Black Cumin Seeds Research Articles

Black cumin Potential Anticancer effect of Black Cumin seed (Nigella sativa L.) extracts as determined by Cytotoxicity test against Larvae of Artemia salina Leach using Brine Shrimp Lethality Test (BSLT)

Black cumin plant seed or black seed (Nigella sativa L.) is among the most commonly used spices. This plant is grown mostly for its spicy seeds. Studies have reported that black cumin seeds have high levels of antioxidant that correlates with anticancer activities. The current study aims to determine the cytotoxic effect of black seed extracts on brine shrimp larvae (Artemia salina Leach) using Brine Shrimp Lethality Test (BSLT). Black cumin seed extract was obtained through percolation method using an extraction solvent (ethanol 96%). The cytotoxicity test was performed at 0 ppm concentration (control) and 10, 50, 100, 250 and 500 ppm concentrations of black seed extracts. We use 10 Brine Shrimp larvae per tube and repeated the test three times for each test group. Our results show that ethanolic extract (90%) of black cumin seeds (Nigella sativa L.) has a strong cytotoxic effect on Artemia salina larvae with the LC50 value of 107.2 ppm.

Utilization of Black cumin seed (<em>Nigella sativa </em>L.) fractions on quality characteristics of cookies

Black cumin (Nigella sativa L.) locally known as “Kalonji” is a good source of nutritionally essential components. Black cumin seeds have been used as herbal medicine by various cultures and civilizations to treat and prevent a number of diseases. Black cumin seed fixed oil contains appreciable quantities of unsaturated fatty acids, especially polyunsaturated fatty acids. In present investigation, the efforts were made to study the effect of incorporation of different levels of black cumin seeds fraction in preparation of cookies so as to enhance its nutritional, organoleptical and nutraceutical properties. The results revealed that black cumin fixed oil incorporation in cookies enhances the sensorial as well as physico-chemical properties of cookies up to standard level. The results are more promising in the samples containing black cumin fixed oil. The crude fibre and protein content of prepared cookies were present in high amount as well as total tocopherol content was in sufficient quantity.

Ethanol Extract of Black Cumin Seed (Nigella Sativa) Reduces Expression Kidney iNOS in Mice Model Preeclampsia

This study aims to analyze the effect of ethanol extract of black cumin seeds (Nigella Sativa) on iNOS expression in the kidney of mice model preeclampsia. Design This study uses an experimental design with a posttest only control group design. Using a mice model preeclampsia. This study was divided into 6 groups: negative control, positive control (preeclampsia model), model + black cumin seed extract 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day days, after surgery on mice then examined iNOS Kidney expression by methods Immunohistochemical. Data from observations were analyzed by one way ANOVA test. The result there is an effect of ethanol extract of black cumin seeds on renal iNOS expression in mice model preeclampsia (p

Ethanol Extract of Black Cumin Seed (Nigella Sativa) Reduces Expression Kidney iNOS in Mice Model Preeclampsia

This study aims to analyze the effect of ethanol extract of black cumin seeds (Nigella Sativa) on iNOS expression in the kidney of mice model preeclampsia. Design This study uses an experimental design with a posttest only control group design. Using a mice model preeclampsia. This study was divided into 6 groups: negative control, positive control (preeclampsia model), model + black cumin seed extract 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day days, after surgery on mice then examined iNOS Kidney expression by methods Immunohistochemical. Data from observations were analyzed by one way ANOVA test. The result there is an effect of ethanol extract of black cumin seeds on renal iNOS expression in mice model preeclampsia (p <0.05) at doses of 500mg, 1000mg, 1500mg, and 2000mg on decreasing renal iNOS expression. The conclusion Ethanol extract of black cumin seeds (Nigella sativa) can reduce iNOS Kidney expression in Mice Preeclampsia.

Ethanol Extract of Black Cumin Seed (Nigella Sativa) Reduces Expression Kidney iNOS in Mice Model Preeclampsia

This study aims to analyze the effect of ethanol extract of black cumin seeds (Nigella Sativa) on iNOS expression in the kidney of mice model preeclampsia. Design This study uses an experimental design with a posttest only control group design. Using a mice model preeclampsia. This study was divided into 6 groups: negative control, positive control (preeclampsia model), model + black cumin seed extract 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day days, after surgery on mice then examined iNOS Kidney expression by methods Immunohistochemical. Data from observations were analyzed by one way ANOVA test. The result there is an effect of ethanol extract of black cumin seeds on renal iNOS expression in mice model preeclampsia (p <0.05) at doses of 500mg, 1000mg, 1500mg, and 2000mg on decreasing renal iNOS expression. The conclusion Ethanol extract of black cumin seeds (Nigella sativa) can reduce iNOS Kidney expression in Mice Preeclampsia.

