Boer Bucks Research Articles

Effects of dietary Kleinhovia hospita and Leucaena leucocephala leaves on rumen fermentation and microbial population in goats fed treated rice straw

The effects of partial replacement of dietary protein by forages on rumen fermentation and microbiology in goats were examined. Four fistulated Boer bucks were used in a 4 × 4 Latin square design. The goats were fed 60% of urea-treated rice straw and 40% dietary treatment (Kleinhovia hospita (KH), Leucaena leucocephala (LL), mixture of K. hospita with L. leucocephala (KHLL)) and concentrate as the control. Rumen fluid from the animals was collected at 0, 2, 4, 6, and 12 h postprandial for analysis. The KHLL diet had a greater (P < 0.05) molar proportion of acetate than the control diet throughout the sampling period. At 6 h postprandial, the KHLL goats had a significantly lower (P < 0.05) ammonia nitrogen than the goats fed other diets. The molar proportion of propionate (24.7 and 25.8 mol/100 mol) was greater in the rumen of KHLL goats compared with those fed other diets at 2 and 12 h postprandial, respectively. The KHLL diet had lower (P < 0.05) butyrate than other dietary treatments. At 4 h postprandial, the control goats had a lower (P < 0.05) population of total bacteria while the KHLL goats had a greater (P < 0.05) population at 4 and 12 h postprandial compared with those fed other diets. The LL, KH, and KHLL goats had lower (P < 0.05) populations of protozoa and methanogens and a greater (P < 0.05) population of Ruminococcus albus compared with the control goats. The KHLL leaves could be fed to goats without compromising rumen metabolism.

Association of novel SNPs in gonadotropin genes with sperm quality traits of Boer goats and Boer crosses

ABSTRACTThe present study aimed to investigate single-nucleotide polymorphisms (SNPs) of caprine FSHβ and caprine LHβ genes and to determine effects of the SNPs on fresh and post-thaw semen quality traits of Boer and Boer-crossbred bucks. The single-strand conformation polymorphism analysis and comparative sequencing revealed three SNPs in exon 3 of caprine follicle-stimulating hormone beta (FSHβ), including, three SNPs; 200A > G (FSHβ3-a), 226T > C (FSHβ3-b), and 237A > G (FSHβ3-c), while two of them (FSHβ3-a and FSHβ3-b) were novel. Furthermore, one SNP in exon 2 of luteinizing hormone beta (LHβ) (207T > C) (LHβ2) was detected. The associations of the four detected SNPs with quality traits of fresh and post-thaw semen were evaluated. Analyses of variance revealed significant association of the candidate genes with libido and semen quality traits. The three SNPs of FSHβ3 had significant effect on libido, progressive motility, and abnormality of fresh semen (P < .05), and on the motility, velocity, and viability traits of post-thaw semen (P < .05). LHβ2 polymorphism only showed association with sperm viability of post-thaw semen. The results of the present study suggest that the SNPs in caprine FSHβ and LHβ are associated with semen quality of male goats.

SELECTION OF BREEDING STOCK BY U.S. MEAT GOAT PRODUCERS

AbstractUsing nationwide survey data, we investigate U.S. meat goat producer preferences and willingness to pay for meat goat breeding stock attributes. Discrete choice experiments were employed, and mixed logit and latent class models were used for analysis. Results showed that producers preferred animals that were highly masculine/feminine, had good structure and soundness, and were of the Boer breed, whereas they preferred fewer animals that were older, of Kiko and Spanish breeds, and priced higher. Significant preference heterogeneity was found among the respondents. Larger-scale producers had greater preference for high masculinity/femininity, good structure and soundness, and Boer bucks.

