Bohadschia Argus Research Articles

Enzyme-Assisted Extraction of Fucosylated Chondroitin Sulfate from Sea Cucumber Holothuria scabra and Bohadschia argus and their Potential in Pharmaceutical Applications

Due to the health benefit of fucosylated chondroitin sulfate (FuCS), the efficient method for extraction of FuCS from raw materials is a crucial issue in reducing the production cost. In this study, enzymatic extraction of FuCS from two species of sea cucumber, Holothuria scabra and Bohadschia argus was undertaken using two protease enzymes, alcalase and papain. Response surface methodology (RSM) was employed in determining the optimal extraction conditions with the highest yield of FuCS concentration. The predicted optimal papain-assisted extraction conditions of Holothuria scabra and Bohadschia argus obtained a predicted FuCS yield of 1609.73 mg/100 g and 444.51 mg/100 g, respectively. To compare extraction efficiencies of two protease enzymes, employment of the RSM optimal conditions to Holothuria scabra resulted in 1538.76 ± 20.26 mg/ 100 g and 1295.50 ± 14.28 mg/100 g of purified FuCS for papain and alcalase, respectively. Whereas Bohadschia argus produced 412.39 ± 10.12 mg/100 g and 461.11 ± 8.45 mg/100 g purified FuCS for papain and alcalase, respectively. The acquired FTIR and NMR spectrums of extracted FuCS showed typical bands of sulfation patterns and were compared to commercial FuCS. The extracted FuCS showed enzyme type dependent antioxidant activity, and significant tyrosinase inhibitory activity than commercial FuCS. It also exhibited similar anti-glucosidase activity as commercial FuCS. Thus, this study reveals potential applications of enzyme-assisted FuCS from sea cucumber in food and pharmaceutical industries.

Bioecology study of Holothuroidea (sea cucumbers) in the northern coastal area of Timor Island, East Nusa Tenggara

Holothuroidea is one of the marine biotas with crucial economic value in Asia and is usually served fresh or dried in various cuisines. Although it has significant market demand, the potential of sea cucumbers in the northern part of Timor Island has yet to be investigated. This study was carried out to understand existing bioecological conditions and investigate the location’s suitability to support the development of sea cucumber farming in this area. The method employed in the bioecology study followed a line transect and quadrants perpendicular to the beach. Various water quality measurements were applied to quantify the location suitability index. The results observed four species in the Poanboun coastal area: Holothuria leucospilota, H. hilla, Bohadschia argus, and H. atra (the order indicates the highest to lowest density and abundance). The substrate types in the area were a combination of sandy, muddy sand, coral fragments, and surrounded sea grapes macroalgae. The region has a high location suitability index where secluded area, water depth and bed, temperature, salinity, pH, DO, and wave conditions are prime indicators to support the suitability, followed by water clarity and current as secondary (quite supportive indicators).

Oligomer-guided recognition of two fucan sulfate from Bohadschia argus and inhibition of P-selectin binding to its ligand

Fucan sulfate (FS) from sea cucumber shows intriguing structure and extensive activities. Here, three homogeneous FS (BaFSI – III) were obtained from Bohadschia argus, followed with physicochemical properties analyses including monosaccharide composition, molecular weight, and sulfate content. BaFSI was proposed to carry a unique distribution pattern of sulfate groups as a novel sequence composed of domain A and domain B that formed by different FucS residues, markedly differing from FS reported before, according to the analyses of 12 oligosaccharides and a representative residual saccharide chain. BaFSII possessed a highly regular structure {4-L-Fuc3S-α1,}n according to its peroxide depolymerized product. BaFSIII was confirmed as a FS mixture bearing similar structural characteristics with BaFSI and BaFSII by means of mild acid hydrolysis and oligosaccharide analysis. Bioactivity assays showed that BaFSI and BaFSII could potently inhibit P-selectin binding to PSGL-1 and HL-60 cells. Structure-activity relationship analysis showed that molecular weight and sulfation pattern were the essential factors for the potent inhibition. Meanwhile, an acid hydrolysate of BaFSII with a molecular weight about 15 kDa exhibited a comparable inhibition with the native BaFSII. Given the potent activity and highly regular structure of BaFSII, it shows great potential for development as a P-selectin inhibitor.