E4An Ultrastructural Study by Transmission Electron Microscope of Exocrine Pancreatic Cells in Diabetic Rats Treated with Herbal Combination

The current study was designed to investigate the alterations in the ultrastructure of orgenelles and cellular activity of exocrine pancreatic acini of experimentally induced-diabetic rats and to assess the usefulness of herbal combination supplementation in improving the ultrastructure and cellular activity of exocrine pancreas. The number of albino male rats used were 24 which divided into equally 4 groups; group I: control group, group II: alloxan-induced diabetes mellitus (single intraperitoneal dose of alloxan 120 mg/kg for 3 days), group III: herbal combination treatment composed from the extracts of fenugreek seeds (Trigonella foenum-graecum), black cumin (Nigella sativa) seeds, rhizomes of ginger (Zingiber officinale), leaves of olive (Olea europeae), and seeds of ash (Fraxinus ssp). Each rat given 2.5 ml (0.5 ml from each five mixed plant extracts used) for 2 months. group IV: diabetes was induced as group II and treated with herbal combination as group III for 2 months. The processing for investigating by transmission electron microscopy were carried out for all pancreata taken from all groups. There was significant reduction (p&lt;0.05) in mean of FBG levels in diabetic rats treated with herbal combination (group IV) as compared to diabetic control and could revert FBG to normal value compared to negative control The results of examination of semithin and ultrathin sections in diabetic rats revealed many degenerative changes in the exocrine pancreatic tissue in comparison to control group. These degenerative changes can be summarized as disturbances in the arrangement of pancreatic acinar cells and decreased secretory granules in addition to the vacuolation of cytoplasm and pyknotic nuclei were observed in semithin sections. Moreover, degenerated nuclei, vacuolation of cytoplasm, fragmentation of rough endoplasm reticulum and degranulation of the most pancreatic acinar cells were noticed in ultrathin sections. In contrast, the acinar cells of group administrated with herbal combination only had normal ultrastructure, cellular activity, and nuclei, well-developed rough endoplasmic reticulum with abundant zymogen granules. Also, most of the acinar cells retrieved their normal ultrastructure and cellular activity in diabetic group treated with herbal combination and herbal combination could decrease most of the degenerative changes caused by alloxan-induced diabetes. In conclusion, the daily supplementation with this herbal combination to diabetic rat for 2 months could achieving promising effects due to its cytoprotective influence and showed the ability to decreased degenerative damage in the ultrastructure and cellular activity of exocrine pancreatic acini in type 2 diabetic rats.

Biosorption of Copper by a Natural Byproduct Material: Pressed Black Cumin Cakes

A natural waste material, pressed black cumin cake, was characterized as an adsorbent for copper (II) ions. The pressed black cumin cake waste was obtained after the cold pressing of black cumin seeds in order to extract black cumin oil. The press cake was used as an adsorbent after washing with cold water for the removal of copper (II) ions. The effects of pH and adsorbent dose on the removal efficiencies were investigated. The point of the zero charge was determined. Isotherm studies were performed using Langmuir, Freundlich, and Dubinin-Radushkevich models. The adsorption of the copper (II) was found to comply with the Langmuir isotherm model. The maximum adsorption capacity calculated from the Langmuir isotherm was found to be 106.38 mg/g. Thermodynamic studies were also carried out. Furthermore, three kinetic models, the pseudo first-order, pseudo second-order, and intraparticle diffusion, were investigated. The adsorption adhered to the pseudo second order kinetic model. The regeneration of the adsorbent was also investigated. An environmentally friendly, low cost, fast, and easily accessible adsorbent was developed for the efficient removal of copper (II). It is advantageous in terms of stability for long-term use.

