Abstract
Cyclic nucleotides cAMP and cGMP are among the main molecules that control the maturation of mammal oocytes. The in vitro simulated physiological oocyte maturation (SPOM) method was used to model cAMP accumulation in the oocyte. In the current study, we preincubated the oocytes of cows (not primed with FSH) with cAMP modulators: N 6,2’-O-dibutyryladenosine 3’,5’-cyclomonophosphate (dbcAMP) and 3-isobutyl-1-methylxanthine (IBMX). The use of SPOM increased the yield of bovine blastocysts.
Highlights
During maturation, mammal oocytes are retained in the meiosis diplotene
Oocyte-cumulus complexes were placed for 3 hours in a CSCC culture medium containing 1 mM N6,2ʹ-O-dibutyryladenosine 3',5'-cyclomonophosphate (Sigma) and 0.5 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma)
The oocytecumulus complexes were placed in a CSCC medium with 0.1 IU follicle-stimulating hormone (FSH) for 22 hours coated with paraffin Oil for Tissue Culture in 35 mm Petri dishes at 38.5 °C in a humid atmosphere with 6.5% CO2
Summary
Mammal oocytes are retained in the meiosis diplotene. At the same time, oocytes form specific large nuclei called germinal vesicles. The "pre-maturation in vitro" (preIVM) method, which simulates the natural physiological growth of cyclic nucleotides during follicle maturation, can increase the oocyte competence and, as a result, increase the number of good-quality embryos.
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