Rumen microbial community and nitrogen metabolism in goats fed blend of palm oil and canola oil

This study assessed the influence of dietary blend of 20% palm oil and 80% canola oil on the population of rumen microbiota and nitrogen economy in goats. Twenty-four Boer bucks (4–5 months old; initial BW, 20.54 ± 0.474 kg) were randomly allotted to diets containing on a dry matter basis, 0, 4 or 8% oil blend, fed daily for 100 days and slaughtered. The rumen microbiota was examined by quantitative real-time polymerase chain reaction (PCR) using the 16S rRNA gene. The population of total protozoa and methanogens was lower (p < .05) while the population of Fibrobacter succinogenes was greater (p < .05) in goats fed oil blend compared with the control goats. The population of total bacteria was greater (p < .05) in goats fed 8% oil blend compared with those fed other diets. The population of Ruminococcus albus and Ruminococcus flavefaciens was greater (p < .05) in goats fed 4% oil blend compared with those fed other diets. Diet had no effect (p> 0.05) on microbial N yield, microbial protein synthesis, efficiency of microbial protein synthesis and the intake, retention and excretion of N. Dietary supplementation of blend of palm oil and canola oil altered rumen microbial profile but did not affect nitrogen metabolism in goats.

Myofibrillar Protein, Lipid and Myoglobin Oxidation, Antioxidant Profile, Physicochemical and Sensory Properties ofCaprine longissimus thoracisduringPostmortemConditioning

The study examined the impact of refrigerated storage on antioxidant profile, oxidative changes in myoglobin, lipids and myofibrillar proteins and quality attributes of longissimus thoracis (LT) muscle from goats. Analyses were conducted on LT obtained from carcasses of sixteen Boer bucks on 0, 1, 5 and 10 days postmortem. Ageing had no effect on antioxidant enzyme activities. Concentration of tocopherols and total carotenoids decreased over storage. Myoglobin, metmyoglobin reducing activity, redness and shear force decreased while metmyoglobin content and drip loss increased over storage. Free thiol decreased while carbonyls and TBARS increased over storage. The relative density of myosin heavy chain, actin and troponin T and the concentration of n−6 and n−3 fatty acids were stable until day 5 but declined afterward. Tocopherol and carotenoid were correlated (P < 0.05) with oxidative changes. Ageing did not affect consumer preference for juiciness, flavor and overall acceptability. However, preference for tenderness increased over storage. Practical Applications Information on the impact of postmortem ageing on the antioxidant status of chevon in relation to its oxidative changes, physicochemical properties and shelf life is limited. This study demonstrates that although tenderness improved, quality loss during ageing of chevon is inexorable. The tocopherols and carotenoids are the primary drivers of postmortem oxidative stability of chevon. Antioxidant enzymes do not seem to play a significant role in the postmortem oxidative stability of chevon. The oxidative stability of longissimus thoracis in goats could be maintained for 5 days during chill storage. The current findings would provide useful information to consumers, retailers and meat industries on the merits and demerits of postmortem ageing of chevon and the factors to consider when longer ageing periods are desired or necessitated.

Rural Enterprise Development (RED) Through Innovative Goat Production Systems

The RED project which was implemented in four focal villages (with interventions) in the municipality of Guimba, and control site (no intervention) in Maragol, Science City of Munoz, both in the province of Nueva Ecija in the Philippines aimed to transform goat raising from subsistence farming into a viable livestock-based rural enterprise. Baseline data on biophysical, socioeconomic, institutional, infrastructure, goat production systems, marketing practices and previous technical performance were determined using structured questionnaires, key informants, secondary data and participatory rural appraisal (PRA)-focus group discussion (FGD). Farmer-partners are small hold livestock raisers who cultivate an average of 1.5 ha paddy-rice. Farmer’s skill and knowledge, attitude, skills, aspirations and social competence were enhanced using Crop Animal Systems Research Network (CASREN) modalities, capacity building, and S&T interventions. The adoption of technology mixes learned by farmer-partners that included upgrading, housing/stall feeding, strategic deworming, and intensified use of forage legumes improved goat productivity. Establishment of a community-based selection and breeding system through upgrading using Anglo-Nubian or Boer bucks in the focal sites produced bigger and heavier (p <0.05) kids at birth (2.30 kg), at weaning (13.87 kg), and at slaughter (22.63 kg). The heavier animals at slaughter provided a net benefit of PHP 1,046.40 per head to the farmer-partners. Moreover, animal holdings increased by 55.84% because of the improvement in the quality of their stocks. The pre-weaning mortality was dramatically reduced (p<0.05) from 30.57% to 14.40% while the conception rate was higher at 89.5 % in the focal sites. RED facilitated the empowerment of communities depending on goat for their livelihood, transforming traditional goat raising from subsistence farming to profitable enterprise. RED is not just about successful science-based goat raising, it is about building enterprises and generating opportunities to enrich the lives of farmers.

