Biomimetic Characteristics Research Articles

Mechanical and Corrosion Behaviour in Simulated Body Fluid of As-Fabricated 3D Porous L-PBF 316L Stainless Steel Structures for Biomedical Implants.

Laser powder bed fusion (L-PBF) is one of the most promising additive manufacturing technologies for creating customised 316L Stainless Steel (SS) implants with biomimetic characteristics, controlled porosity, and optimal structural and functional properties. However, the behaviour of as-fabricated 3D 316L SS structures without any surface finishing in environments that simulate body fluids remains largely unknown. To address this knowledge gap, the present study investigates the surface characteristics, the internal porosity, the corrosion in simulated body fluid (SBF), and the mechanical properties of as-fabricated 316L SS structures manufactured by L-PBF with rhombitruncated cuboctahedron (RTCO) unit cells with two distinct relative densities (10 and 35%). The microstructural analysis confirmed that the RTCO structure has a pure austenitic phase with a roughness of ~20 µm and a fine cellular morphology. The micro-CT revealed the presence of keyholes and a lack of fusion pores in both RTCO structures. Despite the difference in the internal porosity, the mechanical properties of both structures remain within the range of bone tissue and in line with the Gibson and Ashby model. Additionally, the as-fabricated RTCO structures demonstrated passive corrosion behaviour in the SBF solution. Thus, as-fabricated porous structures are promising biomaterials for implants due to their suitable surface roughness, mechanical properties, and corrosion resistance, facilitating bone tissue growth.

Full‐vdW Heterosynaptic Memtransistor with the Ferroelectric Inserted Functional Layer and its Neuromorphic Applications

AbstractThe emergence of 2D van der Waals (vdW) materials, owing to highly tunable electrical conductivity, remarkably free stackability, and excellent compatibility for heterojunction integration, has provided abundant research diversity of artificial synapses. Here, an innovative 2D vdW heterosynaptic memtransistor (vdW‐HT) synapse is proposed with a ferroelectric CuInP2S6 inserted layer. Neuromorphic synaptic weight change of the vdW‐HT synapse in this work are modulated by the synergistic effects of interlayer coupling and ferroelectric polarization reversal. It is the first time to evaluate the required initial energy consumption of ferroelectric vdW‐HT synapses with matrixed biomimetic characteristics of “Learning–Forgetting–Re‐learning–Memorizing.” The required initial energy consumption is only ≈3.06 pJ, which provided supporting evidence to indicate the promising potential of vdW‐HT synapses in exploring neuromorphic applications. A vdW‐HT computing system with artificial recognition capability for intelligent automobiles is established and demonstrated outstanding recognition abilities for multiple targets in various environments. The highest recognition accuracy for pedestrians is 98%. In addition, the excellent recognition property is integrated with a robotic arm, successfully achieving high‐precision grasping behavior and designated position transmission for identified targets. These results provide promising strategies for the integrated development of emerging neuromorphic electronics and industrial applications.

Conductive Polymer-Based Hydrogels for Wearable Electrochemical Biosensors.

Hydrogels are gaining popularity for use in wearable electronics owing to their inherent biomimetic characteristics, flexible physicochemical properties, and excellent biocompatibility. Among various hydrogels, conductive polymer-based hydrogels (CP HGs) have emerged as excellent candidates for future wearable sensor designs. These hydrogels can attain desired properties through various tuning strategies extending from molecular design to microstructural configuration. However, significant challenges remain, such as the limited strain-sensing range, significant hysteresis of sensing signals, dehydration-induced functional failure, and surface/interfacial malfunction during manufacturing/processing. This review summarizes the recent developments in polymer-hydrogel-based wearable electrochemical biosensors over the past five years. Initially serving as carriers for biomolecules, polymer-hydrogel-based sensors have advanced to encompass a wider range of applications, including the development of non-enzymatic sensors facilitated by the integration of nanomaterials such as metals, metal oxides, and carbon-based materials. Beyond the numerous existing reports that primarily focus on biomolecule detection, we extend the scope to include the fabrication of nanocomposite conductive polymer hydrogels and explore their varied conductivity mechanisms in electrochemical sensing applications. This comprehensive evaluation is instrumental in determining the readiness of these polymer hydrogels for point-of-care translation and state-of-the-art applications in wearable electrochemical sensing technology.

