Bitter Flavor Research Articles

Identification and fungicide control of Bipolaris sorokiniana causing leaf spot and blight on common hop (Humulus lupulus) in Korea.

Hop (Humulus lupulus) is a perennial herbaceous vine belonging to the family Cannabeaceae. This crop is commercially grown for the brewing industry for its bitter and aromatic flavor, as well as its antiseptic properties. In June 2021, leaf spot and blight was observed on common hop plants in Buan-gun, Jeollabuk-do, South Korea. The typical symptoms were small to large, dark brown necrotic lesions with yellow halos on the leaves. This study aimed to clarify the causal agent of this disease. Two fungal species, Alternaria alternata and Bipolaris sorokiniana, were isolated from the diseased leaf samples and identified by combining morphological observations and phylogenetic analysis using sequence datasets of ITS, Alt a1, rpb2, endoPG, OPA10-2, and ITS, gpd, and tef1, respectively. Pathogenicity of the fungal isolates on detached leaves and living plants revealed that B. sorokiniana is the causal pathogen of this disease, while A. alternata is potentially a saprophyte. Fungicide sensitivity of the pathogen B. sorokiniana was further estimated in vitro using three classes of fungicides represented by fluxapyroxad, pyraclostrobin, and hexaconazole. The effective concentrations that inhibited 50% of spore germination (EC50) were 0.72 µg ml-1, 1.90 µg ml-1, and 0.68 µg ml-1, respectively. Moreover, all of these fungicides were able to control B. sorokiniana on detached common hop leaves at their recommended concentrations. In conclusion, this study reports leaf spot and blight of common hop caused by B. sorokiniana for the first time and proposes potential fungicides for this disease.

Momordica charantia (bitter gourd), a potent antidiabetic phytomedicine

Gourd bitter Grown mostly in India, China, and Southeast Asia, the tropical creeping plant known as or bitter melon. Its edible fruit is the primary reason for the plant's cultivation. Because of their bitter flavour, bitter gourds are not well-liked. The fruits of the Cucurbitaceae family plant Momordica charantia are a good source of a number of important nutrients. Over sixty phytomedicines, including blood purifiers, antioxidants, anticancer agents, and diabetes inhibitors, are present in the entire plant. In-depth information about the nutritional elements, different bioactive substances, and significant nutraceutical qualities of the bitter gourd plant in relation to diabetes are attempted to be provided in this review.

Contribution of Additional Glutamic Acid and Fructose in The Formation of Flavor Compounds in Green Tea

Green tea in Indonesia has an astringent and bitter taste due to its high catechin content. Adding amino acids and reducing sugars in green tea processing will increase the desirable flavor and cover the astringent and bitter flavors by increasing the Maillard reaction. Research on the manufacture of green tea with the addition of glutamate and fructose in fresh tea leaves has never been explored. This study aims to find a combination of glutamic acid (0%, 0.35%, 0.7%, 1.05%, 1.4%) and fructose (0%, 0.13%, 0.26%, 0.39%, 0.52%) to improve the flavor profile in green tea. The samples were evaluated for their antioxidant activity, amino acid, volatile, sensory evaluation, and color analysis. The results showed that adding glutamate and fructose increased the antioxidant activity of amino acid compounds and decreased the catechin content in green tea. Thus, the best treatment obtained was glutamate (1.05%) and fructose (0.26%), which increased the Maillard reaction in the formation of flavor compounds in green tea.

Ethnoscience Phenomenon: The Chemical Element Glucose as a Symbol of Human Enjoyment of Life in The Nyadran Celebration in Magelang

The purpose of this study is to determine the symbolic meaning represented by the food that must be present in every nyadran performance in Magelang City. This study is a case study with ethnographic approach. With the guidance of symbolic interactionism theory, the meaning of the taste of the chemical elements of the food will be analyzed so that there is a scientific explanation present. The informants in this study are artists, art observers and nutritionists. The results of the study, ethnoscience representations appear when the previous artists even though they cannot parse the chemical elements of coffee that create bitterness. Likewise, in the chemical elements of bananas that cause sweetness. Bitter is a flavor that in chemistry is caused by the compound trigonelline (niacin), which is found in coffee. Sweetness is a flavor that, according to chemistry, is caused by glucose in bananas. According to the informants, the sweet and bitter flavors in the food that must be present at the event represent how human life is always filled with two dominant feelings, namely bitter which is a representative of sadness, difficulty, suffering and sweetness which represents pleasure, happiness.

