Blood Hematology Research Articles

The Immune, Inflammatory and Hematological Response in COVID-19 Patients, According to the Severity of the Disease.

The aim of this study was to evaluate the immune and inflammatory responses in COVID-19 patients by dosing specific IgM and IgG total antibodies and interleukin 6, correlating them with the hematological and biochemical blood parameters and comparing them by the form of the disease. One hundred twenty-five patients with polymerase chain reaction-confirmed COVID-19, hospitalized between 15.03.2020 and 1.07.2020 in the Clinical Hospital of Infectious Diseases "Sf. Parascheva" Iaşi, were tested by chemiluminescence for the presence of anti-SARS-CoV-2 IgM and IgG and IL-6 in the serum. The results were correlated with the results of the CBC count and serum biochemical parameters detected on the admission day. The patients presented different forms of the disease (asymptomatic, mild, moderate, severe, and critical) according to World Health Organization (WHO) criteria for the clinical management of COVID-19. The amplitude of the immune response was directly correlated with the form of the disease. In the asymptomatic/mild form patients, the IL-6 and CRP concentrations were significantly higher and eosinophil count was significantly lower compared with the reference interval. In the moderate form, the concentrations of IL-6, CRP, and IgG were significantly higher, compared with the reference interval, while eosinophil count and eGFR were significantly lower. In severe/critical COVID-19 patients, IL-6, CRP, NLR, PLR, glucose, AST, urea, creatinine, and eGFR were significantly higher compared with the reference interval, while eosinophil count was significantly lower. IL-6 boosted in all forms of COVID-19, with a major increase in severe and critical patients. IL-6, neutrophil count, % neutrophils, NLR, PLR, CRP, AST, and urea increased with the severity of the SARS-CoV-2 infection, and the lymphocyte count, % lymphocytes, eosinophil count, % eosinophils, and hemoglobin decreased with the increased severity of COVID-19. The amplitude and the moment of appearance of the immune response depended on the form of the disease. IgM generally occurred in the first 14 days of illness, and IgG appeared beginning with the second week of disease. IgG titer increased rapidly until the fourth week of disease and decreased slowly after 4 weeks. The amplitudes of all the tested inflammatory and serological markers depended on the COVID-19 form, increasing somewhat in the moderate forms and even more in the critical ones. The lymphocyte and eosinophil count are able to predict the risk of severe COVID-19.

Influence of Organic Iron Enriched Diets on Performance, Egg Quality, Blood Haematological and Biochemical Constituents in Quails

The current research was carried out to investigate the effect of diets enriched with different levels of organic iron on performance, egg quality, blood haematological parameters, and serum biochemical constituents in laying quails. In the study, 120 female quails at 20 weeks of age were allocated to 5 treatment groups with 6 replicates of 4 quails in each. Experimental diets were formed by adding 0, 50, 100, 150, and 200 mg/kg of iron (iron-glycine) to the diet containing 96 mg/kg of iron. Performance parameters of quails were not affected by the supplementation of organic iron to diet (P>0.05). The supplementation of organic iron affected as quadratic only the eggshell breaking strength among the egg external quality parameters, and it was maximum at 150 mg/kg organic iron addition (P<0.01). Egg yolk a* value, which is one of the egg internal quality parameters, was linearly affected by organic iron addition, but other parameters were not affected (P<0.01). The administration of organic iron to the diet linearly increased the serum iron level (P<0.01), the serum phosphorus level was affected quadratically (P<0.05) and reached a maximum at 150 mg/kg. Among the blood haematology parameters, white blood cell and monocyte counts linearly increased with the addition of organic iron to the diet (P<0.01), but other parameters were not affected. According to the results obtained from current research, it can be said that enrichment of laying quail diets with organic iron up to 150 mg/kg level improved eggshell breaking strength without affecting performance, however, it negatively affected blood parameters by increasing white blood cell and monocyte without changing blood red cell parameters.

