Blood Hemolysates Research Articles

Influence of Ajuga turkestanica and Asparagus racemosus Phytoextracts on the Effectiveness of Aerobic–Anaerobic Training Regime

In this work, we study the effect of Asparagus racemosus (EAR) and Ajuga turkestanica (EAT) dry extracts at a dose of 100 mg/kg in comparison with a Rhaponticum carthamoides extract (ERC) on the effectiveness of an aerobic–anaerobic training regimen in female mice in a triple-weight loaded exhaustive swim test with a 10% load of body weight, as well as on the level of malondialdehyde and catalase activity in blood hemolysate, cardiac and skeletal striated muscle tissue, and liver homogenate. The results obtained indicate a reduced severity of lipid peroxidation under the influence of dry EAR and EAT extracts, thus pointing to the antioxidant activity of the extracts. In the conducted triple-weight loaded exhaustive swim test, a statistically significant increase in the duration of swimming No. 3 and the test index was noted in both groups compared to the control. Therefore, it can be concluded that the studied extracts have a positive effect on the first-phase recovery processes.

Influence of Wormwood Seeds on Enzymatic and Non–enzymatic Activity in Blood of Broilers with Coccidiosis

Wormwood plants have been of great botanical and pharmaceutical interest and are employed in folk medicine for a long time. Wormwood has been used successfully for many years as a remedy against malaria and has been reported to exhibit antiprotozoal potential. Having in mind that, this study aimed to investigate the effects of wormwood seeds on enzymatic activity and non–enzymatic activity in broilers infected with coccidiosis. A biological experiment with broiler chickens was performed on a total of 360 Ross 308 broiler chickens. Chickens were divided into three experimental treatments with six replication each, respectively. Treatment I (WW1) was uninfected with coccidia oocyst and untreated, treatment II (WW2) was infected with coccidia oocyst and was kept untreated, while treatment III (WW3) was infected with coccidia oocyst and received wormwood seeds in feed in the quantity of 1.5%. Blood was collected for the evaluation of antioxidant status. Results of bloody diarrhea intensity of chickens have ranged from 0% (WW1; day 26th) to 99% (WW2; day 28th), respectively. Regarding the influence of dietary wormwood seeds on fecal oocyst counts obtained results showed the highest oocyst in treatment WW2 on the 30th day of the experiment (45115.7 oocyst number/g), which was significantly higher (p<0.05) when compared to treatments WW3 (623.3 oocyst number/g), and WW1 (0 oocyst number/g) on the same experimental day. Our results indicate a significant (p<0.05) increase in non-enzymatic activity and higher catalytic enzymatic activity in blood hemolysates of infected broilers. Based on our research it can be concluded that wormwood seeds can be used in feed for broilers as a prophylactic feed additive to prevent coccidia oocyst development.

Effects of triterpenoids in fatty products on liver condition of laboratory animals with acute toxic hepatitis

