432 Ross 308 broilers were assigned at 21 d of age to the 12 test diets. The apparent ileal digestibility coefficient of P was determined by the indicator method, and the linear regression method was used to determine the true P digestibility coefficient. The apparent ileal digestibility coefficient of P in birds fed diets containing MBM-1 and MBM-2 was unaffected by increasing dietary concentrations of P (P > 0.05). The apparent ileal digestibility coefficient of P in birds fed the MBM-3 diets decreased with increasing P concentrations (linear, P < 0.001; quadratic, P < 0. 01). In birds fed the MBM-1 and MBM-2 diets, ileal endogenous P losses were estimated to be 0.049 and 0.142 g/kg DM intake (DMI), respectively. In birds fed the MBM-3 diets, endogenous P loss was estimated to be negative (−0.370 g/kg DMI). True ileal P digestibility of MBM-1, MBM-2, and MBM-3 was determined to be 0.693, 0.608, and 0.420, respectively. True ileal P digestibility coefficients determined for MBM-1 and MBM-2 were similar (P < 0.05), but were higher (P < 0.05) than that for MBM-3. Total P and true digestible P contents of MBM-1, MBM-2, and MBM-3 were determined to be 37.5 and 26.0; 60.2 and 36.6; and 59.8 and 25.1 g/kg, respectively, on an as-fed basis.

Measurement of true ileal calcium digestibility in meat and bone meal for broiler chickens

The objective of the present experiment was to determine the true ileal calcium (Ca) digestibility in three samples of meat and bone meal (coded as MBM-1, MBM-2 and MBM-3) for broiler chickens. Four experimental diets, containing graded concentrations of Ca, were formulated from each MBM sample with inclusion levels of 20, 40, 60 and 80g/kg diet. Each experimental diet was randomly allotted to four replicate cages (six birds per cage) and fed for three days from day 28 post-hatch. Apparent ileal digestibility coefficient of Ca was measured by the indicator method and linear regression analysis was used to determine the true Ca digestibility coefficient. Apparent Ca digestibility was unaffected (P>0.05) by increasing Ca concentrations. The apparent digestibility coefficients in MBM-1, MBM-2 and MBM-3 were 0.50, 0.44 and 0.45, respectively. Significant linear (P<0.001) relationship was observed between dietary Ca intake and digesta Ca output for MBM-1, MBM-2 and MBM-3 with R2 values of 0.82, 0.93 and 0.95, respectively. The true ileal digestibility coefficients of Ca in MBM-1, MBM-2 and MBM-3 were determined to be 0.60, 0.46 and 0.50, respectively. The corresponding ileal endogenous Ca losses were 292, 123 and 174mg/kg dry matter intake, respectively.

Evaluation of the pepsin digestibility assay for predicting amino acid digestibility of meat and bone meals

Sixteen meat and bone meal (MBM) samples were obtained and selected from various company plants to provide a wide range in pepsin nitrogen digestibility values. Pepsin digestibility was determined using either 0.02 or 0.002% pepsin. Amino acid (AA) digestibility of the 16 MBM samples was then determined using a precision-fed cecectomized rooster assay. The 0.02% pepsin digestibility values were numerically higher than the 0.002% pepsin values. The values varied from 77 to 93% for 0.02% pepsin and from 67 to 91% for 0.002% pepsin. The rooster AA digestibility results showed a wide range of values among MBM samples mostly due to the 4 samples having lowest and highest AA digestibility. A precision-fed broiler chick ileal AA digestibility assay confirmed that there were large differences in AA digestibility among the MBM samples having the lowest and highest rooster digestibility values. Correlation analyses between pepsin and AA digestibility values showed that the correlation values (r) were highly significant (P < 0.0001) for all AA when all 16 MBM samples were included in the analysis. However, when the MBM samples with the 2 lowest and the 2 highest rooster digestibility values were not included in the correlation analyses, the correlation coefficient values (r) were generally very low and not significant (P > 0.05). The results indicated that the pepsin nitrogen digestibility assay is only useful for detecting large differences in AA digestibility among MBM. There also was no advantage for using 0.02 versus 0.002% pepsin.