Analysis of Complete Mitochondrial Genome of Bohadschia argus (Jaeger, 1833) (Aspidochirotida, Holothuriidae).

Simple SummaryMitochondrial genomes are a type of specific genetic marker, characterized in most animals by a rapid mutation rate, high copy numbers and the absence of genetic recombination. They are widely applied in phylogenetics, population genetics and ecological research. Bohadschia argus is a sea cucumber with high economic value. Currently, the mitochondrial genomic data of B. argus are not available. In this study, next-generation sequencing was performed on the mitochondrial genome of B. argus. The gene arrangement order in the mitochondrial genome of B. argus was consistent with the echinoderm ground pattern. Finally, phylogenetic analysis using the 13 protein-coding genes of mitochondria confirmed the phylogenetic position of B. argus.Bohadschia argu is a kind of sea cucumber with high economic value; it is the only undisputed species in the genus Bohadschia. In this study, the complete mitochondrial genome (mitogenome) of B. argus was acquired through high-throughput sequencing. The mitochondrial genome of B. argus was 15,656 bp in total length and contained a putative control region (CR) and 37 typical genes of animal mitochondrial genomes, including 13 protein-coding genes (PCGs), 2 ribosomal RNA genes (rrnS and rrnL) and 22 transfer RNA genes (tRNA). The sizes of the PCGs ranged from 168 bp to 1833 bp, and all PCGs except nad6 were encoded on the heavy chain (H). Both rrnS and rrnL were also encoded on the H chain. Twenty-two tRNA genes had positive AT skew and GC skew. All tRNAs had a typical cloverleaf secondary structure except for trnI, in which an arm of dihydrouridine was missing. B. argus shared the same gene arrangement order (the echinoderm ground pattern) as other species in Aspidochirotida. Phylogenetic analysis clearly revealed that B. argus belongs as a member of the Holothuriidae, and it is closely related to members of Actinopyga and Holothuria.

Optimization of Enzyme Assisted Extraction of Chondroitin Sulfate from Bohadschia argus by Response Surface Methodology

Chondroitin sulfate is currently one of the bioactive compounds obtained from different natural materials with high benefit in human medical treatment and pharmaceutical. However, the natural concentration of chondroitin sulfate in high cost raw material is low and this situation makes the commercialization become difficult. This study focused on production of chondroitin sulfate isolated from Bohadschia argus using enzyme-assisted extraction methodology. Optimization experiments were conducted based on Response Surface Methodology (RSM) using Box–Behnken design (BBD). Three important extraction parameters, including enzyme concentration (X1: 0.05-0.5 %), Time (X2: 0.25–3 h), and temperature (X3: 55–65 °C) were varied to obtain maximum chondroitin sulfate yields. The RSM optimized model obtained from statistical analysis presented the high correlation coefficiency (R2) at 0.7508, advocating the significance of the model. The optimum extraction conditions were selected as enzyme concentration of 0.48% with extraction time of 1.01 h. at an extraction temperature of 56.53°C. Under these conditions, the experimental yield of chondroitin sulfate was 415.59 mg/100g dry, which is in good agreement with the value predicted by the model.

Anti-Fouling Effects of Saponin-Containing Crude Extracts from Tropical Indo-Pacific Sea Cucumbers.

Sea cucumbers are bottom dwelling invertebrates, which are mostly found on subtropical and tropical sea grass beds, sandy reef flats, or reef slopes. Although constantly exposed to fouling communities in these habitats, many species are surprisingly free of invertebrate epibionts and microfouling algae such as diatoms. In our study, we investigated the anti-fouling (AF) activities of different crude extracts of tropical Indo-Pacific sea cucumber species against the fouling diatom Cylindrotheca closterium. Nine sea cucumber species from three genera (i.e., Holothuria, Bohadschia, Actinopyga) were selected and extracted to assess their AF activities. To verify whether the sea cucumber characteristic triterpene glycosides were responsible for the observed potent AF activities, we tested purified fractions enriched in saponins isolated from Bohadschia argus, representing one of the most active anti-fouling extracts. Saponins were quantified by vanillin-sulfuric acid colorimetric assays and identified by LC-MS and LC-MS/MS analyses. We were able to demonstrate that AF activities in sea cucumber extracts were species-specific, and growth inhibition as well as attachment of the diatom to surfaces is dependent on the saponin concentration (i.e., Actinopyga contained the highest quantities), as well as on the molecular composition and structure of the present saponins (i.e., Bivittoside D derivative was the most bioactive compound). In conclusion, the here performed AF assay represents a promising and fast method for selecting the most promising bioactive organism as well as for identifying novel compounds with potent AF activities for the discovery of potentially novel pharmacologically active natural products.

