Body Fluid Loss Research Articles

Additive Diuretic Action of SGLT2 Inhibitor and Loop Diuretic Combination Avoids Body Fluid Loss by Promoting Vasopressin-Independent Compensatory Fluid Intake

Additive Diuretic Action of SGLT2 Inhibitor and Loop Diuretic Combination Avoids Body Fluid Loss by Promoting Vasopressin-Independent Compensatory Fluid Intake

Recommended homemade fluid utilization for the treatment of diarrhea and associated factors among children under five in sub-Saharan African countries: a multilevel analysis of the recent demographic and health survey

IntroductionDiarrhea is a common public health problem and the third leading cause of death in the world among children under the age of five years. An estimated 2 billion cases and 1.9 million deaths are recorded among children under the age of five years every year. It causes body fluid loss and electrolyte imbalance. Even though, early initiation of recommended homemade fluid is a simple and effective approach to prevent diarrhea-related complications and mortality of children, recommended homemade fluid utilization for the treatment of diarrhea is still low in sub-Saharan African countries. Therefore, this study aimed to assess the magnitude of recommended homemade fluid utilization for the treatment of diarrhea and associated factors among children under five in sub-Saharan African countries.MethodThe most recent Demographic and Health Survey dataset of 21 sub-Saharan African countries from 2015 to 2022 was used for data analysis. A total of 33,341 participants were included in this study as a weighted sample. Associated factors were determined using a multilevel mixed-effects logistic regression model. Significant factors in the multilevel mixed-effect logistic regression model were declared significant at p-values < 0.05. The adjusted odds ratio (AOR) and confidence interval (CI) were used to interpret the results.ResultThe overall recommended homemade fluid utilization for the treatment of diarrhea among children under five in sub-Saharan African countries was 19.08% (95% CI = 18.66, 19.51), which ranged from 4.34% in Burundi to 72.53% in South Africa. In the multivariable analysis, being an educated mother/caregiver (primary and secondary level) (AOR = 1.15, 95% CI: 1.04, 1.27) and (AOR = 1.30, 95% CI: 1.15, 1.1.47), the primary and secondary level of fathers education (AOR = 1.53, 95% CI: 1.37, 1.71) and (AOR = 1.41, 95% CI: 1.19, 1.1.68), having antenatal care follow-up (AOR = 1.16, 95% CI: 1.01, 1.33), having multiple children (AOR = 1.17, 95% CI: 1.07, 1.28), and being an urban dweller (AOR = 1.15, 95% CI: 1.04, 1.27) were factors associated with recommended homemade fluid utilization.ConclusionThe overall recommended homemade fluid utilization for the treatment of diarrhea was low. Individual and community-level variables were associated with recommended homemade fluid utilization for the treatment of diarrhea. Therefore, special consideration should be given to rural dwellers and caregivers who have three and below children. Furthermore, better to strengthen the antenatal care service, mother/caregiver education, and father’s education to enhance recommended homemade fluid utilization for the treatment of diarrhea.

Neuronally differentiated macula densa cells regulate tissue remodeling and regeneration in the kidney.

Tissue regeneration is limited in several organs, including the kidney, contributing to the high prevalence of kidney disease globally. However, evolutionary and physiological adaptive responses and the presence of renal progenitor cells suggest an existing remodeling capacity. This study uncovered endogenous tissue remodeling mechanisms in the kidney that were activated by the loss of body fluid and salt and regulated by a unique niche of a minority renal cell type called the macula densa (MD). Here, we identified neuronal differentiation features of MD cells that sense the local and systemic environment and secrete angiogenic, growth, and extracellular matrix remodeling factors, cytokines and chemokines, and control resident progenitor cells. Serial intravital imaging, MD nerve growth factor receptor and Wnt mouse models, and transcriptome analysis revealed cellular and molecular mechanisms of these MD functions. Human and therapeutic translation studies illustrated the clinical potential of MD factors, including CCN1, as a urinary biomarker and therapeutic target in chronic kidney disease. The concept that a neuronally differentiated key sensory and regulatory cell type responding to organ-specific physiological inputs controls local progenitors to remodel or repair tissues may be applicable to other organs and diverse tissue-regenerative therapeutic strategies.