The third dimension of tumor microenvironment-The importance of tumor stroma in 3D cancer models.

Recent advances in the three-dimensional (3D) cancer models give rise to a plethora of new possibilities in the development of anti-cancer drug therapies and bring us closer to personalized medicine. Three-dimensional models are undoubtedly more authentic than traditional two-dimensional (2D) cell cultures. Nowadays, they are becoming preferentially used in most cancer research fields due to their more accurate biomimetic characteristics. On the contrary, they still lack the cellular and matrix complexity of the native tumor microenvironment (TME). This review focuses on the description of existing 3D models, the incorporation of TME and fluidics into these models, and their perspective in the future research. It is clear that such an improvement would need not only biological but also technical progress. Therefore, the modern approach to anti-cancer drug discovery should involve various fields.

Mussel-derived Bioadaptive Artificial Tendon Facilitates the Cell Proliferation and Tenogenesis to Promote Tendon Functional Reconstruction.

Tendon injuries range from acute-related trauma to chronic-related injuries are prevalent and bring substantial pain, functional loss, and even disability to the patients. The management of tendon injuries is tricky due to the innate limited regenerative capability of the tendon. Currently, surgical intervention of tendon injuries with artificial tendons remains the standard of care. However, most of artificial tendons are manufactured with synthetic materials, which possess relatively poor biomimetic characteristics and inadequate inherent biodegradability, hence rendering limited cell proliferation and migration for tendon healing. To address these limitations, w e developed a mussel-derived artificial tendon based on double-crosslinked chitosan modification. In this design, decellularized artificial tendon serves as a natural biomimetic scaffold to facilitate the migration and adhesion of tendon repair cells. Additionally, as the cells proliferate, the artificial tendon can be degraded to facilitate tendon regeneration. Moreover, the chitosan cross-linking further enhances the mechanical strength of artificial tendon and offers a controllable degradation. The in vitro and in vivo experimental results demonstrated that mussel-derived artificial tendon not only accelerated the tendon functional reconstruction but also enabled harmless clearance at post-implantation. O ur finding provides a promising alternative to conventional artificial tendons and spurs a new frontier to explore nature-derived artificial tendons. This article is protected by copyright. All rights reserved.

Development of hybrid scaffolds with biodegradable polymer composites and bioactive hydrogels for bone tissue engineering

The development of treatments for critical-sized bone defects has been considered an important topic in the biomedical field because of the high demand for transplantable bone grafts. Following the concept of tissue engineering, implantation of biocompatible porous scaffolds carrying cells and regulating factors is the most efficient strategy to stimulate clinical bone regeneration. With the advancement in the development of 3D-printing techniques, scaffolds with highly controllable architectures can be fabricated to further improve healing efficacies. However, challenges such as the limited biocompatibility of resin materials and poor cell-carrying capacities still exist in the application of current scaffolds. In this study, a novel biodegradable polymer, poly (ethylene glycol)-co-poly (glycerol sebacate) acrylate (PEGSA), was synthesized and blended with hydroxyapatite (HAP) nanoparticles to produce osteoinductive and photocurable resins for 3D printing. The composites were optimized and applied in the fabrication of gyroid scaffolds with biomimetic characteristics and high permeability, followed by the combination of bioactive hydrogels containing Wharton's jelly-derived mesenchymal stem cells (WJMSC) to increase the efficiency of cell delivery. The promotion of osteogenesis from 3D-printed scaffolds was confirmed in-vivo while the hybrid scaffolds were proven to be great platforms for WJMSC culture and differentiation in-vitro. These results indicate that the proposed hybrid systems, combining osteoinductive 3D-printed scaffolds and cell-laden hydrogels, have great potential for bone tissue engineering and are expected to be applied in the treatment of bone defects based on active tissue regeneration.