Skrining Senyawa Kimia dan Uji Aktivitas Sitotoksik Ekstrak Etanol Teripang Kridou Bintik (Bohadschia argus Jeager) Asal Pantai Harlem Kabupaten Jayapura, Papua

The Bohadschia argus Jeager contains bioactive compounds that have the potential as antibiotics, antibacterial, antitumor, anticoagulants and anesthetics and alleviate cancer. This study aims to determine the content of chemical compounds group and to test the cytotoxic activity of ethanol extract of Bohadschia argus Jeager. The method for determining the group of chemical compounds was used the color reaction and precipitation using chemical reagents. The method for testing cytotoxic activity using the Brine Shimp Lethality Test (BSLT), this method commonly was used to test the safety of the use of natural ingredients as traditional medicine. The results of screening of the chemical compound group of  Bohadschia argus Jeager extract showed that the extract contained a lot of alkaloids secondary metabolites and few saponins. The results of testing of cytotoxic activity showed LC50 value of 878.22 ppm. Base on the results of this study it can be concluded that the ethanol extract of Bohadschia argus Jeager has significant cytotoxic activity against Artemia salina Leach, so it can be considered as a chemotherapeutic agent.   Key words: Screening, chemical compounds, ethanol extract, B. argus, BSLT.Â

Role of Active Compounds of Bohadschia argus Inhibit Cancer Cell Survival

Sea cucumber is marine biota with a high economic value and also has potential for anti-cancer. The purpose of this study was to explore the mechanism of active compound of Bohadschia argus on regulating cancer cell survival. The B. argus samples were collected from the sea of Kamal Village, West Seram Maluku, then extracted by water. The constituents of water extract of B. argus were examined by LC-MS. The network among active compound and its protein target were determined by Cytoscape app. The result shows that B. argus has several active compounds, such as chondroitin sulfate, holothurin A, holothurin B, and scabraside that might play a role in cancer cell apoptosis, proliferation, and metastasis. Keywords: Active compound , Bohadschia argus , LC-MS.

Identification of Beche-de-mers from Indonesia by molecular approach

Abstract. Patantis G, Dewi AS, Fawzya YN, Nursid M. 2019. Identification of Beche-de-mers from Indonesia by molecular approach. Biodiversitas 20: 537-543. Sea cucumbers have been commercially traded around the world due to their nutritional values and health benefits. Sea cucumbers are marketed as processed sea cucumber, also known as beche de mer, teripang, haisen or gamat. Due to the high global demand of beche-de-mers, there have been several cases of fraud due to the visual shape and color similarities of processed sea cucumbers. Additionally, the local name diversity of Indonesian sea cucumbers making the identification of beche-de-mers difficult. Therefore, this study aimed to identify commercial sea cucumbers from selected locations in Indonesia by molecular approach. The targeted Cytochrome c oxidase subunit I (COI) was used in this research. A total of 25 beche-de-mers was purchased from local markets in Indonesia, i.e. Boalemo (17 local names), Pesawaran (5), Surabaya (2) and West Lombok (1). Results showed that 22 species of beche-de-mer were successfully identified, ie. Acaudina sp., Actinopyga echinites, A. lecanora, A. miliaris, Bohadschia argus, B. marmorata, B. ocellata, B. vitiensis, Holothuria sp. H. atra, H. coluber, H. edulis, H. excellens, H. fuscocinerea, H. fuscogilva, H. fuscopunctata, H. scabra, Pearsonothuria graeffei, Phyrella sp., Stichopus herrmanni, S. horrens and S. monotuberculatus. The identification of Indonesian beche-de-mers using molecular method could support the identification of the local sea cucumbers to overcome mislabeled and fraud. Correct identification is important for the record of beche-de-mer trade in Indonesia and further conservation management.