Arterial Blood Gases and Acid-Base Regulation.

Disorders of acid-base status are common in the critically ill and prompt recognition is central to clinical decision making. The bicarbonate/carbon dioxide buffer system plays a pivotal role in maintaining acid-base homeostasis, and measurements of pH, PCO2, and HCO3 - are routinely used in the estimation of metabolic and respiratory disturbance severity. Hypoventilation and hyperventilation cause primary respiratory acidosis and primary respiratory alkalosis, respectively. Metabolic acidosis and metabolic alkalosis have numerous origins, that include alterations in acid or base intake, body fluid losses, abnormalities of intermediary metabolism, and renal, hepatic, and gastrointestinal dysfunction. The concept of the anion gap is used to categorize metabolic acidoses, and urine chloride excretion helps define metabolic alkaloses. Both the lungs and kidneys employ compensatory mechanisms to minimize changes in pH caused by various physiologic and disease disturbances. Treatment of acid-base disorders should focus primarily on correcting the underlying cause and the hemodynamic and electrolyte derangements that ensue. Specific therapies under certain conditions include renal replacement therapy, mechanical ventilation, respiratory stimulants or depressants, and inhibition of specific enzymes in intermediary metabolism disorders.

Results of studying the diabetic retinopathy course in potential kidney and pancreatic recipients while on hemodialysis

Introduction. Kidney and pancreas transplantation is a surgical method for the treatment of patients with diabetes mellitus and terminal diabetic nephropathy. While waiting for surgical treatment, potential recipients receive maintenance hemodialysis. Dialysis initiates the loss of body fluid, which in turn can affect the state of the intraocular structures. Aim. To study the effect of long-term hemodialysis therapy on ophthalmic parameters in patients with terminal diabetic nephropathy. Material and methods. Sixty patients (120 eyes) were examined: group A included 30 patients with end-stage renal failure as a result of diabetic nephropathy, group B included 30 people without systemic and ocular pathologies. The ophthalmological status of group A was assessed at the stage of planned preparation for renal replacement therapy, at 3 and 6 months after the initiation of dialysis. Ophthalmological examination consisted of the use of traditional and special diagnostic methods (microperimetry, photorecording of the fundus, optical coherence tomography angiography). Results. Within 6 months of hemodialysis courses, the following was recorded: a decrease in the thickness of the retina (Me: from 348.5 to 306.1 µm; p &lt;0.05) and choroid (Me: from 330.3 to 294.9 µm; p &lt;0.05), the improvement of retinal perfusion in eyes with diabetic macular edema (Me in the superficial capillary plexus: from 10.6 to 15.8% in the fovea, from 19.7 to 25.4% in the parafovea; in the deep capillary plexus: from 15.4 to 20.9% in the fovea, from 27.5 to 33.5% in the parafovea; p &lt;0.05), a decrease in choroidal hemoperfusion (Me in the layer of choriocapillaries: from 59.0 to 54.2% in the fovea, from 59.3 to 54.7% in the parafovea; in the deep layer of the choroids: from 55.5 to 50.7% in the fovea, from 55.3 to 50.7% in the parafovea; p&lt;0.05),an improvement in retinal photosensitivity (Me: from 16.7 to 20.3 dB in eyes with diabetic macular edema; from 21.1 to 24.2 dB in eyes without diabetic macular edema; p&lt;0.05) and increased visual acuity in eyes with macular edema (Me: 0.1 to 0.3; p&lt;0.05). Conclusions. Against the background of maintenance hemodialysis hemodialysis in patients with terminal diabetic nephropathy, along with a decrease in hyperazotemia, there is an improvement in architectonics, hemodynamics of the retina and visual functions.

Thermal stress, hydration, and salivary and respiratory stress markers in curling players performing a match in the cold.

