AbstractCatch rates of Yellow Perch Perca flavescens in standard gill‐net surveys conducted by the Minnesota Department of Natural Resources have declined since 1970, but it is unclear whether this trend was due to reduced abundance or to changes in size structure affecting catchability. Because the minimum capture length of standard gill nets may limit effective sampling of some populations, the objectives of this study were to determine if length‐based metrics could be used to characterize populations and their susceptibility to standard gill nets, if these metrics were correlated with a suite of abiotic and biotic variables, and if temporal trends in these metrics were evident. Data were collected from a broad size distribution of Yellow Perch sampled with standard gill nets, boat electrofishing, and small‐mesh gill nets in 17 lakes. Estimated length at 50% maturity (L50) for females varied up to 100 mm across lakes, with values as small as 70 mm documented in some populations. Female L50 from supplemental gears was significantly positively correlated with the average length of the 15 largest fish (Lmax) from all gears, and both of these metrics were positively correlated with standard gill‐net catch. Exploratory analysis of female L50 indicated that this metric was positively correlated with latitude and lake size and negatively correlated with water clarity. Additionally, a statewide analysis of Lmax conducted with standard gill‐net data from over 1,000 lakes found significant declines during the past 25 years, indicating that populations in Minnesota have likely shifted towards a smaller size structure, reducing catchability in standard gill nets. These results indicate that standard gill nets were ineffective for sampling some Yellow Perch populations and that length‐based metrics such as L50 and Lmax can be used to infer catchability in standard gear and to monitor populations through time.