FAKTOR FISIK KIMIA LINGKUNGAN PENDUKUNG KEANEKARAGAMAN DAN KEMERATAAN HOLOTHUROIDEA PADA ZONA INTERTIDAL DESA HILA KECAMATAN KEPULAUAN ROMANG KABUPATEN MALUKU BARAT DAYA

Background: The sea is an ecosystem that has high biodiversity. Hila Village is one area that has a large enough waters located in the western part of Romang island, District of Romang Islands, Southwest Maluku District. Intertidal Zone Hila Village has different types of substrate, both muddy, sandy, sandy, dreamy, rocky, rocky, sandy, rocky, and rocky rocked. The existence of the types of sea cucumbers (Holothuroidea) in the coastal waters of Hila village quite a lot, physical chemical environmental factors in the coastal waters of Hila Village support the life of the sea cucumber.
 Method: This research is descriptive research that reveal information about environmental characteristic (temperature, pH, salinity, and dissolved oxygen), diversity and evenness of sea cucumber in intertidal zone of Hila Village.
 Results: The results of the research on the intertidal zone of Hila Village, Pulau Romang Subdistrict, Southwest Maluku District, were found 7 genus and 8 spesies. The types of Holothuroidea found in the study sites are Bohadschia argus, Holothuria atra, Holothuria edulis, Bohadschia marmorata, Holothuria scraba, Chiridota violaceae, Holothuria fuscopunctata, and Opheodesoma spectabili. The Holothuroidea diversity index in the Intertidal Zone of Hila Village is 1.56, categorized by medium diversity, for evenly distributed evenness because the fairness index is 1.05.
 Conclusion: The index of the Holothuroidea diversity in the Intertidal Zone of Hila Village is 1.56, categorized by medium diversity, for evenly distributed evenness because the fairness index is 1.05. Chemical Physical Factors The envelope found was the average temperature of 28.270C, the average pH 6.373, and the average salinity of 4.94%, indicating that the Holothuroidea was able to adapt and live in the intertidal zone of Hila Village.

Recovery rates for eight commercial sea cucumber species from the Fiji Islands

Determination of the original weight and length of sea cucumbers processed and dried to become bêche-de-mer (BDM), is an important tool in sea cucumber fishery management. The only management mechanism for the sea cucumber fishery in the Fiji Islands is a minimum length prescribed for BDM for export. However, different commercial species have different shrinkage rates during processing and previous studies have suggested modification of fisheries management for sea cucumbers to include species-specific minimum harvest size limits This study determined weight-based and length-based recovery rates (i.e. the length/weight of BDM recovered after processing from the initial length/weight of fresh sea cucumber), for eight commercial sea cucumber species following processing to BDM; White Teatfish (Holothuria fuscogilva), Black Teatfish (Holothuria whitmaei), Tigerfish (Bohadschia argus), Surf Redfish (Actinopyga mauritiana), Hairy Blackfish (Actinopyga miliaris), Stonefish (Actinopyga lecanora), Prickly Redfish (Thelenota ananas) and Sandfish (Holothuria scabra). Length and weight recovery rates varied between species and ranged from the highest recovery values of 54.9% for length and 11% for weight in Black Teatfish, to the lowest recovery values of 32.6% for length and 3.0% for weight in Sandfish and Tigerfish, respectively. Length-based and weight-based relationships were generated for each species through the various stages of processing from fresh to dried (BDM) allowing estimation of initial fresh weight/length from partially or fully processed BDM and vice versa. Information generated in this study provides a basis for developing more species-specific harvest size restrictions for sea cucumbers in the Fiji Islands, and has application in stock assessment studies, estimation of harvest data, monitoring of harvest size limits and standardizing catch data.

