Development Of Bioproducts Research Articles

English

Adequate formulation of bioproducts represents one of the most challenging aspects of bioproduct development. The incorporation of adjuvants with bioagents can positively influence product development. However, it is indispensable to evaluate the sensitivity of bioagents to these adjuvants. The aim of this study was to determine the toxicity of seven adjuvants at different concentrations to Bipolaris yamadae (Bipolaris euphorbiae) fungus, to select a product compatible with this phytopathogen for a wettable powder formulation and to evaluate the pathogenicity of the formulation against wild poinsettia (Euphorbia heterophylla). The powder fraction of the formulation was made up of 1% anti-wetting silicon dioxide mixed with B. yamadae conidia to a final concentration of 107 conidia.ml-1. The aqueous fraction was composed of 0.1% Geropon T36 compatibilizer, 0.075% silicone, the dispersant, 0.1% Tween 80 or tensioactive and 0.5% PVP K30 or spreading agent. The incidence of disease was observed in 83.6% of the plants inoculated with the formulated fungus, which was 79.0% higher than that in the plants inoculated with the bioagent only. These findings strongly suggest that the new formulation successfully controls Euphorbia heterophylla and greatly increases the pathogenicity of the fungus.   Key words: Bipolaris yamadae, Euphorbia heterophylla, adjuvants, biological control, phytopathogenic fungi.

Comparative study of the chemical profile of the parotoid gland secretions from Rhaebo guttatus from different regions of the Brazilian Amazon

Amphibian cutaneous secretion has great potential for bioprospection and is a great tool in the development of bioproducts. Thus, the objective of the present work was to evaluate the comparative study of the chemical profile parotoid gland secretions from Rhaebo guttatus collected in two distinct regions of the Brazilian Amazon. For this, the chemical composition of six methanolic extracts of this species were analyzed by Liquid Chromatography in UV and MS Detection Ultra-Chromatography Systems (UFLC-DAD-micrOTOF). All obtained chromatograms presented two distinct regions; one referring to the more hydrophilic molecules (alkaloids), while the other refers to the more hydrophobic compounds (steroids). The steroid region resembles all samples, regardless of where they were collected. In the alkaloid region, there was a standardized variation for the samples from the southern Brazilian Amazon, but the same was not true for the samples collected in the Amazon-Cerrado transition region. Thus, the data suggest that the environment and diet of R. guttatus may be important in alkaloid production, but do not influence steroid content. These results add new information about the poison of the toad R. guttatus and raises new questions to be further investigated, thus contributing to the knowledge of the anuran fauna of the Brazilian Amazon.

Cola caricifolia (G.Don) K. Schum and Crotalaria retusa L. from Ivory Coast as sources of bioactive constituents

Cola caricifolia (G.Don) K. Schum and Crotalaria retusa L. are important plants in African traditional medicine used against a plethora of human ailments. In this study, phytochemical characterization and enzyme inhibitory assays were performed to assess the potential usefulness of C. caricifolia and C. retusa leaves extracts as source of bioactive constituents for potential industrial applications. The flavonoid content of C. caricifolia (22.27 mg rutin equivalent (RE)/g) was significantly higher (p<0.05) than C. retusa (12.45 mg RE/g) methanol extract. Inhibitory activity against acetylcholinesterase, butyrylcholinesterase, and tyrosinase were evaluated for all the extracts (ethyl acetate, methanol, and water), whereby the best activity recorded for the methanol extracts. Additionally, the methanol extracts of C. caricifolia and C. retusa showed significant antioxidant activity in the phosphomolybdenum, metal chelating, reducing power, and radical scavenging assays. Flavonoid glycosides and caffeoyl derivatives were the most abundant constituents in C. caricifolia. Crotalaria retusa contained several flavonoid glycosides with structures not described previously. One new compound, kaempferol-6-C-β-glucopyranoside-3-O-β-glucopyranosil-6-O-caffeoyl ester has been elucidated using spectroscopic method in the current study. Derivatives present in minor amount were tentatively identified as kaempferol glycosides. Overall phytochemical investigations allowed the identification of flavonoid O and C glycosides, caffeic acid derivatives, and phytosterols from both plant extracts while pyrrolizidine alkaloids were identified in C. retusa. Structural characteristics of the new isolated compounds along with the most abundant constituents of the two extracts were elucidated allowing the observation of some structural moieties related to the observed activity. These observations highlighted the potential use of these plants as a source of multi-directional biological agents that could lead to bioproducts development.