Research on Regional Architectural Design Method Based on GIS

Urbanization and continued population growth have had a major impact on the urban environment. Many buildings lack close regional and biomimetic characteristics during the rapid generation process, making it impossible to achieve ecological sustainability. This article took the rehabilitation center of Jinghong Dai Cultural Park in Yunnan as an example for research, intending to highlight regional characteristics and change the current severely homogenized urban style. Visual on-site environmental analysis was performed through Arc GIS, CFD-phoenics, and sketchup, and the site form planning of ecological buildings from the perspective of “regional characteristics” was explored. Morphological data of local buildings and regional plants were collected, plant growth patterns were analyzed in Grasshopper software, and skins were generated from the perspective of “biomimetic properties”, and we combined the planning form with the skin to form the overall regional architectural design. The design approach of multi-parameter software combinations brings a richer expression morphologically and distinguishes the more homogeneous stereotypical image of the city, which is of great significance and value in expanding the form of architectural design. The spatial form of the site layout and the phenology of the native plants respect the original natural environment and create a symbiosis between man and nature.

High-speed CMOS-free purely spintronic asynchronous recurrent neural network

The exceptional capabilities of the human brain provide inspiration for artificially intelligent hardware that mimics both the function and the structure of neurobiology. In particular, the recent development of nanodevices with biomimetic characteristics promises to enable the development of neuromorphic architectures with exceptional computational efficiency. In this work, we propose biomimetic neurons comprised of domain wall-magnetic tunnel junctions that can be integrated into the first trainable CMOS-free recurrent neural network with biomimetic components. This paper demonstrates the computational effectiveness of this system for benchmark tasks and its superior computational efficiency relative to alternative approaches for recurrent neural networks.

Mechanical and Biomimetic Characteristics of Bulk-Fill Resin Dental Composites Following Exposure in a Simulated Acidic Oral Environment

During the last 10 years, various companies have marketed different "bulk-fill" resin dental composites for the restoration of posterior stress-bearing teeth; however, the impact of acidic conditions on these relatively newer materials has not been thoroughly investigated. Therefore, an attempt was made to evaluate the effect of acidic beverages on the mechanical biomimetic characteristics of four bulk-fill and one conventional nanohybrid resin-based dental composites (RBCs). The specimens of each RBC were stored in two acidic beverages namely 'Orange Juice' and 'Coca-Cola', whereas 'dry' and 'distilled water' storage of specimens served as controls. After 1 week of storage, flexural and surface hardness properties of specimens were determined using a universal testing machine and Vickers hardness tester, respectively. In general, the 'Coca-Cola' beverage caused the greatest degradation of flexural strength, flexural modulus, and surface hardness characteristics in all RBCs in contrast to the 'dry', 'distilled water' controls and 'Orange Juice' storage conditions. However, the overall mechanical biomimetic performance of nanohybrid RBCs was relatively better than all other bulk-fill RBCs and may, therefore, be considered a suitable candidate for the restoration of posterior stress-bearing permanent dentition.

Facile construction of a 3D tumor model with multiple biomimetic characteristics using a micropatterned chip for large-scale chemotherapy investigation.

The establishment and application of biomimetic preclinical tumor models for generalizable and high-throughput antitumor screening play a promising role in drug discovery and cancer therapeutics. Herein, a facile and robust microengineering-assisted methodology for highly biomimetic three-dimensional (3D) tumor construction for dynamic and large-scale antitumor investigation is developed using micropatterned array chips. The high fidelity, simplicity, and stability of chip fabrication are guaranteed by improved polydimethylsiloxane (PDMS) microcontact printing. The employment of a PDMS-micropatterned chip permits microscale, simple, biocompatible, and reproducible cell localization with quantity uniformity and 3D tumor array formation with geometric homogeneity. Array-like 3D tumor models possessing complex multilayer cell arrangements, diverse phenotypic gradients, and biochemical gradients were prepared based on the use of easy-to-operate chips. The applicability of the established biomimetic models in temporal and massive investigations of tumor responses to antitumor chemotherapy is also verified experimentally. The results support the importance of the dimensional geometry and biomimetic degree of 3D tumors when conducting antitumor screening to explore drug susceptibility and resistance. This work provides a facile and reliable strategy to perform highly biomimetic tumor manipulation and analysis, which holds great potential for applications in oncology, pharmacology, precision medicine, and tissue microengineering.