Determination of Platelet Count Estimation Factor on Peripheral Blood Smear Confirmation Using Field Number 22 Microscope

The automatic platelet count sometimes requires confirmation on the peripheral blood smear. Platelet count estimation can also be used for reporting platelet count if an automatic cell counter is not available, with an estimation factor according to the Field Number (FN) of the microscope used. This study aimed to determine the platelet count estimation factor based on peripheral blood smear confirmation using an FN 22 microscope. An observational cross-sectional study was carried out in patients who had routine hematological and peripheral blood smear examinations during September 2021 by determination of platelet count using the automatic cell counter method and an average number of platelet counts per field of view with 100x objective magnification. The estimation factor is the total ratio divided by sample size. The total ratio of 254 samples was 4.086. The platelet count estimation factor was 16, indicating that 1 platelet per field of view was equivalent to 16x103/µL. There was a very strong significant correlation between mean platelet count per field of view and platelet count using the automatic cell counter (p<0.001, R>0.750). The field number is the image diameter of the microscope eyepiece. The latest generations of microscope use FN 20 or more, which provides a wider field of view, enabling the observation of more platelets. Factor estimation was used to determine the estimated platelet count on a peripheral blood smear. A big difference between automatic cell counter and peripheral blood smear might indicate pre-analytic, analytic, and post-analytic errors. The platelet count estimation factor based on peripheral blood smear confirmation using the FN 22 microscope was 16. Each laboratory needs to determine the estimation factor according to the FN microscope used.

Safety assessment of Lepisanthes fruticosa extract in experimental rats

The toxicity study of Lepisanthes fruticosa (ceri Terengganu) was carried out using a repeated dose 28-days oral toxicity method on female Sprague Dawley (SD) rats. About twenty-four female SD rats with an average body weight of 116.27±11.95 g were divided into four groups. The control group (C) was given a ground normal pellet while treatment groups were given L. fruticosa extract incorporated with ground pellet with different dosages (low dose 0.5 g/kg (CEL), medium dose 1.0 g/kg (CEM) and high dose 3.0 g/kg (CEH)). Each rat was given a 25 g pellet per day. Upon arrival, all rats were placed in individual cages and given drinking water via ad-libitium. Rats were acclimatized for seven days before treatment started. On day 29, after 12-15 hrs of fasting, rats were euthanized using CO2 and blood samples were taken from the vena cava for further blood and serum analysis. After 28 days of treatment, the body weight of rats in all treated groups was increased with no significant difference. Administration of L. fruticosa extract did not cause any negative effect on blood haematology. Findings from the serum biochemical test showed that the consumption of the extract did not result in liver and kidney failure since no significant changes were observed in enzymatic and blood parameters. Based on these findings, the L. fruticosa extract was safe to be taken even at a high dose (3.0 g/kg) as no experiment-related mortality and no toxicity symptom occurred on the experimental animal.

Influence of the Level of Sex Hormones in the Blood of Gilts on Slaughter Characteristics and Meat Quality.

The aim of the conducted research was to determine the impact of factors such as sex hormone levels, which vary during gilts' reproductive cycle, on the quality of the obtained meat and slaughter characteristics of the processed gilts. The research material included a population of 60 gilts slaughtered in one of the slaughterhouses located in south-eastern Poland. After the slaughtering operations were completed, the carcasses were weighed at the classification stand. The results of the statistical evaluation of the haematological and biochemical blood parameters of the examined gilts showed that, in the tested blood samples, the concentration of progesterone had a statistically significant impact only on the level of total protein, which was higher in the blood samples of gilts with a low concentration of progesterone. It was found that carcasses of gilts with higher levels of the LH hormone were characterized by a lower meat content index by nearly 3%. It was shown that the concentration of LH affected the post-slaughter temperature of the sirloin and ham muscles. The interpretation of the obtained data was difficult since there seems to be a gap in the literature concerning the dependencies of sex hormone levels in gilts and meat quality.