Black Cumin Seeds Extract Increase Lymphocyte Activity in IFN-γ Secretion in Sprague Dawley Rat (SD) Induced by Dimethylbenzantracene

Interferon-gamma (IFN-γ) is one of the central cytokines in the anti-carcinogenesis immune response. Black cumin seeds (BCS) have an active content of thymoquinone and unsaturated fatty acids with biological activity as immunomodulators. This study aimed to determine the effect of administration of BCS extract on IFN-γ secretion activity by DMBA-induced SD rat lymphocytes. In vivo experimental study on DMBA-induced SD rats, BCS extract was given with three doses for two weeks before being induced and five weeks during DMBA induction. IFN-γ levels in lymphocyte culture supernatants were determined by the ELISA method. The difference in IFN-γ levels between groups was analyzed by ANOVA test, the significance of 95%. The results showed that administration of BCS extract for 14 days did not affect cellular composition toward the edge of the test animal. BCS extract can increase IFN-γ secretion activity by DMBA-induced SD rat lymphocytes.Keywords: black cumin seed, IFN-γ; DMBA: immunomodulator, carcinogenesis.

A Review of the Seeds Comprising Panch phoron, a Spice used in Indian Cuisine

India houses a wide variety of plants with medicinal properties on account of being a megadiversity country. Spices, comprising different parts of a plant (seeds, bark, etc.), are substances used for adding flavor to and preserving food items. They possess a myriad of health benefits, uses of which are documented in ancient literature. Panch phoron is a mixture of five spices, comprising whole seeds of cumin, fennel, fenugreek, mustard and black cumin. It is widely used in India, especially Eastern India, for adding flavor to food in daily cooking. The present study delves into various pharmacological properties of each of the five seeds comprising Panch phoron and is necessitated by the current interest in plant products as a cheaper and far better alternative than the synthetic drugs available but associated with side effects. It was seen that the seeds have myriad properties such as antimicrobial, antioxidant, antitumorigenic, anti-inflammatory, antidiabetic, gastroprotective and cardioprotective and may be used for the treatment of diabetes, cancer, inflammation, kidney problems, liver problems, etc., following proper scientific validation and long-term trials. The review aspires to scientifically validate the vast traditional use of this spice in the Eastern Indian cuisine and its immense scope in herbal therapeutics in the future.

Effect of Supplementation of Ginger Root Powder and Black Cumin Seed Powder on Performance of White Leghorn Layers

Effect of Supplementation of Ginger Root Powder and Black Cumin Seed Powder on Performance of White Leghorn Layers

ENHANCING THE GROWTH AND PRODUCTION OF BLACK CUMIN (NIGELLA SATIVA L.) BY APPLICATION OF HUMIC ACID AND BIOFERTILIZERS

The safe production (chemical-free) of medicinal and aromatic plants has been the focus of interest of various researchers worldwide. Therefore, the aim of this experiment was to study the effect of humic acid (HA) and some bio-fertilizers on growth, seed yield and oil content of black cumin (Nigella sativa L.). Plants were foliar sprayed with HA at 100, 200 and 400 mg/L while control plants were sprayed with tap water. The bio-fertilizers used in this study were nitrogen fixing bacteria (Bio 1) that consists of Azotobacter chroococcum + Azospirillum brasilense, phosphate solubilizing bacteria (Bio 2) which consists of Bacillus megatherium var. phosphaticum + Bacillus polymyxa and the mixture of (Bio 1 + Bio 2) as a combination treatment. Plants were not received any bio-fertilizers were set as a control. The growth characters (plant height, branch number and fresh and dry weight per plant), seed yield components (capsule number, seed yield per plant and fed.) were significantly enhanced due to HA and/or biofertilizers treatments relative to the control. In all investigated parameters, the combined treatment of two biofertilizers (Bio 1 + Bio 2) was superior to solely application. Applying HA at 400 mg/L had no impact on growth and yield compared with 200 mg/L. The highest seed yield was recorded by the combined treatment of HA at 200 mg/L and the mixture of Bio 1 + Bio 2. The volatile oil percentage and yield were also improved by applying HA and/or biofertilizers treatments and the same trend was observed for fixed oil as well. Moreover, the GC-MS analysis of volatile oil indicated that the main constituents were also affected by HA and/or biofertilizers applications. Generally, the combination between HA and the mixture of biofertilizers increased the percentages of the main components compared to untreated plants. Furthermore, the N, P, K, total chlorophyll and carbohydrates were significantly increased due to HA and/or biofertilizer treatments relative to the control. To reduce the use of chemical fertilizers, minimize the production cost, improve soil structure, increase the quality of black cumin seeds and oils, the combined treatment of HA at 200 mg/L and the mixture of Bio 1 + Bio2 was recommended.

