Blood Cell Membrane Stabilization Method Research Articles

Inhibitory effects of Anthocephalus cadamba stem bark fractions intercede anti-inflammatory and carbon tetrachloride induced hepatotoxicity in rats

Anthocephalus cadamba (Roxb.) Miq. (Rubiaceae) is commonly known as “Kadamba”, frequently found in moist deciduous evergreen forests. The present study was performed to evaluate the anti-inflammatory and hepatoprotective potential of A. cadamba fractions in relation to the free radical scavenging properties to corroborate and establish scientific evidence for its ethno-botanical uses. Quantification assay were determined for total phenolic and flavonoid compounds. Free radical scavenging properties were assessed by 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH), nitric oxide, peroxynitrite, superoxide, hypochlorous acid scavenging methods. Protein denaturation and human red blood cell membrane stabilization methods were performed to determine anti-inflammatory activity. Furthermore, Hepatocuretive potential was evaluated using carbon tetrachloride intoxicated (CCl4) rat’s model. Methanol fraction of A. cadamba (MEAC) was found to be the most potent free radical scavenger in all in vitro models. The higher inhibition properties of MEAC positively correlated with total phenolic (164.75 ± 3.83 mg GAE/g) and flavonoid compounds (51.20 ± 2.15 mg QE/g). The MEAC showed significant (p < 0.01) anti-inflammatory activity in all in-vitro methods. The A. cadamba significantly (p < 0.01) decreased levels of serum glutamic oxaloacetate transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase and total bilirubin in the treated groups when compare to CCl4 control group. Treatment with A. cadamba significantly (p < 0.01) increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 control group. These results indicated that A. cadamba possess anti-inflammatory and hepatoprotective property which may be mediated through augmentation of antioxidant defenses.

Evaluation of antinociceptive, anti-inflammatory and anxiolytic activities of methanolic extract of Terminalia citrina leaves

ObjectiveTo investigate the antinociceptive, anti-inflammatory and anxiolytic effects of methanolic extracts of Terminalia citrina (T. citrina) leaves (Family: Combretaceae). MethodsThe antinociceptive activity was evaluated by acetic acid induced writhing method and radiant heat tail flick method while anti-inflammatory activity was evaluated by human red blood cell membrane stabilization method and anxiolytic activity by elevated plus maze model. ResultsThe methanolic extract of T. citrina leaves showed significant antinociceptive, anti-inflammatory and anxiolytic effects in dose dependent manner compared to their respective standard drugs. ConclusionsThe present study demonstrated that T. citrina possesses antinociceptive, anti-inflammatory and anxiolytic effects.

In vitro and in vivo antiinflammatory activity of Clerodendrum paniculatum linn. leaves

Preliminary phytochemical screening showed the presence of terpenes, flavonoids, tannins, alkaloids, phenolic acid, sterols, and glycosides. This study was intended to evaluate the antiinflammatory activity of various extracts of fresh leaves of Clerodendrum paniculatum Linn experimentally by in vitro (human red blood cell membrane stabilization method) and in vivo methods (0.1 ml of 1% w/v carrageenan-induced rat paw oedema model). Petroleum ether, chloroform, ethyl acetate, alcohol, and aqueous extracts were screened for in vitro antiinflammatory activity. Petroleum ether and chloroform extracts which showed, best in vitro antiinflammatory activity was screened for in vivo antiinflammatory activity at the dose level of 200 and 400 mg/kg. Indomethacin at the dose level of 10 mg/kg was used as reference standard drug. Both the extracts showed a dose dependent significant (P<0.001) reduction in paw edema when compared to the control, at all the time intervals and comparable to indomethacin (reference standard) treated group. The results of the present study demonstrate that petroleum ether and chloroform extracts possess significant (P<0.001) antiinflammatory potential which provide scientific basis for the traditional claims of Clerodendrum paniculatum Linn leaves as an antiinflammatory drug.

Synthesis and biological evaluation of diamide derivatives of (S)-BINOL and biphenyl as potential anti-inflammatory/anti-arthritic agents

A series of (S)-BINOL and biphenyl based diamides have been synthesized and characterized from analytic and spectral data. Anti-arthritic activity was studied by inhibition of protein denaturation method (Bovine serum albumin). All the diamides exhibit excellent anti-arthritic activity. Anti-inflammatory activity of the diamides synthesized was investigated using human red blood cells membrane stabilization method, and some of the diamides exhibit good anti-inflammatory activity.

Anti–inflammatory activity of the glandular extracts of Thunnus alalunga

Objective To evaluate the anti-inflamattory activity of Thunnus alalunga by both in vitro and in vivo methods. Methods Anti-inflammatory activity of the chloroform water extract of Thunnus alalunga was done by both in vitro and in vivo methods. In vitro method was done by human red blood cells membrane stabilization method (HRBC). In vivo evaluation was estimated on Wister albino rats. Acute toxicity studies were done on the extract and no toxicity was reported. Results The percentage protection exhibited by 300 mg/mL concentration was more when compared to the other ones. The 400 mg/mL concentration showed potent activity on comparison with the standard during in vivo evaluation. Conclusions In both means of estimation the extract of Thunnus alalunga was found to possess significant anti-inflammatory activity.

In vitro and In vivo anti-inflammatory activity of leaves of Symplocos cochinchnensis (Lour) Moore ssp laurina

The anti-inflammatory activities of different extracts of leaves Symplocos cochinchinensis Lour ssp laurina (Symplococaceae) were investigated for i n vitro anti-inflammatory activity by human red blood cell membrane stabilization method. The methanol extract showed effective in vitro anti-inflammatory activity was screened for in vivo antiinflammatory activity by carrageenan-induced paw edema in rat model. The potency of the extracts was compared with standard diclofenac (50 mg/kg). The methanol extract showed significant membrane stabilizing action on human red blood cell membrane and reduction of edema in carrageenan induced rat paw edema model.