Relationship between the Corynebacterium pseudotuberculosis, phospholipase D inoculation and the fertility characteristics of crossbred Boer bucks

The aim of this study was to investigate the effects of Corynebacterium pseudotuberculosis and phospholipase D (PLD) on buck's fertility, specifically, testosterone concentration, scrotal circumference and semen quality. Crossbred Boer goats (n=13) aged 12–14 months were divided into three groups. The first group (n=3) was inoculated with one ml sterile phosphate buffer saline subcutaneously into subaxillary area as the control. The second group (n=5) was inoculated with live C. pseudotuberculosis 1×109 cfu subcutaneously into subaxillary area. The third group (n=5) was inoculated with PLD 1ml/20kg BW intravenously into jugular vein. Blood collection was done twice a week over a period of three months for testosterone analyses. Semen was collected using electro-ejaculator once every two weeks whilst scrotal circumference was measured once every three weeks. Semen volume, semen pH and sperm progressive motility, concentration, live/dead percentage and morphology were evaluated. Bucks were euthanized three months' post inoculation and histopathological examination of the testicles and epididymis was performed. The results showed seven folds' significant decrease (P<0.05) in testosterone concentration in both C. pseudotuberculosis and PLD inoculated groups compared to the control. Semen volume, percentage of dead/live and abnormal sperm morphology showed significant increase (P<0.05) in both inoculated groups compared to the control. The following sperm defects were observed; tapered sperm, decapitated and knobbed head, thickened midpiece, distal and proximal droplet, sterilizing tail and folded tail. Scrotal circumference, semen pH, semen wave pattern, sperm motility and concentration showed significant decrease (P<0.05) in both inoculated groups compared to the control. The testicles showed varied degrees of degeneration and necrosis with shrunken seminferous tubules. In conclusion, testosterone concentration, scrotal circumference and semen quality were negatively affected in both inoculated groups. Moreover, percentages of live/dead and abnormal sperm morphology were also increased. The results suggest that both C. pseudotuberculosis and PLD had detrimental effects on buck's fertility.

Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats

BackgroundThe functionality of myofibrillar proteins is a major factor influencing the quality attributes of muscle foods. Nonetheless, the relationships between muscle type and oxidative changes in chevon during ageing are meagrely elucidated. Postmortem changes in antioxidant status and physicochemical properties of glycolytic gluteus medius (GM) and oxidative infraspinatus (IS) muscles in goats were compared.MethodsTwenty Boer bucks (9–10 months old, body weight of 36.9 ± 0.725 kg) were slaughtered and the carcasses were subjected to chill storage (4 ± 0.5 °C). Analyses were conducted on GM and IS muscles sampled on 0, 1, 4 and 7 d postmortem.ResultsChill storage did not affect the antioxidant enzyme activities in both muscles. The IS had greater (P < 0.05) superoxide dismutase and catalase activities than GM. Carotenoid and tocopherol contents did not differ between muscles but decreased (P < 0.05) over storage. The IS had higher (P < 0.05) glycogen and ultimate pH and lower (P < 0.05) shear force and cooking loss than GM. The carbonyl content, % metmyoglobin, drip loss and TBARS increased (P < 0.05) while free thiol, metmyoglobin reducing activity (MRA), shear force and myoglobin decreased (P < 0.05) over storage. Muscle type had no effect (P > 0.05) on free thiol, MRA and TBARS. The GM had lower (P < 0.05) redness on d 0 and 1 than IS while the IS had greater carbonyl, % metmyoglobin and drip loss than GM on d 7. The reflective density of slow myosin heavy chain (MHC) was higher (P < 0.05) while the density of fast MHC and actin was lower (P < 0.05) in IS than GM. Regardless of muscle type, the density of MHC decreased (P < 0.05) while that of actin was stable over storage. Nonetheless, the degradation of fast and slow MHC was greater (P < 0.05) in IS than GM. Muscle type had no effect (P > 0.05) on consumer preference for flavour, juiciness and overall acceptability. However, IS had higher (P < 0.05) tenderness score than GM on d 1 and 4 postmortem. Intramuscular fat was higher (P < 0.05) in IS compared with GM. Fatty acid composition did not differ between the muscles. However, GM had lower (P < 0.05) n-6/n-3 ratio than IS. The n-3 and n-6 PUFA declined (P < 0.05) while the SFA increased (P < 0.05) over storage.ConclusionThe changes in myofibrillar proteins and physicochemical properties of goat meat during postmortem chill storage are muscle-dependent.

Influence of Diet and Postmortem Ageing on Oxidative Stability of Lipids, Myoglobin and Myofibrillar Proteins and Quality Attributes of Gluteus Medius Muscle in Goats.

This study appraised the effects of dietary blend of 80% canola oil and 20% palm oil and postmortem ageing on oxidative stability, fatty acids and quality attributes of gluteus medius (GM) muscle in goats. Twenty-four Boer bucks were randomly allotted to diet supplemented with 0, 4 and 8% oil blend, fed for 100 days and slaughtered, and the GM muscle was subjected to a 7 d chill storage (4±1°C). Diet had no effect (P> 0.05) on the colour, drip loss, thiobarbituric acid-reactive substances (TBARS) value, free thiol, carbonyl, myoglobin and metmyoglobin contents, metmyoglobin reducing activity (MRA), antioxidant enzyme activities and abundance of myosin heavy chain (MHC) and actin in the GM muscle in goats. The meat from goats fed 4 and 8% oil blend had higher (P< 0.05) concentration of α and γ-tocopherol and abundance of troponin T compared with that from the control goats. The GM muscle from the oil-supplemented goats had lower (P< 0.05) concentration of C16:0 and greater (P< 0.05) concentration of C18:1n-9, C18:3n-3 and C20:5n-3 compared with that from the control goats. Nonetheless, diet did not affect (P< 0.05) the total fatty acid in the GM muscle in goats. Regardless of the diet, the free thiol and myoglobin contents, concentration of tocopherol and total carotenoids, MHC and MRA in the GM muscle decreased (P< 0.05) while carbonyl content, TBARS, drip loss and metmyoglobin content increased over storage. Dietary blend of 80% canola oil and 20% palm oil beneficially altered tissue lipids without hampering the oxidative stability of chevon.

Relationship of extender and packaging system an the length of preservation and the quality of chilled semen of Boer goat

