Biostimulation Research Articles

Different biological strategies for the bioremediation of naturally polluted soils

Finding an appropriate method with the highest rate of polycyclic aromatic hydrocarbon (PAH) removal from naturally polluted soils is an important research issue. A pot factorial experiment (using contaminated soil samples from the Isfahan Refinery, Iran) was conducted in a 90-day period to compare the following bioremediation strategies: (1) natural attenuation (NA): the inherent ability of soil for bioremediation; (2) bioaugmentation (BA): inoculating soil with PAH degrading microbes Marinobacter hydrocarbonoclasticus; (3) biostimulation (BS): using N, P and K nutrients for the stimulation of bioremediating soil bacteria to achieve the C : N : P ratio of 100 : 10 : 1, and(4) bioaugmentation + biostimulation (BS + BA). Treatments NA (22.8%) and BA + BS (63.9%) resulted in the least and the highest rate of PAH removal from the soil. The 2–4 ring compounds had a significantly (P ≤ 0.05) higher rate of degradation than the 5–6 ring compounds. The highest rates were resulted by fluorene (76.41%) and acenaphthylene (72.28%) using the BA + BS treatment. However, the lowest degradation rates were resulted by indeno (1,2,3-cd) pyrene (10.05%), benzo [b] fluoranthene (10.17%), benzo (g, h, i) perylene (12.53%), and benzo [k] fluoranthene (13.67%), using NA treatment. The BA + BS treatments are the most effective method for the bioremediation of PAH polluted soils.

LASER IN PERIODONTOLOGY- A REVIEW

Lasers are used in implant and periodontal field practices. Laser has various periodontal applications including calculus removal, decontamination of root and implant surfaces and bio stimulation, incision and ablation, osseous surgery, excision of the soft tissue, and bacterial reduction. There is a strong evidence that laser is used for surgical and nonsurgical periodontal therapies including root bio modification, bacterial decline and decontamination of infected implant surface, and removal of the pocket epithelium.Waterlase® and Periowave™ systems are recent devices that have further revolutionized the laser technology for its favorable clinical applications; however, the procedural cost with the laser device constitutes an obstacle for its routine application.
 Keywords: laser, biomodulation, fluorencence, LANAP

Response Growth and Yield Traits of Faba Bean Genotype to Foliar Feeding with Triplex Amino Biostimulator

A field experiment was carried out in the winter season 2019-2020, in the Nuaimiya region of Fallujah city – Al -Anbar Governorate. The aim of the experiment was to study the response of growth and yield traits of faba bean to foliar feeding with Triplex amino biostimulator. The experiment was applied by using a randomized complete block design (RCBD) according to the arrangement of the split-plot with three replicates. The main plots included three genotypes from the remnants (American, Dutch and Italian), While the sub plots included four concentrations of Triplex amino biostimulator (0, 2.5, 5 and 7.5) g L−1. The Dutch genotype recorded the highest average for each of the number of branches in the plant (8. 84branch.plant−1), leaf area (3289.2 cm2). Plant−1, number of pods per plant (19.25 pods. Plant−1), number of seeds per pod (4.56 seed.pod−1) and seed yield (4.263t.h−1), While the American genotype gave the highest average for plant height (106.70 cm) and 100 seed weight (114.5 g.) The biostimulant significantly effect on all growth and yield traits, the concentration 7.5 g. L−1 gave the highest average for each of Plant height (113.29 cm), number of branches per plant (9.88 branch. plant−1 ), leaf area(3637.9 cm2). Plant−1, number of pods per plant (22.06) pods, number of seeds per pod (4.48 seed.pod−1, weight of 100 seeds (125.2g) and seed yield (4,342 t.h−1). The interaction between the Dutch genotype and concentration 7.5 g. L−1 of the bio stimulant was given the highest average for the number of branches per plant, leaf area, number of pods per plant, and number of seeds per pod.

Wuxal amino (Bio stimulant) improved growth and physiological performance of tomato plants under salinity stress through adaptive mechanisms and antioxidant potential.