One of the principles of prevention and non-medicamentous treatment of liver diseases, including hepatitis of different etiology, is the normalization of the diet through the consumption of food with physiologically active ingredients, in particular betulin, which helps to eliminate the causes of metabolic and oxidative disorders within liver cells. The aim of the research was to assess in vivo the influence of triterpene alcohol betulin extracted from Betula pendula Roth. birch bark in fat-containing products (for example mayonnaise) on the blood biochemical parameters and liver morphological structure of rats with initiated acute toxic hepatitis. Material and methods. Hepatoprotective and antioxidant activities of betulin as part of mayonnaise samples has been investigated in vivo on the model of toxic hepatitis initiated by carbon tetrachloride in male Wistar rats weighing 210-265 g. The animals were divided into 4 groups of 10 animals each: CG-1 - intact, CG-2 and MG - with carbon tetrachloride initiated toxic hepatitis. rats of the main groups were orally administered mayonnaise once a day at a dosage of 1 ml for 21 days after the formation of the model pathology: OG-1 with the added betulin (1 mg per 1 kg of body weight), OG-2 without betulin. Disorders of metabolic and oxidative processes in liver cells of animals were evaluated by biochemical indicators of blood plasma: the level of glucose, albumin, total cholesterol, triglycerides and urea and the activity of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ-glutamyltransferase. Oxidative stress in rats was estimated by the activity of catalase and superoxide dismutase in blood hemolysate (at a dilution of 1:200 and 1:10, respectively); the total prooxidant (in blood plasma) and total antioxidant (in blood hemolysate at a dilution of 1:10) activity were determined spectrophotometrically (colored complexes of TWIN-80 oxidation products with thiobarbituric acid). The morphological structure of rats' liver was estimated by microscopy of prepared cuts of hepatic tissue. Results. Based on biochemical parameters of rat blood plasma, it has been established that the administration of mayonnaise with betulin prevents the development of cytolic syndrome and suppresses the process of peroxidation by directly neutralizing free radicals. Aspartate aminotransferase and alkaline phosphatase activity in blood plasma of the experimental animals of the main group MG-1 reduced by 20.7 and 35.2% compared with indicators of the rats of the main group MG-2. Glucose concentration normalized to the level of the control group CG-1. The concentration of bilirubin and triglycerides decreased by 22.9 and by 48.1%, which indicates a significant reduction in the indicators of cholestatic syndrome in the group of animals OG-1 compared to OG-2. The total prooxidant activity and the concentration of thiobarbiturate-reactive products decreased compared to the CG-2 and MG-2 groups, which indicates the suppression of oxidative stress and, as a result, an improvement in liver conditions of animals with toxic hepatitis even when taking a fat-containing product. In liver histopeparates of animals receiving mayonnaise with betulin, necrobotic changes were less pronounced in comparison with the group MG-2. They were estimated at 1 point: small-drip dystrophy spots were found, haemorrhages in the interregional septum with inflammatory infiltration in the course of hemorrhages against the presence of necrosis hepatocytes with pronounced adipose dystrophy in the centres of the lobules, step necrosis with signs of replacing the damaged hepatocytes of the connective tissue, accompanied by centrolobular hemorrhages in MG-2 rats. Conclusion. Introduced into the composition of mayonnaise betulin, reduces the development of cytolic syndrome in toxic hepatitis and suppresses the process of peroxidation, on the basis of which fat-containing foods with betulin can be recommended for clinical examination as specialized products in acute and chronic liver diseases, including complicated cholestasis.

Quantification of human embryonic ζ-globin chains in Southeast Asian deletion (--SEA) carriers

AimsReactivation of embryonic ζ-globin is a promising strategy for genetic treatment of α-thalassaemia. However, quantification of ζ-globin as a quantitative trait in α-thalassaemia carriers and patients remains incompletely understood. In...

Sulfanilamide Modified Magnetic Nanoparticles for Purification of Carbonic Anhydrase from Bovine Blood.

Magnetic nanoparticles (MNPs) have been used for purification of specific biomolecules form mixtures. The aim of this study is to develop a new, cheap, reusable, and magnetic-based material to purify the carbonic anhydrase (CA) enzyme in a short time with high efficiency. In the first part of this study, silica-coated iron oxide magnetic nanoparticles (Fe3O4@SiO2 MNPs) were obtained. Surface modification of Fe3O4@SiO2 MNPs was accomplished with 3-(4-Hydroxyphenyl) propionic acid (PA) and sulfanilamide (SA), respectively. SA is a selective inhibitor of CA, and it selectively binds to CA. The final particle was named Fe3O4@SiO2-PA-SA MNPs and characterized by SEM, TEM, XRD, and FT-IR. It was determined that the produced MNPs contained multicore, were smaller than 100nm in size, and had a spherical morphology. The CA was purified from bovine blood hemolysate in a short time such as 2.5h and in a simple manner. The maximum enzyme purifying capacity of MNPs was calculated as 13.87 ± 3.27mg CA/g MNP. SDS-PAGE analysis was confirmed that high CA purification success was achieved.

Effect of Lipid Peroxidation on Dental Healthcare Workers.