Modern and traditional extraction techniques affect chemical composition and bioactivity of Tanacetum parthenium (L.) Sch.Bip

Tanacetum parthenium (L.) Sch.Bip (TP), a flowering plant, is famous in traditional medicine to prevent migraine and headache. However, there is currently a dearth of studies to advocate the phytochemical profile and biological propensities of extracts prepared from this plant. This study endeavors to highlight the biological potential of TP extracts prepared by modern (ultrasound-UAE, microwave-MAE and accelerated-ASE extractions) and traditional (maceration and Soxhlet) extraction techniques. The chemical profile of the extracts was established via ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique. Sixty different polyphenolic compounds belonging to the classes of phenolic acids, flavonoid glycosides and flavonoid aglycones were recorded in the extracts. Additionally, the quantity of 17 components was measured using appropriate standards and it was found that the modern extraction gave extracts with the higher content of observed compounds than the traditional techniques. Evaluation of antioxidant activity was determined in vitro via five standard assays. The inhibitory potential of TP extracts against key enzymes implicated in the non-communicable diseases such as diabetes (α-amylase and α-glucosidase), neurodegenerative diseases (acetyl- and butyrylcholinesterase) and skin diseases (tyrosinase), was assessed. Potent antioxidant ability of all TP extract was revealed with a predominance for the extracts yielded using the ASE method. This potent antioxidant activity of the extracts corroborated with the high phenolic (65.05 ± 0.27 mg gallic acid equivalent (GAE)/g extract) and flavonoid contents (55.40 ± 0.52 mg rutin equivalent (RE)/g extract). Tanacetum parthenium extracts also showed significant α-glucosidase inhibitory activity (1.63–1.67 mmol acarbose equivalent (ACAE)/g extract) and moderate inhibition activity against α-amylase (0.51-0.56 mmol ACAE/g extract). The extracts also showed potent activity against cholinesterases and tyrosinase. This study tend to validate the use of TP extracts obtained by novel extraction techniques such as ASE, as potent bioactive extracts to be further studied for therapeutic bio-product development.

The Integral Role of Bioproducts in the Growing Bioeconomy

Bioproducts refer to industrial chemicals and materials other than fuels that are refined from renewable biological resources, or any domestic consumables manufactured with such chemicals and materials. Bioproducts development is as essential a pathway for valorizing biomass materials as biofuels. Integrated production of biofuels and bioproducts has been identified as a key strategy to improve the economics of biorefineries and the sustainability of the bioeconomy. This paper reviews the roadmap, status, and major challenges of bioproducts development. Currently there are more than 15,000 marketable bioproducts in five broad classes: biochemicals, biopolymers, bioadhesives, biomedicines, and biopesticides. These bioproducts are manufactured from diverse biomass feedstocks using biorefining technologies similar to those for biofuels. Food crops, specialty plants, lignocellulosic residues, and algal biomass can be pretreated with efficient separation techniques to extract starch, sugars, (hemi)cellulose, proteins, lipids, fibers, and specialty chemicals and further processed into valuable bioproducts through fermentation or chemical transformation. Lignocellulosic biomass can be hydrolyzed to simple sugars for manufacturing biochemicals and bioplastics via fermentation and polymerization. It can also be converted to bio-oil and syngas using pyrolysis and gasification techniques and subsequently to valuable chemicals through Fischer-Tropsch synthesis. Biowaste materials can be processed via anaerobic digestion to produce biogas, a primary industrial feedstock for numerous chemicals and plastics. The most pressing challenge of bioproducts development is to improve the economics of biorefineries through biotechnology innovations and cross-sector integration. Effective integration of biofuel and bioproducts production requires technological and financial support and life cycle assessment-based planning. The global bioproducts market value was estimated at US$282 billion in 2016 and forecast to expand annually by 11% in the next five years.