The study of the effect of drinks based on extracts of herbal adaptogens on the functional status of athletes during physical activity

Adverse environmental factors, stress, lack of sleep and rest, and heavy physical exertion, deplete the human body. In particular, the reserves of the main metabolites, water, and oxygen, are very limited. People, especially athletes, need to take special dietary supplements with adaptogenic properties to adapt to stressful extreme loads. In this study, the influence of using extracts of leuzea, ginseng, and Eleutherococcus on athletes' performance, endurance, strength, and emotional state is carried out. The studies were conducted on four groups of male athletes aged from 19 to 25 years. For three weeks, diagnostics of vital lung capacity, Stange, and Genchi tests are carried out, and data on the general impressions of athletes are collected. According to the research results, the use of adaptogens leads to an increase in physical performance. After the first week of the study, a positive effect on the human body are noticed: improve well-being and increased athletic performance. When using Eleutherococcus, there was a change in the work of the central nervous system (motor functions): tasks begin to be performed in an organized and accelerated manner without deterioration of well-being, but the volume of strength exercises remained the same. When using the drug leuzea, muscle strength was noted, which allowed to increase the load. There is a positive effect of phytopreparations on the body, namely on the functions of the cardiovascular, central nervous and endocrine systems. In 4 participants who took leuzea, the performance in power competitions improved by 18.5% compared to the control group. The intake of Eleutherococcus and ginseng is accompanied by an increase in the activity of neurotransmitter cells, i.e., the effect on the mesolimbic system. In addition, a study of hematological blood parameters and hormonal statuses at the beginning and end of the study was conducted with the subjects who took leuzea extract. So, the use of the drug leuzea leads to the following positive changes: a more significant increase in ESR, a more significant increase in hemoglobin, compared with the control group. The conclusion is made about the practicality of taking biologically active additives based on some plant adaptogens.

Maternal nutrient restriction during late gestation reduces vigor and alters blood chemistry and hematology in neonatal beef calves.

Fall-calving primiparous beef females [body weight: 451 ± 28 (SD) kg; body condition score: 5.4 ± 0.7] were individually-fed either 100% (control; CON; n = 13) or 70% (nutrient restricted; NR; n = 13) of metabolizable energy and metabolizable protein requirements for maintenance, pregnancy, and growth from day 160 of gestation to parturition. Calves were reared naturally by their dams and monitored for latency times from birth to first sternal recumbency, attempt to stand, and stand; vigor scores were assigned at 2, 5, 10, and 20min of age. Rectal temperatures and jugular blood were obtained at 0 (pre-suckling), 6, 12, 24, and 48h of age, and blood chemistry, hematology, cortisol, and insulin were determined. Data were analyzed with fixed effects of late gestational nutritional plane (single data point) or nutritional plane, hour, and their interaction (data over time, repeated measures). Calving date was a fixed effect; calf sex was included when P < 0.25. We previously reported that late gestational nutritional plane did not affect gestation length or calf size at birth, but calving assistance and fetal malpresentations occurred more often in NR. Nutritional plane did not affect (P = 0.65) duration of parturition, but calves born to NR dams had slower times to attempt to stand (P = 0.09), slower times to stand (P = 0.02), and had poorer 20min vigor scores (P = 0.05). Serum immunoglobulin G and A concentrations at 48h were greater (P ≤ 0.03) for NR calves. Rectal temperature of NR calves was less (P = 0.02) at 0h, but greater (P = 0.04) at 24h compared with CON. Circulating glucose, non-esterified fatty acids, triglycerides, cortisol, and insulin were not affected by nutritional plane (P ≥ 0.18). Total protein and globulin from 6 to 48h were greater (P ≤ 0.02) in NR calves. Calves from NR dams had greater (P < 0.08) gamma-glutamyl transferase at 6, 12, and 48h. Serum aspartate aminotransferase was greater (P ≤ 0.07) from 0 to 24h and creatine kinase was greater (P ≤ 0.04) from 6 to 24h in NR calves. At 0h, potassium was greater (P = 0.03) in NR calves. Calves born to CON had greater chloride (P = 0.08; main effect), sodium (P ≤ 0.09) from 0 to 48h, and anion gap (P = 0.02) at 6h. Hematocrit from 6 to 24h and red blood cells and hemoglobin at 6 and 12h were greater (P ≤ 0.09) in CON calves. These data indicate that nutrient restriction during late gestation resulted in less vigorous calves with more indicators of trauma in early life.

