High-intensity Interval Training Improves Inflammatory Mediators in Obese Women: Based on the Study of the UCP2 Ala55Val Gene

Abstract

Background: Increased inflammatory mediators in obesity are associated with metabolic syndrome. Exercise is an effective effort to reduce the incidence of obesity. The High-Intensity Interval Training (HIIT) program is an exercise which include combination of high-intensity exercise and rest periods. The decrease in body fat levels due to physical training will further affect inflammatory mediators such as IL6 and TNFα. Besides training factor, genetic also play a role on obesity. One of the genes that influence obesity is the UCP2 Ala55Val gene.
 Objectives: This research aims to see the effect of HIIT on the levels of inflammatory mediators in obese patients based on the study of the Ala55Val UCP2 gene.
 Methods: This study was a Quasi-Experimental Pre and Post Design Without Control Group. Thirty obese women (BMI≥25 kg/m2) were given High-Intensity Interval Training (HIIT) as an intervention by comparing the data before and after the intervention. The training intervention was conducted for 12 weeks, consisting of two weeks of adaptation and ten weeks of HIIT intervention. The body weight, BMI and inflammatory mediators (TNFα and IL 6) before and after the intervention were analyzed using the Dependent T-Test and Wilcoxon Test as a nonparametric test. Independent T-Test and Mann Whitney test used to determine the effect of the UCP2 Ala55Val gene on changes in body weight, BMI and the inflammatory mediator. The test results were considered significantly different if p<0.05.
 Results: Bivariate analysis using Dependent T-Test showed that HIIT significantly improved Body Weight, BMI and IL6 with p=0.0001. Wilcoxon Test showed that HIIT significantly improved TNFα with p=0.0001. Independent T-Test showed no difference in body weight (p=0.719), BMI (p=0.663) and TNFα (p=0.264) improvement in the two genotypes of the UCP2 Ala55Val gene. Mann Whitney Test showed no difference in IL6 (p=0.288) improvement in the two genotypes of the UCP2 Ala55Val gene.
 Conclusion: The research concluded that the 12-week HIIT interventions improved inflammatory mediators by reducing IL6 and TNFα in obese women. There was no effect of genetic variation on the response to training intervention.