Curling is a target-based team sport played in a cold environment. The type of stress curling players face during a curling match remains to be determined. In the present study, 16 Japanese curling players performed a practice curling match (six ends lasting 90 min), wherein the following variables were documented: core and skin temperatures, heart rate, thermal sensation and comfort, urine-specific gravity, body fluid loss, salivary cortisol, α-amylase activity, salivary secretory immunoglobulin A (SIgA), and fractionated exhaled nitric oxide (FeNO, a respiratory stress marker). Pre-match resting core temperature was 37.24 ± 0.31°C, which increased up to 37.73 ± 0.41°C during the match (p < 0.001). Facial skin temperatures decreased after the match (all p ≤ 0.015), whereas finger skin temperatures remained unchanged (p ≥ 0.375). Thermal discomfort increased following the match but thermal sensation remained unchanged. Following the match, players lost 0.29 ± 0.15 L body fluid (sweat, respiratory evaporation, and urine), which was nearly compensated by fluid ingestion of 0.22 ± 0.13 L (p = 0.119). Nevertheless, urine-specific gravity increased from 1.021 ± 0.010 to 1.024 ± 0.008 after the match (p = 0.012), with 31% and 50% players being dehydrated at pre- and post-match, respectively. Salivary cortisol decreased (p < 0.001) after the match without changes in salivary SIgA, α-amylase activity, and FeNO (all p ≥ 0.113). Therefore, during a curling match, the core temperature and thermal discomfort increase, whereas the face skin temperature decreases. Additionally, players may undergo dehydration before the match, which could be exacerbated after the match.

Tape‐Free, Digital Wearable Band for Exercise Sweat Rate Monitoring

AbstractMonitoring sweat secretion rate is essential for uncovering underlying physical conditions like hyperhidrosis, mental stress, and neural disorders. Often, flexible microfluidic sweat rate monitoring devices use tape as a means of attachment to the skin to tightly seal the collection area. While these single‐use, adhesive‐backed devices have lightweight and thin interfaces for mounting on the skin, their form factor complicates their potential integration with available commercial wearables, such as smartwatches. Here, a tape‐free device, consisting of a 3D‐printed sweat collector with a concave surface that is strapped onto the skin to form an effective seal, is presented. The materials, structure, and dimensions of the sweat collector are optimized for conformal device‐to‐skin contact and efficient capture of sweat. The collector is interfaced with a fluidic microchannel with embedded electrodes for continuous digital monitoring of sweat rate. Long‐term exercise‐induced local sweat rate from multiple body locations in both multi‐subject and longitudinal studies is measured, depicting the correlation between the measured sweat profile and total body fluid loss. The simple installation procedure and reusability of this tape‐free device make it a good candidate for integration with the band of a watch.

PS-BPB05-6: TOLVAPTAN INDUCES BODY FLUID LOSS AND ACTIVATION OF RENAL UREA-DRIVEN WATER CONSERVATION IN NORMAL RATS

Objective: We recently reported that body fluid loss models such as high salt-induced natriuresis, impaired urine concentration ability via kidney injury, and enhanced transepidermal water loss due to skin barrier dysfunction exhibit initial water loss and subsequent activation of the water conservation system in multiple organs. This activation of the water conservation system restores or even increases water content at the tissue level, which may be involved in abnormal body fluid distribution in various disease models. The water conservation system consists of renal urea recycling, the energy-intensive nature of hepato-muscular ureagenesis, and suppression of cardiovascular energy expenditure to save energy for ureagenesis. However, it is not clarified whether pharmacological fluid loss by diuretics activates the water conservation system. In this study, we examined the effects of water diuresis by tolvaptan, a selective vasopressin V2 receptor antagonist, on the water conservation system in normal rats. Design and method: 10-week-old male Sprague Dawley rats were fed with a control diet or a diet containing 0.1% tolvaptan. We measured water intake and urine volume for two weeks and also evaluated time-dependent changes in renal urea recycling and hepato-muscular ureagenesis in these rats. In addition, we also evaluated the effects of tolvaptan on blood pressure, heart rate, and renal sympathetic nerve activity by a radiotelemetry system in separate animals. Results: Tolvaptan markedly increased urine volume up to 126.7 ± 25.9 ml on day-1, which gradually decreased as much as one-third (48.1 ± 18.0 ml) on day-7. Tolvaptan significantly decreased body water content and increased plasma albumin concentration, an indicator of oncotic pressure, on day-1, suggesting that tolvaptan decreased body fluid and plasma volume on day-1. These changes in total body water content and plasma albumin concentration were restored on day-7. The restoration of body fluid on day-7 was associated with an increase in urea transporter A1-associated renal urea recycling. On the other hand, tolvaptan did not alter hepato-muscular ureagenesis or cardiovascular energy expenditure throughout the treatment. Conclusions: Tolvaptan induces initial body fluid loss by water diuresis and subsequent activation of renal urea recycling to restore the water loss. Activation of the water conservation system in addition to a rise in urine volume may be involved in the unknown distinct effect of the diuretics.