ABSTRACTAim and Objective:The relevance of the study was explained by the fact that free radicals, known to be a product of lipid peroxidation, damage the integrity of cell membranes and corresponding intracellular structures, disrupting their functioning. The purpose of this cross-sectional study was to investigate the effect of free-radical lipid peroxidation in the blood on the body of dentists without diseases of the bronchi and lungs.Materials and Methods:The chemiluminescent properties of the blood hemolysate of 65 dentists were measured. Blood was collected in a test tube with an anticoagulant, and the plasma was aspirated with a Pasteur pipette. The hemolysate was aspirated two times. Distilled water was added to the sediment of erythrocytes, the mixture was shaken, and centrifuged. Statistical processing of morphometric indicators was carried out using the software package “Statistica 6.0.”Results:The direct dependence of the spontaneous chemiluminescence (SCL) growth parameters in the blood hemolysate of dentists on their length of service was determined.Conclusion:The conclusions indicate a direct correlation between the growth parameters of the SCL index in the blood hemolysate of dentists and their length of service. The applied value of this study lies in the possibility of practical application of the results obtained to qualitatively investigate the effect of lipid peroxidation processes on the body of dentists.

The effect of ethanol on protein-ligand interactions

The relevance of studying the regulation of protein-ligand interactions is due to the emergence of new views on the role of metabolites and their key importance in vital processes. To study the protein-ligand interaction, the AB0 antigen-antibody blood system and the enzyme-substrate system of dehydrogenases were used as a test system, and ethanol was used as an influencing factor. In experiments performed with A and B blood erythrocyte antigens, natural AB0 system antibodies and monoclonal antibodies under the influence of ethanol performed change of the degree of agglutination and the time to onset of erythrocyte agglutination. It was found that ethanol can regulate the enzyme-substrate interactions of dehydrogenases: lactate dehydrogenase (EC 1.1.1.27), glyceraldehyde phosphate dehydrogenase (EC 1.1.1.12), and α-glycerol phosphate dehydrogenase (EC 1.1.1.8). The increase in the activity of studied enzymes under the influence of ethanol in the whole blood hemolysate was 2.5 - 3 times higher than in the isolated medium (with pure enzyme preparations).

Comparative analysis of the parameters of carbohydrate exchange and thiamine security in the crew personnel of the northern water basin depending on the specifics of work, age and professional experience

Purpose: assessment of carbohydrate metabolism and thiamine supply in the crew, depending on the specifics of work, age and professional experience.Materials and methods. The researchers examined the crew personnel of the Northern Water Basin (river workers, fishermen and seafarers) aged from 21 to 59 years, with professional experience up to 35 years. In the blood serum, the parameters of carbohydrate metabolism were determined by the spectrophotometric method, and in the blood hemolysate, by the fluorometric method, the content of the thiamine in the body.Results and its discussion. Seafarers have an increased glucose concentration against the background of low thiamine content relative to fishermen and river workers. The frequency of occurrence of marginal states increases with increasing age and experience in river workers, and the frequency of occurrence of pronounced thiamine-deficient states increases with increasing experience in fishermen. Changes in carbohydrate metabolism and thiamine content of the crew depend mainly on the specifics of the work.

Level of biogenic amines for pregnant women with psycho-emotional disorders stipulated by anxiety

The objective: determine level of biogenic amines for pregnant women with psycho-emotional disorders stipulated by anxiety. Materials and methods. 90 Pregnant women were examined in ІІ and ІІІ trimester of pregnancy. The main group includes 58 pregnant women with the medium and high level of state anxiety and 32 pregnant women with anxiety level of 30 points and less that indicates the low level of SA (control group). For estimation of the state anxiety and trait anxiety the scale of Spielberger-Hanin was used. Level of serotonin and melatonin was measured with fluorometric method in blood hemolysate of pregnant women. Results. According to the obtained results of biochemical research of hemolysate of packed red cells of blood of examined women there was stated that increase of state anxiety was accompanied by statistically reliable (p<0,05) rise of serotonin level and decrease of melatonin level. Thus, special features have direct influence on increase (p<0,05) of serotonin/melatonin index. Pregnant women of the main group have the index which was 2.5 time higher than the same index for women of the control group. Conclusions. Results of performed research among pregnant women have indicated that increase of state anxiety is accompanied by statistically reliable (p<0,05) rise of serotonin content in blood and statistically reliable (p<0,05) decrease of melatonin. Such results have direct influence on increase (p<0,05) of serotonin/melatonin index for pregnant women with medium and high levels of state anxiety comparing to the certain index for women with the low level of anxiety.