&lt;p class="abstrak2"&gt;The aim of this research was to compare the effectiveness of different extender (either Triladyl or Tris Egg Yolk extender) and different packaging method (pool and straw) of chilled semen an the length of preservation and the quality of chilled semen of Boer goat. Semen was collected using an artificial vagina from 3 two years old Boer bucks with body weight of 50-55 kg. It was evaluated under a microscope, then each was diluted either in Tris egg yolk extender (TEY) or Triladyl. Those diluted sperms were then packed either in pool or straw and preserved at 5⁰C refrigerator. Sperm motility, viability and membrane integrity of each group were evaluated every 24 h for up to 5 days. Results showed that sperm motility in Triladyl of pool packaging system up to 3 days was higher than straw packaging system or TEY in pool or straw packaging system which were 45.8%, 26.1%, 32.1% and 9.1%, respectively (P&amp;lt;0.05). Percentage of sperm membrane integrity showed the same pattern to Triladyl both in pool and straw packaging system which was higher than TEY group (75.2% and 77,2%; P&amp;lt;0.05). Sperm viability in Triladyl both in pool or straw packaging system decreased (P&amp;lt;0.05) after 3 days of preservation (77.1% and 76.2%) but TEY significanly decreased after 4 days of preservation either in pool or straw packaging system (73.2% and 58.0%; P&amp;lt;0.05). It was concluded that sperm quality decreased with increasing of the length of preservation while Triladyl extender in pool packaging system showed the best quality.&lt;/p&gt;&lt;strong&gt;Key Words: &lt;/strong&gt;Chilled Semen, Boer, Triladyl, Tris Egg Yolk, Straw

Fatty acid composition, cholesterol and antioxidant status of infraspinatus muscle, liver and kidney of goats fed blend of palm oil and canola oil

The study assessed the lipid profile, cholesterol and antioxidant status of infraspinatus muscle, kidney and liver from goats supplemented with increasing levels of blend of 20% palm oil and 80% canola oil. Twenty-four Boer bucks were randomly assigned to diets containing 0, 4 and 8% oil blend, fed for 100 d and slaughtered. The tissues were subjected to 7 d postmortem storage at 4 °C. Diet did not affect total lipid and cholesterol content in the tissues. The proportions of C14:0 in infraspinatus muscle and kidney, and C15:0 in all tissues were lower (p < 0.05) while the proportion of C18:3n:3 was greater in supplemented goats than the control goats. Proportion of C18:1n-9 in infraspinatus muscle was higher (p < 0.05) in goats fed oil blend than the control goats. The liver and the infraspinatus muscle of the control goats had higher C18:1 trans-10 but lower C18:1 trans-11 compared with those fed other diets. Diet had no effect on catalase, superoxide dismutase and glutathione peroxidase activities, and the concentration of γ and δ-tocopherol and lipid oxidation in all tissues. The concentrations of α-tocopherol and total carotenoid were greater in the tissues of oil-fed goats compared with the control goats. Regardless of tissue, the catalase and superoxide dismutase activities were stable throughout storage. Lipid oxidative stability, glutathione peroxidase activity, tocopherol and carotenoid contents in the tissues decreased (p < 0.05) as postmortem storage progressed. Dietary 20% palm oil and 80% canola oil blend modified the fatty acids in goat meat and offal without compromising their oxidative stability.

Effects of dietary oil blend on fatty acid composition, oxidative stability and physicochemical properties of Longissimus thoracis et lumborum muscle in goats.

This study examined the effects of dietary blend of 80% canola oil and 20% palm oil (BCPO) on the physicochemical properties, antioxidant status, oxidative stability and fatty acid composition of Longissimus thoracis et lumborum (LTL) muscle from goats during chill storage. Over a 14-week feeding trial, 24 Boer bucks were randomly assigned to and supplemented with diets containing 0, 4 or 8% BCPO on a dry matter basis, slaughtered and the LTL was subjected to a 7 day chill storage. Neither diet nor post mortem ageing influenced (P > 0.05) antioxidant enzyme activities, chemical composition and cholesterol. Diet had no effect on the carbonyl content, free thiol content, water-holding capacity, tenderness, pH and glycogen. Oil-supplemented goats had higher (P < 0.05) C18:1 trans-11, C18:3n-3 and C20:5n-3, carotenoid, tocopherol and redness, and lower thiobarbituric acid reactive substances values than the control goats. Post mortem ageing decreased (P < 0.05) shear force and oxidative stability of chevon. No significant (P > 0.05) changes were found in the proportion of individual fatty acids throughout storage. Total polyunsaturated fatty acids (PUFA) decreased while total saturated fatty acids increased as storage progressed. Dietary BCPO enhanced n-3 PUFA without compromising the quality attributes of chevon.