Assessment of Knowledge and Usage of Oral Rehydration Therapy in Management of Childhood Diarrhea among Mothers of Kambaza town, Kebbi State, Nigeria

Diarrhea is one of the major causes of infant and young children morbidity and mortality globally. It is characterized by passing of more than 3 watery stools within 24hr which is accompanied by loss of body fluids and electrolytes leading dehydration and death if not treated. Therefore, this study was aimed in assessing knowledge and use of Oral Rehydration Therapy (ORT) in the management of childhood diarrhea among mothers. A cross sectional descriptive study was conducted among 103 mothers living in Kambaza metropolis using a questionnaire for quantitative data collection. The data were analyzed using Statistical Package for Social Sciences (SPSS), version 20 and p-value &lt;0.05 was considered statistically significant. The results showed that a significant proportion of respondents were below the age of 36 years (25-35years) with the mean age of 30±0.327 years and most of them (92.2%) were married. 81.6% of the respondents heard knowledge about ORT and majority of them (82.5%) indicated they know to prepare ORS/SSS. The use of ORT in the management of childhood diarrhea was also found to be high (82.5%) among the mothers. A significant portion of the mothers (95.1%) indicated already made ORS sachet was more effective in treating diarrhea than home-made SSS. There is no significant relationship between maternal level education and use of ORT in the management child diarrhea (p=0.476). However, a significant relationship exit between maternal knowledge on ORT preparation and its usage for management of childhood diarrhea (p=0.037). This study recorded high knowledge and use of ORT for management of childhood diarrhea among mothers of under-five children. Therefore, it recommended that maternal knowledge on use of ORT and its preparation should be encourage.

A Spiking Neural Network Based on Retinal Ganglion Cells for Automatic Burn Image Segmentation.

Burn is a common traumatic disease. After severe burn injury, the human body will increase catabolism, and burn wounds lead to a large amount of body fluid loss, with a high mortality rate. Therefore, in the early treatment for burn patients, it is essential to calculate the patient's water requirement based on the percentage of the burn wound area in the total body surface area (TBSA%). However, burn wounds are so complex that there is observer variability by the clinicians, making it challenging to locate the burn wounds accurately. Therefore, an objective, accurate location method of burn wounds is very necessary and meaningful. Convolutional neural networks (CNNs) provide feasible means for this requirement. However, although the CNNs continue to improve the accuracy in the semantic segmentation task, they are often limited by the computing resources of edge hardware. For this purpose, a lightweight burn wounds segmentation model is required. In our work, we constructed a burn image dataset and proposed a U-type spiking neural networks (SNNs) based on retinal ganglion cells (RGC) for segmenting burn and non-burn areas. Moreover, a module with cross-layer skip concatenation structure was introduced. Experimental results showed that the pixel accuracy of the proposed reached 92.89%, and our network parameter only needed 16.6 Mbytes. The results showed our model achieved remarkable accuracy while achieving edge hardware affinity.

Postpartum Hypernatremia with Extrapontine Rhabdomyelinolysis: A Case Report.