The effect of two formulations of carbon enterosorbents on oxidative stress indexes and molecular conformation of serum albumin in experimental animals exposed to CCl4

The liver failure means inability to perform its normal synthetic, biotransformation and excretory functions. The disturbance of metabolic processes leads to the development of "metabolic endogenous intoxication" resulting in oxidative stress. Oxidative stress initiates the processes of oxidation of amino acid residues of blood plasma proteins causing the changes in their structure and functions. The effect of administration of highly activated porous carbonic enterosorbents on oxidative stress manifestations and molecular conformation of serum albumin in blood of experimental animals with acute liver failure induced by carbon tetrachloride (CCl4) needs to be investigated. Two forms of activated carbonic enterosorbents such as AC1 (primary beads with the range of diameters of 125–250 μm) and AC2 (secondary granules prepared from micronized AC1 having the mean particle size of ~1 μm) derived from phenol-formaldehyde resin were used in rat model with CCl4 intoxication. The total level of reactive oxygen species (ROS) in blood plasma, the activity of catalase (CAT) in blood hemolysates; the content of reduced glutathione (GSH) in liver homogenates, and the level of oxidative modification of proteins (OMP) such as aldehyde-dinitrophenylhydrazone (A-DNPH) and ketone-dinitrophenylhydrazone (K-DNPH) derivatives in blood plasma and liver homogenates were determined. In addition, the level of pro/antioxidant ratio in blood hemolysates and the content of lipid peroxidation product - malondialdehyde (MDA), in blood plasma and liver were determined. Melting thermograms of blood plasma proteins (BPP) and molecular conformation changes of serum albumin were analyzed by biophysical methods (differential scanning microcalorimetry and spectrofluorimetry). The extent of CCl4-induced oxidative damage in blood and liver of experimental animals was shown to be less expressed for AC1 in comparison with AC2 enterosorbent. However, AC2 used in the form of secondary granules positively influenced some biophysical properties of albumin molecule (temperature of melting, shape of melting endotherm and intrinsic fluorescence) after rats exposure to CCl4. In general, administration of both AC1 and AC2 led to the reduction of oxidative stress manifestations and partial restoration of native molecular conformation of serum albumin. These observations are promising in terms of achieving recovery of detoxification potential of organism after severe liver injury.

Effect of Primary and Secondary Beads of Carbon Enterosorbent on Haematological Parameters and Oxidative Stress Development Caused by Melphalan in Rats.

Background and Objectives: Side effects of anti-cancer drugs are usually accompanied by oxidative stress, including myelotoxicity. We evaluated the potential of oral highly activated micro-/macroporous carbon adsorbents (bulk density of 0.16 g/cm3, surface area calculation by Brunauer–Emmett–Teller model (SBET) > 2200 m2/g, derived from proprietary phenolic resin beads) to alleviate oxidative stress and myelotoxicity in rats. Materials and Methods: A single injection of cytostatic melphalan (L-PAM) at a dose of 4 mg/kg was used for modelling. Two forms of activated carbon were used: AC1—primary beads with the particle size range of 125–250 µm, and AC2—micronized AC1 with a mean particle size of ~1 µm. We measured haematological parameters white blood cells, red blood cells, platelet count, and haemoglobin level. Oxidative stress intensity was evaluated using the following markers: total levels of reactive oxygen species (ROS) in blood plasma; catalase activity (CAT) and pro-oxidant/antioxidant ratio in blood haemolysate samples; level of reduced glutathione (GSH) in liver tissues; oxidative modification of proteins, OPM (APHD, aldehyde–dinitrophenylhydrazone derivatives and KPHD, ketone dinitrophenylhydrazone derivatives) and malonic dialdehyde (MDA) in blood plasma and liver samples. Results: AC2 administration promoted significant myeloprotective effect: 1.5-fold increase in leukocytes, 2-fold in neutrophils, 1.5-fold in lymphocytes, and 1.23-fold in platelet count compared to the experimental Melphalan Group. At the same time, AC1 administration resulted in a slight increase in haematological parameters. Both ACs positively corrected important, but diverse, components of oxidative stress. They significantly reduced oxidative modification of blood and liver proteins (especially the AC1 form), normalized the level of reduced glutathione, pro-oxidant/antioxidant ratio and other markers. For some markers, such as ROS production in blood plasma, the use of enterosorbents resulted in non-significant a shift towards normal parameters. Conclusions: Oral activated carbon adsorbents reduce oxidative stress intensity and myelotoxicity; they can be promising means to combat the adverse effects of chemotherapy in clinical practice.