Effects of &lt;i&gt;Trichinella spiralis&lt;/i&gt; and &lt;i&gt;Echinococcus multilocularis&lt;/i&gt; extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice

The purpose of the research is to study effects of Trichinella spiralis and Echinococcus multilocularis extracts on mitosis in a bone marrow cell population, and on hematological and biochemical blood parameters of mice after single and multiple intraperitoneal injections.Materials and methods. The experiment was conducted on outbred male mice. The T. spiralis and E. multilocularis extracts were administered intraperitoneally once or multiple times daily for 10 days at a dose of 80 μg/mouse. Bone marrow cell isolation, microscopic preparations, mitotic index and individual stage determination were made as described in the literature (Ford C. E., Hamerton J. L., 1956). The mouse main peripheral blood parameters were determined with a MicroCC-20 Plus hematological analyzer, and the leucogram was determined by a conventional method. Biochemical blood parameters of the mice were determined with a Clima MC-15 analyzer.Results and discussion. The T. spiralis and E. multilocularis extracts after a single intraperitoneal injection at a dose of 80 μg/ mouse had a pronounced negative effect on the mouse bone marrow cell mitosis with the cell division terminated in the metaphase and a decreased proportion of other mitosis stages. Characteristics were detected of the effects made by the T. spiralis and E. multilocularis extracts on the mouse bone marrow cell mitosis after multiple administration for 10 days. In the reinjection mode of the test extracts to the mice, hematological and biochemical parameters did not change.

Design and evaluation of 3D-printed Sr-HT-Gahnite bioceramic for FDA regulatory submission: A Good Laboratory Practice sheep study.

There is an unmet clinical need for a spinal fusion implant material that recapitulates the biological and mechanical performance of natural bone. We have developed a bioceramic, Sr-HT-Gahnite, which has been identified as a potential fusion device material. This material has the capacity to transform the future of the global interbody devices market, with follow on social, economic, and environmental benefits, rooted in its remarkable combination of mechanical properties and bioactivity. In this study, and in line with FDA requirements, the in vivo preclinical systemic biological safety of a Sr-HT-Gahnite interbody fusion device is assessed over 26 weeks in sheep under good laboratory practice (GLP). Following the in-life phase, animals are assessed for systemic biological effects via blood haematology and clinical biochemistry, strontium dosage analysis in the blood and wool, and histopathology examination of the distant organs including adrenals, brain, heart, kidneys, liver, lungs and bronchi, skeletal muscle, spinal nerves close to the implanted sites, ovaries, and draining lymph nodes. Our results show that no major changes in blood haematology or biochemistry parameters are observed, no systemic distribution of strontium to the blood and wool, and no macroscopic or histopathological abnormalities in the distant organs when Sr-HT-Gahnite was implanted, compared to baseline and control values. Together, these results indicate the systemic safety of the Sr-HT-Gahnite interbody fusion device. The results of this study extend to the systemic safety of other Sr-HT-Gahnite implanted medical devices in contact with bone or tissue, of similar size and manufactured using the described processes. STATEMENT OF SIGNIFICANCE: This paper is considered original and innovative as it is the first that thoroughly reports the systemic biological safety of previously undescribed bioceramic material, Sr-HT-Gahnite. The study has been performed under good laboratory practice, in line with FDA requirements for assessment of a new interbody fusion device, making the results broadly applicable to the translation of sheep models to the human cervical spine; and also the translation of Sr-HT-Gahnite as a biomaterial for use in additional applications. We expect this study to be of broad interest to the readership of Acta Biomaterilia. Its findings are directly applicable to researchers and clinicians working in bone repair and the development of synthetic biomaterials.

Влияние инфузионной терапии на функцию почек и лабораторные показатели в однодневном эксперименте