Design and mechanical properties analysis of heterogeneous porous scaffolds based on bone slice images.

Bone tissue engineering plays an extremely important role in the clinical treatment of bone defects. Porous scaffold is one of the three essential factors of bone tissue engineering, and its structural design has attracted more and more attention . At present, most of the design methods of porous scaffolds focus on uniform porous scaffolds with periodic and regular pore structures. However, periodic and regular pore structure cannot comprehensively and accurately simulate the microstructures and mechanical properties of natural bone. To address this problem, based on bone slice images and VT (Voronoi-Tessellation) method, this article proposed a design method of HPS (Heterogeneous Porous Scaffolds) with bionic pore structure and controllable porosity. The FDM (fused deposition modeling) printing technology was applied to fabricate HPS with different porosities, and the mechanical properties of the HPS were analyzed by experiments. The research results illustrate that the HPS constructed by the design method proposed in this article have good controllability, and their internal pore structures are highly similar to those of natural bone, which have biomimetic characteristics. The mechanical property analysis illustrate that the stiffness and compressive strength of HPS decrease with the increase of porosity, in addition, the heterogeneous pore distribution makes HPS have the characteristics of non-concentrated and discontinuous damage distribution. This study provides a new idea for the design of porous scaffolds and a theoretical basis for the bionic design of HPS.

Additive manufacturing of metallic biomaterials and its biocompatibility

The techniques for making orthopaedic implants and devices have advanced significantly in recent years. The use of additive manufacturing technology enables the creation of intricate structures with biomimetic characteristics. Three-dimensional (3D) printed titanium orthopaedic implants were already used to repair severe pelvic bone abnormalities, and the production of thousands of non-individualized implants. The various adjustable variables in the 3D printing process and how they could interact with the potential variety of designs that might be produced must be understood in order to predict the efficacy and safety of AM made implants. A reasonably well-known technology known as powder bed 3D printing has revolutionised the process of creating personalised, multipurpose metallic implants for each patient's unique demands. Precise mechanical characteristics with porous implants, good pore topologies, and patient-oriented design functionalities can be created via AM. Although 3D printed Ti and its alloy Ti-6Al-4V have been studied and used in clinical applications from several years, further study is still needed to build porous AM titanium implants that would operate superbly over the long term. Although early clinical outcomes for 3D printed cups have been promising, additional extensive study is still needed, because regulatory approval systems have not fully kept up with technological innovation. For various applications, a variety of 3D printing methods, including selective laser melting, selective laser sintering, and electron beam melting, are discussed in this paper. The production of excellent porous metal implants has a lot of potential for additive manufacturing using powder bed fusion.

Design of the Jump Mechanism for a Biomimetic Robotic Frog.

Frogs are vertebrate amphibians with both efficient swimming and jumping abilities due to their well-developed hind legs. They can jump over obstacles that are many or even tens of times their size on land. However, most of the current jumping mechanisms of biomimetic robotic frogs use simple four-bar linkage mechanisms, which has an unsatisfactory biomimetic effect on the appearance and movement characteristics of frogs. At the same time, multi-joint jumping robots with biomimetic characteristics are subject to high drive power requirements for jumping action. In this paper, a novel jumping mechanism of a biomimetic robotic frog is proposed. Firstly, the structural design of the forelimb and hindlimb of the frog is given, and the hindlimb of the robotic frog is optimized based on the design of a single-degree-of-freedom six-bar linkage. A simplified model is established to simulate the jumping motion. Secondly, a spring energy storage and trigger mechanism is designed, including incomplete gear, one-way bearing, torsion spring, and so on, to realize the complete jumping function of the robot, that is, elastic energy storage and regulation, elastic energy release, and rapid leg retraction. Thirdly, the experimental prototype of the biomimetic robotic frog is fabricated. Finally, the rationality and feasibility of the jumping mechanism are verified by a jumping experiment. This work provides a technical and theoretical basis for the design and development of a high-performance amphibious biomimetic robotic frog.

