Agroforestry (AF) effectively enhances ecological restoration and soil–water conservation (SWC), yet the relationship among soil and water conservation agroforestry (SWCAF) in karst soil, water loss (SWL) and rural eco-revitalization (RER) remains unclear, which may hinder the ecological restoration process around the world. This study aims to reveal whether SWCAF in karst areas improves RER through SWC benefits, ecosystem service (ES) enhancement and rural ecological environment quality (REEQ) improvement. We take Guizhou Province, the core area of the South China Karst (SCK), as the study area and 2010–2020 as the study period. By using the equivalent factor method, the remote sensing ecological index (RSEI) model, bivariate spatial autocorrelation and the panel vector autoregressive (PVAR) model, the study reveals SWCAF’s ecological benefits and its interaction mechanism with RER. Key findings reveal the following: (1) SWCAF reduced the area of SWL by 14.93% by converting cropland into forests. (2) The AF ecosystem service value (AFESV) increased by CNY 9.181 billion, and the forest-related AFESV increases represented 184% of the total AFESV, while REEQ showed an overall positive trend in the western SWC area. (3) The AFESV has an obvious synergistic effect with REEQ (r = 0.60) and obvious positive synergy with SWL (r = 0.69), and its spatial correlation increases over time. (4) The PVAR model verified that there is a bidirectional Granger causal relationship between the AFESV and RER, showing dynamic positive and negative alternating influences. This research study reveals that SWCAF drives RER through the dual path of SWL control and value-added ecological services, among which the forest ecosystem plays a core role. In the future, it is necessary to optimize the diversity of AF structures to avoid ecological service trade-offs. This research study provides a scientific basis for decision making and the ecological management of SWC in karst soils globally.
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