Nigella sativa seed based nanohybrid composite-Fe2O3–SnO2/BC: A novel material for enhanced adsorptive removal of methylene blue from water

In this work, an advance approach is reported for the water treatment technology using nanohybrid composite Fe2O3–SnO2/BC prepared by incorporation of iron-tin binary oxide into the cellulosic framework of medicinally active Nigella sativa (Black cumin) seed powder. The co-precipitation method was followed to prepare the nanohybrid composite which was subjected to investigate its physiochemical properties using spectroscopic and microscopic techniques. Fourier-transform infrared spectroscopy analysis confirmed the formation of highly functionalized nanocomposite through the hydrogen and electrostatic interactions between the functional groups of seeds and Fe2O3–SnO2. X-ray and selected area electron diffraction pattern revealed the presence of cubic phase of γ-Fe2O3 and tetragonal phase of SnO2 in the composite. The scanning electron microscopic images suggested the porous and relatively smooth surface of the composite, and transmittance electron microscopic images showed the trapping of nano-cubes of Fe2O3–SnO2, having particles size in the range 95–185 nm, into the organic framework of Black cumin seeds, whose zero point charge was found at pH 7.2. The composite was investigated for adsorption of Methylene blue dye from water for which the results revealed that 2.0 gL-1 amount of Fe2O3–SnO2/BC was sufficient to remove more than 95% dye, within 15 min, at 6–9 pH, from its 10 mgL−1 concentration. The thermodynamic studies established spontaneity, feasibility, and endothermic nature of the adsorption process. The adsorption data was satisfactorily described by the Freundlich isotherm which indicated inhomogeneous surface of the composite. Application of Temkin isotherm revealed the same extent of bonding probability and heat of adsorption at 27, 35, and 45 °C. The free energy change calculated from Dubinin-Radushkevich isotherm suggested weak interaction between Methylene blue and Fe2O3–SnO2/BC. The process satisfactorily followed the pseudo-second order kinetics that was controlled by the film diffusion step which indicated interaction of Methylene blue with functional sites of the Fe2O3–SnO2/BC. The Fourier-transform infrared spectroscopy analysis gave the confirmatory evidence for interaction of Methylene blue to Fe2O3–SnO2/BC. The maximum Langmuir adsorption capacity of the Fe2O3–SnO2/BC was found to be 58.82 mgg−1 at 27 °C which is higher than the previously reported adsorbents, MnFe2O4/BC [J. Clean. Prod. 2018. 200, 996–1008], and Fe2O3–ZrO2/BC [J. Clean. Prod. 2019. 223, 849–868]. Therefore, the study showed excellent results for water treatment and can be useful to develop advance water treatment technology.

Oil extraction from blends of sunflower and black cumin seeds by cold press and evaluation of its physicochemical properties

Oil extraction from blends of sunflower and black cumin seeds by cold press and evaluation of its physicochemical properties

Comparative essential oil composition and fatty acid profiling of some Iranian black cumin landraces

Seeds of black cumin have high values of thymoquinone in essence. Metabolites of black cumin seeds would present a potent and therapeutically activity on the cardiovascular, respiratory, immune, and endocrine systems. Therefore, it is worthwhile to find superior landraces of black cumin, for further breeding objects. In this study, 16 landraces were collected from different regions of Iran and then were cultivated in a same climate condition in a randomized complete block design (RCBD) with three replications for two years (2015–2016). Fixed oil content of seeds was completely extracted by petroleum ether assisted soxhlet extraction. In addition, the essential oil was obtained by hydrodistillation from both fixed oil and seeds. Essential oil composition and fatty acid profiling were identified by GC/FID and GC/MS. Results showed that the highest content of essential oil were observed in Gerdmiran (1.28%) and Khaaf (1.25%) landraces. Thymoquinone and p-cymene (70–79%) were the main compounds of the essential oil; so that the highest amount of thymoquinone were recorded in both years in Esfehan (40.15% and 44.77% for the first and second year respectively) landrace. The highest content of fixed oil was observed in Sarayan landrace (33.04%). Generally, 86% to 93% of fatty acids were comprised of three main compounds including linoleic acid (omega 6), oleic acid and palmitic acid. The highest amount of linoleic acid obtained in landraces of Bolbanabad (55.95%) in first year and Bajestan (54.78%) in second year. Highest amounts of oleic acid and palmitic acid were observed in Sarayan (29.39% and 28.09%) and Komme (12.47% and 12.83%) landraces in both years.