Trained sensory descriptors and volatile aroma compounds of USDA Select steaks using five grill temperatures

Thirty-three USDA Select boneless top loins were selected from carcasses at a commercial major packing plant, vacuum-packaged, and aged for 14 d (4 °C). The loins were then divided into 10 portions (5 grill temperatures for each of trained sensory panel and Warner-Bratzler shear force). Flat-top electric grills were pre-heated to 1 of 5 different temperatures: 149 °C (149), 177 °C (177), 204 °C (204), 232 °C (232), or 260 °C (260). Steaks were placed on the grill, turned when the internal temperature reached 35 °C and removed when the internal temperature reached 71 °C. A trained sensory panel evaluated ten basic flavors and five texture attributes. Extra cubes from each sample were frozen in liquid nitrogen and stored at −80 °C for GC/MS volatile aroma compound analysis. Beef identity, brown, and roasted flavor descriptors increased linearly (P < 0.001) while bloody/serumy tended to decrease (P = 0.016) and sour flavor decreased (P = 0.006) linearly as grill temperature increased. Furthermore, burnt (deviation P = 0.008) and bitter (deviation P = 0.012) flavor descriptors were affected by effects other than linear or quadratic, while umami (P = 0.002) and overall sweet (P = 0.016) flavors increased quadratically from 149 to 232 then declined at 260 grill temperatures. Two alcohols, eight aldehydes, four alkanes, three furans, eight ketones, and twelve pyrazines were impacted by differences the grill temperature. Increasing grill temperature increases volatile compounds, primarily from the Maillard reaction, that improve positive beef flavor descriptors.

Examining the Naringin Content and Sensory Characteristics of Functional Chocolate Fortified with Grapefruit Peel Extract

Grapefruit peel contains a high concentration of naringin- a potent antioxidant with strong bioactive properties. In this study, a new type of functional chocolate fortified with grapefruit peel extract and different concentrations of aqueous methanol and ethanol were evaluated as extraction solvents. A new high-performance liquid chromatography (HPLC) method to analyze the naringin content of the fortified chocolates was developed with a recovery of 107% ± 3.1% and repeatability below 3.5%. A sensory evaluation was conducted to assess the preference for the chocolates among individuals who self-described a preference for bitter flavors. No significant preference was observed in the cases of astringency and aftertaste while the increased bitterness proved to be favorable. However, taste, flavor and overall acceptability were regarded somewhat less favorably. While chocolate proved to be a satisfactory carrier for naringin and had several enjoyable characteristics, further research may focus on improving the organoleptic properties of chocolates fortified by naringin.

Modeling the antimicrobial effects of olive mill waste extract, rich in hydroxytyrosol, on the growth of lactic acid bacteria using response surface methodology.

The objective of this study is to assess the inhibitory effects of an aqueous extract from olive oil mill waste (alperujo) on the growth of a lactic acid bacteria (LAB) cocktail consisting of various strains of Lactiplantibacillus pentosus and Lactiplantibacillus plantarum species. For this purpose, response surface methodology was employed using two independent variables (pH levels 3.5-5.55; hydroxytyrosol concentration ranging from 0.93-2990ppm). The response variable was the average inhibition per treatment on the LAB cocktail (expressed as a percentage). The developed model identified significant terms, including the linear effect of hydroxytyrosol and pH, their interaction, and the quadratic effect of pH. Maximum inhibition of the LAB cocktail was observed at progressively higher concentrations of hydroxytyrosol and lower pH values. Therefore, complete inhibition of LAB in the synthetic culture medium could only be achieved for concentrations of 2984ppm hydroxytyrosol at a pH of 3.95. These findings suggest that extracts derived from "alperujo" could be utilized as a natural preservative in acidified foods with a bitter flavor and antioxidant requirements.

Profiling the Major Aroma-Active Compounds of Microwave-Dried Jujube Slices through Molecular Sensory Science Approaches.