The concentration of strontium and other minerals in animal feed ingredients

SummaryVariance in macro- and micro-mineral concentration in feed ingredients for farmed livestock contributes to sub-optimal performance and may compromise health and welfare. Although routine quality assurance and quality control procedures in feed mills or integrated poultry or swine businesses may track variance in the concentration of minerals of immediate nutritional importance, such as phosphorus (P), calcium (Ca) and sodium (Na), micro-minerals such as strontium (Sr) attract less attention. In order to create a framework for further study, the mineral concentration in more than 130 animal feed ingredients commonly used in Australia were analysed by inductively coupled plasma optical emission spectroscopy (ICP-OES). Due to a dearth of information, the principal focus of the survey was Sr, but the concentration of Ca, P, magnesium (Mg), manganese (Mn), potassium (K), iron (Fe), copper (Cu), zinc (Zn), sulphur (S) and Na were analysed concurrently. Generally the minerals present at the highest concentrations in the various feed ingredients examined were Ca, P and Mg. As anticipated, the ingredients with the highest concentrations of Ca and P were inorganic phosphates, limestone and meat and bone meal. The average Ca concentration in limestone was 393 g/kg but a range of 376–415 g/kg was observed which may be nutritionally important. Furthermore, the Mg concentration in limestone ranged from 7–535 mg/kg suggesting some contamination by dolomite lime sources. A total of 24 meat and bone meal samples were included in the analysis and mean Ca and P concentrations were 109 and 54 g/kg respectively. However, the range of Ca and P in meat and bone meal was considerable with Ca concentrations from 51–148 g/kg and P concentrations from 26–66 g/kg. A total of 81 cereal, grain legume and cereal by-product samples were included as part of the survey and these vegetable feed ingredients contained relatively low concentrations of most minerals with Ca, P, Mg and K dominating. The K concentration of soybean meal was found to be around 23 g/kg and ranged from approximately 22–27 g/kg. In comparison, the Sr concentration in the feed ingredients was low relative to other minerals, with limestone having the highest level of strontium at 329 mg/kg. Overall those feed ingredients from a mineral origin had the highest level of Sr. In addition, meat and bone meal had a relatively high concentration of Sr (around 159 mg/kg).

Metabolizable energy value of porcine meat and bone meal for broiler chickens

Bolarinwa, O. A., Olukosi, O. A. and Adeola, O. 2012. Metabolizable energy value of porcine meat and bone meal for broiler chickens. Can. J. Anim. Sci. 92: 73–78. Ross 308 broiler chickens at 14 d post-hatch were used in a 7-d experiment to determine the metabolizable energy (ME) value of two meat and bone meal (MBM) samples. Three hundred and thirty-six broiler chickens were grouped by weight into eight blocks of seven cages with six birds per cage. The seven diets consisted of a corn-soybean meal reference diet formulated to meet the NRC (1994) requirement, and six test diets. The reference diet contained four energy sources namely corn, soybean meal, corn starch and soy oil. In the test diets, each of the two MBM samples were added to the reference diet at 30, 60 or 90 g kg−1 diet to partly replace the energy sources such that the ratio of the energy sources to one another was kept constant in all the diets. The ME and nitrogen-corrected ME (MEn) of the MBM samples were determined by the regression method in which the MBM contribution to ME, and MEn of the diets, in kilocalories, were regressed against the amount of MBM intake in grams. Gross energy of MBM1 and MBM2 were 4247 and 4697 kcal kg−1 DM, respectively. The crude protein, ash, and crude fat contents for MBM1 or MBM2 were 567 or 594, 264 or 211, and 106 or 116 g kg−1 DM. Addition of MBM linearly decreased (P&lt;0.05) ileal nitrogen digestibility, total tract utilization of DM, energy and nitrogen as well as ME and MEn of the diets. In conclusion, the respective ME and MEn values were 2512 and 2345 kcal kg−1 DM for MBM1, and 2711 and 2127 kcal kg−1 DM for MBM2; thus MBM can be included in the diets of broiler chickens at less than 10% to provide energy.