In the present study, ameliorative capabilities of wuxal amino (bio stimulant) under salt stress has been investigated through adaptive mechanisms and antioxidant potential in tomato plants. In the experiment, two different concentrations (2 cm L-1 and 3 cm L-1) of wuxal amino through foliar application and soil irrigation were applied to the salt (150 mM) treated tomato plants and then morphological traits, photosynthetic pigments, osmolytes, secondary metabolites, oxidative stress and antioxidant enzymes activity were assessed at 60 days after planting. The results revealed that salt stress decreased the growth parameters, photosynthetic pigments, soluble sugars and soluble protein whereas, content of proline, ascorbic acid, total phenols, malondialdehyde, hydrogen peroxide and the activity of antioxidant enzymes activity increased under salt stress. Moreover, Wuxal amino application through foliar or soil to salt stressed plants improved morphological traits, photosynthetic pigments, osmolytes, total phenol and antioxidant enzymes activity. Interestingly, the deleterious impact of salinity on tomato plants were significantly reduced and it can be evident from reduced MDA and H2O2 levels. These responses varied with the mode (foliar or soil) of application of Wuxal amino under different concentrations (2 cm L-1 and 3 cm L-1). It was concluded that application of Wuxal amino (2 cm L-1, foliar) and (3 cm L-1; soil) proved best and could be commercially used as eco-friendly tool for the protection of tomato plants grown under salinity stress.

THE EFFICIENCY OF ORGANIC BIOSTIMULANTS FOR KOHLRABI SEEDS AND PLANTS TREATMENT

The research reveals a versatile positive effect of organic bio stimulants Regoplant, Stimpo, and Emochka on kohlrabi cabbage plants. Soaking the seeds for 12 hours and foliar dressing of plants during vegetation with solutions of growth regulators contributed not only to increase the total yield of stems but also to enhancing growth indices of kohlrabi plants and at the same time ensuring better resistance to adverse growing conditions. The highest yield was obtained for the soaking seeds for 12 hours and 2 foliar dressings. Thus, in kohlrabi varieties ‘Delikatesna Bila’ and ‘Violeta’, the highest yield of stems (23.9?25.2 t/ha) was obtained under the influence of growth regulators Regoplant and Stimpo. In the experiment, a high yield of commodity stems was obtained in variety ‘Delikatesna Bila’ (86?93%) and in variety ‘Violeta’ (86?91%), which was on average higher by 8.0 and 5.6% than in the control treatment. The use of biostimulants ensured the yield of soluble dry matter higher by 0.2%, 0.4%, 0.8%in‘Delikatesna Bila’ and by 0.8%, 1.0%, 0.3% in ‘Violeta’, respectively, compared to the control treatment

The Effect of Spraying the Bio stimulant humic acid on growth and yield of Four sunflower hybrids

A field experiment was carried out in a salty loam at Mysiab which is located 3 km from Mysiab Technical college during the spring season of 2016/2017. The aim of the experiment was to study the influence of spraying the bio stimulant humic acid with the following levels (0, 2ml, 4ml and 6ml.litre−1), on growth and yield of four sunflower hybrids (Zuhraa, Qudas, Euflor and Cobon). Results showed that, excluding plant dry weight, Capitulum diameter and kernel weight where there were no significant differences between the spring levels 4ml and 6ml.litre−1. Take spring level 6ml.litre−1 significantly overcome the other levels on the remainder trails being studied. Excluding plant height, where there was no significant effect between the hybrids Zuhraa and Qudas; Zuhraa was superior with remainder characters among other hybrids. The interaction effect was significant with the over whole studied characters. The seed yield was 6.32 tone.hectare−1 for Zuhraa and Euflor. Thus we can introduce those two hybrids in breeding projects to achieve rales which could tolerate the region environmental stresses.

Main Approaches to the Use of Biomaterials in Oral and Maxillofacial Surgery: A Brief Systematic Review


 
 
 
 Introduction: In recent years, procedures with the use of dental implants have increased worldwide, reaching approximately one million dental implants per year. In recent years, a platelet concentrate called FRP (fibrin-rich plasma) has been the subject of clinical studies. Associated with this, the biomaterial Bio-Oss® (Geistlich), as it is biodegradable, biocompatible, non-toxic, and has low immunogenicity, and bio stimulators can act in the regeneration of bone tissue, as it establishes with the cells the appropriate biological niche (favorable microenvironment) for bone growth. Objective: Therefore, the present study aimed to evaluate, through a brief systematic review, the results that involve bone formation for dental implantation, with the use of biomaterials such as fibrin-rich plasma and Bio-Oss®. Methods: The model used for the review was PRISMA. Was used databases such as Scopus, Scielo, Lilacs, Google Scholar, PubMed. Results: Fibrin-rich plasma (FRP) as an autologous biomaterial for use in oral and maxillofacial surgery presents most leukocytes, platelets, and growth factors, forming a fibrin matrix, with three-dimensional architecture. The Bio-Oss® biomaterial (Geistlich), as it is biodegradable, biocompatible, non-toxic, and has low immunogenicity and bio stimulators can act in the regeneration of bone tissue, since it establishes with the adenomatous mesenchymal stem cells the appropriate biological niche for bone growth and, thus, allowing the dental implant to be as effective as possible. Conclusion: The use of FRP associated with Bio-Oss® seems to illustrate high success rates with minimal costs, which may reduce the amount of bone graft needed to fill the sinus cavity, reducing the costs of the procedure.
 