To discriminate the aroma-active compounds in dried jujube slices through microwave-dried treatments and understand their sensory attributes, odor activity value (OAV) and detection frequency analysis (DFA) combined with sensory analysis and analyzed through partial least squares regression analysis (PLSR) were used collaboratively. A total of 21 major aromatic active compounds were identified, among which 4-hexanolide, 4-cyclopentene-1,3-dione, 5-methyl-2(5H)-furanone, 4-hydroxy-2,5-dimethyl-3(2H)furanone, 3,5-dihydroxy-2-methyl-4-pyrone were first confirmed as aromatic compounds of jujube. Sensory evaluation revealed that the major characteristic aromas of dried jujube slices were caramel flavor, roasted sweet flavor, and bitter and burnt flavors. The PLSR results showed that certain compounds were related to specific taste attributes. 2,3-butanedione and acetoin had a significant positive correlation with the roasted sweet attribute. On the other hand, γ-butyrolactone, 4-cyclopentene-1,3-dione, and 4-hydroxy-2,5-dimethyl-3(2H)furanone had a significant positive impact on the caramel attributes. For the bitter attribute, 2-acetylfuran and 5-methyl-2(5H)-furanone were positively correlated. Regarding the burnt flavor, 5-methyl-2-furancarboxaldehyde and 3,5-dihydroxy-2-methyl-4-pyrone were the most influential odor-active compounds. Finally, 2-furanmethanol and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one were identified as the primary sources of the burnt and bitter flavors. Importantly, this work could provide a theoretical basis for aroma control during dried jujube slices processing.

Investigation on consumer perception and acceptance of wheat beer using sorted napping and rate‐all‐that‐apply (RATA) questions

SummaryThis study aimed to investigate consumer perceptions and acceptance of wheat beer products. Twelve samples were evaluated using the sorted napping (SN) (n = 44) to classify them based on similarity, and the samples were divided into six groups. Based on the SN results, six representative samples with different attributes were used for further consumer testing. The consumers (n = 102) rated the likings, and the intensities of the attributes were evaluated using rate‐all‐that‐apply (RATA) questions. Although the six samples were classified into different segments by SN, no difference was found in consumer acceptance. For the segmentation of consumer groups by their liking patterns of the samples, the consumers were divided into three clusters. Interestingly, the intensities of most of the RATA terms did not differ by cluster; however, the intensities of very few RATA terms were evaluated differently by cluster. In particular, S6 in Cluster 3, which had the lowest acceptability among the samples, was relatively high in bitterness and alcohol flavour. Characteristics such as bitterness and alcohol flavour, which can be affected by personal traits (e.g. sensitivity to bitterness), were related to the drivers of dislike for wheat beer products.

New Coagulant Proteases for Cheesemaking from Leaves and Latex of the Spontaneous Plant Pergularia tomentosa: Biochemical Characterization of Coagulants and Sensorial Evaluation of Cheese

The purpose of this study was to evaluate the caseinolytic and milk-clotting activities of aqueous crude extracts from leaves and latex of the Pergularia tomentosa, to determine their suitability as a rennet substitute. These extracts were subjected to a series of biochemical tests before being used in the production of cheese. The results showed that the enzymatic latex extract had a higher coagulant activity than the leaf extract. However, under different clotting conditions (pH, temperature, and CaCl2 concentration), both coagulants behaved similarly in the coagulation of Berridge substrate. The SDS-PAGE and zymographic analysis revealed identical protein bands with a single active zone in both extracts, corresponding to a molecular weight of 26.98 kDa and 26.03 kDa in the extract of leaf and latex, respectively. Both extracts were stable to different effectors but strongly inhibited by iodoacetamide and Hg, suggesting it to be a cysteine protease. Both extracts were able to hydrolyze casein and generate peptides of 14 kDa, with excessive hydrolysis of the other casein fractions. The physicochemical parameters of cheese made from latex and leaf extract evolved similarly to control cheese. According to the sensory evaluation, cheese made with latex had a mildly bitter flavor but showed a high acceptance rate (&gt;80%).

Ameliorative effects of microencapsulated Spirulina platensis in beverage; physicochemical, simulated release, and organoleptic properties