Changing views on the composition of infusion therapy in medical practice over the last decade is associated with certain side effects of infusion solutions. It is of relevance to study their influence on the kidneys, metabolism, electrolyte composition and, ultimately, on the patient's treatment results. The aim of the present work is to determine the effect of colloid and crystalloid solutions on renal function and laboratory parameters in experiment. The experimental study was performed on 28 rats (males) of Wistar line. Four experimental groups were formed: I control (n=7) — intact animals (without infusions and laparotomy), II (n=7) — laparotomy without infusions, III (n=7) — laparotomy + infusion of 6 ml of 6% hydroxyethyl starch, IV (n=7) — laparotomy + infusion of 6 ml of 0.9% sodium chloride solution. All animals underwent hematologic and biochemical blood tests, morphologic studies of kidneys. The most pronounced changes in blood tests and morphology were revealed during infusion of 6 ml of 0.9% sodium chloride solution. We observed leukopenia, decreased levels of albumin, cystatin C, cholesterol, alpha-amylase activity, increased concentration of bile acids compared to groups I and II. Morphologic study revealed significant dilatation of capsules of a part of tubules with dilated lumen of their capillaries, irregularly expressed full blood vessels. In addition, displacement of nuclei of nephrothelium to the apical pole of the cell was noted in the absence of homogeneity of cytoplasm with the presence of "enlightenments" in it. Infusion of hydroxyethyl starch did not change the main hematologic and biochemical blood parameters, except for the increase in alpha-amylase activity. Morphologic study did not reveal changes that would lead to acute kidney damage. All changes were reversible. Infusion of physiological solution caused shifts in both hematologic and biochemical blood parameters, in particular, hypoalbuminemia. The results of histologic study indicated redistribution of fluid in the interstitium.

Does the Paleo diet affect an athlete's health and sport performance?

The aim of the study was to assess the impact of an eight-week Paleo diet on the health status (body composition, haematology and biochemistry of blood and urine) and the level of physical capacity (aerobic and anaerobic) of professional handball players. Fifteen athletes were assigned to two groups: 9 in the experimental group (PD) and 6 in the control group (CD). Significant decreases in body mass (BM), body mass index (BMI), and fat mass (FM) as well as an increase in the fat-free mass (FFM) (%) in both groups were observed. There were no significant differences between groups in particular series during the experiment in all haematological and biochemical indicators of blood and urine. Only HDL-C was significantly higher in the last series in the PD compared to the CD (1.63 mmol/l vs. 1.23 mmol/l). In the Wingate test, there were only single intragroup changes, consisting of a significant decrease in the Wt, MAP and Pmean in the experimental group. There were no significant differences between the groups in individual series or intragroup differences during the experiment, determined by the VO2max, VEmax, VE ∙ VCO2 -1, RER, and the time of the test with a gradually increasing load on a treadmill, except for a significant decrease of maximum tidal volume (TVmax) in the PD. No adverse effect of the Paleo diet on the health status was found. The use of the Paleo diet slightly adversely affects anaerobic capacity and does not affect the level of aerobic capacity.

Comparison of Cord Blood pH, Haematological Parameters and APGAR Score of New-Born with Maternal and Peri-Natal Risk Factors: A Prospective Observational Study

Introduction: Appearance, Pulse, Grimace, Activity, Respiration (APGAR) score is used worldwide to assess newborn health immediately after birth. The combination of pH analysis, haematological parameters of cord blood samples and APGAR score compared with the maternal and peri-natal risk factors will help us identify the cause of low APGAR. Aim: To compare the pH of cord blood, haematological parameters, Neutrophil-Lymphocyte Ratio (NLR) and APGAR score with high-risk maternal and peri-natal factors. Materials and Methods: This is a prospective observational study, conducted over a period of six months, at Chettinad Hospital and Research Institute, Chennai. The cord blood samples of 40 newborns (20 normal and 20 high-risk pregnancy) were collected for assessing pH and haematological parameters (complete haemogram, nucleated Red Blood Cells (RBCs), reticulocyte count). APGAR scores were obtained from clinical records. The data obtained in the study was analysed using the International Business Management (IBM) Statistical Package for Social Sciences (SPSS) statistics version 23.0. Results: The authors compared 20 cases of newborns with maternal risk-factors with 20 newborns with no risk factors, and found that low APGAR and acidic pH of cord blood, high NLR was associated with risk factors present in the ante-natal and peri-natal period. Conclusion: The present study observed that a low APGAR, low acidic pH of cord blood, high NLR was seen in mothers with adverse risk factors during pregnancy, compared to the mothers with no associated co-morbidities. Hence, the presence of riskfactors during pregnancy should warrant a close follow-up of the new-born in the immediate postpartum period.