Using the hemoglobin-binding Staphylococcus aureus protein IsdH to enable plasma analysis of hemolyzed blood samples.

Background Intravascular hemolysis and in vitro hemolysis are prevalent contributors to failed blood sample analysis in the routine hospital laboratory. Interferences by hemoglobin in spectrophotometric and certain enzyme activity assays is the major causative factor. Methods By exploiting the hemoglobin-binding properties of the iron-regulated surface determinant H (IsdH) protein from Staphylococcus aureus we have developed a new method to instantly remove hemoglobin and hemoglobin-haptoglobin complexes from plasma in vitro thereby enabling the measurement of hemoglobin-sensitive analytes in hemolyzed plasma. In the present study we used an engineered IsdH mutant form conjugated to Sepharose for the efficient removal of plasma hemoglobin in concentrations up to 15 mg/mL. The high abundance of haptoglobin, which forms a tight complex with hemoglobin in plasma, did not affect the hemoglobin removal by IsdH Sepharose. Results Applying the method on plasma samples that beforehand were spiked with blood hemolysate re-enabled measurement of the hemolysis sensitive parameters: alkaline phosphatase, conjugated bilirubin, iron, ferritin, γ-glutamyltransferase, total thyroxine and troponin T. IsdH Sepharose-mediated hemoglobin removal also enabled measurement of hemolysis sensitive parameters in hemolyzed samples from anonymized patients. Conclusions In conclusion, IsdH Sepharose is a simple cost-effective pretreatment of hemolyzed samples correcting and enabling the measurement of several important hemoglobin-sensitive parameters in a way compatible with standard procedures in routine laboratories.

Antioxidant Enzyme Activities in Rabbits Under Oxidative Stress Induced By High Fat Diet.

IntroductionThe aim of this study was to investigate whether the type and form of oil (raw/non-oxidised (N) or post-frying/oxidised (O)) consumed in high-fat diets affect the oxidative status of an organism, as observed by malondialdehyde (MDA) concentration as an oxidative factor and antioxidant enzyme activity.Material and MethodsFats in the diet came from rapeseed oil (R) and olive oil (O).ResultsThe applied diet caused a decrease in MDA concentration (μmol/L) in serum in group RN from 2.94 ± 0.87 to 1.76 ± 0.13, in group ON from 2.45 ± 0.62 to 1.50 ± 0.10, and in group OO from 2.70 ± 1.16 to 1.84 ± 0.36. Meanwhile, MDA concentration (mmol/L) increased in blood haemolysate in group RO from 0.15 ± 0.07 to 0.22 ± 0.03 and in group OO from 0.17 ± 0.02 to 0.22 ± 0.02. The observed changes caused a response of the enzymatic antioxidant system in both models, especially followed by an increase in activities of total superoxide dismutase and its mitochondrial isoenzyme in all experimental groups, while its cytosolic isoenzyme activity increased only in ON and OO groups. Increased activity of glutathione peroxidase (GPX) in groups RN and RO and of catalase (CAT) in groups ON and OO was observed. Significant differences in responses to the different types and forms of oils were probably caused by the different oxidative stability of the studied oils.ConclusionThis diet disturbed the body’s oxidative status; however, during the six-month study the enzymatic antioxidant system remained effective.