Preface

Research Centre for Biotechnology, Indonesian Institute of Sciences are pleased and proud to inform you, the 6th-International Symposium on Innovative Bio-production (ISIBio2019), which was held on October 23-24th, 2019 at ICE BSD City, Tangerang, Indonesia. We have received high appreciation from 102 attendants which were come from science, education, and industries communities.The theme for ISIBio2019 is “Enhancing Bioproducts Development by Biotechnological Approach”. We hope this seminar can accelerate the innovative bioproduction in many sectors such as energy, food, agriculture, and health in Indonesia. The seminar also provide 6 experts from 3 countries (Indonesia, Japan, and United Kingdom) to share their knowledge and expertise related to technological advances and research result in bioproduction fields. We also have received more than 70 fullpapers (both for poster and oral presentation) from various institution and university in Indonesia and Japan.This international seminar will be held continuously with full of honour will dedicate this proceeding as a contribution to biotechnology and bioengineering fields in Indonesia specially and to support the international cooperation with others renowned scientists, professors, and research professionals, young researchers, industrial delegates and student communities across the globe under a single roof, where they can discuss research, achievements, and advancements in biotechnology and bioengineering fields for the bright future.Finally, the opportunities of the continuously symposium will present us to be able to continue fulfilling our great intentions in biotechnology field for better future in science and technology. Dr. Ade Andriani Chairman of ISIBio 2019

Engineered Microbes for Pigment Production Using Waste Biomass.

Agri-food waste biomass is the most abundant organic waste and has high valorisation potential for sustainable bioproducts development. These wastes are not only recyclable in nature but are also rich sources of bioactive carbohydrates, peptides, pigments, polyphenols, vitamins, natural antioxidants, etc. Bioconversion of agri-food waste to value-added products is very important towards zero waste and circular economy concepts. To reduce the environmental burden, food researchers are seeking strategies to utilize this waste for microbial pigments production and further biotechnological exploitation in functional foods or value-added products. Microbes are valuable sources for a range of bioactive molecules, including microbial pigments production through fermentation and/or utilisation of waste. Here, we have reviewed some of the recent advancements made in important bioengineering technologies to develop engineered microbial systems for enhanced pigments production using agri-food wastes biomass/by-products as substrates in a sustainable way.

Recent Advances in Chain Conformation and Bioactivities of Triple-Helix Polysaccharides.

Natural polysaccharides derived from renewable biomass sources are regarded as environmentally friendly and sustainable polymers. As the third most abundant biomacromolecule in nature, after proteins and nucleic acids, polysaccharides are also closely related with many different life activities. In particular, β-glucans are one of the most widely reported bioactive polysaccharides and are usually considered as biological response modifiers. Among them, β-glucans with triple-helix conformation have been the hottest and most well-researched polysaccharides at present, especially lentinan and schizophyllan, which are clinically used as cancer therapies in some Asian countries. Thus, creation of these active triple-helix polysaccharides is beneficial to the research and development of sustainable "green" biopolymers in the fields of food and life sciences. Therefore, full fundamental research of triple-helix polysaccharides is essential to discover more applications for polysaccharides. In this Review, the recent research progress of chain conformations, bioactivities, and structure-function relationships of triple-helix β-glucans is summarized. The main contents include the characterization methods of the macromolecular conformation, proof of triple helices, bioactivities, and structure-function relationships. We believe that the governments, enterprises, universities, and institutes dealing with the survival and health of human beings can expect the development of natural bioproducts in the future. Hence, a deep understanding of β-glucans with triple-helix chain conformation is necessary for application of natural medicines and biologics for a sustainable world.