Changes of microbial spoilage, lipid-protein oxidation and physicochemical properties during post mortem refrigerated storage of goat meat.

Examined was the effect of post mortem refrigerated storage on microbial spoilage, lipid-protein oxidation and physicochemical traits of goat meat. Seven Boer bucks were slaughtered, eviscerated and aged for 24 h. The Longissimus lumborum (LL) and Semitendinosus (ST) muscles were excised and subjected to 13 days post mortem refrigerated storage. The pH, lipid and protein oxidation, tenderness, color and drip loss were determined in LL while microbiological analysis was performed on ST. Bacterial counts generally increased with increasing aging time and the limit for fresh meat was reached at day 14 post mortem. Significant differences were observed in malondialdehyde (MDA) content at day 7 of storage. The thiol concentration significantly reduced as aging time increased. The band intensities of myosin heavy chain (MHC) and troponin-T significantly decreased as storage progressed, while actin remained relatively stable. After 14 days of aging, tenderness showed significant improvement while muscle pH and drip loss reduced with increase in storage time. Samples aged for 14 days had higher lightness (P < 0.05) and lower (P < 0.05) yellowness and redness. Post mortem refrigerated storage influenced oxidative and microbial stability and physico-chemical properties of goat meat.

Serum fatty acids, biochemical indices and antioxidant status in goats fed canola oil and palm oil blend.

BackgroundDietary supplementation of unsaturated fats in ruminants, if not stabilized, can instigate oxidative stress which can have negative impact on production performance and enhance the susceptibility to various diseases. The current study examined the effect of dietary 80 % canola oil and 20 % palm oil blend (CPOB) on serum fatty acids, antioxidant profile and biochemical indices in goats. Thirty Boer bucks (4–5 months old; initial BW, 20.34 ± 0.77 kg) were randomly assigned to diets containing 0, 4 or 8 % CPOB and fed daily for a period of 90 days. Blood was sampled from the goats on 0, 30, 60 and 90 days of the trial and the serum was analyzed for fatty acids, cholesterol, glucose, total protein, antioxidants and lipid oxidation.ResultsNeither diet nor sampling time influenced serum TBARS value, catalase, glutathione peroxidase and superoxide dismutase activities, LDL cholesterol, VLDL cholesterol, triglycerides, glucose and total protein. Goats fed 4 and 8 % CPOB had higher (P < 0.05) total cholesterol and HDL cholesterol than the control goats on day 30, 60 and 90. The proportion of C15:0 decreased with increasing level of CPOB on day 30 and 60. Serum C18:1n-9 increased with increasing level of CPOB in diet on day 60. The proportion of C18:3n-3 and C22:5n-3 increased (P < 0.05), while the proportion of C18:2n-6 decreased (P < 0.05) with increase in the level of CPOB on day 60 and 90. Dietary CPOB did not affect serum total carotenoid and δ-tocopherol but did increase (P < 0.05) α and γ-tocopherol.ConclusionDietary canola oil and palm oil blend could be supplemented in diets without instigating oxidative stress in goats.

Effects of enriching goat meat with n − 3 polyunsaturated fatty acids on meat quality and stability

The effect of enriching goat meat with n−3 polyunsaturated fatty acids (PUFA) on meat quality, sensory evaluation, and lipid oxidative stability at different postmortem aging times was investigated. Samples from longissimus thoracis muscle of Boer bucks (n=24) fed diets containing 0% (control, L0), 10% (L10), or 20% (L20) whole linseed were collected for analysis. For meat quality and oxidative stability, sub samples were subjected to 1-day or 7-day postmortem aging period. Feeding linseed had successfully increased n−3 PUFA in goat meat. The increments in 18:3 n−3 were 682% and 975% for L10 and L20, respectively, compared to L0. The increments in total n−3 PUFA were 444% and 590%for L10 and L20, respectively, compared with L0. There was no effect on cooking loss, shear force and color at 1-day aging time. However, at 7-day of aging time the color was affected. Subjectively, the tenderness, juiciness and overall acceptability of L20 meat were rated better than those of L0 and L10. However, no differences were detected in color, flavor, and aroma attributes from sensory evaluation. Lipid oxidation was increased with increasing the contents of n−3 PUFA and postmortem aging time.