 
 

Incidencia del sombreado, biorreguladores y bioestimulante en el desarrollo y rendimiento del pasto Mombaza (Panicum maximum Jacq.)

Shading (S), bioregulators (BR) and biostimulants (BS) techniques are alternatives that promote sprouting and production of grass. The objective was to evaluate the effect of S, BR and BSt on the growth and yield of Mombaza grass in Ecuador. A complete randomized block design, with split plot arrangement and three replications, was used, the main plot represented by condition S (S1: full solar exposure, S2: shade of trees) and secondary one by BR and BS application (A0: control; A1: minimum doses of BR, 250 mL.ha-1 Cytokin + 10 g.ha-1 New Gibb 10 %; A2: maximum doses of BR, 500 mL.ha-1 Cytokin + 20 g.ha-1 New Gibb 10 %; A3: commercial dose of BS Algamar, seaweed, 750 g.ha-1). Three cuts of grass were made, 35 days after staring experiment; in each one, tiller height (TH), number of tillersm-2 (NTM) and stemstiller-1 (NST), and dry matter yield (DMY) were evaluated. It is found effects (P<0.05) of S on the four variables in each cut; and application of BR and BS on TH in cuts 1, period of least precipitation. It is concluded that the grass cultivated in S1 increased the growth and DMY in cuts 1, 2 and 3 (0.180, 0.300 y 0.398 kg.m-2). NTM was stimulated with S2 and TH with the minimum dose of BR.

A Synergistic Approach of Injectable-Platelet Rich Fibrin and Bio Stimulation in the Treatment of Oral Lichen Planus

A Synergistic Approach of Injectable-Platelet Rich Fibrin and Bio Stimulation in the Treatment of Oral Lichen Planus

Identification of Beneficial Microbial Consortia and Bioactive Compounds with Potential as Plant Biostimulants for a Sustainable Agriculture.

A growing body of evidence demonstrates the potential of various microbes to enhance plant productivity in cropping systems although their successful field application may be impaired by several biotic and abiotic constraints. In the present work, we aimed at developing multifunctional synthetic microbial consortia to be used in combination with suitable bioactive compounds for improving crop yield and quality. Plant growth-promoting microorganisms (PGPMs) with different functional attributes were identified by a bottom-up approach. A comprehensive literature survey on PGPMs associated with maize, wheat, potato and tomato, and on commercial formulations, was conducted by examining peer-reviewed scientific publications and results from relevant European projects. Metagenome fragment recruitments on genomes of potential PGPMs represented in databases were also performed to help identify plant growth-promoting (PGP) strains. Following evidence of their ability to coexist, isolated PGPMs were synthetically assembled into three different microbial consortia. Additionally, the effects of bioactive compounds on the growth of individually PGPMs were tested in starvation conditions. The different combination products based on microbial and non-microbial biostimulants (BS) appear worth considering for greenhouse and open field trials to select those potentially adoptable in sustainable agriculture.

The Effect of Fertigation and Foliar Application of Seaweed’s Bio Stimulant on Banana Yield

In order to shed light on the effect of the seaweed Ascophyllum nodosum (L.) bio stimulant on the banana fruit, foliar application (Fo), fertigation(F1) and a mix of both interventions (M) were done during 2018-2019 season on banana plants grown in South Lebanon compared with control (Ctrl). The period between flowering and harvest, the bunch weight, the weight and dimensions of fruit, were measured during the study. The results showed that the shortest period between flowering and harvest was observed on samples treated through foliar application (Fo), less with fertigation and then the mix of both (M). Those treatments were very effective in stimulating the weight of bunch and length of fruit, while a significant increase in the weight of fruit was noticed when both applications were done together (M). All treated modalities showed better performance compared with control. There was no difference in the diameter of fruit between all treatments. Therefore, the application of eco-friendly seaweed-based bio stimulant would be beneficial for the time of harvesting, and the size of banana fruits.