Dynamics of prooxidant and antioxidant homeostasis rates in patients with rosacea in the process of integrated treatment

Objective — to determine the dynamics of prooxidant and antioxidant homeostasis rates in patients with rosacea by using an antioxidant drug in their multimodality treatment.Materials and methods. The study involved 64 patients with rosacea (49 women and 15 men), aged 28 to 63; 23 of them were diagnosed with erythematous­telangiectastic and 41 with papulo­pustular stage (form) of rosacea. In the course of treatment the patients with rosacea were divided into 2 groups: group І (comparative reference group) consisted of 32 patients who received a standard treatment of dermatosis, group ІІ (main group) included 32 patients, who were administered an antioxidant ethylmethylhydroxypyridine succinate (mexidol) in addition to the standard therapy. The status of the blood prooxidant system was assessed by the content of malonic aldehyde (MA) in plasma and in red blood cells and the fractions of protein oxidative modification of the neutral (POM Е370) and the main (POM Е430) nature — in the blood serum; while the status of endogenous intoxication was evaluated by the content of the midmolecular peptides — medium mass molecules (MMM) in the blood serum; the status of the antioxidant blood system — by the reduced glutathione (RG) rate in the blood hemolysate and by the content of ceruloplasmin in the blood serum according to available methods. Results and discussion. Before the treatment the patients with rosacea in both comparative and main groups had a reliable (р < 0.05) compared to the values in the individuals of the control group, increase in the content of MA in plasma (by 53.9 and 51.9 % respectively) and in the red blood cells (by 24.3 and 23.5 %), in the blood serum — POM Е370 (76.7 and 70.9 %), POM Е430 (77.9 and 83.0 %), in the serum — МMМ Е254 (by 46.5 and 42.1 %) and МMМ Е280 (68.6 and 72.2 %), as well as an increase in CА (78.2 and 72.7 %) and a decrease in the content of RG in the blood red cells (by 21.2 and 20.6 %). The patients of the I group had a reliable decrease in the blood serum POM Е370 (9.4 %, р = 0.012) and CА (15.3 %, р = 0.005) only, while the patients of the main group, who were additionally administered the antioxidant drug, had a reliable (р < 0.05) decrease in MA in their plasma (by 16.2 %) and in the red blood cells (13.1 %), in the blood serum — POM Е370 (25.7 %), POM Е430 (24.9 %), МMМ Е254 (23.5 %), МMM Е280 (18.7 %) and CА (25.1 %, р = 0.005), as well as an increase in the content of RG in the red blood cells (10.7 %, р = 0.011). The patients of the main group also had better clinical results after completing the complex treatment.Conclusion. Administrating the antioxidant ethylmethylhydroxypyridine succinate (mexidol) to the patients with rosacea additionally to a standard therapy contributes to the normalization or trends towards to the normalization of prooxidant and antioxidant homeostasis rates and improves clinical outcomes of treatment of such patients.

Level of biogenic amines in pregnant women with psycho-emotional disorders stipulated by anxiety

Object of research – to determine level of biogenic amines in pregnant women with psycho-emotional disorders stipulated by anxiety. Group of examined women and methods of research . 90 Pregnant women were examined in ІІ and ІІІ trimester of pregnancy. The main group includes 58 pregnant women with the medium and high level of state anxiety and 32 pregnant women with anxiety level of 30 points and less that indicates the low level of SA (control group). For estimation of the state anxiety and trait anxiety the scale of Spielberger-Hanin was used. Level of serotonin and melatonin was measured with fluorometric method in blood hemolysate of pregnant women. Results . According to the obtained results of biochemical research of hemolysate of packed red cells of blood of examined women it was stated that increase of state anxiety was accompanied by statistically reliable (p < 0.05) rise of serotonin level and decrease of melatonin level. Thus, special features have direct influence on increase (p < 0.05) of serotonin/melatonin index. Pregnant women of the main group have the index which was 2.5 times higher than the same index in women of the control group. Conclusions . Results of performed research among pregnant women have indicated that increase of state anxiety is accompanied by statistically reliable (p < 0.05) rise of serotonin content in blood and statistically reliable (p < 0.05) decrease of melatonin. Such results have direct influence on increase (p < 0.05) of serotonin/melatonin index in pregnant women with medium and high levels of state anxiety comparing to the certain index in women with the low level of anxiety.

