Abstract
ABSTRACTThe present study aimed to investigate single-nucleotide polymorphisms (SNPs) of caprine FSHβ and caprine LHβ genes and to determine effects of the SNPs on fresh and post-thaw semen quality traits of Boer and Boer-crossbred bucks. The single-strand conformation polymorphism analysis and comparative sequencing revealed three SNPs in exon 3 of caprine follicle-stimulating hormone beta (FSHβ), including, three SNPs; 200A > G (FSHβ3-a), 226T > C (FSHβ3-b), and 237A > G (FSHβ3-c), while two of them (FSHβ3-a and FSHβ3-b) were novel. Furthermore, one SNP in exon 2 of luteinizing hormone beta (LHβ) (207T > C) (LHβ2) was detected. The associations of the four detected SNPs with quality traits of fresh and post-thaw semen were evaluated. Analyses of variance revealed significant association of the candidate genes with libido and semen quality traits. The three SNPs of FSHβ3 had significant effect on libido, progressive motility, and abnormality of fresh semen (P < .05), and on the motility, velocity, and viability traits of post-thaw semen (P < .05). LHβ2 polymorphism only showed association with sperm viability of post-thaw semen. The results of the present study suggest that the SNPs in caprine FSHβ and LHβ are associated with semen quality of male goats.
Highlights
Fertility of male goats is important in herd fertility and of importance in the goat industry
Interaction among gonadotropin-releasing hormone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone are essential for spermatogenesis (Barth et al 2008)
Two different primer pairs for caprine FSHβ gene were investigated for single-nucleotide polymorphisms (SNPs)
Summary
Fertility of male goats is important in herd fertility and of importance in the goat industry. With the development of molecular marker techniques and marker assistant selection in animal breeding, the candidate gene method may identify markers that can predict sperm quality traits in male goats. Giesecke et al (2010) elucidated that hormone and hormone receptors can be proper candidate genes for sperm quality traits due to their importance in male reproductive pathways. Spermatogenesis is controlled initially by the pituitary gland. Interaction among gonadotropin-releasing hormone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone are essential for spermatogenesis (Barth et al 2008). There are some single-nucleotide polymorphisms (SNPs) in FSH and LH genes, which cause a lack of or deficiency in FSH or LH hormones that cause defects in reproduction and fertility of men (Layman et al 2002; Lee et al 2003; Mafra et al 2010)
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