Simultaneous adsorptive study of toxic metal ions in quaternary system from aqueous solution using low cost black cumin seeds (Nigella sativa) adsorbents

A new adsorbent obtained from alkaline treatment of black cumin seeds (BCS) has been developed for the adsorption of cation pollutants cobalt [Co(II), lead Pb(II), copper Cu(II) and nickel Ni(II)] from aqueous solutions. The alkaline treated black cumin seeds were labeled; base treated black cumin seeds (BT-BCS), acid treated black cumin seeds (AT-BCS) and untreated black cumin seeds (UT-BCS). The absorbents were analyzed by scanning electron microscope (SEM), fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction spectroscopy (XRD). The SEM images showed that UT-BCS is composed of spherical shaped particles. AT-BCS and BT-BCS images showed irregular surface morphology. AT-BCS and BT-BCS –IR spectra observed new bands. Pseudo First Order kinetic (PFO) model gave consistent fit in describing the adsorption of all the cations. The PFO good fit suggest that the adsorption mechanism involved, Van der Waal forces. The estimated pore adsorption (EPA) was more dominant than estimated surface adsorption (ESA). Equilibrium data had a good fit for Langmuir isotherm model for all cations this indicates that adsorption took place on active sites having equal affinity for these ions and further suggests that monolayer adsorption occurred on adsorbents surface at the end of adsorption process. BT-BCS was the best performing adsorbent with capacity of 190.7 mg/g for Cu(II), AT-BCS and UT-BCS with capacities of 180.1 and 135 mg/g respectively for Pb(II). Adsorption trend is BT-BCS > AT-BCS > UT-BCS. Reusability data suggests that (BCS) adsorbents retained ≥ 90% of their initial cations adsorption, these adsorbents may be reusable in wastewater treatment.

Identification of Novel Proteins from Black Cumin Seed Meals Based on 2D Gel Electrophoresis and MALDI-TOF/TOF-MS Analysis.

The amount of cold press oil manufacture is globally rising, which in turn leads to the accumulation of deoiled plant seeds at significant quantities and consequent manufacture of plant protein products. In this study, we made an attempt to analyze the protein profile of black cumin seed protein concentrates prepared by the alkali extraction-acid precipitation technique (AE-IP). The analytical strategy relied on gel-based proteome mapping which included two-dimensional gel electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF/TOF). 14 different protein bands were identified, and in gel-trypsinolysis was carried out for the corresponding gel spots. Using the MASCOT database, current findings on 10 proteins were compared with the existing data. The highest similarity was 46 which was obtained between the highest pI black cumin protein observed here and the cyclin dependent kinase inhibitor of Arabidopsis thaliana. The molecular mass of the intact protein was determined by linear MALDI-TOF/TOF-MS as 23,711.2186Da. The peptide constructs of this protein have been further studied in order to identify potential biological activity. Matching sequences generated bioactive peptides in silico such as IR, AL, and SL dipeptides during sequential enzymatic digestion with pepsin and trypsin. Since the majority of bioactivity investigations on black cumin seeds have been related to black cumin oil and its oil soluble components, the structure and bioactivities of black cumin proteins deserve further research.

Reverse Screening Bioinformatics Approach to Identify Potential Anti Breast Cancer Targets Using Thymoquinone from Neutraceuticals Black Cumin Oil.

Functional foods, neutraceuticals and natural antioxidants have established their potential roles in the protection of human health and diseases. Thymoquinone (TQ), the main bioactive component of Nigella sativa seeds (black cumin seeds), a plant derived neutraceutical was used by ancient Egyptians because of their ability to cure a variety of health conditions and used as a dietary food supplement. Owing to its multi targeting nature, TQ interferes with a wide range of tumorigenic processes and counteracts carcinogenesis, malignant growth, invasion, migration, and angiogenesis. Additionally, TQ can specifically sensitize tumor cells towards conventional cancer treatments (e.g., radiotherapy, chemotherapy, and immunotherapy) and simultaneously minimize therapy-associated toxic effects in normal cells besides being cost effective and safe. TQ was found to play a protective role when given along with chemotherapeutic agents to normal cells. In the present study, reverse in silico docking approach was used to search for potential molecular targets for cancer therapy. Various metastatic and apoptotic targets were docked with the target ligand. TQ was also tested for its anticancer activities for its ability to cause cell death, arrest cell cycle and ability to inhibit PARP gene expression. In silico docking studies showed that TQ effectively docked metastatic targets MMPs and other apoptotic and cell proliferation targets EGFR. They were able to bring about cell death mediated by apoptosis, cell cycle arrest in the late apoptotic stage and induce DNA damage too. TQ effectively down regulated PARP gene expression which can lead to enhanced cancer cell death. Thymoquinone a neutraceutical can be employed as a new therapeutic agent to target triple negative breast cancer which is otherwise difficult to treat as there are no receptors on them. Can be employed along with standard chemotherapeutic drugs to treat breast cancer as a combinatorial therapy.