PurposeThe use of Spirulina sp. in food is limited by its bitter flavour and low absorption in the gastrointestinal system. The purpose of this study is to develop encapsulated Spirulina-alginate beads and to determine the physicochemical properties, the release efficiency in the simulated gastrointestinal fluid and the sensory acceptance of the beads when added into a rose syrup beverage.Design/methodology/approachSpirulina-alginate beads were prepared based on 3 × 3 factorial experiments consisting of three concentrations (1%, 2% and 3%) of plain sodium alginate and three concentrations (1, 3 and 5%) (w/v) of Spirulina. Encapsulated Spirulina-alginate beads were evaluated for their encapsulation effectiveness, size, texture, morphology, colour, in vitro release rate and sensory properties.FindingsSample H (3% sodium alginate + 1% Spirulina) had higher encapsulation efficiency (82.3%) but less protein (38.2 ppm) than Sample J (3% sodium alginate + 5% Spirulina) which produced more protein (126.4 ppm) but had lower encapsulation efficiency (54.5%). Alginate was the primary factor affecting bead size, and the texture became harder at 3% sodium alginate but softer at 5% Spirulina. As the concentration of Spirulina increased, the intensity of the green colour diminished. The encapsulated samples released test was better than the control samples, and Sample B (1% sodium alginate + 1% Spirulina) was preferred by the panellists in the sensory study.Originality/valueThis newly developed encapsulated Spirulina will improve the beverage acceptability, minimize the bitterness and increase the release percentage of Spirulina in simulated gastrointestinal.

Piping hot science: Coffee or tea?

A fine grind Americans drink more than 491 million cups of coffee per day, according to a 2023 report from the National Coffee Association, including the many cups of joe that scientists consume before beginning their daily research grind. One popular coffee-brewing technique is known as espresso, in which water at a near-boiling temperature is pushed through a bed of ground coffee beans at multiple atmospheres of pressure to produce a piping hot drink. Viewed through a scientific lens, the water acts as a solvent in this method to extract the molecules responsible for espresso’s popular bitter flavor. Conventional coffee-brewing wisdom holds that the finer the grind of the coffee beans, the richer the flavor—and science seems to find that line of thinking sensible, as a finer grind provides more surface area for water to dissolve coffee chemicals to make your espresso extra tasty. Not so fast, say leading coffee

CsXDH1 gene promotes caffeine catabolism induced by continuous strong light in tea plant.

Tea plant (Camellia sinensis) is an important cash crop with extensive adaptability in the world. However, complex environmental factors force a large variation of tea quality-related components. Caffeine is essential for the formation of bitter and fresh flavors in tea, and is the main compound of tea that improves human alertness. Continuous strong light stimulation was observed to cause caffeine reduction in tea leaves, but the mechanism is not clear. In this study, the response of tea plant to light intensity was analysed mainly by multi-omics association, antisense oligodeoxynucleotide (asODN) silencing technique, and in vitro enzyme activity assay. The results revealed multiple strategies for light intensity adaptation in tea plant, among which the regulation of chloroplasts, photosynthesis, porphyrin metabolism, and resistance to oxidative stress were prominent. Caffeine catabolism was enhanced in continuous strong light, which may be a light-adapted strategy due to strict regulation by xanthine dehydrogenase (XDH). asODN silencing and enzymatic activity assays confirmed that CsXDH1 is a protein induced by light intensity to catalyze the substrate xanthine. CsXDH1 asODN silencing resulted in significant up-regulation of both caffeine and theobromine in in vitro enzyme activity assay, but not in vivo. CsXDH1 may act as a coordinator in light intensity adaptation, thus disrupting this balance of caffeine catabolism.

Incidencia del tostado sobre las características fisicoquímicas y alcaloides de la cascarilla de cacao (Theobroma cacao) y su efecto en las propiedades organolépticas de una infusión

The objective of this study was to evaluate the incidence of roasting temperature (120, 130 and 140 °C) on the physical-chemical characteristics and husk alkaloids of three cocoa varieties (Forastero, Trinitario and CCN51) and their effect on the organoleptic properties of an infusion. The evaluation of the physical-chemical characteristics (humidity, pHand titratable acidity) was carried out according to INEN standards: 1676:2013; 1842:2013 and 381 respectively and alkaloid analysis (theobromine and caffeine) according to the reference method AOAC 980.14-1998, regarding the organoleptic analysis a 9-point hedonic test was used. This allowed determining that the roasting temperature significantly influences the physical-chemical characteristics and emphasizes that the values of the evaluated parameters decrease as the temperature rises. For alkaloids, it was shown that the type of cocoa husk had an impact on the content. In addition, high roasting temperature reduces theobromine and considerably increases caffeine. For the organoleptic analysis, it was determined that T1 (Forastero + 120 °C) obtained the highest scores in aroma, flavor and acceptability; in relation to color, T4 (Trinitario + 120 °C) stood out with the highest score. Thus, it is attributed that the presence of bitter, acid and astringent flavors in the husk is due to temperatures greater than or equal to 140 °C.

