To determine the optimal method and timing to inseminate artificial oocytes produced by nuclear transfer of haploid pseudo-blastomeres (HpBs). Metaphase II (MII) oocytes were harvested from FVB/N mice and treated with 8% ethanol for parthenogenetic activation. Monopronucleated oocytes with extruded polar body (1PN/2PB) were cultured to the 8-cell stage and treated with a reversible microtubule polymerization inhibitor (nocodazole, 10 μM) to synchronize HpBs at the G2/M phase until nuclear transfer. Another cohort of B6D2F1 mouse oocytes were harvested, exposed to 5 μM cytochalasin B, and enucleated to generate donor ooplasts. A single HpB was coated with inactivated Sendai virus and subzonally transferred into the perivitelline space of the donor ooplast. Successfully reconstructed oocytes were either inseminated by conventional IVF immediately (0h-IVF) with C57BL/6 sperm or inseminated by piezo-actuated ICSI 2h (2h-ICSI) after nuclear transfer. Control zygotes were generated by piezo-ICSI of intact oocytes. All oocytes were cultured up to 96h in a time-lapse incubator to obtain and compare morphokinetic data for full preimplantation development. A total of 149 FVB/N oocytes were activated, and 29.5% reached the 8-cell stage 48h later, generating a total of 352 HpBs. Another cohort of 188 B2D2F1 oocytes were enucleated at a survival rate of 98.4%. Nuclear transfer of HpBs yielded a total of 167 oocytes, which were allocated to the 0h-IVF and 2h-ICSI groups. A portion of those allocated to 2h-ICSI spontaneously re-cleaved before ICSI insemination. While no oocyte was degenerated in the 0h-IVF group, the ICSI survival rate of the 2h-ICSI group was comparable to the control-ICSI group (63.0% vs. 67.7%). When comparing fertilization success, the 2h-ICSI group yielded a significantly lower rate (61.5%) than the 0h-IVF group (86.4%, p = 0.009). When comparing development rates to the blastocyst stage, the 2h-ICSI group yielded a rate of 7.7%, while the 0h-IVF group trended to a superior yield of 23.5%, albeit not statistically significant. When blastulation morphokinetic data were compared, all progressing embryos from the 0h-IVF, 2h-ICSI, and control groups showed comparable timing. To date, after transferring 22 morula/blastocysts from the 0h-IVF group, 1 pregnancy was generated, resulting in 3 healthy pups (2 male and 1 female). Our technique can reproduce functional copies of female gametes from a single ovum using a mouse model. While the spindle physiology and cell cycle of these artificial oocytes are not well understood, we determined that conventional IVF is an effective method for insemination, yielding a higher fertilization rate and trending to a higher blastocyst yield.