The first report of the clinical diagnosis and surgical management of pyometra in two cheetahs (Acinonyx jubatus)

Two female adult cheetahs, residents of the same sanctuary, presented several weeks subsequent to being mated with the same male. Their presenting histories included lethargy and inappetence in both; one female had vaginal discharge, and the other had apparent abdominal pain. Clinical signs supported by laboratory data established a diagnosis of pyometra in both and concurrent septic peritonitis in one case. Diagnosis was facilitated by transabdominal ultrasonography showing a fluid-distended and thickened uterus in both and intraperitoneal fluid accumulation, characterised by abdominocentesis as an exudate, in one case. Peripheral blood smears and haematology showed septicaemia that supported rapid surgical intervention by ovariohysterectomy in both cases. The reported retrospective endocrinological, cytological and bacteriological testing and macro- and histo-pathological investigations and findings confirmed the diagnosis. Pyometra has been widely described in many other wild and domestic carnivores, but there are no previous reports of either the clinical manifestation or management of uterine infections in cheetahs. The subsequent successful induction and anaesthetic protocol and the chosen surgical method for ovariohysterectomy are detailed. Post-operative recovery was rapid in both cases and both were discharged and reportedly healthy at a two-year follow-up. The report discusses the pertinent findings in these cases in relation to the literature describing pyometra, its diagnosis and management in both domestic and other wild carnivores. The report is intended to inform those in the field of management of captive cheetahs to improve their health and welfare and instruct veterinarians in the appropriate diagnosis and therapeutic management of uterine disease in this vulnerable species.

Spontaneous splenic rupture secondary to salmonella bacteraemia: a case report and review of literature

INTRODUCTION Spontaneous splenic rupture without any history of trauma is a rare and under-recognized entity in patients presenting with acute abdomen. This is a life threatening condition seen in association with various haematological malignancies, medications and blood stream infections. CASE PRESENTATION We report the case of a ninety-four-year-old male who presented to a regional Australian Hospital with abdominal pain, fever and hypotension. Imaging revealed splenic rupture. Salmonella Typhi was isolated on blood and peritoneal cultures. The patient’s Salmonella exposure was likely related to frequent consumption of raw eggs. He was managed conservatively with intravenous antibiotics to treat salmonella bacteraemia. Unfortunately, the patient died prior to discharge. DISCUSSION Salmonella bacteraemia is an uncommon cause of splenic rupture. Timely diagnosis and identification of the underlying aetiology of splenic rupture is essential for targeted management of such patients. CONCLUSION Spontaneous splenic rupture is a rare and potentially life threatening disorder which should be considered in the differential diagnosis of acute abdomen, regardless of any occurrence of trauma. Early recognition of this condition is essential to prevent the associated morbidity and mortality.

Probiotic Powder Supplementation in Haematology and Biochemistry Blood Late-Phase Laying Hens

Probiotic Powder Supplementation in Haematology and Biochemistry Blood Late-Phase Laying Hens

THE SUB-ACUTE TOXICITY OF THE DISINFECTIOUS PREPARATION "IODOPOVIDONE"

The results of a preclinical study of the subacute toxicity of the disinfectant "Iodopovidone" are presented. The purpose of the experiment reflected in the article was to continue a series of preclinical studies of the disinfectant Iodopovidone on laboratory animals by examining subacute toxicity, to confirm good animal tolerance and the drug belongs to the 4th toxicity class – low-toxic substances. The experiment was carried out on laboratory white rats, which were kept in the vivarium of the Faculty of Veterinary Medicine of the National University of Life and Environmental Sciences of Ukraine in compliance with the requirements of the European Convention for the Protection of Vertebrate Animals used for scientific experiments or for other scientific purposes and the Law of Ukraine "On the Protection of Animals". The subacute toxicity of the disinfectant "Iodopovidone" was studied using dermal application. The effect of the drug was evaluated by analyzing the change in biochemical parameters of blood serum and hematological parameters of the blood of laboratory animals in dynamics after 6, 24 and 48 hours. The results of determining the parameters of subacute toxicity when applied to the skin indicate the absence of an irritating effect of the drug on the skin. Under the influence of the drug on the organism of animals for six hours, a slight fluctuation in hematological and biochemical blood parameters was revealed, which approached those of the control group of animals within 48 hours, except for the level of glucose, but this did not affect the general condition of the animals. The use of the drug at a dose of 2900 mg/kg of body weight does not cause the death of animals, which confirms the low toxicity of the drug and belonging to the 4th class of toxicity according to the current sanitary and hygienic standards, which is promising for use in poultry farming and requires further studies of chronic toxicity and toxicity in inhalation use.