Chemical Composition and Fatty Acid Profile of Some Indonesian Sea Cucumbers

Indonesia has a great sea cucumber resource which is useful as functional food. Even though much information about the biopotency of sea cucumbers have been published, however, studies on nutrition value of Indonesian sea cucumbers are still beneficial due to the great diversity and uniqueness of each species. The present work was intended to obtain information about chemical composition and fatty acid profile of 4 (four) types of sea cucumbers which are frequently found in Halmahera water, North Maluku (Molucca), one of sea cucumbers producing area in Indonesia. The samples coded as H-03, H-04, H-05 and H-10, were identified as Bohadschia argus, Holothuria fuscogilva, Thelenota ananas, and Actinophyga lecanora. All sea cucumbers showed high protein content, more than 60% (drybase/db), except for T. ananas which had the lowest protein content (48.26% db). As for fats, T. ananas showed the highest amount (2.35%db) with dominated saturated fatty acids (SFA). On the other side, A. lecanora showed higher value of PUFA compared to SFA and MUFA. Palmitic acid (C16:0) was the most abundant SFA in the most samples with the amount of 0.49–4.9 mg/g sample. Among PUFA, eicosapentaenoic acid/EPA (C20:5n3) was detected, and eicosatrienoic acid (C20:3n6) was relatively higher than the others. The ratio of w6/w3 fatty acid was 1.2–3.2 showing that total w6 fatty acid was higher than total w3 fatty acid. However, this value is safe enough to protect against chronic and degenerative diseases.

DIVERSITY OF SEA CUCUMBER (ECHINODERMATA: HOLOTHUROIDEA) IN SABU RAIJUA WATERS, SABU ISLAND, EAST NUSA TENGGARA

Sea cucumber has an ecological function as an organic decomposer in sediment and nutrient producer within a food chain. In addition, sea cucumber has also economic values as fisheries and trade commodities. The pupose of this research was to investigate diversity of sea cucumber in the Sabu Raijua waters, East Nusa Tenggara Province. Samples were collected from Sabu Raijua waters in July -August 2014 using transect quadrant of 1x1 m2 during the lowest low tide in daytime at 13:00-16:00 and nighttime at 18.00-22.00 Central Standar Local Time. Data analyses on the sea cucumber were based on composition, density, distribution, and eating habits. The results showed that there were 8 species that can be classified into 3 families i.e., the Holothuridae (Holothuria nobilis, Holothuria scabra, Holothuria atra, Holothuria edulis, Holothuria impatiens, and Holothuria leucospilato); the Actinopyga (Actinopyga lecanora); and the Bohadshia (Bohadschia argus). The density of Holothuria nobilis was 5.651 individual/m2, Holothuria atra of 4.409 individual/m2, Holothuria scabra of 3.294 individual/m2; Holothuria edulis of 3.102 individual/m2; Bahaschia argus of 2.102 individual/m2; Holothuria mexicana of 2.088 individual/m2; Holothuria impatiens of 2.044 individual/m2; and Actinopyga lecanora of 1.037 individual/m2. Of these sea cucumber species, 17.3% were distributed on sandy substrate and 82.7% on seagrass and coral subtsrates. All sea cucumber species have a posi-tive association with its environment indicating that the water quality was still in a good condition. Keywords: sea cucumber, association, diversity, composition, distribution

Community Structure of Cucumber (Holothuroidea) in Kakara Island Village Beach Subdistrict Tobelo

Sea cucumbers are marine resources that have economically important value and one potential sufficient resources to serve nutritious meals so that the exploitation of this resource will continue to increase for food need. This can lead to changes in community structure. Sampling was done at night while the lowest ebb on August 20, 2013. The objective of this research work was to identity the species, the species to determine composition, and the population density and to know the species dominance, species diversity, and determine the form of interspecific association. Based on the results of research conducted in the District Tobelo Beach Island District Kakara Tobelo sea cucumber species found were 8 species, Holothuria atra, Actinopygia lecanora, Stichopus horrens, Bohadschia argus, Holothuria hilla, Actinopygia achinites, Holothuria pardalis, and Bohadschia marmorata. The diversity index H '= 1.9463, equity index or harmony e = 0.9359, and dominance index C = 0.1592. In addition, among 8 species associations if formed 9 pairs of positive associations and 19 pairs of negative associations. Keywords : Structure communities, Holothuroidea, Tobelo