Phenolic compounds, methylxanthines, and preference drivers of dark chocolate made with hybrid cocoa beans

The distinct properties of cocoa beans may be due to their different geographical and genetic origins which lead to chocolates with different characteristics. This study aimed to evaluate dark chocolate samples made with hybrid cocoa cultivated in the Bahia State, Brazil, regarding the composition of bioactive compounds (spectrophotometry, High Performance Liquid Chromatography) and the sensory characteristics (Quantitative Descriptive Analysis-QDA®), and acceptance test). External preference mapping was conducted by first using principal components analysis (PCA) on the QDA data, and then relating consumer preference (overall liking) to this PCA space by regression analysis. Significant differences among the cocoa varieties were found. Chocolate samples that showed lower acceptability also presented higher content of theobromine, caffeine, anthocyanins, and gallic acid which conferred greater intensities of bitterness, astringency, and intense cocoa flavor. Lower levels of methylxanthines, monomeric phenolic compounds, and anthocyanins allowed the highlight of fruity, sweet and caramelized notes, which contributed to greater acceptance of the chocolate samples. Therefore, these characteristics can be recognized as consumer preference drivers (negative and positive, respectively) for the high cocoa chocolates. It seems that higher values of ephicatechin and catechin had no negative contribution to the flavor of the chocolate samples. The findings of this study can be used in agriculture and in the cocoa/chocolate industry, suggesting the potential and applicability of promising hybrid cocoa, with important characteristics, representing a genetic material of great quality, that may be classified as fine flavor cocoa, associating greater value to the product, and contributing to meet consumer needs.

Discovery of the relationship between bitter taste and bitter flavor (efficacy) based on hTAS2Rs

Discovery of the relationship between bitter taste and bitter flavor (efficacy) based on hTAS2Rs

Sensory quality of Citrus scion hybrids with Poncirus trifoliata in their pedigrees.

Hybrids of Poncirus trifoliata L. Raf. with Citrus have shown degrees of tolerance to the deadly citrus greening disease, hence prompting interest as potential commercial varieties. Although P. trifoliata is known to produce fruit that is inedible, fruit from many advanced hybrid trees have not been evaluated for their quality potential. The sensory quality of selected Citrus hybrids with varying degrees of P. trifoliata in their pedigrees is reported herein. Four Citrus×P. trifoliata hybrids developed through the USDA Citrus scion breeding program-1-76-100, 1-77-105, 5-18-24, and 5-18-31-had acceptable eating quality and sweet and sour taste, with mandarin, orange, fruity-noncitrus, and floral flavors. On the other hand, hybrids with higher proportion of P. trifoliata in their pedigrees, US 119 and 6-23-20, produced a juice characterized by green, cooked, bitter, and Poncirus-like flavor and aftertaste. Partial least square regressions revealed that the Poncirus-like off-flavor is likely due to a combination of higher than typical amounts of sesquiterpene hydrocarbons (woody/green odor), monoterpenes (citrus/pine), and terpene esters (floral) and a lack of aldehydes with typical citrus odor (octanal, nonanal, and decanal). Sweetness and sourness were mostly explained by high sugars and acids, respectively. Further, carvones and linalool contributed to sweetness in the samples from early and late seasons, respectively. In addition to highlighting chemical contributors to sensory descriptors in Citrus×P. trifoliata hybrids, this study provides useful information on sensory quality for future citrus breeding efforts. PRACTICAL APPLICATION: The relationships between the sensory quality and secondary metabolites of Citrus×P. trifoliata hybrids described in this study help identify disease-resistant Citrus scion hybrids with acceptable flavor and help mobilize this resistance in future breeding efforts. It also shows potential of such hybrids to be commercialized.

Shoot Nutrition and Flavor Variation in Two Phyllostachys Species: Does the Quality of Edible Bamboo Shoot Diaphragm and Flesh Differ?