Comparative analysis of prooxidant and antioxidant homeostasis rates in patients suffering from rosacea with different clinical course of dermatosis

Objective is to determine and analyse prooxidant and antioxidant homeostasis rates in patients suffering from rosacea with different clinical course of dermatosis. Material and methods. The study involved 67 patients with rosacea (51 women and 16 men) aged 27 to 64 years of whom 24 individuals were diagnosed with erythematous-telangiectastic and 43 patients with papulo-pustular stage (form) of rosacea. In 21 patients dermatosis lasted up to 6 months, in 23 individuals – from 6 to 12 months and in 22 of them – for more than a year. The condition of the prooxidant blood system was evaluated by the content of malonic aldehyde (MA) in plasma and in red blood cells and of medium mass molecules (МMМ Е254 and МMМ Е280) – in the serum, while that of the antioxidant system- by the reduced glutathione (RG) rate and by the catalase activity (CA) in blood hemolysate and of ceruloplasmin (CP) in the serum according to the known methods. Results. The patients with rosacea had a reliable increase in MA content in the plasm and in red cells (by 53,3 % and 23,9 % respectively, p 0,05) as well as a decrease in the RG content in red cells (by 20,9 %, p<0,001). The patients with papulo-pustular stage (form) of dermatosis experienced more significant changes in these rates compared to those with erythematous-telangiectastic stage (form) of rosacea as well as in case when dermatosis lasts for more than half a year. Conclusion. The patients with rosacea experienced reliable changes in the rates of prooxidant and antioxidant homeostasis, which is indicative of an increase in the intensity of processes of free radical oxidation of lipid structures and development of the state of endogenous intoxication against the background of an intensity and a decrease in the activity of antioxidant protection factors, which are more significant in patients with papulo-pustular stage (form) of dermatosis and a duration of the disease for more than half a year, which substantiates the differentiated administration of ntioxidants in the comprehensive therapy of such patients.

АНАЛИЗ ИЗМЕНЕНИЙ КОМПОНЕНТОВ СИСТЕМЫ ПЕРЕКИСНОГО ОКИСЛЕНИЯ ЛИПИДОВ И АНТИОКСИДАНТНОЙ ЗАЩИТЫ У ВОЕННОСЛУЖАЩИХ

The article presents the analysis of inspection results of the military personnel who passed a medical examination at the initial military registration, with the call to military service and recognized as health fit for military service. The aim of the research was to study the dynamics of changes in the components of lipid peroxidation and antioxidant defense system of the soldiers during the primary adaptation to military service. Materials and methods: 34 young men (recruits), called up for military service, and 18 military personnel who have served 6 months. As material for biochemical research we used blood plasma and hemolysate. Modern spectrophotometric, fluorometric and statistical methods have been used. Results. In recruits, we noted increased activity of lipids oxidation, low level of blood antioxidant activity and reduced glutathione. In 6 months of the military service we found decreased contents of TBA-active products and improving of the overall antioxidant activity of the blood. Conclusions. Analysis of the data shows significant changes in the indices of lipid peroxidation and antioxidant defense system. The ratio of the activity of oxidation and antioxidant components not only reflects, but also largely determines the rate of metabolism and adaptive capabilities of the organism. The study found that young people, who were recognized as fit for military service due to health criteria for military medical commissions by military commissioners and were called up for military service, have low adaptive capacity and reach the stage of “satisfactory adaptation” only after 6 months of service.

Features of Lipoperoxidation, Antioxidant Defense, and Thiol/Disulfide System in the Pathogenesis of Infertility in Males, Carriers of Nonfunctional Variants of GSTT1 and GSTM1 Gene Polymorphisms.