Impacts of supplementing broiler diets with a powder mixture of black cumin, Moringa and chicory seeds

The present study aimed at evaluating the effect of a phytogenic mixture in the diet on broiler production. A total of 400 day-old unsexed Cobb broiler chicks were randomly allotted to four treatment groups of 10 replications in a randomised design experiment. The phytogenic feed mixture (BMC) contained equal ratios of black cumin, Moringa oleifera and chicory seeds. The treatment groups were as follows: T1 was fed the basal diet, while T2, T3 and T4 were fed the basal diet supplemented with 0.2%, 0.4% or 0.6% of three BMC mixture, respectively. Results showed that increasing the dietary BMC level could be associated with a gradual but significant increase in body weight and improvement in the feed conversion ratio when compared with the control group. Broiler diets enriched with 0.4% to 0.6% of the BMC mixture reduced gut microbial count of coliforms, E. coli and C. perfringens as well as gut pH, compared to the control group. Increasing the dietary BMC mixture level was associated with gradual but significant decrease in serum total cholesterol, low density lipoprotein concentrations and liver enzymes concentrations. However, there was an increase in the high density lipoprotein concentration, and glutathione peroxidase and superoxide dismutase activity in serum. In conclusion, the BMC mixture could be deemed an effective growth promoter, but further research is needed to evaluate it as a viable alternative to antibiotics.Keywords: blood, broiler, carcass, microbiota, oxidative status, performance, phytogenic

Evaluating the role of microwave-baking and fennel (Foeniculum vulgare L.)/nigella (Nigella sativa L.) on acrylamide growth and antioxidants potential in biscuits

This study aimed to investigate the impact of fennel seeds (FS) and black cumin seeds (BS) addition into the formulation of conventional and microwave-baked biscuits on the nutritional, physical, and sensory properties. Microwave biscuits were baked at 700 W for 90 s. Antioxidant activity DPPH·, ABTS·+, and total phenolic compounds (TPC) were evaluated by using a UNICO UV-2100 spectrophotometer, acrylamide content was evaluated using UPLC–ESI–MS/MS. The results indicated that microwave-baked biscuits have higher antioxidant activities DPPH·, ABTS·+, TPC, moisture, and breaking strength and lower acrylamide content compared to conventional-baked biscuits. However, the addition of BS gradually decreased the acrylamide content from 355.2 µg/kg in control samples to 138.6 µg/kg in conventional-baked biscuits and from 306.9 µg/kg in control samples to 97.8 µg/kg in microwave-baked ones. Meanwhile, with FS biscuits, acrylamide is decreased to the minimum limit of the quantitation in microwave-baked biscuits and 38%, 61%, and 78% in conventional-baked ones at 2%, 4%, 6% FS respectively. Correspondingly, FS samples had higher antioxidant activities in DPPH·, ABTS·+ and TPC. Acrylamide was correlated to ABTS·+, DPPH·, and colour values on high negative levels (r = − 0.914, − 0.943, − 0.947 and − 0.943) at P < 0.01. High antioxidants activity and low acrylamide content indicated the advantages of microwave-baking and addition of FS or BS to biscuit formulation and they could be used in food industries as a potential plant source antioxidant as well as enhancing the sensory properties of the biscuits.

Impact of infrared and dry air roasting on the oxidative stability, fatty acid composition, Maillard reaction products and other chemical properties of black cumin (Nigella sativa L.) seed oil

Black cumin seed (BCS) is a novel oil source with potential health benefits. This study investigates the influence of infrared (IR) and dry air (DA) roasting (140, 160 and 180 °C for 5 and 10 min) on BCS oil quality characteristics. Results revealed that the oxidative stability index (OSI), Maillard reaction products (MRPs), chlorophyll and carotenoid contents were increased while acid value (AV), peroxide value (PV) and color values were decreased in DA roasted (180°C for 10 min) BCS oil compared to other DA and IR treatments. DA and IR roasting slightly influenced the fatty acid composition (FAC) of BCS oils. FTIR spectra showed minor changes in peak intensities (at 2854, 2929 and 3008 cm−1) of DA and IR roasted BCS oils. DA roasting proved more effective than IR roasting. The oil from the DA roasted BCS at 180°C for 10 min had significantly higher oil quality and oxidative stability.