Quality Assurance of Cryopreserved Buck Semen by Assessing Structural and Functional Integrity of Spermatozoa

A total of 81 ejaculates collected from 4 Tellicherry and 2 Boer bucks were utilized to evaluate the structural and functional integrity of spermatozoa as a measure for quality assurance of cryopreserved buck semen. The semen samples were diluted with tris-egg yolk-glycerol based extender and frozen in straws. Only samples having 40% or more of post thaw motility (PTM) were regarded as “acceptable” samples for artificial insemination. The “acceptable” samples were further evaluated by hypo osmotic swelling test (HOST), sperm morphology and acrosome integrity assessment. Individual genotypes have shown significant variations (P < 0.01) for PTM. Significant variations (P < 0.01) were seen between bucks and between ejaculations of two Tellicherry bucks for hypo osmotic reacted spermatozoa. The differences in mean values for hypo osmotic reacted spermatozoa between I and II ejaculations of Tellicherry bucks were significant (P < 0.01). Significant variations (P < 0.05) were also observed for hypo osmotic reacted spermatozoa between I ejaculations of Tellicherry and Boer bucks. The variations in means of intact acrosome percent between I and II ejaculations of Tellicherry bucks was significant (P < 0.05). Besides post thaw motility, incorporation of structural and functional integrity tests like HOST and acrosome integrity in semen evaluation protocol add value to quality assurance of frozen buck semen.

Effects of Extenders on Fresh and Freezing Semen of Boer Goat

The aim of this study was to evaluate the effect of soybean lecithin and egg yolk on semen quality of Boer goats. Semen of the three Boer bucks was collected using artificial vagina. In the first experiment, the semen samples were preserved in liquid state and subsequently were diluted with Tris-fructose-citric acid (TFC), Tris-fructose-citric acid + 1.5% soybean lecithin (TFCSL) and Tris-fructose-citric acid + 2.5% egg yolk (TFCEY). In the second experiment, the semen samples were cryopreserved in various freezing media as the same treatment with experiment 1. The results from the first experiment indicated that sperm motility and viability in TFCEY were significantly greater than those of TFCSL (P<0.05). In the second experiment, there was no difference in post-thawing quality of semen in TFCEY and TFCSL. In conclusion, extender containing 1.5% soybean lecithin is an alternative for the preparation for freezing goat semen.

Impact of chill storage on antioxidant status, lipid and protein oxidation, color, drip loss and fatty acids of semimembranosus muscle in goats

ABSTRACTThe study examined the effect of refrigerated storage on antioxidant activities, lipid and protein oxidation, fatty acids (FAs), drip loss and color of semimembranosus (SM) muscle from goats. Samples of SM were obtained from carcasses of 15 Boer bucks (7 months old; body weight, 32.18 ± 0.81 kg) subjected to an 8 d storage at 4°C. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities were stable while carotenoid, tocopherol, water holding capacity and redness declined (P < 0.05) as storage progressed. Carbonyl content increased from 1.85 to 4.73 nmol/mg protein while thiol content reduced from 54.22 to 42.82 nmol/mg protein. The TBARS value increased from 0.2 to 0.8 mg MDA/kg. SDS-PAGE expression of myosin heavy chain (MHC) decreased (P < 0.05) from 72.45 to 49.82 density/mm2 while actin reduced (P > 0.05) from 14.00 to 13.08 density/mm2. The concentrations of n-3 and n-6 FA decreased while the saturated FA increased over storage. Correlations (P < 0.05) were found between antioxidant vitamins and quality indicators of chevon.