Smart membranes for biomedical applications

Smart membranes with tunable permeability and selectivity have drawn widespread attention because of their unique biomimetic characteristics. Constructed by incorporating various stimuli-responsive materials into membrane substrates, smart membranes could self-adjust their physical/chemical properties (such as pore size and surface properties) in response to environmental signals such as temperature, pH, light, magnetic field, electric field, redox and specific ions/molecules. Such smart membranes show great prospects in biomedical applications ranging from controlled drug release to bioseparation and tissue engineering. In this review, three controlled release models realized by different designed smart membranes are emphatically introduced, and then smart membranes for biological separation and controlled cell culture are introduced and discussed respectively. At last, the existing challenges of smart membranes for biomedical applications are briefly summarized, and future research topics are suggested.

Cold Spraying of Thick Biomimetic and Stoichiometric Apatite Coatings for Orthopaedic Implants

Ceramic coatings have a long history in the orthopaedic field, with plasma sprayed coatings of hydroxyapatite as leading standard in the manufacturing process; however, these coatings can contain secondary phases resulting from the decomposition of hydroxyapatite at high temperatures, which limit the lifetime of implants and their osseointegration. This work aims to produce coatings that can maximize bone osseointegration of metallic implants. In order to preserve the raw characteristics of hydroxyapatite powders that are thermally unstable, coatings were deposited by cold spray onto Ti6Al4V alloy substrates. In contrast with other thermal spray technologies, this process presents the advantage of spraying particles through a supersonic gas jet at a low temperature. On top of hydroxyapatite, carbonated nanocrystalline apatite was synthesized and sprayed. This biomimetic apatite is similar to bone minerals due to the presence of carbonates and its poor crystallinity. FTIR and XRD analyses proved that the biomimetic characteristics and the non-stoichiometric of the apatite were preserved in the cold spray coatings. The cold spray process did not affect the chemistry of the raw material. The adhesion of the coatings as well as their thicknesses were evaluated, showing values comparable to conventional process. Cold spraying appears as a promising method to preserve the characteristics of calcium phosphate ceramics and to produce coatings that offer potentially improved osseointegration.

Biomimetic Strain-Stiffening in Chitosan Self-Healing Hydrogels.

The strain-stiffening and self-healing capabilities of biological tissues enable them to preserve the structures and functions from deformation and damage. However, biodegradable hydrogel materials with both of these biomimetic characteristics have not been explored. Here, a series of strain-stiffened, self-healing hydrogels are developed through dynamic imine crosslinking of semiflexible O-carboxymethyl chitosan (main chain) and flexible dibenzaldehyde-terminated telechelic poly(ethylene glycol) (crosslinker). The biomimetic hydrogels can be reversibly stiffened to resist the deformation and can even recover to their original state after repeated damages. The mechanical properties and stiffening responses of the hydrogels are tailored by varying the component contents (1-3%) and the crosslinker length (4 or 8 kDa). A combinatorial system of in situ coherent small-angle X-ray scattering with rheological testing is developed to investigate the network structures (in sizes 1.5-160 nm) of hydrogels under shear strains and reveals that the strain-stiffening originates from the fibrous chitosan network with poly(ethylene glycol) crosslinking fixation. The biomimetic hydrogels with biocompatibility and biodegradability promote wound healing. The study provides an insight into the nanoscale design of biomimetic strain-stiffening self-healing hydrogels for biomedical applications.