Hypernatremia (serum sodium>160 meq/L) present with diverse neurological manifestations, ranging from flaccid paralysis to impaired cognition, encephalopathy, and even deep coma. Osmotic demyelination refers to changes in brain cells because of an acute change in plasma osmolality. It is further divided into two types, i.e., central pontine myelinolysis (CPM) and extrapontine myelinolysis (EPM). Patients with EPM, besides spasticity, may also present with other movement disorders such as catatonia, parkinsonism, and dystonia. We present a case of a postpartum woman bought to the emergency department by her relatives in an unconscious state. In view of poor sensorium (Glasgow coma scale < 7), she was intubated and received mechanical ventilatory support. On admission, computed tomography ofthebrain was normal, and the patient was transferred to the intensive care unit (ICU) for further management. The preliminary work-up in the ICU showed hypernatremia (serum sodium of 182 mEq/L) with hyper-osmolality (359 mOsm/kgH2O). She was managed as per the ICU protocol for hypernatremia. During her ICU stay, her sensorium improved, but she developed flaccid paralysis and was quadriplegic. Thus, a tracheostomy was performed, and she was weaned from the ventilator. After ventilator weaning, she was transferred to the ward for further rehabilitation. During rehabilitation, the patient was able to sit and takefoodorally.To date, only a few cases are reported in postpartum women with acute severe hypernatremia caused by high-grade fever and loss of body fluids leading to extra-pontine demyelination and flaccid paralysis. This case highlightsthat prompt recognition and appropriate intervention can improve the outcomes in these patients.

Effect Of The Combination Of Aloe Vera And Palm Sugar On The Improvement Of Diabetic Ulcers In Patients With Type 2 Diabetes Mellitus

Background: Diabetic ulcer treatment with aloe vera and palm sugar is one of the treatment measures to prevent infection in diabetic ulcers. Aloe vera contains auxin, gibber-relin, anthraquinone, and vitamins A, C, and E as an anti-inflammatory, antipyretic, antioxidant, antiseptic, antimicrobial, as well as antiviral. Aloe vera can diffuse properly and can withstand the loss of body fluids from the surface of the skin so that moisture is maintained, while palm sugar contains vitamin C, Riboflavin, vitamin A, and ascorbic acid as antibiotics and improves the tissue work system which can weaken and kill bacteria that cause infections in diabetic ulcers. Purpose: The purpose of this study was to determine the effect of the combination of aloe vera and palm sugar on the improvement of diabetic ulcers in patients with type 2 diabetes mellitus. Method: One group pretest and posttest design research design in one subject group with a total sample of 8 people, sampling technique using consecutive sampling, the research was carried out in August 2022 at the Wanaraja health center, Garut Regency Results: Based on the results of the study, it is known that the highest activity that occurs in Macaca fascicularis at Sangeh Tourism Object is in the behavior of moves (moving) with a percentage of 17%, and Grooming behavior (Looking for lice) by 15%, then for other activities such as rest (rest) by 14 %, mating 8%, feeding 13%, social 14%, agonistic 13%, for behavior with the lowest percentage of sleep (sleep) of 6%. Conclusion: Conclusion of the study, the combination of aloe vera and palm sugar affects the improvement of diabetic ulcers in patients with type 2 diabetes mellitus

Ergogenic benefits of carbohydrate mouth rinsing on endurance exercise performance

Carbohydrate ingestion during exercise has been extensively investigated to enhance exercise performance, particularly during prolonged exercise (&gt;2 h) when endogenous carbohydrate is limited. The pertinent benefit of carbohydrate ingestion is that it delays the depletion of vital energy source for exercise, hence delaying fatigue. Athletes have often been advised to drink or rehydrate (replace body fluid loss) to ensure sufficient energy source and to avoid dehydration during exercise Nevertheless, the ability for athletes to rehydrate in a real race setting, maybe challenging due to fluid availability and the nature of the event, such as during a running event. As an alternative to drinking, carbohydrate mouth rinsing has resulted in enhanced prolonged exercise performance. The underlying mechanism responsible for this improvement has been associated with the activation of the oral receptor that is related to reward and behavioural centre of the brain that contributed to enhancing exercise performance. Numerous studies have examined factors that influence the effectiveness of carbohydrate mouth rinsing. While these studies have presented convincing evidence to support their hypothesis, future studies are required to provide new insight into the effectiveness of carbohydrate mouth rinsing on exercise performance. These questions include how the environmental condition and dehydration level may influence the effectiveness of carbohydrate mouth rinsing on endurance exercise performance.

Effects of Mild Body Fluid Loss Because of Overnight Fasting on Dynamic Cerebral Autoregulation in Young Male Volunteers.