Bio-products market in Georgia: current challenges and development perspectives

The article explores the organic food market in the world and in Georgia. The growth tendency of demand for bio-products is assessed. For Georgia, bio-production is considered as an alternative to the traditional system of agricultural production. The paper examines the experience of European countries. The ways for development of production of bio-products in Georgia are identified by comparative analysis; opportunities for population employment and income growth, nutrition quality, and environmental improvement are substantiated through the development of bio-production. The authors have studied the state programs for production of bio-products and sales promotion; conclusions were made on the feasibility of implementing adapted programs in Georgia. Qualitative research was carried out in order to identify the problems and directions of development in the field of bio-production and bio-market development; through the impact and urgency matrix were identified the barriers to bio-production, drawn conclusions on the identified problems and proposed appropriate recommendations.

Bio-products market in Georgia: current challenges and development perspectives

The article explores the organic food market in the world and in Georgia. The growth tendency of demand for bio-products is assessed. For Georgia, bio-production is considered as an alternative to the traditional system of agricultural production. The paper examines the experience of European countries. The ways for development of production of bio-products in Georgia are identified by comparative analysis; opportunities for population employment and income growth, nutrition quality, and environmental improvement are substantiated through the development of bio-production. The authors have studied the state programs for production of bio-products and sales promotion; conclusions were made on the feasibility of implementing adapted programs in Georgia. Qualitative research was carried out in order to identify the problems and directions of development in the field of bio-production and bio-market development; through the impact and urgency matrix were identified the barriers to bio-production, drawn conclusions on the identified problems and proposed appropriate recommendations.

Bioherbicidal action of Phoma dimorpha fermented broth on seeds and plants of Senna obtusifolia 1

ABSTRACT The weed Senna obtusifolia causes severe damages to pasture areas in Brazil, due to its high production and seed spread. This study aimed to evaluate the bioherbicidal action of Phoma dimorpha fermented broth in the pre-emergence and post-emergence of S. obtusifolia. The experimental design was completely randomized, with two treatments, one with and other without the application of the fermented broth. The bioherbicidal effects were measured in bioassays of pre-emergence (germination percentage), detached leaves (phytotoxicity) and post-emergence (phytotoxicity, plant height and fresh plant mass). The application of the fermented broth provided a pre-emergence bioherbicidal action, inhibiting the seed germination in 100 %. In detached leaves, it caused leaf necrosis and death on the ninth day after the application. In the post-emergence, this application caused moderate symptoms, such as leaf spots and reduction in the weed plant size. It was concluded that the P. dimorpha fermented broth has a potential herbicidal action and, therefore, represents an alternative in the development of bioproducts for a sustainable weed control in pastures.

Selection and molecular characterization of Bacillus thuringiensis strains efficient against soybean looper (Chrysodeixis includens) and Spodoptera species

ABSTRACT Soybean looper (Chrysodeixis includens) and Spodoptera group are important defoliators insects responsible for significant yield losses in soybean, cotton and maize in Brazil. Bioinsecticides and transgenic plants expressing insecticidal proteins from Bacillus thuringiensis are among the most used sustainable control methods for pest control in agriculture, especially for several lepidopteran species. To provide new components for insect control, this study aimed to select and characterize B. thuringiensis strains toxic to C. includens, S. cosmioides, S. eridania, and S. frugiperda. We performed initial bioassays with fifty B. thuringiensis strains for C. includens and selected four strains – 1608A, 726, 773, and 775E – that caused high larval mortality (100%) to be tested against Spodoptera species. These strains harbored cry insecticidal genes, megaplasmids, absence of β-exotoxin and showed two major proteins about 65 and 130 kDa in SDS-page attributed to Cry protein classes toxic to lepidopteran species. The 1608A and 775E strains also showed high toxicity to Spodoptera species that demonstrate great potential for B. thuringiensis-based bioproduct development and as sources of new insecticidal genes for transgenic crops for multiple pest control relevant in the Brazilian agricultural system.