Analysis of structural and biomimetic characteristics of the green-synthesized Fe3O4 nanozyme from the fruit peel extract of Punica granatum

Analysis of structural and biomimetic characteristics of the green-synthesized Fe3O4 nanozyme from the fruit peel extract of Punica granatum

Electron Beam-Treated Enzymatically Mineralized Gelatin Hydrogels for Bone Tissue Engineering.

Biological hydrogels are highly promising materials for bone tissue engineering (BTE) due to their high biocompatibility and biomimetic characteristics. However, for advanced and customized BTE, precise tools for material stabilization and tuning material properties are desired while optimal mineralisation must be ensured. Therefore, reagent-free crosslinking techniques such as high energy electron beam treatment promise effective material modifications without formation of cytotoxic by-products. In the case of the hydrogel gelatin, electron beam crosslinking further induces thermal stability enabling biomedical application at physiological temperatures. In the case of enzymatic mineralisation, induced by Alkaline Phosphatase (ALP) and mediated by Calcium Glycerophosphate (CaGP), it is necessary to investigate if electron beam treatment before mineralisation has an influence on the enzymatic activity and thus affects the mineralisation process. The presented study investigates electron beam-treated gelatin hydrogels with previously incorporated ALP and successive mineralisation via incubation in a medium containing CaGP. It could be shown that electron beam treatment optimally maintains enzymatic activity of ALP which allows mineralisation. Furthermore, the precise tuning of material properties such as increasing compressive modulus is possible. This study characterizes the mineralised hydrogels in terms of mineral formation and demonstrates the formation of CaP in dependence of ALP concentration and electron dose. Furthermore, investigations of uniaxial compression stability indicate increased compression moduli for mineralised electron beam-treated gelatin hydrogels. In summary, electron beam-treated mineralized gelatin hydrogels reveal good cytocompatibility for MG-63 osteoblast like cells indicating a high potential for BTE applications.

Polymeric Hydrogel Scaffolds: Skin Tissue Engineering and Regeneration.

Tissue engineering is a novel regenerative approach in the medicinal field that promises the regeneration of damaged tissues. Moreover, tissue engineering involves synthetic and natural biomaterials that facilitate tissue or organ growth outside the body. Not surprisingly, the demand for polymer-based therapeutical approaches in skin tissue defects has increased at an effective rate, despite the pressing clinical need. Among the 3D scaffolds for tissue engineering and regeneration approaches, hydrogel scaffolds have shown significant importance for their use as 3D cross-linked scaffolds in skin tissue regeneration due to their ideal moisture retention property and porosity biocompatibility, biodegradable, and biomimetic characteristics. In this review, we demonstrated the choice of ideal biomaterials to fabricate the novel hydrogel scaffolds for skin tissue engineering. After a short introduction to the bioactive and drug-loaded polymeric hydrogels, the discussion turns to fabrication and characterisation techniques of the polymeric hydrogel scaffolds. In conclusion, we discuss the excellent wound healing potential of stem cell-loaded hydrogels and Nano-based approaches to designing hydrogel scaffolds for skin tissue engineering.

Porcine Decellularized Diaphragm Hydrogel: A New Option for Skeletal Muscle Malformations.

Hydrogels are biomaterials that, thanks to their unique hydrophilic and biomimetic characteristics, are used to support cell growth and attachment and promote tissue regeneration. The use of decellularized extracellular matrix (dECM) from different tissues or organs significantly demonstrated to be far superior to other types of hydrogel since it recapitulates the native tissue’s ECM composition and bioactivity. Different muscle injuries and malformations require the application of patches or fillers to replenish the defect and boost tissue regeneration. Herein, we develop, produce, and characterize a porcine diaphragmatic dECM-derived hydrogel for diaphragmatic applications. We obtain a tissue-specific biomaterial able to mimic the complex structure of skeletal muscle ECM; we characterize hydrogel properties in terms of biomechanical properties, biocompatibility, and adaptability for in vivo applications. Lastly, we demonstrate that dECM-derived hydrogel obtained from porcine diaphragms can represent a useful biological product for diaphragmatic muscle defect repair when used as relevant acellular stand-alone patch.