<i>Aim:</i> This study evaluated profile of health related physical fitness of Academic and Non-academic staff of Federal University Dutsin-Ma, Nigeria. Design of the study: An ex-post facto research design was used for this study. <i>Methods:</i> The population for the study was four hundred and seventy-one (471) staff that came out for the study within three months. The sample was made up of 174 academics and 297 Non-academic staff which were purposively selected that participated in the study. The components of health-related physical fitness assessed were; cardio-respiratory fitness, flexibility, muscular endurance, muscular strength and body composition (optimal body fat levels) using appropriate field tests. <i>Results:</i> The result of the study shows that; both the academic and Non-academic staff do not show reasonable level of physical fitness in their body composition, muscular endurance, flexibility and muscular strength. Only the cardio respiratory fitness of participants was good. <i>Conclusion:</i> The study concluded that, both the academic and Non-academic staff of Federal University Dutsin-ma do not possess high profile of physical fitness with emphasis on their body composition, muscular endurance, flexibility and muscular strength which are of the necessity for quality living. It was also found that the level of actual involvement in physical fitness activities was very low. <i>Recommendations:</i> It was recommended that; there is the need for academic and Non-academic staff to be motivated by the University authority by making a policy on participation in physical activity during leisure time in order to reduce incidences of hypokinetic diseases amongst them; more indoor and outdoor facilities to be built to accommodate staffs to participate in sporting activities.