Impact of different extraction solvents and techniques on the biological activities of Cirsium yildizianum (Asteraceae: Cynareae)

Several Cirsium species have been used in folk medicine for the management of human ailments. However, there is a paucity of scientific data regarding their biological activity as in the case of the new species, C. yildizianum, from Anatolia, Turkey. The present study endeavours for the first time to appraise the antioxidant and enzyme inhibitory activity of C. yildizianum. The phytochemical profiles of C. yildizianum extracts obtained using homogeniser-assisted extraction (HAE), ultrasound-assisted extraction (UAE), Soxhlet extraction, maceration, decoction, and infusion, using methanol or water as extraction solvents, was determined by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC–ESI-MSn) analysis. In all extracts, flavonoids were the most abundant compounds, particularly luteolin and apigenin glycosides. HAE methanol extract presented the highest phenolic (37.10 mg gallic acid equivalent/g) and flavonoid (46.78 mg rutin equivalent/g) contents. Likewise, HAE-methanol extract showed potent radical scavenging (40.76 and 68.13 mg Trolox equivalent [TE]/g, for DPPH and ABTS, respectively) and reducing properties (127.62 and 89.95 mg TE/g, for CUPRAC and FRAP, respectively). HAE-methanol extract showed inhibitory activity against acetylcholinesterase (AChE) (3.57 mg galantamine equivalent [GALAE]/g). UAE-methanol extract was a potent inhibitor of butrylcholinesterase (BChE) (2.72 mg GALAE/g) and tyrosinase (121.06 mg kojic acid equivalent/g) However, poor inhibition was recorded for enzymes targeted in the management of diabetes type II, namely α-amylase and α-glucosidase. On the other hand, potent metal chelating property was observed for water extracts. This study provides comprehensive scientific information on the phytochemical profile of C. yildizianum extracted using different procedures and extraction solvents, which might be considered as valuable baseline data for future bioproducts development.

A fuzzy analytic hierarchy process for the site selection of the Philippine algal industry

The Philippine algae industry is a multi-billion dollar industry that requires restructuring to further gain global market share. Through the development of new algal bioproducts by strategically repositioning and enabling collaboration with existing industries, the Philippine algal industry aims to enhance its economic status. The decentralized locations of regions in the Philippines make it challenging to select a potential site for the industry. A decision support system is proposed to aid the industry for site selection that evaluates the different regions based on environmental impact, costs, social aspects, and industry presence. In addition, the site assessment considers the viewpoints of the various stakeholders to arrive at a sound and just decision. Thus, a fuzzy analytic hierarchy process (FAHP) method is employed to include the uncertainty in the subjectivity in the viewpoints of different stakeholders such as the academe, the government, and the industry. In addition, the FAHP approach is capable of combining both qualitative and quantitative data. The combined results revealed the level of importance of the main criteria with a combined weight of 43% for the environmental impact, 22% for the costs, 21% for the social aspects, and 14% for the industry presence. The disparity of priorities was observed among the stakeholders where the industry chose costs at 29% above other criteria when compared to the government and the academe which chose environmental impact at 44% and 40%, respectively, among other criteria. The highly preferred sites for the Philippine algae industry were Calabarzon, Northern Mindanao, Western Visayas, and Central Luzon due to the good potential labor force, presence of industries, and available resources in the regions. In order to achieve a harmonious prioritization of criteria among the stakeholders, policies on the encouragement of public investment on regions with marginal income must be considered.