Весовой и линейный рост, гематологические показатели крови овец горьковской породы

The article considers the weight growth of sheep and eggs of the Gorky breed of sheep at birth, at weaning (4 months), at 6, 7 and 8.5 months; linear dimensions (body stats) were evaluated in sheep producers, and hematological blood parameters in lambs

Que tipo de lençol prefere o peixe dorminhoco gordo? Efeito da cor do fundo do tanque no crescimento e na saúde dos Dormitator latifrons (Richardson, 1844)

Abstract A little-studied characteristic of the Mexican native fish Dormitator latifrons is the effect that the color of the bottom or lining of ponds or tubs can have on their growth and blood parameters under controlled conditions. After a quarantine period in prophylactic treatment, an initial assay was performed. The organisms were grouped into four treatments (white, yellow, blue, and black) in triplicate, with 30 individuals with an average weight of 34.7± 2.5 g and average size of 12.5 ± 1.5 cm per tank. The trial lasted 60 days, after which a final biometry to all specimens and blood extractions to six random organisms per tank were performed. The following hematological and blood chemistry parameters were evaluated: erythrocytes, cell counts, and differential counts, as well as glucose, albumin, a/g ratio, and total proteins. Final weight, weight gain, and specific growth rate presented statistical differences between treatments (p&lt;0.05), with dark bottoms (blue and black) above 80% of WG and above 1.0 of SGR. No statistically differences were found in hematological blood chemistry parameters (p&gt;0.05). The growth results suggest that dark pond bottoms promote the adaptation of Dormitator latifrons by allowing it to avoid detection by predators through the adoption of a cryptic coloration. However, the species shows a great capacity for crypsis, being able to change its pigmentation to adapt to different bottom colors.

ВЛИЯНИЕ ВИДОСПЕЦИФИЧНОГО ПРЕПАРАТА ПРОСТИМУЛ НА УРОВЕНЬ ЭКСПРЕССИИ ГЕНА IL-1 НОВОРОЖДЕННЫХ ПОРОСЯТ, БОЛЬНЫХ КОЛИБАКТЕРИОЗОМ

The article presents the data on the expression level of the pro-inflammatory cytokine interleukin-1 (IL-1) in piglets 3-5 days of age diseased with colibacteriosis, against the background of the use of the species-specific drug Prostimul, containing recombinant type 1 cytokine, vitamins A, E and C, in comparison with antibacterial drug Quinocol Plus. The analysis of the obtained data has shown that the drug Prostimul suppresses the expression of IL-1β by 70 times, and Quinocol Plus – by 6.5 times. This fact indicates the prospect of using the drug to reduce the development of an inflammatory reaction in case of colibacteriosis in newborn piglets. When analyzing hematological blood indicators, a decrease in the number of stab neutrophils was revealed against the background of the use of Prostimul and Quinocol Plus. The analysis of leukocyte indices of blood intoxication has indicated that against the background of the use of the drug Prostimul this indicator decreases by 4.9 times, and Quinocol Plus – by 5.6 times, respectively.

Study of the mechanisms of combined pharmacological effects of anxiolytics, antioxidants and immunocorrectors in neurotic disorders

With the discovery of psychotropic diseases, it became possible to solve the problem of eliminating psychomotor agitation in patients, cases of disease, the development of behavioral disorders and the treatment of productive and negative psychopathological symptoms. But over time, unwanted antipsychotic drugs have been identified. One of the effects of such neuroleptic drugs is their effect on hematological blood parameters. Violation of hematological parameters can occur as a change in the number of formed elements, and the impact on the blood coagulation system