Comparative analysis of the parameters of LPO, antioxidant defense (AOD), and the thiol/disulfide system was performed in fertile and infertile males of reproductive age carrying different genotypes of the glutathione system genes. Blood plasma, blood hemolysate, and ejaculate served as specimens for biochemical studies. A decrease in glutathione S-transferase activity was found in blood and ejaculate specimens from fertile and infertile carriers of nonfunctional GSTT1(0/0)/GSTM1(0/0) genotypes. In infertile carriers of nonfunctional GSTT1(0/0)/GSTM1(0/0) genotypes determining reduced glutathione S-transferase activity, a decrease in the concentration of low-molecular-weight cell antioxidant (reduced glutathione) and an increase in the concentration of secondary LPO products (TBA-reactive substances) were revealed. Identification of carriers the polymorphic GSTT1 and GSTM1 variants and analysis of activity of the thiol/disulfide system enzymes can be recommended for additional evaluation of the risk for reproductive dysfunction in men.

ПРОЦЕССЫ ПЕРЕКИСНОГО ОКИСЛЕНИЯ ЛИПИДОВ И АНТИОКСИДАНТНОЙ ЗАЩИТЫ У ПРАКТИЧЕСКИ ЗДОРОВЫХ МУЖЧИН РАЗЛИЧНЫХ ЭТНИЧЕСКИХ ГРУПП

The research is devoted to studying the features of antioxidant protection system in fertile men of various ethnic groups. Studying the features of antioxidant protection processes in the populations of various ethnic origins, living in identical geographical conditions, is of scientific interest. 126 men of reproductive age (84 men of Russian population and 42 men of Buryat population living in Ulan-Ude) were examined. Blood plasma and hemolysate were used as the material for the biochemical research. Modern spectrophotometric, fluorometric and statistical methods were used. The basic indicators of lipid peroxidation, reflecting different stages of oxidation (рrimary,, intermediate, final products] and total antioxidant activity of blood, which characterizes the total activity of inhibitors of radical oxidation, and determining its buffering capacity were studied. We investigated the basic units of antioxidant protection - enzymatic (superoxide dismutase) and non-enzymatic (low molecular weight soluble vitamins retinol and а-tocopherol, oxidized and reduced glutathione). The peculiarities of Ирid peroxidation and antioxidant protection processes in fertile men have significant differences. They can be attributed to the ethnic origin and characterize various degrees of activity of metabolic processes in the surveyed groups. Studying the condition of antioxidant protection system will significantly expand ideas of organism adaptation opportunities to external conditions and will be able to become a basis for effective monitoring of reproductive health.

ОСОБЕННОСТИ АНТИОКСИДАНТНОГО СТАТУСА ПОДРОСТКОВ С АРТЕРИАЛЬНОЙ ГИПЕРТЕНЗИЕЙ

One of the most topical problems in the field of healthcare is to solve the issues of an early diagnosis, timely prevention and effective treatment of cardiovascular diseases, which include arterial hypertension (the AH]. Prevalence of EAH in the pediatric population in Russia amounts to 20 %. Despite the fact that the mechanisms of the disease were studied in detail, metabolic changes that led to its early deve^ment in teenagers were still not entirely clear. In the Clinic of FSBSI Scientific Center for Family Health Problems and Human Reproduction (Irkutsk], clinical and functional examination of 48 male adolescents aged 14-17 years, of which 25 patients with stable arterial hypertension and 23 adolescents without somatic pathology with normal blood pressure, was conducted. Antioxidant status was studied in blood plasma and hemolysate. A comparative analysis of the following indicators was conducted: total antioxidant activity, superoxide dismutase activity, level of retinol and α-tocopherol, glutathione system parameters. Patients with stable arterial hypertension were recorded with level of overall antioxidant activity in blood 29 % higher than the benchmark, decreased superoxide dismutase activity by 11 % and content of а-tocopherol by 44 %. Retinol concentration in teenagers with hypertension was two times higher than the content of this indicator in the control group (р = 0.00]. Decrease in the antioxidant levels was found in adolescents with arterial hypertension that can be the cause of the oxidative stress development.