Developing freeze-dried bioproducts for the Russian military in the Arctic

The Arctic is currently a strategic region of Russia and it requires military protection. Good nutrition is a prerequisite for successful performance of the servicemen in such extreme conditions. The Russian armed forces do not have a special ration for the Arctic region. The existing rations lack products enriched with biologically ac- tive substances, probiotics, prebiotics, or vitamin and mineral complexes. Subcaloric nutrition, even with short-term physical exertion, in combination with low temperatures deprives the human body of vitamins and minerals. There- fore, the development of freeze-dried bioproducts with functional properties is highly relevant. This article presents the results of a study aimed to develop new freeze-dried milk-based bioproducts for the Russian military in the Arc- tic. For this, we created a microbial consortium of lactic acid bacteria with a wide spectrum of antimicrobial activity. We also used a range of functional ingredients, namely pectin, pine nut meal, a ‘Lactobel’ prebiotic protein-and-car- bohydrate product, rowan puree, and rosehip syrup in the amounts established during the study. The new freeze- dried milk-based bioproducts named ‘Pobeda’, enriched with combiotics and metabiotics, will provide the Russian military with better nutrition in the extreme conditions of the Arctic.

Kajian fizikokimia terhadap Trigon sp. Madu Lebah Kelulut di Daerah Johor

Johor as one of the states of Malaysia is a good geographic location for meliponiculture activity. In this study, ten samples of stingless bee honey from each regions of Johor and one sample obtained from Institute Bioproduct Development, Universiti Teknologi Malaysia, Skudai were analyzed for the physicochemical properties of stingless bee honey. The physicochemical analyses were including moisture, total soluble solids, ash, pH, free acidity, conductivity, hydroxymethylfurfural (HMF) content, protein, carbohydrate, fat, dietary fibre, total phenolic content (TPC), total flavonoid content (TFC), and DPPH radical scavenging activity. Trigona sp. sample from Kluang have the highest value of phenolic content (778.23 ± 2.011 mg GAE/100 g) while Trigona sp. sample from Mersing have the highest value of flavonoid content (194.98 ± 0.350 mg RE/100 g). Among eleven samples tested for radical scavenging activity, Trigona sp. sample from Kota Tinggi have the highest scavenging activity (23.37 ± 0.36 mg/ml), but the value of HMF content exceeded the limit of Malaysian standard for stingless bee honey. All parameters were significantly different (p &lt; 0.01) except for ash. This study showed a strong correlation between moisture and acidity (r = 0.601). However, low correlation was obtained between TPC and TFC with DPPH radical scavenging activity (r = -0.235, 0.011). The data obtained from this study could help for a better subsequent of Malaysian stingless bee honey industry.

Assessing the environmental sustainability of glucose from wheat as a fermentation feedstock

In recent years, the need to substitute fossil fuels with renewable biomass has been a key driver in the development of the biorefinery concept. One of the possible routes towards the development of bioproducts under this scheme is through a sugar intermediate. Sugars, such as glucose, can be produced through starch crops, for instance wheat. While there are many environmental assessment studies that consider sugar as a platform for biofuel production, the main focus is on the end product of the value chain (typically bioethanol), but not on sugars as the basic feedstock. Taking the bottom-up perspective as a roadmap, the assessment of technological, economic and environmental barriers in the biorefinery scheme must take into account the sustainability of sugar production with the aim of improving its current framework or finding novel technologies. This study investigates the environmental sustainability of wheat cultivation and grain processing in different European countries by applying the life cycle assessment (LCA) methodology with a cradle to gate approach. Moreover, 1 kg of glucose at the factory gate has been considered as a functional unit for reporting the environmental results. The chosen impact categories are climate change (CC), particular matter (PM), human toxicity (HT), freshwater eutrophication (FE), terrestrial eutrophication (TE), acidification (AC) and abiotic depletion (AD). Mass and economic allocations are evaluated as the processing of grain wheat generates different valuable by-products, namely wheat bran, gluten meal and gluten feed. The results show that agricultural activities play an important role in the environmental impacts, predominantly due to the production of agrochemicals and field emissions derived from fertilisation. In addition, the choice of allocation has a greater environmental influence (both positive or negative) on the by-products than the main product under investigation.

