Blood Plasma Of Children Research Articles

The application of an improved gas-liquid chromatographic method for the determination of the long chain non-esterified fatty acid pattern of blood plasma in children

An accurate, practicable and fast method for the quantitative determination of the non-esterified fatty acid (NEFA) pattern in plasma is described. After extraction, thin-layer chromatography and methylation, the NEF As are quantitated by injection on a gas Chromatographie column. A precise method with a mean recoveory of 96% and a coefficient variation of the total NEFA concentration between 4 and 5% has been developed by the addition of the internal standard before the extraction, the deduction of a blank value and the use of constant quantities of silicagel, taken from the thin-layer plates. The height × retention time of each methyl ester peak is calculated after injection on a free fatty acid phase (FFAP) column. The total NEFA concentration and the plasma NEFA pattern of Belgian schoolboys (15 to 17 years old) have been determined.

Variability of actual and potential fibrin-stabilizing factor (FSF) activity of blood plasma in healthy children.

Actual and potential fibrin-stabilizing factor (FSF) activity in the blood plasma of children in various are groups and in adults was estimated. Differences in blood plasma activities regarding desmofibrin formation were revealed, which depended upon the changes in enzyme concentration varying with age and upon the changes in thiol group concentration.

The Free Amino Acids in Blood Plasma of Children with Kwashiorkor and Marasmus

The Free Amino Acids in Blood Plasma of Children with Kwashiorkor and Marasmus

Electrophoretical Study on Bloodplasma of healthy Children

Electrophoretic analyses on blood plasma of children are so rare in our country that we studied the plasma protein analysis on 22 healthy children, 14 cases in 6 years (group A) and 8 yases in 2-4 years old (group B), with Tiselius' apparatus using Schlieren-diagonal method (Type HT-B Hitachi made).Pooled plasma were dialyzed for 16-20 hours, before electrophoresis, against 1/20M phosphate buffer.Experimental conditions were as follows; electric voltage 90-140V., electric current 9-10mA., time of electrophoresis 40-80 minutes, temperature at 16°C., Diagonal-Slit angle 25°.The results are shown in the next Table.In comparison with those values of the adult which were reported by many authors, 1) total protein showed no remarkable difference, 2) alubumin some decrease, and3) globulin no remarkable difference.3) globulin no remarkable difference.4) α-Globulin was not able to be compared with that of the adult, because there wag significant difference between group A and B. This may well be due to difference of time of electrophoresis.5) β-Globulin showed no remarkable differnce, 6) γ-globulin some increase, and7) fibrinogen no remarkable difference.

SERUM CHOLESTEROL VALUES FOR CHILDREN

The purpose of this report is a statistical evaluation of the free and total cholesterol values found for three groups of patients at the Seaside Hospital from June to September 1935. These patients, ranging in age from the neonatal period to 12 years, were about equally divided between the sexes. The pathologic conditions represented were (1) infection of tonsils and adenoids, (2) rheumatic fever, including rheumatic carditis, and (3) miscellaneous acute and chronic infections, including osteomyelitis, pleurisy, rickets, diabetes, acute glomerulonephritis, asthma, residual poliomyelitis, malnutrition, eczema, pylorospasm, torticollis and bronchiectasis. Little work has been done in the determination of values for free and combined cholesterol in the blood of children. Recently Sperry1reported free and total cholesterol values for heparinized blood plasma of children in the neonatal period. The range was from 25 to 61 mg. and from 71 to 190 mg. per hundred cubic centimeters for free and