For their quality evaluation, it is essential to determine both bamboo shoot nutrition and palatability, which will have a decisive effect on their economic value and market potential. However, differences in shoot nutrition and flavor variation among bamboo species, positions, and components have not been scientifically validated. This study assessed nutritional and flavor differences in two components (i.e., shoot flesh (BSF) and diaphragm (BSD)) of two Phyllostachys species (i.e., Phyllostachys edulis and Phyllostachys violascens) and analyzed any positional variation. Results showed that BSF protein, starch, fat, and vitamin C contents were comparatively higher. Nutrient compounds in the upper shoot segment of Ph. edulis were higher and contained less cellulose and lignin. However, both species' BSD total acid, oxalic acid, and tannin contents were comparable. BSD soluble sugar and sugar:acid ratio were higher than upper BSD total amino acid, four key amino acids (i.e., essential amino acid, bitter amino acid, umami amino acid, and sweet amino acid flavor compounds), and associated ratios were all higher than BSF while also being rich in amino acids. The content and proportion of BSF essential and bitter amino acid flavor compounds in Ph. edulis were high relative to Ph. violascens. Conversely, the content and proportion of BSD umami and sweet amino acid flavor compounds were comparable to that of Ph. edulis. Our results showed that bamboo shoot quality was affected by flavor compound differences and that interspecific and shoot components interact. This study offers a new perspective to determine the formative mechanisms involved in bamboo shoot quality while providing a basis for their different usages.

Three thermal treated methods improve physicochemical and functional properties of wheat bran-germ and the bran-germ containing products.

To fully investigate the effect of different stabilization methods on WBG in the same environment, we studied the effect of microwaving, baking, and extrusion on the nutritional, physicochemical, and processability properties of WBG and whole wheat bran-germ noodle (WBGN). Principal component analysis was used to comprehensively evaluate the qualities of WBG and WBGN. Machine learning-based research was conducted to predict the quality of WBGN based on the features of WBG. The results showed that three methods improved antioxidant ability, bound flavonoids, bound and total phenolics, and the processing properties in WBG (P < 0.05). Extruded-WBG showed a lower polyphenol oxidase activity, lipase activity (35.02 ± 2.02 U and 20.29 ± 0.47 mg g-1 ) and particle size (54.08 ± 0.38 μm), and higher water hold capacity (2.60 ± 0.68%) and bound phenolic levels. The enhanced quantity of bound polyphenols had a major role in the increased antioxidant potential of WBGN. Extruded-WBGN showed higher antioxidant ability for 2,2-diphenyl-1-picrylhydrazyl (171.28 ± 3.16 μmol Trolox eq kg-1 ). The extruded-WBGN had high concentrations of WBG aroma compounds, and low contents of bitterness and raw bran-germ flavor compounds. Next, the enzymatic activity, powder properties, color, and antioxidant capacity of WBG were further utilized to predict the polyphenolic, flavonoids, flavor compounds, and antioxidant capacities of WBGN, where the R2 value of the model exceeded 0.90. The best comprehensive quality modification method of the WBG and WBGN was extrusion, followed by baking and microwaving. The present study shows that extrusion is a promising way to improve WBG into a nutritious and flavorful cereal food ingredient. © 2023 Society of Chemical Industry.

In Vitro Effect of Herbal Medicines with Thermal Characteristics on Heat Sensitivity in Cancer Cells

Background: Cancer remains a major public health threat even though there have been breakthroughs in conventional diagnostics and therapies. Alternatively, treatment with mild hyperthermia and herbal medicine treatment [selected using traditional Korean medicine theory 4 qi (cold, cool, warm and hot) and using the 5 senses of taste (sour, bitter, sweet, pungent and salty)], may be an option to promote cancer cell death in patients where the cancer is accessible.Methods: To investigate effect of combination treatment of herbal medicines and hyperthermia in vitro on cancer cell lines (ACHN, AGS, A549 and U937), the qualities of 38 medicinal herbs and sensitivity to mild hyperthermia (42 and 43°C) treatment were examined. An assay was performed to detect cell viability and proliferation (MTT) following exposure to medicinal herbs and hyperthermia.Results: Heat sensitizing herbal medicines were determined to be larger in the warm and hot groups of medicinal herbs (29.6%) than the groups which were neither warm nor hot (18.2%). In addition, the proportion of heat sensitizing effect with bitter and pungent flavors was 33.3 % and 32.1 %, respectively, greater than the average (26.3 %).Conclusion: In conditions of mild hyperthermia in cancer cell lines, incubation with herbal medicines caused cancer cell death in vitro. These results suggest that the use of traditional herbal medicines with warm, hot, pungent and bitter characteristics may be a useful treatment for cancer using conditions to induce mild hyperthermia and this requires further investigation.