Abstract

Adequate formulation of bioproducts represents one of the most challenging aspects of bioproduct development. The incorporation of adjuvants with bioagents can positively influence product development. However, it is indispensable to evaluate the sensitivity of bioagents to these adjuvants. The aim of this study was to determine the toxicity of seven adjuvants at different concentrations to Bipolaris yamadae (Bipolaris euphorbiae) fungus, to select a product compatible with this phytopathogen for a wettable powder formulation and to evaluate the pathogenicity of the formulation against wild poinsettia (Euphorbia heterophylla). The powder fraction of the formulation was made up of 1% anti-wetting silicon dioxide mixed with B. yamadae conidia to a final concentration of 107 conidia.ml-1. The aqueous fraction was composed of 0.1% Geropon T36 compatibilizer, 0.075% silicone, the dispersant, 0.1% Tween 80 or tensioactive and 0.5% PVP K30 or spreading agent. The incidence of disease was observed in 83.6% of the plants inoculated with the formulated fungus, which was 79.0% higher than that in the plants inoculated with the bioagent only. These findings strongly suggest that the new formulation successfully controls Euphorbia heterophylla and greatly increases the pathogenicity of the fungus.   Key words: Bipolaris yamadae, Euphorbia heterophylla, adjuvants, biological control, phytopathogenic fungi.

Highlights

  • Weed control plays an important role in the management of economically important crops (Green, 2014; Zhu et al, 2020)

  • The spread of weeds with biotypes that areresistant to chemical herbicides, concerns about environmental issues and the necessity of reducing production costs are the main factors that drive the search for new weed control strategies (Caldwell et al, 2012)

  • Anti-wetting silicon dioxide was previously identified by Machado et al (2016) as a wettable powder for B. yamadae bioformulation due to its texture, which allows an increase in the preparation volume of conidia + antiwetting and creates a homogeneous mixture

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Summary

Introduction

Weed control plays an important role in the management of economically important crops (Green, 2014; Zhu et al, 2020). The spread of weeds with biotypes that areresistant to chemical herbicides, concerns about environmental issues and the necessity of reducing production costs are the main factors that drive the search for new weed control strategies (Caldwell et al, 2012). The use of specific phytopathogens as bioherbicides is a potential strategy for weed management due to its practicality and environmental safety.

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