Influence of dietary canola oil and palm oil blend and refrigerated storage on fatty acids, myofibrillar proteins, chemical composition, antioxidant profile and quality attributes of semimembranosus muscle in goats.

BackgroundImproving the unsaturated fatty acid content of ruminant meat is essential due to the generally saturated nature of fatty acids in ruminant meat and the negative effects this can have on human health. Nonetheless, enhancing the unsaturated fatty acid content of ruminant meat can have adverse effects on the shelf life and quality attributes of the meat. This study assessed the effects of dietary 80 % canola oil and 20 % palm oil blend (CPOB) on fatty acid composition, antioxidants, oxidative spoilage, cholesterol and physicochemical properties of semimembranosus (SM) muscle from goats. Twenty four Boer bucks were randomly assigned to diets containing on dry matter basis 0, 4 and 8 % CPOB, fed for 100 d and slaughtered. The carcasses were subjected to a 7 d postmortem refrigerated storage. All analyses were conducted on the SM muscle.ResultsDiet had no effect (P > 0.05) on the concentration of free thiol and carbonyl and the band intensity of myosin heavy chain, actin and troponin T. The muscle glycogen, pH, water holding capacity, tenderness, glutathione peroxidase (GPX) activity, total carotenoid, δ-tocopherol, cholesterol and proximate composition did not differ (P > 0.05) between diets. The SM muscle from goats fed 4 and 8 % CPOB had lower (P < 0.05) concentration of C14:0 and C16:0 and higher (P < 0.05) concentration of C18:1 trans-11, C18:1ω-9, C18:3ω-3, C20:5ω-3 and C22:5ω-3 than the SM muscle from the control goats. Dietary CPOB increased (P < 0.05) the concentration of α and γ tocopherol and meat redness (a*) on d 1 and 4 postmortem. Regardless of diet, antioxidant vitamins, and shear force decreased (P < 0.05) while drip loss, lipid and protein oxidation increased (P < 0.05) as postmortem storage progressed.ConclusionResults evince that dietary CPOB can be used as a management tool to enhance the beneficial fatty acids and antioxidant contents of chevon without deleterious effects on its physicochemical properties and shelf life.

Effects of blend of canola oil and palm oil on nutrient intake and digestibility, growth performance, rumen fermentation and fatty acids in goats.

The study examined the effects of blend of 80% canola oil and 20% palm oil (BCPO) on nutrient intake and digestibility, growth performance, rumen fermentation and fatty acids (FA) in goats. Twenty-four Boer bucks were randomly assigned to diets containing 0, 4 and 8% BCPO on a dry matter basis, fed for 100 days and slaughtered. Diet did not affect feed efficiency, growth performance, intake and digestibility of all nutrients except ether extract. Intakes and digestibilities of ether extract, unsaturated fatty acids (FA) and total FA were higher (P < 0.05) while digestibility of C18:0 was lower (P < 0.05) in oil-fed goats than the control goats. Total volatile FA, acetate, butyrate, acetate/propionate ratio and methane decreased (P < 0.05) with increasing BCPO but propionate, NH3 -N and rumen pH did not differ between diets. Ruminal concentration of C18:0, n-3 FA and total FA increased (P < 0.05) while C12:0, C14:0, C15:0 and n-6 FA decreased with increasing BCPO. Analysis of the FA composition of Triceps brachii muscle showed that concentrations of C16:0, C14:0 and C18:2n-6 were lower (P < 0.05) while C18:1n-9, C18:3n-3 and C20:5n-3 were higher in oil-fed goats compared with control goats. Dietary BCPO altered muscle lipids without having detrimental effects on nutrient intake and digestibility and growth performance in goats.