Ogawa, Yojiro DDS, PhD*; Konishi, Toru MD, PhD*,†; Kurazumi, Takuya MD, PhD*,‡,§; Takko, Chiharu DDS*; Kato, Tomokazu MD, PhD*; Iwasaki, Ken-ichi MD, PhD* Author Information

Glomerular Filtration Rate Reserve During Mild Passive Heat Stress Does Not Differ Between Younger Men and Women

PurposeThe incidence of acute kidney injury during heat waves is higher in young women compared to young men. Acute kidney injury is clinically defined as an abrupt reduction in glomerular filtration rate (GFR). The magnitude of reduction in GFR during acute kidney injury is reflective of the availability of functional nephrons. Functional nephron mass is lower in women compared to men. Therefore, women may be at a greater risk of AKI during heat waves due to a reduced ability to maintain GFR. In a heterogenous group of men and women, we have recently demonstrated that GFR reserve, which is defined as the capacity to increase GFR in response to an increase in functional demand (e.g., oral protein load), is reduced with passive heat stress. This study tested the hypothesis that GFR reserve is lower in women compared to men during passive heat stress.MethodsFollowing 2 hours of passive heat stress (water perfused suit), healthy young men (n=13) and women (n=12, eumenorrheic, tested in the early follicular phase) ingested a whey protein shake (1.2 g of protein/kg of body weight) to test GFR reserve. Serum (sCr) and urinary (uCr) creatinine and urine flow rate were measured pre‐protein, and 75‐min and 150‐min post‐protein ingestion. GFR was estimated from creatinine clearance (CrCl = uCr x urine flow rate ÷ sCr). Data are presented as mean ± SD.ResultsThe increase in core temperature pre‐protein (men: 0.7 ± 0.2, women: 0.8 ± 0.2°C, p=0.829) and total body fluid loss (men: ‐2.9 ± 0.7, women: ‐2.9 ± 0.8%, p=0.926) did not differ between men and women. sCr did not differ between men (pre‐protein: 1.1 ± 0.2 mg/dL) and women (pre‐protein 1.0 ± 0.1 mg/dL) at any time (p=0.097) and did not change from pre‐ following protein ingestion (p=0.107). uCr was elevated above pre‐protein levels (men: 94.4 ± 78.4, women: 40.0 ± 14.0 mg/dL) in both men and women at 75‐ and 150‐ min post‐protein (p≤0.011) but did not differ between groups at any time (p≥0.105). Urine flow rate was reduced post‐protein (p&lt;0.001), but there were no differences between men and women (p=0.331). Pre‐protein CrCl was higher in men (130 ± 32 mL/min) versus women (89 ± 12 mL/min, p=0.005), which was maintained throughout the post‐protein period (p≤0.005). CrCl increased by 15 ± 19 mL/min from pre‐ to 150‐min post‐protein in men (p=0.022) but did not differ from pre‐protein in women (change at 150‐min post‐protein: +9 ± 21 mL/min, p=0.568). However, the peak increase in CrCl post‐protein, independent of time, did not differ between men (+19 ± 19 mL/min) and women (+11 ± 19 mL/min, p=0.193).ConclusionDuring mild passive heat stress, the magnitude of increase in CrCl following an oral protein load did not differ between young healthy men and eumenorrheic women tested in the early follicular phase of the menstrual cycle. Thus, biological sex differences in GFR reserve during heat exposure do not likely contribute to the increased risk of acute kidney injury during heat waves in young women when estrogen is at its lowest levels. Whether GFR reserve during passive heat stress is modified by the menstrual cycle phase requires further investigation.

Tolvaptan induces body fluid loss and subsequent water conservation in normal rats

We have recently reported that the urea osmolyte-associated water conservation system is activated in fluid loss models such as high salt-induced natriuresis, renal injury-induced impaired renal concentrating ability, or skin barrier dysfunction-induced transepidermal water loss. The system consists of the interaction of multiple organs including renal urea recycling, hepato-muscular ureagenesis, and suppression of cardiovascular energy expenditure. Here, we determined the effect of pharmacological fluid loss induced by tolvaptan, a selective vasopressin V2 receptor antagonist, on water conservation. We evaluated the water conservation system in rats that consumed a control diet or a diet containing 0.1% tolvaptan. Tolvaptan increased urine volume on day 1, but this renal water loss then gradually decreased. Body water and osmolyte content were decreased by tolvaptan on day 1 but had normalized by day 7. Tolvaptan induced fluid loss on day 1, and the following restoration of body fluid on day 7 was associated with an increase in urea transporter A1-associated renal urea recycling. Tolvaptan did not affect hepato-muscular ureagenesis on day 1 and day 7, or cardiovascular energy expenditure during treatment. Thus, tolvaptan-induced fluid loss leads to activation of the water conservation system via renal urea recycling.