Monitoring Salmonella sp dan Escherichia coli dalam Bahan Pakan Ternak (Salmonella sp and Escherichia coli Monitoring in the Animal Feed Ingredients)

&lt;p&gt;Quality of livestock feed affects production and animal health. Fish and bone meal is animal feed ingredients that need to be monitored because highly contaminated with of Salmonella sp and E. coli. Salmonella sp and E. coli are&lt;br /&gt;pathogen causing diarrhea in cattle and humans. The aim of this study was to investigate both E. coli and Salmonella contamination animal feed ingredients. A total of 38 animal feed ingredients samples from a poultry farm in Sukabumi&lt;br /&gt;consisting of 15 samples fish meal, bone meal 13 samples and corn 10 samples. These samples were analyzed for Salmonella sp and E. coli by isolation and identification using biochemical analysis. Salmonella Weltevreden was&lt;br /&gt;isolated from fish meal at rates of 6% from the 15 samples. E. coli was isolated at rates of 6% from the 15 fish meal samples and 7% from 13 bone meal samples, while corn samples were free from Salmonella sp and E. coli. This study&lt;br /&gt;showed that bone meal and fish meal was contaminated slightly by Salmonella Weltevreden and E. coli.&lt;/p&gt;&lt;p&gt;(Key words: Feed, Livestock, Salmonella sp, E. coli)&lt;br /&gt;&lt;br /&gt;&lt;/p&gt;

Quantification by Mass Of Residual Debris in Reusable Medical Devices

Quantification by Mass Of Residual Debris in Reusable Medical Devices

Machine Vision Detection of Bonemeal in Animal Feed Samples

There is growing public concern about contaminants in food and feed products, and reflection-based machine vision systems can be used to develop automated quality control systems. An important risk factor in animal feed products is the presence of prohibited ruminant-derived bonemeal that may contain the BSE (Bovine Spongiform Encephalopathy) prion. Animal feed products are highly complex in composition and texture (i.e., vegetable products, mineral supplements, fish and chicken meal), and current contaminant detection systems rely heavily on labor-intensive microscopy. In this study, we developed a training data set comprising 3.65 million hyperspectral profiles of which 1.15 million were from bonemeal samples, 2.31 million from twelve other feed materials, and 0.19 million denoting light green background (bottom of Petri dishes holding feed materials). Hyperspectral profiles in 150 spectral bands between 419 and 892 nm were analyzed. The classification approach was based on a sequence of linear discriminant analyses (LDA) to gradually improve the classification accuracy of hyperspectral profiles (reduce level of false positives), which had been classified as bonemeal in previous LDAs. That is, all hyperspectral profiles classified as bonemeal in an initial LDA (31% of these were false positives) were used as input data in a second LDA with new discriminant functions. Hyperspectral profiles classified as bonemeal in LDA2 (false positives were equivalent to 16%) were used as input data in a third LDA. This approach was repeated twelve times, in which at each step hyperspectral profiles were eliminated if they were classified as feed material (not bonemeal). Four independent feed materials were experimentally contaminated with 0-25% (by weight) bonemeal and used for validation. The analysis presented here provides support for development of an automated machine vision to detect bonemeal contamination around the 1% (by weight) level and therefore constitutes an important initial screening tool in comprehensive, rapid, and practically feasible quality control of feed materials.

Estimation of the metabolizable energy content of meat and bone meal for swine1,2

Experiments were conducted to determine apparent ME (AME) and apparent nitrogen-corrected ME (AME(n)) of 21 meat and bone meal (MBM) samples and to develop regression equations for predicting energy value of MBM. One hundred ninety-eight 32-kg of BW barrows were used for the study. The 22 treatments consisted of 1 corn-soybean meal reference diet and 21 test diets in which 21 MBM samples replaced 100 g/kg of corn and soybean meal such that the ratio of corn and soybean meal was the same in the reference and test diets. The DE, AME, and AME(n) of the MBM samples were determined by the difference method in a metabolism study consisting of 5-d adjustment and 5 d of total collection of feces and urine. On a DM basis, GE of MBM ranged from 3,895 to 5,193 kcal/kg, CP ranged from 491 to 641 g/kg, and ash ranged from 142 to 338 g/kg. The AME of the MBM samples ranged from 2,320 to 3,872 kcal/kg, whereas AME(n) ranged from 2,212 to 3,767 kcal/kg. None of the proximate compositions explained >50% of the variation in energy content of the MBM. Fat was positively correlated with GE, DE, AME, and AME(n) (r < or = 0.44), but CP, ash, Ca, and P were negatively correlated with DE, AME, and AME(n). The ratios of the proximate compositions to each other were correlated with the energy contents of the MBM. Crude protein:fat and GE:fat were negatively correlated with DE, AME, and AME(n) of the MBM (r ranged from -0.17 to -0.39), but fat:ash had the greatest positive correlation with AME and AME(n) compared with other ratios tested. When the data from 1 MBM sample that was an outlier were removed from the analysis, R(2) was 0.42 for AME and AME(n). The 4 variables that produced the best prediction equation for AME and AME(n) were GE, CP, P, and ash. The prediction equation for AME using these variables was AME = 13,587 - (1.25 x GE, kcal/kg) - (3.51 x CP, g/kg) + (30.4 x P, g/kg) - (16.4 x Ash, g/kg), and for AME(n), the equation was AME(n) = 13,547 - (1.25 x GE, kcal/kg) - (3.59 x CP, g/kg) + (31.0 x P, g/kg) - (16.5 x Ash, g/kg). It was concluded from this study that MBM is a good energy source for pigs and that, although other extrinsic factors may contribute to the variations in energy content of MBM, proximate compositions should be sufficient to predict the energy value of MBM for pigs.