Antibacterial Activity of Sea Cucumbers Harvested from Karimunjawa

This research was conducted to discover bioactive compounds in sea cucumbers collected from Karimunjawa as anti-microbial agents against several pathogenic bacteria. Five sea cucumbers species used in this study were Teripang Gamat (Stichopus variegatus), Teripang Nanas (Stichopus chloronotus), Teripang Getah (Bohadschia mamorata), Teripang Emas (Stichopus herrmanni) and Teripang Babi (Bohadschia argus). Sea cucumbers extract were subjected to a series of anti-microbial tests using Staphylococcus aureus, Escherichia coli, Vibrio anguila, Vibrio voinivica, Bacillus subtilis, and Pseudomonas sp. Results showed that in the preliminary study, tissue extract of Bohadschia mamorata and Bohadschia argus were able to suppress the growth of all tested bacteria. Further study used these two sea cucumber extracts, and the results show that Bohadschia mamorata extract at 10 mg/ml showed the largest antibacterial (2.18 mm) towards the pathogenic bacterium Pseudomonas sp., while the smallest antibacterial zone (0.63 mm) was against the growth of Bacillus subtilis. At the concentration level of 20 mg/ml Bohadschia argus extract showed the highest antibacterial zone of 3.68 mm against Staphylococcus aureus and the lowest inhibition zone (1.75 mm) against Pseudomonas sp.

TOXICITY TEST OF EXTRACTS OF THE FOUR SEA CUCUMBER (FAMILY HOLOTHURIIDAE) FROM EAST PENJALIRAN ISLAND, SERIBU ISLANDS, JAKARTA BASED ON THE BRINE SHRIMP LETHALITY TEST (BSLT)

<p>Sea cucumber is one of the marine bioactive compounds resources . The properties of the bioactive compound are biologically shown by their functions as anticancer, antifungi, hemolytic, and immunomodulatoryt. The purpose of this research is to predict (determine) the toxicity level of the extracts of Actinopyga miliaris, Holothuria leucospilota, Bohadschia argus, and Bohadschia marmorata collected from Penjaliran Timur Island, Seribu Island National Park Jakarta. The method used in this research is Brine Shrimp Lethality Test (BSLT). The result of this research showed that all of the extract from those species are active in BSLT, indicated by LC50 values of less than 1000 μg/ml. Bohadcshia argus is highest active species indicated by LC50 value 69,254 μg/ml. Water fraction of H. leucospilota’s crude extract is the most active fraction indicated by LC50 50,968 μg/ml.</p><p>Keywords: Brine Shrimp Lethality Test, Crude Extract, Fractionation, Family Holothuriidae, Sea Cucumber</p>

TOXICITY TEST OF EXTRACTS OF THE FOUR SEA CUCUMBER (FAMILY HOLOTHURIIDAE) FROM EAST PENJALIRAN ISLAND, SERIBU ISLANDS, JAKARTA BASED ON THE BRINE SHRIMP LETHALITY TEST (BSLT)

Sea cucumber is one of the marine bioactive compounds resources . The properties of the bioactive compound are biologically shown by their functions as anticancer, antifungi, hemolytic, and immunomodulatoryt. The purpose of this research is to predict (determine) the toxicity level of the extracts of Actinopyga miliaris, Holothuria leucospilota, Bohadschia argus, and Bohadschia marmorata collected from Penjaliran Timur Island, Seribu Island National Park Jakarta. The method used in this research is Brine Shrimp Lethality Test (BSLT). The result of this research showed that all of the extract from those species are active in BSLT, indicated by LC50 values of less than 1000 μg/ml. Bohadcshia argus is highest active species indicated by LC50 value 69,254 μg/ml. Water fraction of H. leucospilota’s crude extract is the most active fraction indicated by LC50 50,968 μg/ml.Keywords: Brine Shrimp Lethality Test, Crude Extract, Fractionation, Family Holothuriidae, Sea Cucumber