Fumigation of three major soil pests (Agriotes lineatus, Diabrotica virgifera virgifera, Phyllopertha horticola) with 3-octanone and 1-octen-3-ol enantiomers

ABSTRACT New pest management solutions are needed to control soil invertebrates (insects, nematodes, mollusks) in order to implement the goals of the European Green Deal. Natural volatile organic compounds (VOCs) such as 1-octen-3-ol and 3-octanone, emitted by the entomopathogenic fungus Metarhizium brunneum could be part of the solution. Three major crop pests, Agriotes lineatus (wireworm), Diabrotica virgifera virgifera (corn rootworm) and Phyllopertha horticola (garden chafer), were susceptible to fumigation with 1-octen-3-ol and 3-octanone. The toxicity of the VOCs was tested in soil-free arenas and in soils which differed in moisture content and porosity. The mortality rates were dependent on the interaction between the VOC treatment, dose and pest species. The insects differed in their sensitivity to these VOCs. A dose of 1.25 µl of 1-octen-3-ol applied in a closed glass tube was sufficient to kill D. v. virgifera and P. horticola in soil trials whereas 5 µl was needed to kill A. lineatus. The highest dose (20 µl) was highly toxic to all insects. Soil moisture content slightly influenced mortality rates whereas porosity had no obvious impact. The mode of action of the VOCs is unknown but the compounds are likely to cause tissue damage and loss of body fluids. This may explain the shrivelled appearance of corn rootworm and garden chafer and melanisation in wireworm. Both 1-octen-3-ol and 3-octanone show promise as biofumigants.

Impact of running race in warm weather on hematological and biochemical parameters.

Exercise is highly essential for a healthy life. The athletes drink water to restore the body fluid volume and salt ingredients during the exercise. It is postulated that the blood parameters of athletes would greatly be affected if they run more than 10 km in controlled dehydration (without taking liquid) in a hot environment. This study aims to investigate the acute hematological changes in Saudi male athletes engaged in sports activities in a hot climate without taking fluid. In this cross-sectional study, the participants were adult Saudi athletes (n=12) who ran for a 10 km distance during the daytime when the temperature was ≥35°C. Blood samples were collected before and after the race and analyzed by standard methods for hematocrit, plasma volume, hemoglobin, total white cells count, red cells count, and blood lactate was examined. The mean age of athletes was 22.83 years; their mean height was 173.8 cm, and the mean weight was 56.1 kg. The average body fluid loss during the race was 1.88 ± 0.70 L. The white blood cells, hematocrit, hemoglobin levels were increased significantly at the end of the running event (p < 0.05). However, values of red blood cells and insulin were decreased. Exercise, mainly the running race in hot weather conditions, has a significantly increasing impact on athletes' hematological and biochemical parameters. The findings have an important message for the sports trainers and coaches to prepare prospective professional athletes and improve their performance for national and international sports events.

Effects of hypohydration and fluid balance in athletes’ cognitive performance: a systematic review