Environment and Bioeconomics: An Analysis of the Brazilian Ecosystem in Bioproducts Development

Environment and Bioeconomics: An Analysis of the Brazilian Ecosystem in Bioproducts Development

Effect of CO2 and metal-rich waste water on bioproduct potential of the diazotrophic freshwater cyanobacterium, Tolypothrix sp.

Continued economic growth is reliant on stable, affordable energy, requiring at present fossil fuel-derived energy production. Coal-fired power stations produce metal-rich but macro-nutrient-poor waste waters and emit flue gas, containing ∼10% CO2. Algae and cyanobacteria remediate metals and CO2, but use of N2-fixing (diazotrophic) cyanobacteria can reduce nitrogen-fertilization costs. The resulting biomass represents a promising source for biofuel and bio-product development. This study investigated the effect of CO2- and trace metals on growth performance, biochemical profiles and metal content of the freshwater diazotrophic cyanobacterium Tolypothrix sp. to assess bioproduct potential. Aerated 2 L batch cultures were grown in simulated ash-dam water (SADW) and BG11 without nitrogen (BG11(-N) controls). Supplied air was supplemented with either 15% CO2 or not (non-CO2 controls). CO2 supplementation resulted in 2.4 and 3.3-fold higher biomass productivities and 1.3 and 1.2-fold higher phycocyanin and phycoerythrin contents, whilst metals (media) had no effect. Al, Cu, Ni and V were more efficiently removed (50–90%) with CO2-addition, while As, Mo, Se and Sr removal was higher (30–87%) for non-CO2 controls. No significant effect on Zn and Fe removal was evident. Calculated biomass metal concentrations, at quantities required to meet N-requirements of wheat, suggests no metal toxicity when applied as a mineral-nitrogen biofertilizer. With a carbohydrate content of 50%, the biomass is also suitable for bioethanol production. In summary, Tolypothrix sp. raised in ash dam waste water supplemented with flue gas CO2 could yield high-value phycobiliproteins, bioethanol or biogas, and mineral-rich nitrogen fertilizer which would offset remediation costs and improve agricultural productivity.

Product and Microbial Population Kinetics During Balsamic-Styled Vinegar Production.

Balsamic-styled vinegar is a nutraceutical product obtained from a two-stage fermentation process of grape must. However, little is known about how fermentation conditions affect growth kinetics, bio-product development, population dynamics and the final product quality. As a result, the current study investigated the effect of fermentation temperature and inoculation strategy on the fermentation dynamics of Balsamic-styled vinegar production. A microbial consortium of non-Saccharomyces yeasts (n = 13) and acetic acid bacteria (n = 5) was tested at various fermentation temperatures (22°C, 28°C and a fluctuating temperature regimen). Different inoculation strategies (co-inoculation and sequential inoculation) were investigated, and population dynamics of the product selected due to a rapid fermentation period were confirmed using a 16S and 18S gene sequencing. A higher fermentation temperature (28°C) and co-inoculation strategy resulted in a shorter fermentation cycle, whilst the desired acetic acid concentration of 60 g/L was achieved within 38 days. 16S and 18S gene sequencing showed that 50.84% of Acetobacter species were abundant at the end of the fermentation cycle, while 40.18% bacteria were unculturable. The study provides a better understanding of how fermentation temperature and inoculation strategy affect the fermentation period, population dynamics and the growth kinetics of the microbial consortium during the production of Balsamic-styled vinegar. PRACTICAL APPLICATION: Lower quality South African wine grapes could be channelled to an alternative high-priced product (Balsamic-styled vinegar), with low technological input requirements. Thus, making it easier to incorporate a low capital start-up business while empowering small business entrepreneurs and boosting the economy.