Influence of bulb treatment with Bio-fertilizers and foliar spray of Bio-stimulants on growth and flowering of tuberose (Polianthes tuberosa L.) cv. Suvasini

This study was conducted to assess the Influence of Bulb treatment with Bio-Fertilizers and foliar spray of Bio-Stimulants on growth and flowering of Tuberose (Polianthes tuberosa L.) cv. Suvasini. The experiment was laid out in Contrast Factorial Randomised Blocked Design consisting of two factors with three and eight levels respectively. Replicated thrice at SKLTSHU, college of horticulture, Mojerla. Tuberose bulbs where treated with three Bio-Fertilizers viz., phosphate solubilizing bacteria (PSB), Azospirillum (AZO) and Potassium solubilizing bacteria (KSB) each 200 g/l and they are foliar sprayed by eight Bio-Stimulants gibberellic acid (GA3), salicylic acid (SA), cycocel (CCC) and humic acid (HA) each at 200 ppm and 400 ppm. Among bio fertilizers PSB (Phosphate solubilizing bacteria) and among bio stimulants GA3 400 ppm. while in interaction effect PSB (Phosphate solubilizing bacteria) 200 g/l along with GA3 (Gibberellic acid) 400 ppm has resulted significantly best in Plant height (cm), Number of leaves per plant, Length of leaf (cm), Days taken to spike emergence, Spike length (cm), Diameter of spike (cm), Rachis length (cm), Number of florets per spike, Number of spikes per plant, Flower longevity (days), Flower yield or spike yield per hectare (in lakhs), when compared with other treatment combinations and control.

Comparative evaluation of wastewater and bioventing system for the treatment of acid mine drainage contaminated soils

The increasing rate of soil pollution poses a prolific threat to the sustenance of the ecosystem. Acid mine drainage (AMD) is an environmental problem that has contributed immensely to soil pedogenesis and contamination associated with mining activities. Physical, chemical, and thermal methods of soil treatment have some rooms for improvement to meet remediation purposes. Contrary to other methods, bioremediation is a cost-effective and a sustainable biological approach that utilizes microorganisms for the remediation of a contaminated environment and does not recontaminate or further degrade the soil after application. This study aims to comparatively evaluate the application of wastewater and bioventing system for the the treatment of AMD contaminated soils. A microcosm containing 1 kg of contaminated soil was amended with wastewaters of varying (biostimulation (BSTa)) while bioventing treatment (BVTa) consists of the injection of atmospheric air through the vadose zone into bioreactors and amended with wastewater effluents. The treatment was conducted at ambient condition for 28 days. The result of the treatment recorded 28–67% and 58–70% metal (Mn, Fe, Al, Cu and Zn) removal efficiencies with 51% and 34% sulfate removal efficiencies for BSTa and BVTa treatments, respectively. However, attempts to improve the sulfate removal efficiencies through the injection of atmospheric air were not effective. This study showed that wastewater and bioventing can be effectively applied as a potential alternative for the treatment of AMD contaminated soils.

Bioremediation of toluene by bioaugmentation, biostimulation and natural attenuation

Contamination in subsurface environment is a serious environmental hazard. Main sources of the contamination are petrol, diesel fuel, gasoline at oil refineries, underground storage tanks, transmission pipelines and different industries. Permeable reactive barriers (PRBs), which is a promising technology to remediate groundwater in-situ, are filled with reactive materials for the removal of the contaminants present in groundwater. In this study, groundwater contaminated with toluene is treated in reactor columns by biological processes. This study was conducted to assess the impact of bioaugmentation (BA) and biostimulation (BS) on toluene degradation efficiency. After 44 days of treatment, toluene concentrations were decreased from 5 mg/l to 4.304 mg/l by the natural attenuation treatment (Reactor 2), which represents a 13.9% removal efficiency. Toluene was reduced to 0.0239 mg/l in the biostimulation and bioaugmentation treatment (Reactor 1), which represents a toluene removal efficiency of 99.5%. This study showed that the toluene removal efficiency in the combined BA and BS process was much higher than in natural attenuation (NA) process tested.

Impact of some agricultural technology intervention in moutala village of west bengal towards doubling farmers’ income

Agriculture sector plays a pivotal role for transformation of rural economy in India. Most of the time, farming community fails to identify and implement suitable agro-technology for sustainable production. In this backdrop, attempts have been made by Sasya Shyamala KVK to develop a model village using modern and relevant agricultural technologies that could enhance production as well as farm income. Based upon the survey, Moutala village under Baruipur block of West Bengal was selected for technology show-casing suitable for their dissemination. After analysing different PRA tools, it has been observed that chilli cultivation, winter vegetables cultivation, backyard poultry rearing and homestead piggery were prime areas for technological intervention with a view to increase family income. Therefore, training and frontline demonstration of cutting edge technologies were conducted during the year 2016–17. Implemented technologies included: border netting and organic bio stimulant in chilli leaf curl management, cultivation of high value winter vegetables, development of micro hatchery for improvement of IFS based poultry farming and existing pig breed replacement using Ghungroo piglets. After successful demonstration, it was found that significant amount of additional income was incurred after adopting above technologies. It can be concluded that these proven technologies have the potential for doubling farmers’ income and should be integrated in IFS models.