Background: The effects of progressive body fluid loss on athletic and cognitive performance are known to result from exposure to environmental heat stress, morphologic factors, and limited fluid replenishment. Athletes need to restore lost body water. However, athletes may fail to maintain euhydration during exercise. This systematic review investigated hypohydration and fluid balance effects on an athlete’s cognitive function.&#x0D; Methods: The PubMed, Sports Discuss, and Ebsco databases were searched for studies reporting on hypohydration, fluid balance and heat on cognitive performance in sport. Multiple phrases including hydration, dehydration, fluid balance, mood, cognition, vigilance, decision making, and brain were explored. Participants in the studies did either receive fluid or did not receive fluid during exercise.&#x0D; Results: Twenty-four trials (n=493 participants) from 24 articles met the inclusion criteria. Significant hypohydration, &gt;2% body mass loss was reported consistently in 16 publications. Five articles where hypohydration was associated with heat stress and limited fluid intake (3-5% body mass loss) impaired cognitive performance. Mood disturbance, fatigue, and ratings of perceived exertion constantly complemented hypohydration impairment on cognition.&#x0D; Conclusion: Findings show that hypohydration impairs cognitive performance and mood at higher levels of 3-5% body mass loss. However, sport-specific cognitive protocols of accessing hypohydration and fluid balance in individual and team sports remain equivocal.&#x0D; Keywords: Hypohydration; cognition; mood; fluid replenishment.

Nephrectomy in a Dog infected with Dioctophyma renale - Mato Grosso do Sul, Brazil

Background: Dioctophymosis is caused by Dioctophyma renale, a parasite known as the giant kidney worm, that can parasitize the kidneys of domestic and wild animals. There are also reports of its occurrence in humans, thus revealing its zoonotic potential. In most cases, parasitized animals are asymptomatic. This parasite can cause atrophy or destroy the renal parenchyma, although ectopic locations may occur. The diagnosis is made through ultrasonography, based on the presence of eggs in the urine, visualization of the parasite, or during necropsy. Therefore, the aim of this work was to report the case of a young dog infected with D. renale and subjected to nephrectomy in the state of Mato Grosso do Sul, Brazil.Case: A 6-month-old bitch with a clinical suspicion of hydronephrosis in the right kidney was referred to the Veterinary Hospital of Anhanguera-Uniderp University in the city of Campo Grande, MS. A physical examination of the patient revealed an alteration in the urinary system. An abdominal ultrasound, urinalysis, complete blood count (CBC) tests and biochemical profile were ordered. The erythrogram indicated erythrocytosis resulting from dehydration and loss of body fluids, while enzyme levels (creatinine, urea, alanine aminotransferase [ALT] and albumin) were within normal limits. The abdominal ultrasound showed the presence of a cylindrical and rounded structure characteristic of a nematode and in the right kidney, and loss of renal parenchyma typical of D. renale infection. A urinalysis then revealed the presence of helminth eggs, confirming the diagnosis. The owner was informed about the need for nephrectomy of the affected right kidney, which showed destruction of the renal parenchyma. One adult female and one adult male parasite were removed from inside the kidney, measuring approximately 50 cm and 35 cm in length. The patient was successfully treated, kept in hospital for observation, and returned two weeks later for reassessment of her renal function and removal of stitches. Discussion: Dioctophymosis is often diagnosed based on ultrasound and urine tests. These tests proved sufficient to diagnose parasitism by D. renale. However, the infection is usually discovered during necropsy. D. renale is popularly known as the giant kidney worm, as it can reach up to 100 cm in length. In the present case, the female parasite was 50 cm long and the male was 35 cm. The patient presented parasitism very young, at just 6 months of age. The parasitic infection of the animal was attributed to the ingestion of water or food contaminated with an intermediate host, the aquatic annelid Lumbriculus variegatus. It is suggested that the ingestion of food or water contaminated with the infective stage of the parasite may have occurred at 2 months of age or younger, since the prepatent period is approximately 6 months. The parasite was found only in the patient’s right kidney. Hydronephrosis was reported in the patient and was caused by obstruction of the internal urethral ostium by the adult nematode. In this case, the recommended surgical treatment was nephrectomy, to which the patient was subjected, leading to successful recovery. This case occurred in the state of Mato Grosso do Sul, where there are no records of parasitism by D. renale in domestic dogs, unlike other states in Brazil. We therefore emphasize the importance of new studies on D. renale, given the lack of clear records describing the parasite’s epidemiological data, biological cycle and diagnosis, which may hinder the prevention and control of this zoonotic disease.Keywords: canine dioctophymosis, helminth, nematoid, giant kidney worm, hydronephrosis.Descritores: dioctofimose canina, helminto, nematoide, verme gigante renal, hidronefrose.