Using Calibrations Developed for Fine Milled Meat and Bone Meal on Spectra Measured on Non-Milled Samples

A database of 316 near infrared (NIR) spectral data from finely milled meat and bone meal (MBM) samples were analysed in a Foss NIRSystems 6500 SY-II using the rectangular 1/4 cup. Calibration equations were developed for the prediction of crude protein and ash, two of the parameters used for quality control and payment in commercial MBM meal transactions ( R2CP = 0.96, SECVCP = 1.31; R2ashes = 0.94, SECVashes=0.91). Additionally, the possibility of using calibrations developed with fine milling samples to predict spectra obtained in the same instrument and analysed in the non-milled form, using the rectangular natural cup was examined. Three different standardisation sets were evaluated for optical matching of the samples analysed in the two different modes. The best standardisation matrix was applied to 10 validation samples. The predicted values from standardised spectra of non-milled samples analysed in the large cups and the predicted values from spectra of milled samples gave standard error of differences (SED) for CP (0.74) and ash (0.62) that were similar to, or even lower than, the standard error of prediction (SEP) values for the same milled samples scanned in the rectangular 1/4 cup (1.28 and 1.11, respectively). The study suggests that NIR equations developed for fine milled products analysed using a small window size may be used in the same instrument for the analysis of non-milled products analysed under a larger optical window.

Standardized Ileal Amino Acid Digestibility of Meat and Bone Meal from Different Sources in Broiler Chicks and Turkey Poults with a Nitrogen-Free or Casein Diet

The aim of this study was to determine the effect of the standardization method and age on standardized ileal amino acid digestibility (SIAAD) of 4 meat and bone meal (MBM) samples in broiler chicks and turkey poults. The SIAAD were obtained by correcting apparent ileal digestibility (AID) values for basal ileal endogenous amino acid flow obtained from chicks and poults fed a N-free diet or a highly digestible protein diet (HDP; 100 g of casein/kg of diet). Four diets were formulated to contain 20% CP by using MBM as the only source of CP. Each diet was fed to 6 replicate cages of 30 or 8 birds/cage at 5 and 21 d, respectively, for 5 d. With the exception of all-beef MBM, chick AID values on d 21 were higher (P < 0.05) than on d 5. In poults, however, AID was not different between the 2 ages, with the exception of His, which was higher (P < 0.05) at d 21. There was no difference between methods on SIAAD on d 5 or 21 for all 4 MBM samples in either species. There was no effect of the method of standardization on SIAAD at either age for most amino acids. For chicks, however, standardization of the all-beef MBM sample with HDP resulted in higher (P < 0.05) SIAAD values for most of the amino acids (d 5). In poults at d 5, however, the SIAAD for most of the amino acids in the blended MBM were higher (P < 0.05) with HDP correction. The CV in SIAAD in chicks between the 2 methods of standardization was higher on d 5 (8.1%) relative to d 21 (5.0%) for all treatments. In poults, greater variability was observed on d 21 (8.2%) relative to d 5 (6.7%). Results from this study showed that at d 21, SI-AAD with either the N-free or the HDP diet resulted in similar digestibility values.