The effectiveness of bio stimulants on irrigated meadow soils in the cultivation of corn

The effectiveness of bio stimulants on irrigated meadow soils in the cultivation of corn

Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons

Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera Pseudomonas, Rhodococcus and Acinetobacter. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA/P) and bioaugmentation with inoculum pre-grown in acetate (BA/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA/P and BA/A but not in NA. Acetate was the best substrate for consortium growth. BA/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills.

Pure Organic Active Compounds Against Abiotic Stress: A Biostimulant Overview.

Biostimulants (BSs) are probably one of the most promising alternatives nowadays to cope with yield losses caused by plant stress, which are intensified by climate change. Biostimulants comprise many different compounds with positive effects on plants, excluding pesticides and chemical fertilisers. Usually mixtures such as lixiviates from proteins or algal extracts have been used, but currently companies are interested in more specific compounds that are capable of increasing tolerance against abiotic stress. Individual application of a pure active compound offers researchers the opportunity to better standarise formulations, learn more about the plant defence process itself and assist the agrochemical industry in the development of new products. This review attempts to summarise the state of the art regarding various families of organic compounds and their mode/mechanism of action as BSs, and how they can help maximise agricultural yields under stress conditions aggravated by climate change.

Grapevine Stimulation: A Multidisciplinary Approach to Investigate the Effects of Biostimulants and a Plant Defense Stimulator.

The increasing use of plant defense stimulators (PDS) and biostimulants (BS) to make agriculture more sustainable has led to questions about their action on plants. A new PhysBioGen approach is proposed with complementary tools: PHYSiological (root weight); BIOchemical and BIOlogical (secondary metabolite quantification and Plasmopara viticola development) and expressions of 161 GENes involved in metabolic plant functions. The proposed approach investigated the effects of three phytostimulants on Vitis vinifera: one PDS (ASM) and one BS chelated (CH) and another enriched with seaweed (SW). Distinct responses were obtained between the PDS and the two BS. In particular, we observed the persistence of anti-mildew efficacy over time, correlated with differentiated expressions of defense genes (VvROMT, VvSAMT, VvPR8). As expected, the two BS displayed more similarities to each other than to the PDS (flavonols, anthocyanins, free salicylic acid). However, the two BS revealed differences in the modulation of genes involved in defense and primary metabolism and some genes were identified as potential markers of their action (VvWRKY1, VvLOX9, VvPOD, VvPDV1, VvXIP1, VVDnaJ). Our results highlight the common and the specific effects of the two BS and the PDS. These new tools could help in understanding the mode of action of phytostimulants in order to achieve better quality and production yield and/or as a way to limit chemical inputs in the vineyard.

Bioremediation perspective of historically contaminated sediment with polycyclic aromatic hydrocarbons

The purpose of the current study is to compare the influence of different aerobic conditions (biostimulation (BS), bioaugmentation (BA), and a combination of biostimulation and bioaugmentation (BB)) on polycyclic aromatic hydrocarbons (PAH) degradation and compare the degraded amount with single step XAD-4 extraction as a new tool for bioavailability assessment for chronically contaminated sediment samples obtained from territory of Autonomous Province Vojvodina of Serbia (S1, S2, and S3). A great number of papers dealing with biodegradation of PAHs in spiked sediment or soil have been published, but to the authors’ knowledge, a limited number of papers studied aged, historically polluted sediment and a sum of chosen U.S. Environmental Protection Agency (USEPA) PAHs. A significant reduction (up to 67%) in PAH concentration was observed, while the percentage of reduction varied depending on the sediment sample and treatment used. BS treatment successfully stimulated growth of indigenous bacteria. Further, PAH-degrading strain Sphingomonas paucimobilis F8 inoculated in BA and BB treatment survived for up to 7 weeks after it was suppressed by unfavorable conditions or native microbes. Degraded amounts generally showed good correlation with results obtained from XAD-4 extraction. Results obtained in the current study represent a good start for standardizing a XAD-4 extraction technique as a simplified, easier, and lower cost method for bioavailability assessment.