Chemical composition and nutritional quality of sea cucumbers

The dried form of sea cucumbers has been a seafood and medicinal cure for Asians over many centuries. In this study the chemical composition and nutritional quality of eight common sea cucumbers (Stichopus herrmanni, Thelenota ananas, Thelenota anax, Holothuria fuscogilva, Holothuria fuscopunctata, Actinopyga mauritiana, Actinopyga caerulea and Bohadschia argus) were determined. All species except T. anax and A. caerulea had higher protein and lower fat levels. A. mauritiana and B. argus had less ash content. Glycine was the dominant amino acid found in all species, and content ranged from 126 to 216 mg g(-1) of crude protein. All species exhibited low lysine:arginine ratio and higher essential amino acid scores were obtained by threonine and phenylalanine + tyrosine. A. mauritiana had proportionally less saturated fatty acids (31.23%), and more monounsaturated fatty acids (45.64%) and polyunsaturated fatty acids (PUFA, 23.13%) than other species. Arachidonic acid (C20:4n-6) was the major PUFA in all species. T. ananas, A. mauritiana and A. caerulea contained more n-3 PUFA. The n-3/n-6 ratios of eight sea cucumbers species ranged from 0.25 to 0.61. Sea cucumbers are a seafood with high protein and low fat levels. The amino acid contents were similar but fatty acid profiles were different among species. The comparison showed that T. ananas, A. mauritiana and B. argus possessed higher nutritional values than other sea cucumber species.

Langmur monolayers of cerebroside with different head groups originated from sea cucumber: Binary systems with dipalmitoylphosphatidylcholine (DPPC)

Surface properties (Langmuir monolayer) of two different cerebrosides which are extracted from the sea cucumber ( Bohadschia argus) were investigated. A main difference in chemical structure of cerebroside between BAC-2a and BAC-4 is their head groups (glucose and galactose, respectively). Furthermore, miscibility and interaction between dipalmitoylphosphatidylcholine (DPPC) and cerebrosides (BAC-2a and BAC-4) in the monolayer have been systematically examined. The surface pressure ( π)−area ( A), the surface potential (Δ V)− A, and the dipole moment ( μ ⊥)− A isotherms for monolayers of DPPC, cerebrosides, and their binary combinations have been measured using the Wilhelmy method and the ionizing electrode method. BAC-4 forms a stable liquid-expanded (LE) monolayer, whereas BAC-2a has a first-order phase transition from the LE phase to the liquid-condensed (LC) state on 0.15 M NaCl at 298.2 K. The fundamental properties for each cerebroside monolayer were elucidated in terms of the surface dipole moment based on the three-layer model [R.J. Demchak, T. Fort Jr., J. Colloid Interface Sci. 46 (1974) 191–202] for both cerebrosides and the apparent molar quantity change (Δ s γ , Δ h γ , and Δ u γ ) for BAC-2a. In addition, their miscibility with DPPC was examined by the variation of the molecular areas and the surface potentials as a function of cerebroside mole fractions, the additivity rule. The miscibility was also confirmed by constructing the two-dimensional phase diagrams. The phase diagrams for the both binary systems were of negative azeotropic type. That is, the two-component DPPC/BAC-2a and DPPC/BAC-4 monolayers are miscible. Furthermore, the Joos equation for the analysis of the collapse pressure of binary monolayers allowed calculation of the interaction parameter and the interaction energy between the DPPC and cerebroside monolayers. The miscibility in the monolayer state was also confirmed by the morphological observation with Brewster angle microscopy (BAM), fluorescence microscopy (FM), and atomic force microscopy (AFM).

Isolation and Structure of a Galactocerebroside from the Sea Cucumber Bohadschia argus

A galactocerebroside (BAC-4-4a) was obtained from its parent galactocerebroside molecular species (BAC-4) by using two types of reversed-phase HPLC column. The parent cerebroside BAC-4 has been isolated from less polar lipid fraction of CHCl(3)/MeOH extract of sea cucumber Bohadschia argus, together with known typical types of glucocerebroside molecular species. The structure of BAC-4-4a was determined on the basis of chemical and spectroscopic evidences as 1-O-(beta-D-galactopyranosyl)-(2S,3R,4E)-2[(2'R,15'Z)-2-hydroxytetracosenoylamino]-4-heptadecene-1,3-diol. This is the first report of isolation and structure determination of galactocerebroside from sea cucumber.